MysmA.10520.a

Hydrolase (putative beta-lactamase)

CENTER ID: MysmA.10520.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.10520.a.A1.GE28983 Full length( MysmA.10520.a ) 1 234
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.10520.a.A1.PS00685 Full length( MysmA.10520.a ) 1 234

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3748
RefSeq: YP_888103.1
UniProt: A0QYW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTPSETPSSI TVDDTYTGHV EPGTAARRTL ADATIVKASV GPMDNNCYLV TCRSTGETLL IDAANDAEVI IGLVEQHAPK VSLIVTTHQH QDHWLALQAV TEATGAPTAA HALDAGALPV KPDRILSDGD KITVGNLTFD AIHLQGHTPG SVALALAGAD GADGPTHLFT GDCLFPGGVG KTWKPGDFET LLGDVTTKVF DVYDDRTVVY PGHGDDTTLG AERPSLAEWR ERGW
NT Sequence
ATGACCCCAT CTGAGACGCC ATCCAGCATC ACGGTCGACG ACACCTACAC CGGACACGTC GAGCCAGGAA CCGCAGCGCG GCGCACCCTC GCCGACGCCA CGATCGTCAA GGCGTCGGTG GGTCCCATGG ACAACAACTG CTACCTGGTG ACCTGCAGAT CTACCGGCGA GACGCTGCTG ATCGACGCGG CCAACGACGC CGAGGTGATC ATCGGACTCG TCGAGCAGCA CGCGCCGAAG GTGTCACTGA TCGTCACCAC CCATCAACAT CAGGACCACT GGCTCGCGCT GCAGGCCGTG ACAGAAGCCA CAGGAGCCCC GACCGCCGCG CATGCGCTCG ACGCCGGCGC GTTGCCCGTC AAGCCGGACC GGATCCTGTC CGACGGCGAC AAGATCACCG TCGGCAACCT CACTTTCGAC GCCATCCACC TACAGGGCCA CACGCCCGGT TCGGTGGCAC TGGCCCTCGC GGGTGCCGAT GGCGCCGACG GGCCCACGCA CCTGTTCACC GGCGACTGCC TGTTCCCGGG CGGCGTCGGC AAGACGTGGA AGCCGGGCGA CTTCGAGACG CTGCTGGGCG ACGTCACCAC CAAGGTGTTC GACGTCTACG ACGACCGCAC GGTCGTCTAC CCCGGGCACG GTGACGACAC CACTCTCGGC GCGGAGCGTC CGAGCCTGGC CGAGTGGCGT GAACGGGGCT GG
Details for MysmA.10520.a.A1.GE28983
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 26kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSETPSS ITVDDTYTGH VEPGTAARRT LADATIVKAS VGPMDNNCYL VTCRSTGETL LIDAANDAEV IIGLVEQHAP KVSLIVTTHQ HQDHWLALQA VTEATGAPTA AHALDAGALP VKPDRILSDG DKITVGNLTF DAIHLQGHTP GSVALALAGA DGADGPTHLF TGDCLFPGGV GKTWKPGDFE TLLGDVTTKV FDVYDDRTVV YPGHGDDTTL GAERPSLAEW RERGW
Validated NT Sequence
ttattaccag ccccgttcac gccactcggc caggctcgga cgctccgcgc cgagagtggt gtcgtcaccg tgcccggggt agacgaccgt gcggtcgtcg tagacgtcga acaccttggt ggtgacgtcg cccagcagcg tctcgaagtc gcccggcttc cacgtcttgc cgacgccgcc cgggaacagg cagtcgccgg tgaacaggtg cgtgggcccg tcggcgccat cggcacccgc gagggccagt gccaccgaac cgggcgtgtg gccctgtagg tggatggcgt cgaaagtgag gttgccgacg gtgatcttgt cgccgtcgga caggatccgg tccggcttga cgggcaacgc gccggcgtcg agcgcatgcg cggcggtcgg ggctcctgtg gcttctgtca cggcctgcag cgcgagccag tggtcctgat gttgatgggt ggtgacgatc agtgacacct tcggcgcgtg ctgctcgacg agtccgatga tcacctcggc gtcgttggcc gcgtcgatca gcagcgtctc gccggtagat ctgcaggtca ccaggtagca gttgttgtcc atgggaccca ccgacgcctt gacgatcgtg gcgtcggcga gggtgcgccg cgctgcggtt cctggctcga cgtgtccggt gtaggtgtcg tcgaccgtga tgctggatgg cgtctcagat ggggtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSETPSS ITVDDTYTGH VEPGTAARRT LADATIVKAS VGPMDNNCYL VTCRSTGETL LIDAANDAEV IIGLVEQHAP KVSLIVTTHQ HQDHWLALQA VTEATGAPTA AHALDAGALP VKPDRILSDG DKITVGNLTF DAIHLQGHTP GSVALALAGA DGADGPTHLF TGDCLFPGGV GKTWKPGDFE TLLGDVTTKV FDVYDDRTVV YPGHGDDTTL GAERPSLAEW RERGW
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccccatct gagacgccat ccagcatcac ggtcgacgac acctacaccg gacacgtcga gccaggaacc gcagcgcggc gcaccctcgc cgacgccacg atcgtcaagg cgtcggtggg tcccatggac aacaactgct acctggtgac ctgcagatct accggcgaga cgctgctgat cgacgcggcc aacgacgccg aggtgatcat cggactcgtc gagcagcacg cgccgaaggt gtcactgatc gtcaccaccc atcaacatca ggaccactgg ctcgcgctgc aggccgtgac agaagccaca ggagccccga ccgccgcgca tgcgctcgac gccggcgcgt tgcccgtcaa gccggaccgg atcctgtccg acggcgacaa gatcaccgtc ggcaacctca ctttcgacgc catccaccta cagggccaca cgcccggttc ggtggcactg gccctcgcgg gtgccgatgg cgccgacggg cccacgcacc tgttcaccgg cgactgcctg ttcccgggcg gcgtcggcaa gacgtggaag ccgggcgact tcgagacgct gctgggcgac gtcaccacca aggtgttcga cgtctacgac gaccgcacgg tcgtctaccc cgggcacggt gacgacacca ctctcggcgc ggagcgtccg agcctggccg agtggcgtga acggggctgg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for MysmA.10520.a.A1.PS00685
PURIFICATION DATe: 7/14/2010
CONCENTRATION: 40.39mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSETPSS ITVDDTYTGH VEPGTAARRT LADATIVKAS VGPMDNNCYL VTCRSTGETL LIDAANDAEV IIGLVEQHAP KVSLIVTTHQ HQDHWLALQA VTEATGAPTA AHALDAGALP VKPDRILSDG DKITVGNLTF DAIHLQGHTP GSVALALAGA DGADGPTHLF TGDCLFPGGV GKTWKPGDFE TLLGDVTTKV FDVYDDRTVV YPGHGDDTTL GAERPSLAEW RERGW
Validated NT Sequence
ttattaccag ccccgttcac gccactcggc caggctcgga cgctccgcgc cgagagtggt gtcgtcaccg tgcccggggt agacgaccgt gcggtcgtcg tagacgtcga acaccttggt ggtgacgtcg cccagcagcg tctcgaagtc gcccggcttc cacgtcttgc cgacgccgcc cgggaacagg cagtcgccgg tgaacaggtg cgtgggcccg tcggcgccat cggcacccgc gagggccagt gccaccgaac cgggcgtgtg gccctgtagg tggatggcgt cgaaagtgag gttgccgacg gtgatcttgt cgccgtcgga caggatccgg tccggcttga cgggcaacgc gccggcgtcg agcgcatgcg cggcggtcgg ggctcctgtg gcttctgtca cggcctgcag cgcgagccag tggtcctgat gttgatgggt ggtgacgatc agtgacacct tcggcgcgtg ctgctcgacg agtccgatga tcacctcggc gtcgttggcc gcgtcgatca gcagcgtctc gccggtagat ctgcaggtca ccaggtagca gttgttgtcc atgggaccca ccgacgcctt gacgatcgtg gcgtcggcga gggtgcgccg cgctgcggtt cctggctcga cgtgtccggt gtaggtgtcg tcgaccgtga tgctggatgg cgtctcagat ggggtcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTPSETPSS ITVDDTYTGH VEPGTAARRT LADATIVKAS VGPMDNNCYL VTCRSTGETL LIDAANDAEV IIGLVEQHAP KVSLIVTTHQ HQDHWLALQA VTEATGAPTA AHALDAGALP VKPDRILSDG DKITVGNLTF DAIHLQGHTP GSVALALAGA DGADGPTHLF TGDCLFPGGV GKTWKPGDFE TLLGDVTTKV FDVYDDRTVV YPGHGDDTTL GAERPSLAEW RERGW
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCCCATCT GAGACGCCAT CCAGCATCAC GGTCGACGAC ACCTACACCG GACACGTCGA GCCAGGAACC GCAGCGCGGC GCACCCTCGC CGACGCCACG ATCGTCAAGG CGTCGGTGGG TCCCATGGAC AACAACTGCT ACCTGGTGAC CTGCAGATCT ACCGGCGAGA CGCTGCTGAT CGACGCGGCC AACGACGCCG AGGTGATCAT CGGACTCGTC GAGCAGCACG CGCCGAAGGT GTCACTGATC GTCACCACCC ATCAACATCA GGACCACTGG CTCGCGCTGC AGGCCGTGAC AGAAGCCACA GGAGCCCCGA CCGCCGCGCA TGCGCTCGAC GCCGGCGCGT TGCCCGTCAA GCCGGACCGG ATCCTGTCCG ACGGCGACAA GATCACCGTC GGCAACCTCA CTTTCGACGC CATCCACCTA CAGGGCCACA CGCCCGGTTC GGTGGCACTG GCCCTCGCGG GTGCCGATGG CGCCGACGGG CCCACGCACC TGTTCACCGG CGACTGCCTG TTCCCGGGCG GCGTCGGCAA GACGTGGAAG CCGGGCGACT TCGAGACGCT GCTGGGCGAC GTCACCACCA AGGTGTTCGA CGTCTACGAC GACCGCACGG TCGTCTACCC CGGGCACGGT GACGACACCA CTCTCGGCGC GGAGCGTCCG AGCCTGGCCG AGTGGCGTGA ACGGGGCTGG AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA