MysmA.01613.k

Acyl-CoA dehydrogenase family protein

CENTER ID: MysmA.01613.k
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01613.k.A1.PS01016 Full length( MysmA.01613.k ) 1 410

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2158
RefSeq: YP_886545.1
UniProt: A0QUF7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLKWSKTDQ EFRDEVRGFL AEKLTPDLRE AGRLQTSVYS EHEASMEWQR ILHERGWAAP AWPVEHGGCD WSLTQHYIFS RESTLAGAPA LSPMGIRMVA HAIIKFGTEE QKSYFLPRIL TGEVFFCQGY SEPEAGSDLA ALSMSAVSDG DDLVCTGSKI WTTHAQEANW MFALVRTTRG AKKQQGITFV LIDMTSPGIQ IRPLVMTSGE EVQNQVFFDE VRVPKKNVIG AIDDGWTVAK YLLEFERGGG ASSPALQVLG EEIATVAARQ PGPSGGRLID DPAFSRKLAD ARIRTDVLEI LEYQVLAAVA EGRNPGTASS MLKVLSTELS QILTELSMEA AGPRGRAYQP HATRPGGPVA EFEPPADGYV SGEPWQAVAP LRYFNDRAGS IYAGSNEIQR NILAKAALGL
NT Sequence
ATGGACCTGA AATGGTCGAA AACCGACCAG GAGTTCCGCG ACGAGGTGCG CGGCTTCCTC GCGGAGAAAC TGACACCCGA CCTCCGCGAG GCGGGACGGC TCCAGACGAG CGTGTACTCC GAGCACGAAG CGAGCATGGA ATGGCAGCGC ATCCTGCACG AACGCGGATG GGCGGCCCCG GCGTGGCCGG TCGAGCACGG CGGCTGCGAC TGGAGCCTGA CGCAGCACTA CATCTTCAGC CGCGAGTCCA CGCTCGCCGG CGCACCGGCG CTGTCCCCGA TGGGCATCCG CATGGTGGCG CACGCCATCA TCAAGTTCGG CACCGAGGAG CAGAAGTCCT ACTTCCTCCC CCGCATCCTG ACCGGCGAGG TGTTCTTCTG CCAGGGCTAC TCCGAACCCG AGGCCGGTTC GGACCTCGCG GCGCTGTCGA TGAGCGCGGT CTCCGACGGA GACGACCTGG TCTGCACCGG CAGCAAGATC TGGACCACGC ATGCGCAGGA AGCGAACTGG ATGTTCGCGC TCGTGCGCAC CACCCGCGGT GCCAAGAAGC AGCAGGGCAT CACGTTCGTG TTGATCGACA TGACCTCGCC CGGTATCCAG ATCCGCCCCC TGGTGATGAC CTCGGGTGAA GAGGTGCAGA ACCAGGTGTT CTTCGACGAG GTGCGGGTGC CGAAGAAGAA CGTCATCGGC GCCATCGACG ACGGCTGGAC CGTCGCCAAG TACCTGCTGG AGTTCGAACG CGGCGGCGGC GCATCCTCAC CCGCGCTGCA GGTGCTCGGC GAGGAGATCG CGACCGTGGC CGCGCGGCAG CCGGGCCCGT CCGGGGGGCG GCTGATCGAC GATCCGGCGT TCAGCCGCAA GCTCGCCGAC GCCCGCATCC GCACCGACGT CCTGGAGATC CTGGAGTACC AGGTGCTCGC GGCGGTCGCC GAGGGCCGCA ATCCCGGCAC CGCGTCGTCG ATGCTCAAGG TGCTCAGCAC CGAGCTGAGC CAGATTCTCA CCGAGCTGTC GATGGAGGCC GCCGGTCCAC GCGGCCGCGC CTACCAGCCG CACGCGACGC GTCCGGGTGG TCCGGTCGCC GAGTTCGAGC CGCCCGCCGA CGGTTATGTC AGCGGTGAAC CGTGGCAGGC CGTCGCACCG CTGCGCTACT TCAACGACCG CGCCGGGTCG ATCTACGCCG GCAGCAACGA GATTCAGCGG AACATCCTCG CCAAAGCGGC CCTCGGACTC
Details for MysmA.01613.k.A1.PS01016
PURIFICATION DATe: 3/29/2011
CONCENTRATION: 17.14mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 61
Protocol Notes
notes unavailable
Validated AA Sequence
MSAVSDGDDL VCTGSKIWTT HAQEANWMFA LVRTTRGAKK QQGITFVLID MTSPGIQIRP LVMTSGEEVQ NQVFFDEVRV PKKNVIGAID DGWTVAKYLL EFERGGGASS PALQVLGEEI ATVAARQPGP SGGRLIDDPA FSRKLADARI RTDVLEILEY QVLAAVAEGR NPGTASSMLK VLSTELSQIL TELSMEAAGP RGRAYQPHAT RPGGPVAEFE PPADGYVSGE PWQAVAPLRY FNDRAGSIYA GSNEIQRNIL AKAALGL
Validated NT Sequence
cnnntnnnnn nnntgttagc agccggatcc tcgagaagct tggctgcaga acttgttcgt gctgtttatt agagtccgag ggccgctttg gcgaggatgt tccgctgaat ctcgttgctg ccggcgtaga tcgacccggc gcggtcgttg aagtagcgca gcggtgcgac ggcctgccac ggttcaccgc tgacataacc gtcggcgggc ggctcgaact cggcgaccgg accacccgga cgcgtcgcgt gcggctggta ggcgcggccg cgtggaccgg cggcctccat cgacagctcg gtgagaatct ggctcagctc ggtgctgagc accttgagca tcgacgacgc ggtgccggga ttgcggccct cggcgaccgc cgcgagcacc tggtactcca ggatctccag gacgtcggtg cggatgcggg cgtcggcgag cttgcggctg aacgccggat cgtcgatcag ccgccccccg gacgggcccg gctgccgcgc ggccacggtc gcgatctcct cgccgagcac ctgcagcgcg ggtgaggatg cgccgccgcc gcgttcgaac tccagcaggt acttggcgac ggtccagccg tcgtcgatgg cgccgatgac gttcttcttc ggcacccgca cctcgtcgaa gaacacctgg ttctgcacct cttcacccga ggtcatcacc agggggcgga tctggatacc gggcgaggtc atgtcgatca acacgaacgt gatgccctgc tgcttcttgg caccgcgggt ggtgcgcacg agcgcgaaca tccagttcgc ttcctgcgca tgcgtggtcc agatcttgct gccggtgcag accaggtcgt ctccgtcgga gaccgcgctc atcgacagcg ccgcgaggtc cgaaccgggc ctcgggttcg gagtagccct ggcagaagaa cacctcgncg gtcagnatgc gggggangaa gtngnacttc tgctcctcgn ngccgaantt gatgatggcg tgcgccaccn tgcnnatgcc catcggggnn nncnncgcgn nnnnnnnnng nctcncngnt nnnntgnann gntgnnncnn nncnntnnna ncnnccnnnn tngannnnnn nnnnnnnnnn ncnnnnccnn ntnnnnnnnn nnnnnnnnnn nnnnnnnnnt nnnntnnnng gnnnngnnnn nnnnnnnncn nnnnnnnnnn nnnnnnnnnn nnnnc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLKWSKTD QEFRDEVRGF LAEKLTPDLR EAGRLQTSVY SEHEASMEWQ RILHERGWAA PAWPVEHGGC DWSLTQHYIF SRESTLAGAP ALSPMGIRMV AHAIIKFGTE EQKSYFLPRI LTGEVFFCQG YSEPEAGSDL AALSMSAVSD GDDLVCTGSK IWTTHAQEAN WMFALVRTTR GAKKQQGITF VLIDMTSPGI QIRPLVMTSG EEVQNQVFFD EVRVPKKNVI GAIDDGWTVA KYLLEFERGG GASSPALQVL GEEIATVAAR QPGPSGGRLI DDPAFSRKLA DARIRTDVLE ILEYQVLAAV AEGRNPGTAS SMLKVLSTEL SQILTELSME AAGPRGRAYQ PHATRPGGPV AEFEPPADGY VSGEPWQAVA PLRYFNDRAG SIYAGSNEIQ RNILAKAALG L
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACCTGAAA TGGTCGAAAA CCGACCAGGA GTTCCGCGAC GAGGTGCGCG GCTTCCTCGC GGAGAAACTG ACACCCGACC TCCGCGAGGC GGGACGGCTC CAGACGAGCG TGTACTCCGA GCACGAAGCG AGCATGGAAT GGCAGCGCAT CCTGCACGAA CGCGGATGGG CGGCCCCGGC GTGGCCGGTC GAGCACGGCG GCTGCGACTG GAGCCTGACG CAGCACTACA TCTTCAGCCG CGAGTCCACG CTCGCCGGCG CACCGGCGCT GTCCCCGATG GGCATCCGCA TGGTGGCGCA CGCCATCATC AAGTTCGGCA CCGAGGAGCA GAAGTCCTAC TTCCTCCCCC GCATCCTGAC CGGCGAGGTG TTCTTCTGCC AGGGCTACTC CGAACCCGAG GCCGGTTCGG ACCTCGCGGC GCTGTCGATG AGCGCGGTCT CCGACGGAGA CGACCTGGTC TGCACCGGCA GCAAGATCTG GACCACGCAT GCGCAGGAAG CGAACTGGAT GTTCGCGCTC GTGCGCACCA CCCGCGGTGC CAAGAAGCAG CAGGGCATCA CGTTCGTGTT GATCGACATG ACCTCGCCCG GTATCCAGAT CCGCCCCCTG GTGATGACCT CGGGTGAAGA GGTGCAGAAC CAGGTGTTCT TCGACGAGGT GCGGGTGCCG AAGAAGAACG TCATCGGCGC CATCGACGAC GGCTGGACCG TCGCCAAGTA CCTGCTGGAG TTCGAACGCG GCGGCGGCGC ATCCTCACCC GCGCTGCAGG TGCTCGGCGA GGAGATCGCG ACCGTGGCCG CGCGGCAGCC GGGCCCGTCC GGGGGGCGGC TGATCGACGA TCCGGCGTTC AGCCGCAAGC TCGCCGACGC CCGCATCCGC ACCGACGTCC TGGAGATCCT GGAGTACCAG GTGCTCGCGG CGGTCGCCGA GGGCCGCAAT CCCGGCACCG CGTCGTCGAT GCTCAAGGTG CTCAGCACCG AGCTGAGCCA GATTCTCACC GAGCTGTCGA TGGAGGCCGC CGGTCCACGC GGCCGCGCCT ACCAGCCGCA CGCGACGCGT CCGGGTGGTC CGGTCGCCGA GTTCGAGCCG CCCGCCGACG GTTATGTCAG CGGTGAACCG TGGCAGGCCG TCGCACCGCT GCGCTACTTC AACGACCGCG CCGGGTCGAT CTACGCCGGC AGCAACGAGA TTCAGCGGAA CATCCTCGCC AAAGCGGCCC TCGGACTCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA