MysmA.01613.i

Acyl-CoA dehydrogenase

CENTER ID: MysmA.01613.i
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01613.i.A1.GE28966 Full length( MysmA.01613.i ) 1 396
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01613.i.A1.PS00848 Full length( MysmA.01613.i ) 1 396

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6408
RefSeq: YP_890796.1
UniProt: A0R6K8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSVAEADLV AERARQVVSE HDPKSVPIPQ FLAACYDAGL SWVHFPEGMG GLGVSRGLQA VVDRILQGAG GPVPLGLNPM GYGMAAPTIR EHAQNDELKK SWLRPLASTE HIWCQLFSEP GAGSDLAGLA TSAVPDGDDW VINGQKVWTS LAHRARWGLL LARTNPDVPK HKGLTYFVID MHGPGVETRP LRQMTGQAEF NEVYMTDARI PDAHRLGAVG DGWRVAMTTL MNERSALGGS GSRRGAGTIA DAVSLWASRP DLHTPVLRDR LTQLWLRSEA QRLTAERSRA TATVGGPGPE GSIGKLVGAE LNQHIYQWCM DFLGPEGLLY DGYGLGDGDG ADWRGPVQQR FLRSKANTIE GGTSEVMRNI LGERILGLPG DLRADAGMAW KEIPRG
NT Sequence
ATGAGCAGCG TCGCTGAAGC GGACCTGGTG GCCGAGCGGG CCCGCCAGGT CGTCTCCGAG CATGACCCGA AAAGCGTTCC GATACCGCAG TTCCTCGCCG CCTGCTACGA CGCGGGACTG TCCTGGGTGC ATTTCCCCGA GGGCATGGGC GGTCTCGGTG TGTCGCGCGG CCTGCAGGCC GTGGTCGATC GCATCCTGCA GGGCGCCGGA GGGCCGGTCC CGCTGGGGCT CAACCCGATG GGCTACGGCA TGGCCGCGCC CACGATCCGC GAGCATGCGC AGAACGACGA GCTCAAGAAA TCCTGGCTGC GTCCGCTGGC GAGCACCGAG CACATCTGGT GCCAGCTGTT CTCCGAGCCG GGTGCGGGTT CGGACCTGGC GGGCCTGGCC ACCTCGGCCG TGCCGGACGG CGACGACTGG GTGATCAACG GCCAGAAGGT GTGGACCAGC CTGGCGCACC GGGCACGGTG GGGTCTGCTG CTGGCGCGCA CGAATCCCGA TGTGCCCAAG CACAAAGGAC TCACGTACTT CGTGATCGAC ATGCACGGAC CCGGCGTCGA GACCCGGCCG CTGCGCCAGA TGACGGGTCA GGCCGAGTTC AACGAGGTCT ACATGACCGA CGCCCGCATT CCCGATGCGC ACCGTCTCGG TGCCGTAGGC GACGGCTGGC GGGTCGCGAT GACGACGCTG ATGAACGAGC GCAGCGCGCT CGGCGGCAGC GGCAGCCGGC GAGGCGCGGG CACCATCGCC GACGCGGTGT CGCTGTGGGC GTCGCGTCCG GATCTGCACA CCCCGGTGCT GCGTGACCGG TTGACGCAAC TGTGGTTGCG CTCGGAGGCG CAGCGCCTCA CCGCCGAACG CTCGCGCGCG ACCGCCACGG TCGGCGGTCC CGGACCCGAG GGGTCCATCG GCAAGCTCGT GGGTGCGGAG CTCAACCAGC ACATCTACCA GTGGTGCATG GATTTCCTTG GCCCGGAGGG TCTTCTGTAC GACGGTTACG GGCTGGGCGA CGGTGATGGC GCCGACTGGC GCGGACCCGT GCAGCAGCGG TTCCTGCGCA GCAAGGCCAA CACCATCGAA GGAGGCACGT CCGAGGTGAT GCGCAACATT CTCGGTGAAC GGATCCTGGG GTTGCCCGGG GATCTGCGGG CGGATGCCGG TATGGCGTGG AAGGAGATCC CGCGTGGC
Details for MysmA.01613.i.A1.GE28966
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 94
PERCENT COVERAGE: 94
Validated AA Sequence
MSXVXEADLV AERARQVVSE HDPKSVPIPX FLAACYDAXL SWVHFPEGMG GLGXXRGLQA VVDRILQGAG XXVPLGXNPX GYGXXAPTIR EXAXNDXXXK SWLXXLASXE HIWCQLFSEP GAXSDLAGLA TSAVPDGDDW VINGQKVWTS LAHRARWGLL LARTNPDVPK HKGLTYFVID MHGPGVETRP LRQMTGQAEF NEVYMTDARI PDAHRLGAVG DGWRVAMTTL MNERSALGGS GSRRGAGTIA DAVSLWASRP DLHTPVLRDR LTQLWLRSEA QRLTAERSRA TATVGGPGPE GSIGKLVGAE LNQHIYQWCM DFLGPEGLLY DGYGLGDGDG ADWRGPVQQR FLRSKANTIE GGTSEVMRNI LGERILGLPG DLRADAGMAW KEIPRG
Validated NT Sequence
ttattagcca cgcgggatct ccttccacgc cataccggca tccgcccgca gatccccggg caaccccagg atccgttcac cgagaatgtt gcgcatcacc tcggacgtgc ctccttcgat ggtgttggcc ttgctgcgca ggaaccgctg ctgcacgggt ccgcgccagt cggcgccatc accgtcgccc agcccgtaac cgtcgtacag aagaccctcc gggccaagga aatccatgca ccactggtag atgtgctggt tgagctccgc acccacgagc ttgccgatgg acccctcggg tccgggaccg ccgaccgtgg cggtcgcgcg cgagcgttcg gcggtgaggc gctgcgcctc cgagcgcaac cacagttgcg tcaaccggtc acgcagcacc ggggtgtgca gatccggacg cgacgcccac agcgacaccg cgtcggcgat ggtgcccgcg cctcgccggc tgccgctgcc gccgagcgcg ctgcgctcgt tcatcagcgt cgtcatcgcg acccgccagc cgtcgcctac ggcaccgaga cggtgcgcat cgggaatgcg ggcgtcggtc atgtagacct cgttgaactc ggcctgaccc gtcatctggc gcagcggccg ggtctcgacg ccgggtccgt gcatgtcgat cacgaagtac gtgagtcctt tgtgcttggg cacatcggga ttcgtgcgcg ccagcagcag accccaccgt gcccggtgcg ccaggctggt ccacaccttc tggccgttga tcacccagtc gtcgccgtcc ggcacggccg aggtggccag gcccgccagg tccgaanccg cacccggctc ggagaacagc tggcaccaga tgtgctcngn gctcgccagc ngnngcagcc aggatttnnn ganntngtcg ttcngngcan gctcgcggat ngtgggngcg nccannccgt agcccnnngg gttgannccc agcgggaccg nccntccggc gccctgcagg atgcgatcga ccacggcctg caggccgcgn gncncnccga gaccgcccat gccctcgggg aaatgcaccc aggacagtcn cgcgtcgtag caggcggcga ggaantgcgg tatcggaacg cttttcgggt catgctcgga gacgacctgg cgggcccgct cggccaccag gtccgcttca nngacgntgc tcatcgaacc aggaccctgg gtctgagctn nc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSVAEADL VAERARQVVS EHDPKSVPIP QFLAACYDAG LSWVHFPEGM GGLGVSRGLQ AVVDRILQGA GGPVPLGLNP MGYGMAAPTI REHAQNDELK KSWLRPLAST EHIWCQLFSE PGAGSDLAGL ATSAVPDGDD WVINGQKVWT SLAHRARWGL LLARTNPDVP KHKGLTYFVI DMHGPGVETR PLRQMTGQAE FNEVYMTDAR IPDAHRLGAV GDGWRVAMTT LMNERSALGG SGSRRGAGTI ADAVSLWASR PDLHTPVLRD RLTQLWLRSE AQRLTAERSR ATATVGGPGP EGSIGKLVGA ELNQHIYQWC MDFLGPEGLL YDGYGLGDGD GADWRGPVQQ RFLRSKANTI EGGTSEVMRN ILGERILGLP GDLRADAGMA WKEIPRG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcagcgtc gctgaagcgg acctggtggc cgagcgggcc cgccaggtcg tctccgagca tgacccgaaa agcgttccga taccgcagtt cctcgccgcc tgctacgacg cgggactgtc ctgggtgcat ttccccgagg gcatgggcgg tctcggtgtg tcgcgcggcc tgcaggccgt ggtcgatcgc atcctgcagg gcgccggagg gccggtcccg ctggggctca acccgatggg ctacggcatg gccgcgccca cgatccgcga gcatgcgcag aacgacgagc tcaagaaatc ctggctgcgt ccgctggcga gcaccgagca catctggtgc cagctgttct ccgagccggg tgcgggttcg gacctggcgg gcctggccac ctcggccgtg ccggacggcg acgactgggt gatcaacggc cagaaggtgt ggaccagcct ggcgcaccgg gcacggtggg gtctgctgct ggcgcgcacg aatcccgatg tgcccaagca caaaggactc acgtacttcg tgatcgacat gcacggaccc ggcgtcgaga cccggccgct gcgccagatg acgggtcagg ccgagttcaa cgaggtctac atgaccgacg cccgcattcc cgatgcgcac cgtctcggtg ccgtaggcga cggctggcgg gtcgcgatga cgacgctgat gaacgagcgc agcgcgctcg gcggcagcgg cagccggcga ggcgcgggca ccatcgccga cgcggtgtcg ctgtgggcgt cgcgtccgga tctgcacacc ccggtgctgc gtgaccggtt gacgcaactg tggttgcgct cggaggcgca gcgcctcacc gccgaacgct cgcgcgcgac cgccacggtc ggcggtcccg gacccgaggg gtccatcggc aagctcgtgg gtgcggagct caaccagcac atctaccagt ggtgcatgga tttccttggc ccggagggtc ttctgtacga cggttacggg ctgggcgacg gtgatggcgc cgactggcgc ggacccgtgc agcagcggtt cctgcgcagc aaggccaaca ccatcgaagg aggcacgtcc gaggtgatgc gcaacattct cggtgaacgg atcctggggt tgcccgggga tctgcgggcg gatgccggta tggcgtggaa ggagatcccg cgtggcaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MysmA.01613.i.A1.PS00848
PURIFICATION DATe: 11/11/2010
CONCENTRATION: 45.13mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 94
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MSXVXEADLV AERARQVVSE HDPKSVPIPX FLAACYDAXL SWVHFPEGMG GLGXXRGLQA VVDRILQGAG XXVPLGXNPX GYGXXAPTIR EXAXNDXXXK SWLXXLASXE HIWCQLFSEP GAXSDLAGLA TSAVPDGDDW VINGQKVWTS LAHRARWGLL LARTNPDVPK HKGLTYFVID MHGPGVETRP LRQMTGQAEF NEVYMTDARI PDAHRLGAVG DGWRVAMTTL MNERSALGGS GSRRGAGTIA DAVSLWASRP DLHTPVLRDR LTQLWLRSEA QRLTAERSRA TATVGGPGPE GSIGKLVGAE LNQHIYQWCM DFLGPEGLLY DGYGLGDGDG ADWRGPVQQR FLRSKANTIE GGTSEVMRNI LGERILGLPG DLRADAGMAW KEIPRG
Validated NT Sequence
ttattagcca cgcgggatct ccttccacgc cataccggca tccgcccgca gatccccggg caaccccagg atccgttcac cgagaatgtt gcgcatcacc tcggacgtgc ctccttcgat ggtgttggcc ttgctgcgca ggaaccgctg ctgcacgggt ccgcgccagt cggcgccatc accgtcgccc agcccgtaac cgtcgtacag aagaccctcc gggccaagga aatccatgca ccactggtag atgtgctggt tgagctccgc acccacgagc ttgccgatgg acccctcggg tccgggaccg ccgaccgtgg cggtcgcgcg cgagcgttcg gcggtgaggc gctgcgcctc cgagcgcaac cacagttgcg tcaaccggtc acgcagcacc ggggtgtgca gatccggacg cgacgcccac agcgacaccg cgtcggcgat ggtgcccgcg cctcgccggc tgccgctgcc gccgagcgcg ctgcgctcgt tcatcagcgt cgtcatcgcg acccgccagc cgtcgcctac ggcaccgaga cggtgcgcat cgggaatgcg ggcgtcggtc atgtagacct cgttgaactc ggcctgaccc gtcatctggc gcagcggccg ggtctcgacg ccgggtccgt gcatgtcgat cacgaagtac gtgagtcctt tgtgcttggg cacatcggga ttcgtgcgcg ccagcagcag accccaccgt gcccggtgcg ccaggctggt ccacaccttc tggccgttga tcacccagtc gtcgccgtcc ggcacggccg aggtggccag gcccgccagg tccgaanccg cacccggctc ggagaacagc tggcaccaga tgtgctcngn gctcgccagc ngnngcagcc aggatttnnn ganntngtcg ttcngngcan gctcgcggat ngtgggngcg nccannccgt agcccnnngg gttgannccc agcgggaccg nccntccggc gccctgcagg atgcgatcga ccacggcctg caggccgcgn gncncnccga gaccgcccat gccctcgggg aaatgcaccc aggacagtcn cgcgtcgtag caggcggcga ggaantgcgg tatcggaacg cttttcgggt catgctcgga gacgacctgg cgggcccgct cggccaccag gtccgcttca nngacgntgc tcatcgaacc aggaccctgg gtctgagctn nc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSSVAEADL VAERARQVVS EHDPKSVPIP QFLAACYDAG LSWVHFPEGM GGLGVSRGLQ AVVDRILQGA GGPVPLGLNP MGYGMAAPTI REHAQNDELK KSWLRPLAST EHIWCQLFSE PGAGSDLAGL ATSAVPDGDD WVINGQKVWT SLAHRARWGL LLARTNPDVP KHKGLTYFVI DMHGPGVETR PLRQMTGQAE FNEVYMTDAR IPDAHRLGAV GDGWRVAMTT LMNERSALGG SGSRRGAGTI ADAVSLWASR PDLHTPVLRD RLTQLWLRSE AQRLTAERSR ATATVGGPGP EGSIGKLVGA ELNQHIYQWC MDFLGPEGLL YDGYGLGDGD GADWRGPVQQ RFLRSKANTI EGGTSEVMRN ILGERILGLP GDLRADAGMA WKEIPRG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCAGCGTC GCTGAAGCGG ACCTGGTGGC CGAGCGGGCC CGCCAGGTCG TCTCCGAGCA TGACCCGAAA AGCGTTCCGA TACCGCAGTT CCTCGCCGCC TGCTACGACG CGGGACTGTC CTGGGTGCAT TTCCCCGAGG GCATGGGCGG TCTCGGTGTG TCGCGCGGCC TGCAGGCCGT GGTCGATCGC ATCCTGCAGG GCGCCGGAGG GCCGGTCCCG CTGGGGCTCA ACCCGATGGG CTACGGCATG GCCGCGCCCA CGATCCGCGA GCATGCGCAG AACGACGAGC TCAAGAAATC CTGGCTGCGT CCGCTGGCGA GCACCGAGCA CATCTGGTGC CAGCTGTTCT CCGAGCCGGG TGCGGGTTCG GACCTGGCGG GCCTGGCCAC CTCGGCCGTG CCGGACGGCG ACGACTGGGT GATCAACGGC CAGAAGGTGT GGACCAGCCT GGCGCACCGG GCACGGTGGG GTCTGCTGCT GGCGCGCACG AATCCCGATG TGCCCAAGCA CAAAGGACTC ACGTACTTCG TGATCGACAT GCACGGACCC GGCGTCGAGA CCCGGCCGCT GCGCCAGATG ACGGGTCAGG CCGAGTTCAA CGAGGTCTAC ATGACCGACG CCCGCATTCC CGATGCGCAC CGTCTCGGTG CCGTAGGCGA CGGCTGGCGG GTCGCGATGA CGACGCTGAT GAACGAGCGC AGCGCGCTCG GCGGCAGCGG CAGCCGGCGA GGCGCGGGCA CCATCGCCGA CGCGGTGTCG CTGTGGGCGT CGCGTCCGGA TCTGCACACC CCGGTGCTGC GTGACCGGTT GACGCAACTG TGGTTGCGCT CGGAGGCGCA GCGCCTCACC GCCGAACGCT CGCGCGCGAC CGCCACGGTC GGCGGTCCCG GACCCGAGGG GTCCATCGGC AAGCTCGTGG GTGCGGAGCT CAACCAGCAC ATCTACCAGT GGTGCATGGA TTTCCTTGGC CCGGAGGGTC TTCTGTACGA CGGTTACGGG CTGGGCGACG GTGATGGCGC CGACTGGCGC GGACCCGTGC AGCAGCGGTT CCTGCGCAGC AAGGCCAACA CCATCGAAGG AGGCACGTCC GAGGTGATGC GCAACATTCT CGGTGAACGG ATCCTGGGGT TGCCCGGGGA TCTGCGGGCG GATGCCGGTA TGGCGTGGAA GGAGATCCCG CGTGGCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA