SSGCID: Target Page

MysmA.01613.d

Acyl-CoA dehydrogenase domain protein

CENTER ID:	MysmA.01613.d
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01613.d.A1.GE28961	Full length( MysmA.01613.d )		1	391

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01613.d.A1.PS00879	Full length( MysmA.01613.d )		1	391

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5475
RefSeq:	YP_889853.1
UniProt:	A0R3W5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTHPENLDDF RATVRDWCAA HVPKDWRAAQ TGVSDDEFAA FQKAWFAELH SAGYAVPHWP AEWGGGMSVP QQIVLYSELA AHDAPRLVLA FVGIHHAAST LLAAGTDAQR NRHLPAILDG EIWVQGFSEP EAGSDLASLR TTARAEGDTF VVNGQKLWAS GAMYADWCLL LARTDPNAPK RHGISYFMMD MKTPGVDVRP IRNAVGDSHF CEIFLNDVVI PSANLVGPVN KGWQVAQATL GAERGMTMLE LAERLGNAGF RWLVDACSPV DDPVVADRLA QFEIELTGLR GLCRDLVERS ETGEAGPADA SIVKLYYSEL LQRMTDFGAQ IAGLPAHTVL TKPASSGWES GAWMLDFIGS WEWTIPGGAS EIQRTIIGER GLGLPREPSA V

NT Sequence

GTGACCCACC CCGAAAACTT GGACGACTTC CGCGCCACGG TGCGCGACTG GTGCGCCGCG CACGTCCCCA AAGACTGGCG CGCGGCCCAA ACCGGCGTGA GCGACGACGA ATTCGCGGCG TTCCAGAAGG CGTGGTTCGC CGAGTTGCAC AGCGCCGGCT ACGCCGTACC GCACTGGCCC GCCGAGTGGG GCGGCGGCAT GTCGGTTCCG CAGCAGATCG TGCTGTACTC CGAACTCGCG GCACACGACG CGCCCCGCCT CGTACTGGCG TTCGTCGGCA TCCACCACGC CGCGTCGACG CTGCTCGCCG CGGGCACAGA CGCACAGCGC AACCGGCACC TGCCCGCGAT CCTCGACGGC GAGATCTGGG TACAGGGCTT CTCCGAACCC GAGGCCGGTT CGGATCTGGC GTCGCTGCGC ACCACGGCCC GCGCCGAGGG CGACACCTTC GTGGTCAACG GGCAGAAGCT GTGGGCCAGT GGAGCCATGT ACGCCGACTG GTGCCTGCTG CTGGCCCGCA CCGACCCGAA TGCCCCCAAA CGCCACGGCA TTTCCTACTT CATGATGGAC ATGAAGACGC CGGGTGTCGA TGTGCGTCCG ATCCGCAACG CGGTCGGCGA TTCGCACTTC TGCGAGATCT TCCTCAACGA CGTCGTGATC CCGTCGGCCA ATCTCGTCGG CCCGGTCAAC AAGGGCTGGC AGGTCGCGCA GGCGACGCTG GGCGCCGAAC GCGGAATGAC CATGCTCGAA CTCGCCGAAC GTCTCGGCAA TGCGGGGTTC CGCTGGCTCG TGGACGCCTG CTCGCCCGTG GATGATCCGG TCGTGGCGGA TCGCCTGGCG CAGTTCGAGA TCGAGCTCAC CGGTTTGCGC GGACTGTGCC GCGACCTGGT CGAACGCAGC GAAACCGGTG AGGCGGGCCC AGCCGACGCC TCGATCGTCA AGCTGTACTA CAGCGAACTG TTGCAACGGA TGACCGATTT CGGCGCCCAG ATCGCCGGGC TGCCCGCGCA CACCGTGCTG ACCAAACCCG CGTCGAGCGG GTGGGAATCG GGGGCGTGGA TGCTCGACTT CATCGGCTCT TGGGAATGGA CCATCCCCGG CGGGGCCAGC GAGATCCAGC GCACCATCAT CGGTGAGCGG GGTTTGGGCC TGCCGCGAGA GCCGAGTGCG GTG

Details for MysmA.01613.d.A1.GE28961

HARVESTED ON:	3/30/2010
SEQUENCED ON:	4/20/2010
EXPECTED MW:	44kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	3
PERCENT COVERAGE:	44

Validated AA Sequence

MLLTKXXXXX XXXXSQXXXX XXXXGXXXXX TXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXYXXLPX XXXXXXXXXX XXXXXPXSXX XXXXXXXXXX XXXXXXXPXX XXXXXXXXXX XXXXXXXXXX XPXXXXXXXX IXXXXXXXXX XXXXXXXXXL XXXXXXXXXX XXXXXRXXXX XXI

Validated NT Sequence

ttntgtnnnn nttnnagann tnccntggnt catcaccntc accatcnnnn nnnnnnnnnn ngctcagann nnnggtcctg gttcgatggt gannnnnnnn nnaaacttgg acgacttccg cgccacggtg cgcgactggt gcgccgcgca cgtccccaaa gactggcgcg cggnccaaac cggngngagc gacnatnant tcnnnnngnt ccanaaggnn ngnttcgncn agttgnanag ngncggntac gccnnancgn nctggnncgn nnagtggnnc ggcggaatgn cngntcnnna ntanatnnng nngnacnnnn tnntcgnngn nnnnnnnncg nncnnnctcg nnnngnnnnt ngnngnatcn nnnannnngn nanaatgctg ctgacnaaga ngnnnnnntg nnnnnnnnnn ngangttcnc aanncnnnan nngnnnnnnn nnnnntggtn nnnnnatnnn cangacntnt nnnnnnnnnn nntnnnntnc nnnnaangnn ncnnnnncng nnanngntng gnnnnnnnnn cnngnngnnn ngnnatnnna cnnannnnng nnganttacn gcancttacc tnnnnnnnnn nnnnnnnnct ntnnnncnnt nnnnnnnnnn ncnnntnanc cnnnttcann nnatnnnnnt nnnnnnnnnn nnnnnnnnnn nnnnnngnnt nngnnncnan ntcnnccnnn tannnngncn nnnnnnnnnn nnnngnnnnt nnnnntncnn nnnnnnatng nnnnacncng nnnatncccn nnnnnnnngc nttgnnnnnn cannatcnnn ncnnnnnnnn ngtnnanang nnannagnna nntnnannnn ncnnnnnnnn nctnnnnnnn cnnnaannnn tnnnncnnna aanannannn cnnannnnnc ggtngngnnn tnncnnnnnn ataatc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVTHPENLD DFRATVRDWC AAHVPKDWRA AQTGVSDDEF AAFQKAWFAE LHSAGYAVPH WPAEWGGGMS VPQQIVLYSE LAAHDAPRLV LAFVGIHHAA STLLAAGTDA QRNRHLPAIL DGEIWVQGFS EPEAGSDLAS LRTTARAEGD TFVVNGQKLW ASGAMYADWC LLLARTDPNA PKRHGISYFM MDMKTPGVDV RPIRNAVGDS HFCEIFLNDV VIPSANLVGP VNKGWQVAQA TLGAERGMTM LELAERLGNA GFRWLVDACS PVDDPVVADR LAQFEIELTG LRGLCRDLVE RSETGEAGPA DASIVKLYYS ELLQRMTDFG AQIAGLPAHT VLTKPASSGW ESGAWMLDFI GSWEWTIPGG ASEIQRTIIG ERGLGLPREP SAV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgacccac cccgaaaact tggacgactt ccgcgccacg gtgcgcgact ggtgcgccgc gcacgtcccc aaagactggc gcgcggccca aaccggcgtg agcgacgacg aattcgcggc gttccagaag gcgtggttcg ccgagttgca cagcgccggc tacgccgtac cgcactggcc cgccgagtgg ggcggcggca tgtcggttcc gcagcagatc gtgctgtact ccgaactcgc ggcacacgac gcgccccgcc tcgtactggc gttcgtcggc atccaccacg ccgcgtcgac gctgctcgcc gcgggcacag acgcacagcg caaccggcac ctgcccgcga tcctcgacgg cgagatctgg gtacagggct tctccgaacc cgaggccggt tcggatctgg cgtcgctgcg caccacggcc cgcgccgagg gcgacacctt cgtggtcaac gggcagaagc tgtgggccag tggagccatg tacgccgact ggtgcctgct gctggcccgc accgacccga atgcccccaa acgccacggc atttcctact tcatgatgga catgaagacg ccgggtgtcg atgtgcgtcc gatccgcaac gcggtcggcg attcgcactt ctgcgagatc ttcctcaacg acgtcgtgat cccgtcggcc aatctcgtcg gcccggtcaa caagggctgg caggtcgcgc aggcgacgct gggcgccgaa cgcggaatga ccatgctcga actcgccgaa cgtctcggca atgcggggtt ccgctggctc gtggacgcct gctcgcccgt ggatgatccg gtcgtggcgg atcgcctggc gcagttcgag atcgagctca ccggtttgcg cggactgtgc cgcgacctgg tcgaacgcag cgaaaccggt gaggcgggcc cagccgacgc ctcgatcgtc aagctgtact acagcgaact gttgcaacgg atgaccgatt tcggcgccca gatcgccggg ctgcccgcgc acaccgtgct gaccaaaccc gcgtcgagcg ggtgggaatc gggggcgtgg atgctcgact tcatcggctc ttgggaatgg accatccccg gcggggccag cgagatccag cgcaccatca tcggtgagcg gggtttgggc ctgccgcgag agccgagtgc ggtgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MysmA.01613.d.A1.PS00879

PURIFICATION DATe:	11/22/2010
CONCENTRATION:	28.39mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	3
PERCENT COVERAGE:	44

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCCAC CCCGAAAACT TGGACGACTT CCGCGCCACG GTGCGCGACT GGTGCGCCGC GCACGTCCCC AAAGACTGGC GCGCGGCCCA AACCGGCGTG AGCGACGACG AATTCGCGGC GTTCCAGAAG GCGTGGTTCG CCGAGTTGCA CAGCGCCGGC TACGCCGTAC CGCACTGGCC CGCCGAGTGG GGCGGCGGCA TGTCGGTTCC GCAGCAGATC GTGCTGTACT CCGAACTCGC GGCACACGAC GCGCCCCGCC TCGTACTGGC GTTCGTCGGC ATCCACCACG CCGCGTCGAC GCTGCTCGCC GCGGGCACAG ACGCACAGCG CAACCGGCAC CTGCCCGCGA TCCTCGACGG CGAGATCTGG GTACAGGGCT TCTCCGAACC CGAGGCCGGT TCGGATCTGG CGTCGCTGCG CACCACGGCC CGCGCCGAGG GCGACACCTT CGTGGTCAAC GGGCAGAAGC TGTGGGCCAG TGGAGCCATG TACGCCGACT GGTGCCTGCT GCTGGCCCGC ACCGACCCGA ATGCCCCCAA ACGCCACGGC ATTTCCTACT TCATGATGGA CATGAAGACG CCGGGTGTCG ATGTGCGTCC GATCCGCAAC GCGGTCGGCG ATTCGCACTT CTGCGAGATC TTCCTCAACG ACGTCGTGAT CCCGTCGGCC AATCTCGTCG GCCCGGTCAA CAAGGGCTGG CAGGTCGCGC AGGCGACGCT GGGCGCCGAA CGCGGAATGA CCATGCTCGA ACTCGCCGAA CGTCTCGGCA ATGCGGGGTT CCGCTGGCTC GTGGACGCCT GCTCGCCCGT GGATGATCCG GTCGTGGCGG ATCGCCTGGC GCAGTTCGAG ATCGAGCTCA CCGGTTTGCG CGGACTGTGC CGCGACCTGG TCGAACGCAG CGAAACCGGT GAGGCGGGCC CAGCCGACGC CTCGATCGTC AAGCTGTACT ACAGCGAACT GTTGCAACGG ATGACCGATT TCGGCGCCCA GATCGCCGGG CTGCCCGCGC ACACCGTGCT GACCAAACCC GCGTCGAGCG GGTGGGAATC GGGGGCGTGG ATGCTCGACT TCATCGGCTC TTGGGAATGG ACCATCCCCG GCGGGGCCAG CGAGATCCAG CGCACCATCA TCGGTGAGCG GGGTTTGGGC CTGCCGCGAG AGCCGAGTGC GGTGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA