MysmA.01613.c

Putative acyl-CoA dehydrogenase

CENTER ID: MysmA.01613.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01613.c.A1.GE28960 Full length( MysmA.01613.c ) 1 362
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4756
RefSeq: YP_889130.1
UniProt: A0R1U2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFRDSPEEA AFRDRLRSWL ADNAASFKAS GDDYWARQGE WHQALYGAGF FGTSWPKEFG GQDLPPVYDV IVDEELARAG APPRPSLGYL VVGLGHHGSK ELQQRFLPGM INGTERWCQG FSEPGAGSDL ASLITTATRD GDNYIINGHK IWTSYSDVAD WCLLLARTDK DVPRHKGISA FIISMHQDGI QQRPLKMING VTTEFGQVTF DNAVVPASHM VGAPGEGWAL AMTVVSHERE PSTLGYSARY GKLVREMASR VEGRAPQDLA WAAVQAEMLR LHVRRRLSEQ LDGLKHGPQG SLDKLLMTWV EQSVGHAALA VSGTSDPELL SAYLYSRAQS VMGGTSQIQK NIIASRILGL GV
NT Sequence
TTGGATTTTC GTGATTCACC CGAGGAAGCG GCCTTCCGCG ACCGGTTGCG GTCCTGGCTG GCCGACAACG CCGCGTCGTT CAAGGCCTCC GGCGACGACT ACTGGGCCCG TCAGGGGGAG TGGCACCAGG CCCTGTACGG CGCAGGGTTT TTCGGCACGT CGTGGCCCAA GGAGTTCGGC GGGCAGGATT TGCCGCCCGT CTACGACGTG ATCGTCGACG AGGAACTCGC GCGCGCCGGT GCGCCGCCGC GGCCCAGCCT CGGCTATCTC GTCGTCGGCC TGGGACACCA CGGCAGCAAG GAACTGCAGC AGCGGTTCCT GCCCGGCATG ATCAACGGCA CCGAGCGGTG GTGCCAGGGC TTCTCTGAGC CGGGTGCCGG GTCGGACCTG GCGTCGCTGA TCACCACGGC CACGCGCGAC GGCGACAACT ACATCATCAA CGGCCACAAG ATCTGGACCA GCTACTCCGA CGTCGCCGAC TGGTGCCTGC TGCTGGCGCG CACCGACAAG GATGTGCCGC GCCACAAGGG CATCTCGGCG TTCATCATCT CGATGCACCA GGACGGCATC CAGCAGCGTC CGCTCAAGAT GATCAACGGC GTCACCACCG AGTTCGGTCA GGTGACATTC GACAACGCGG TGGTGCCCGC GTCGCACATG GTGGGAGCGC CCGGTGAGGG CTGGGCGCTG GCGATGACCG TCGTCAGCCA CGAACGCGAA CCGTCGACGC TCGGCTACTC CGCACGCTAC GGAAAGCTGG TGCGCGAGAT GGCGTCCCGC GTCGAGGGAC GTGCGCCCCA GGATCTGGCG TGGGCCGCGG TGCAGGCCGA GATGCTGCGC CTGCACGTGC GGCGCCGGTT GTCCGAACAA CTCGACGGGC TCAAGCACGG TCCGCAAGGG TCGCTCGACA AACTGCTCAT GACGTGGGTC GAACAGTCGG TCGGGCACGC CGCGCTCGCG GTCAGCGGGA CCTCGGATCC CGAACTGCTC AGTGCCTACC TGTACAGCCG CGCCCAGAGC GTCATGGGCG GCACCTCGCA GATCCAGAAG AACATCATCG CCTCACGCAT CCTCGGATTG GGAGTT
Details for MysmA.01613.c.A1.GE28960
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 42kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 67
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHXX XXXAQTQGPG SMLDFRDSPE EAAFRDRLRS WLADNAASFK ASGDDYWARQ GEWHQALYGA GFFGTSWPKE FGGQDLPPVY DVIVDEELAR AGAPPRPSLG YLVVGLGHHG SKELQQRFLP GMINGTERWC QGFSEPGAGS DLASLITTAT RDGDNYIING HKIWTSYSDV XDWCLLLART DXDVPRHKGI SAFIISMHQD GXQXRPLKMI XXXTTEFGXX XFXNAXXPXS HMXXAPGXGW XLXXTVXXXX XXPSXLXXSG XXXXAXXXXX XXXXTXXXXX XXXXXXXXXX LXXXXXVPTX XTXXXXXXXX XXXXVLXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
attttgttnn ctttaagaag gaganatncc atggctcatc accatcacca tcatatnnnn nnnnngnnag ctcagaccca gggtcctggt tcgatgttgg attttcgtga ttcacccgag gaagcggcct tccgcgaccg gttgcggtcc tggctggccg acaacgccgc gtcgttcaag gcctccggcg acgactactg ggcccgtcag ggggagtggc accaggccct gtacggcgca gggtttttcg gcacgtcgtg gcccaaggag ttcggcgggc aggatttgcc gcccgtctac gacgtgatcg tcgacgagga actcgcgcgc gccggtgcgc cgccgcggcc cagcctcggc tatctcgtcg tcggcctggg acaccacggc agcaaggaac tgcagcagcg gttcctgccc ggcatgatca acggcaccga gcggtggtgc cagggcttct ctgagccggg tgccgggtcg gacctggcgt cgctgatcac cacggccacg cgcgacggcg acaactacat catcaacggc cacaagatct ggaccagcta ctccgacgtc nccgactggt gcctgctgct ggcgcgcacc gacnaggatg tgccgcgcca caagggcatc tcggcgttca tcatctcgat gcaccaggac ggcntccagc ancgtccgct caagatgatc nacgnnntca ccacngagtt cggncannng annttcnaca acgcgnngnn gcccncgtct cacatgnngn gagcncccgg nganggntgg nnnctggnna ngaccgtcnt cnnncnnnaa nncnaaccgt cnangctcnn ctantccgga cnntnnngnn nagctgnnnc nnnannnncc nncgncnann gnacntgnnn ccnntgnnct nnnnnnnncn ngnnnnncna nnnnntgnan ctgcncnnnn nnannnnngt nccaacnnan nnnacngnnn nagnnnnnnn nncnnnnnnn nntnnnncnn nngtnctagn nnnntnnnna nnnncnnnnn nnnnnnnncn nnntnnnant cnnnanntnn nnnntnnnnn tnncnnnnnn nnncnnana
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDFRDSPE EAAFRDRLRS WLADNAASFK ASGDDYWARQ GEWHQALYGA GFFGTSWPKE FGGQDLPPVY DVIVDEELAR AGAPPRPSLG YLVVGLGHHG SKELQQRFLP GMINGTERWC QGFSEPGAGS DLASLITTAT RDGDNYIING HKIWTSYSDV ADWCLLLART DKDVPRHKGI SAFIISMHQD GIQQRPLKMI NGVTTEFGQV TFDNAVVPAS HMVGAPGEGW ALAMTVVSHE REPSTLGYSA RYGKLVREMA SRVEGRAPQD LAWAAVQAEM LRLHVRRRLS EQLDGLKHGP QGSLDKLLMT WVEQSVGHAA LAVSGTSDPE LLSAYLYSRA QSVMGGTSQI QKNIIASRIL GLGV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggatttt cgtgattcac ccgaggaagc ggccttccgc gaccggttgc ggtcctggct ggccgacaac gccgcgtcgt tcaaggcctc cggcgacgac tactgggccc gtcaggggga gtggcaccag gccctgtacg gcgcagggtt tttcggcacg tcgtggccca aggagttcgg cgggcaggat ttgccgcccg tctacgacgt gatcgtcgac gaggaactcg cgcgcgccgg tgcgccgccg cggcccagcc tcggctatct cgtcgtcggc ctgggacacc acggcagcaa ggaactgcag cagcggttcc tgcccggcat gatcaacggc accgagcggt ggtgccaggg cttctctgag ccgggtgccg ggtcggacct ggcgtcgctg atcaccacgg ccacgcgcga cggcgacaac tacatcatca acggccacaa gatctggacc agctactccg acgtcgccga ctggtgcctg ctgctggcgc gcaccgacaa ggatgtgccg cgccacaagg gcatctcggc gttcatcatc tcgatgcacc aggacggcat ccagcagcgt ccgctcaaga tgatcaacgg cgtcaccacc gagttcggtc aggtgacatt cgacaacgcg gtggtgcccg cgtcgcacat ggtgggagcg cccggtgagg gctgggcgct ggcgatgacc gtcgtcagcc acgaacgcga accgtcgacg ctcggctact ccgcacgcta cggaaagctg gtgcgcgaga tggcgtcccg cgtcgaggga cgtgcgcccc aggatctggc gtgggccgcg gtgcaggccg agatgctgcg cctgcacgtg cggcgccggt tgtccgaaca actcgacggg ctcaagcacg gtccgcaagg gtcgctcgac aaactgctca tgacgtgggt cgaacagtcg gtcgggcacg ccgcgctcgc ggtcagcggg acctcggatc ccgaactgct cagtgcctac ctgtacagcc gcgcccagag cgtcatgggc ggcacctcgc agatccagaa gaacatcatc gcctcacgca tcctcggatt gggagttaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa