MysmA.01613.a

Acyl-CoA dehydrogenase

CENTER ID: MysmA.01613.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01613.a.A1.GE28958 Full length( MysmA.01613.a ) 1 391
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4689
RefSeq: YP_889061.1
UniProt: A0R1M3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLTFDSDVE KFRAEFVAFL DANLPSEAAT LERPRSVSHM PQWARDWQRL LFDNGWLLPA QPPEFGGRNA NVVQQFVHLD ELCRRRIYHS FNPQGVNIIA ASLLTFGTEE QKHRWAVPVL RGEKTASLGM SEPSAGSDLA SLRTRAVRDG DEFVVNGQKV WTSGAHDADW LLTFVRTDPD APKHKGISVL IVPTDTPGVV CRPFADMTGQ ENLDFNEVFF TDARVPAENL VGPLNQGWKV ANGSLGHERT MMWLGFADRI DNMLADFNPR TELERDQYAT TIMDYQALRA LGSAALARAA RGEQDTASVS VLKLLGSEAE RNAFENALAA AGPEGLIHPS TSGPYAHMNL DHYFASWFER YARSFGGTIA GGTSEIQRNI IATQVLGLPR G
NT Sequence
ATGCAGCTGA CATTCGATTC CGACGTCGAG AAGTTCCGCG CGGAGTTCGT GGCGTTCCTC GACGCGAACC TCCCGTCCGA GGCCGCGACG CTGGAGCGGC CCCGGTCGGT CTCGCACATG CCGCAGTGGG CCCGCGACTG GCAGCGCCTG CTGTTCGACA ACGGCTGGCT GCTGCCCGCC CAGCCGCCGG AGTTCGGCGG TCGCAACGCA AACGTCGTGC AGCAGTTCGT CCATCTCGAC GAGCTGTGCC GCCGCCGGAT CTACCACAGC TTCAACCCCC AAGGCGTCAA CATCATCGCG GCGTCGCTCT TGACATTCGG CACCGAGGAG CAGAAGCACC GCTGGGCCGT GCCGGTGCTG CGTGGCGAGA AGACCGCGTC GCTGGGTATG AGTGAGCCGA GCGCGGGTTC GGACCTCGCA TCGCTGCGCA CCCGCGCGGT GCGCGACGGG GATGAGTTCG TGGTCAACGG TCAGAAGGTC TGGACGTCGG GGGCGCACGA CGCCGACTGG CTGCTGACGT TCGTACGCAC CGATCCGGAT GCGCCCAAGC ACAAGGGCAT CAGCGTGCTG ATCGTCCCGA CCGACACCCC CGGCGTGGTG TGCAGGCCGT TCGCGGACAT GACCGGCCAG GAGAACCTCG ACTTCAACGA GGTGTTCTTC ACCGATGCGC GCGTGCCCGC GGAGAACCTC GTGGGCCCGC TGAACCAGGG CTGGAAGGTG GCCAACGGCT CACTGGGCCA CGAGCGCACC ATGATGTGGC TCGGCTTCGC CGACCGCATC GACAACATGC TCGCCGACTT CAACCCCCGC ACCGAACTCG AGCGCGACCA GTACGCCACG ACCATCATGG ACTACCAGGC GTTGCGCGCC TTGGGTTCTG CCGCGCTCGC ACGCGCCGCG CGCGGCGAGC AGGACACCGC GTCGGTGTCG GTGCTCAAAC TGCTGGGGTC CGAGGCCGAG CGCAACGCCT TCGAAAACGC GCTCGCCGCA GCGGGTCCCG AAGGCCTGAT CCATCCGTCG ACCAGCGGGC CCTACGCGCA CATGAACCTC GACCACTACT TCGCGAGCTG GTTCGAGCGC TACGCCAGGA GCTTCGGCGG AACCATCGCA GGCGGCACGT CGGAGATCCA GCGCAACATC ATCGCCACTC AGGTGCTCGG GCTGCCCCGC GGC
Details for MysmA.01613.a.A1.GE28958
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 11
PERCENT COVERAGE: 47
Validated AA Sequence
MVXXXVLXXX XXXSIXXXXX AGXXXXSXXX TSRPRPRRWS GPGRSRTCRX GPATGXXCCS TTAGCCPPSX XSSAVAXXTS CSSSXXSTSC AXAGXTTXST PXRQXHRGVX LDIXXXXXXA XXXXAXAAWX EXVXGYXXXX XXXXXXIXXX XXXXRXXXXX XSXXXGXXXX XXXXXXXXXP XXXXXXXXXX XXXXXXX
Validated NT Sequence
ttttgtttac tttaagaagg anatntnccn tggnncgtcn cnntcnccat ggtcanaann ngggtcctgg ntcnatgnan ntnnnattcn attncgannn nnnnnnccgc gggnnnnnnn ngnngttcct nnnnnngaac ctcccgtccg aggccgcgac gctggagcgg ccccggtcgg tctcgcacat gccgcagngg gcccgcgact ggcancgnct gctgttcgac aacggctggn tgctgcccgc ccagccnnnn gagttcggcg gtcgcancgc anacgtcgtg cagcagttcn ncnntctcga cgagctgtgc cnccgccgga nctaccacan nttcnacccc nnggcgncaa cntcatcgng gngtcnctct tgacattcng cnncnannan nnnaagcnnc nntggnnnnt gcnngngctg cntggnnnga agangtccnt gggtatgann gagncganng cganttnnga nnnnnnatcn ntgnnnnnnn nnncgnnnnn cgncnggnan nantnnntnn ntcnnnngnn nccggnannn nnnnnnnnnn nncnnnnnnn nnnnnntgnn nnnnnccnnn nntnnntnnn nnnnnnnnnn nnngcnntng nnnnnnnnnn nnnnnnnnnn nt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLTFDSDV EKFRAEFVAF LDANLPSEAA TLERPRSVSH MPQWARDWQR LLFDNGWLLP AQPPEFGGRN ANVVQQFVHL DELCRRRIYH SFNPQGVNII AASLLTFGTE EQKHRWAVPV LRGEKTASLG MSEPSAGSDL ASLRTRAVRD GDEFVVNGQK VWTSGAHDAD WLLTFVRTDP DAPKHKGISV LIVPTDTPGV VCRPFADMTG QENLDFNEVF FTDARVPAEN LVGPLNQGWK VANGSLGHER TMMWLGFADR IDNMLADFNP RTELERDQYA TTIMDYQALR ALGSAALARA ARGEQDTASV SVLKLLGSEA ERNAFENALA AAGPEGLIHP STSGPYAHMN LDHYFASWFE RYARSFGGTI AGGTSEIQRN IIATQVLGLP RG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcagctgaca ttcgattccg acgtcgagaa gttccgcgcg gagttcgtgg cgttcctcga cgcgaacctc ccgtccgagg ccgcgacgct ggagcggccc cggtcggtct cgcacatgcc gcagtgggcc cgcgactggc agcgcctgct gttcgacaac ggctggctgc tgcccgccca gccgccggag ttcggcggtc gcaacgcaaa cgtcgtgcag cagttcgtcc atctcgacga gctgtgccgc cgccggatct accacagctt caacccccaa ggcgtcaaca tcatcgcggc gtcgctcttg acattcggca ccgaggagca gaagcaccgc tgggccgtgc cggtgctgcg tggcgagaag accgcgtcgc tgggtatgag tgagccgagc gcgggttcgg acctcgcatc gctgcgcacc cgcgcggtgc gcgacgggga tgagttcgtg gtcaacggtc agaaggtctg gacgtcgggg gcgcacgacg ccgactggct gctgacgttc gtacgcaccg atccggatgc gcccaagcac aagggcatca gcgtgctgat cgtcccgacc gacacccccg gcgtggtgtg caggccgttc gcggacatga ccggccagga gaacctcgac ttcaacgagg tgttcttcac cgatgcgcgc gtgcccgcgg agaacctcgt gggcccgctg aaccagggct ggaaggtggc caacggctca ctgggccacg agcgcaccat gatgtggctc ggcttcgccg accgcatcga caacatgctc gccgacttca acccccgcac cgaactcgag cgcgaccagt acgccacgac catcatggac taccaggcgt tgcgcgcctt gggttctgcc gcgctcgcac gcgccgcgcg cggcgagcag gacaccgcgt cggtgtcggt gctcaaactg ctggggtccg aggccgagcg caacgccttc gaaaacgcgc tcgccgcagc gggtcccgaa ggcctgatcc atccgtcgac cagcgggccc tacgcgcaca tgaacctcga ccactacttc gcgagctggt tcgagcgcta cgccaggagc ttcggcggaa ccatcgcagg cggcacgtcg gagatccagc gcaacatcat cgccactcag gtgctcgggc tgccccgcgg caaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa