MysmA.01608.a

Ribonucleoside-diphosphate reductase, beta subunit

CENTER ID: MysmA.01608.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01608.a.A1.GE28956 Full length( MysmA.01608.a ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01608.a.A1.PS00835 Full length( MysmA.01608.a ) 1 324
MysmA.01608.a.A1.PS00836 Full length( MysmA.01608.a ) 1 324

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2278
RefSeq: YP_885432.1
UniProt: A0QR94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDGIKLIDR VSAINWNRLQ DEKDAEVWHR LTGNFWLPEK VPVSNDIQSW HTLTDNEKQL TMRVFTGLTL LDTIQGTVGA VSLIPDAVTP HEEAVLTNIA FMESVHAKSY SNIFSTLCST AEIDDAFRWS EENPNLQRKA AIVMQYYRGD EPLKRKVAST LLESFLFYSG FYLPMYWSSR AKLTNTADMI RLIIRDEAVH GYYIGYKYQR GLALVDEEKK QELKDYTYEL LFELYDNEVE YTQDLYDSVG LTEDVKKFLR YNANKALMNL GYEALFPRDE TDVNPAILSA LAPNADENHD FFSGSGSSYV IGKAVNTEDE DWDF
NT Sequence
GTGTCCGACG GAATCAAGCT GATCGACCGT GTCTCAGCCA TCAACTGGAA CCGGCTCCAG GATGAGAAGG ACGCCGAGGT GTGGCACCGG CTCACCGGCA ATTTCTGGTT GCCGGAGAAG GTGCCGGTGT CCAACGACAT CCAGTCCTGG CACACCCTCA CCGACAACGA GAAGCAGCTG ACAATGCGGG TGTTCACCGG CCTGACGCTG CTCGACACCA TCCAGGGCAC CGTCGGCGCG GTCAGCCTGA TCCCTGACGC GGTCACCCCG CACGAGGAGG CCGTGCTCAC CAACATCGCG TTCATGGAGT CGGTGCACGC CAAGAGCTAC AGCAACATCT TCTCGACGCT GTGCTCGACC GCCGAGATCG ACGACGCGTT CCGCTGGTCC GAGGAGAACC CGAACCTGCA GCGCAAGGCC GCGATCGTGA TGCAGTACTA CCGCGGCGAC GAGCCGCTCA AGCGCAAGGT GGCCTCGACG CTGCTGGAGA GCTTCCTGTT CTACTCGGGC TTCTACCTGC CGATGTACTG GTCCAGCCGC GCCAAGCTGA CCAACACCGC CGACATGATC CGCCTGATCA TCCGCGACGA GGCCGTGCAC GGCTACTACA TCGGCTACAA GTACCAGCGC GGGCTCGCCC TCGTCGACGA GGAAAAGAAG CAGGAGCTCA AGGACTACAC CTACGAACTG CTCTTCGAGC TCTACGACAA CGAGGTCGAG TACACCCAGG ACCTCTACGA CAGCGTCGGC CTGACCGAGG ACGTCAAGAA GTTCCTGCGC TACAACGCCA ACAAGGCCCT GATGAACCTC GGCTACGAGG CGCTGTTCCC GCGCGACGAG ACCGACGTCA ACCCGGCGAT CCTCTCGGCG CTGGCGCCCA ACGCCGACGA GAACCACGAC TTCTTCTCCG GCTCGGGGTC GAGCTACGTG ATCGGCAAGG CCGTCAACAC CGAAGACGAG GACTGGGACT TC
Details for MysmA.01608.a.A1.GE28956
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 39kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVSDGIKLID RVSAINWNRL QDEKDAEVWH RLTGNFWLPE KVPVSNDIQS WHTLTDNEKQ LTMRVFTGLT LLDTIQGTVG AVSLIPDAVT PHEEAVLTNI AFMESVHAKS YSNIFSTLCS TAEIDDAFRW SEENPNLQRK AAIVMQYYRG DEPLKRKVAS TLLESFLFYS GFYLPMYWSS RAKLTNTADM IRLIIRDEAV HGYYIGYKYQ RGLALVDEEK KQELKDYTYE LLFELYDNEV EYTQDLYDSV GLTEDVKKFL RYNANKALMN LGYEALFPRD ETDVNPAILS ALAPNADENH DFFSGSGSSY VIGKAVNTED EDW
Validated NT Sequence
cccagtcctc gtcttcggtg ttgacggcct tgccgatcac gtagctcgac cccgagccgg agaagaagtc gtggttctcg tcggcgttgg gcgccagcgc cgagaggatc gccgggttga cgtcggtctc gtcgcgcggg aacagcgcct cgtagccgag gttcatcagg gccttgttgg cgttgtagcg caggaacttc ttgacgtcct cggtcaggcc gacgctgtcg tagaggtcct gggtgtactc gacctcgttg tcgtagagct cgaagagcag ttcgtaggtg tagtccttga gctcctgctt cttttcctcg tcgacgaggg cgagcccgcg ctggtacttg tagccgatgt agtagccgtg cacggcctcg tcgcggatga tcaggcggat catgtcggcg gtgttggtca gcttggcgcg gctggaccag tacatcggca ggtagaagcc cgagtagaac aggaagctct ccagcagcgt cgaggccacc ttgcgcttga gcggctcgtc gccgcggtag tactgcatca cgatcgcggc cttgcgctgc aggttcgggt tctcctcgga ccagcggaac gcgtcgtcga tctcggcggt cgagcacagc gtcgagaaga tgttgctgta gctcttggcg tgcaccgact ccatgaacgc gatgttggtg agcacggcct cctcgtgcgg ggtgaccgcg tcagggatca ggctgaccgc gccgacggtg ccctggatgg tgtcgagcag cgtcaggccg gtgaacaccc gcattgtcag ctgcttctcg ttgtcggtga gggtgtgcca ggactggatg tcgttggaca ccggcacctt ctccggcaac cagaaattgc cggtgagccg gtgccacacc tcggcgtcct tctcatcctg gagccggttc cagttgatgg ctgagacacg gtcgatcagc ttgattccgt cggacaccat cgaaccagga ccctgggtct ga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDGIKLI DRVSAINWNR LQDEKDAEVW HRLTGNFWLP EKVPVSNDIQ SWHTLTDNEK QLTMRVFTGL TLLDTIQGTV GAVSLIPDAV TPHEEAVLTN IAFMESVHAK SYSNIFSTLC STAEIDDAFR WSEENPNLQR KAAIVMQYYR GDEPLKRKVA STLLESFLFY SGFYLPMYWS SRAKLTNTAD MIRLIIRDEA VHGYYIGYKY QRGLALVDEE KKQELKDYTY ELLFELYDNE VEYTQDLYDS VGLTEDVKKF LRYNANKALM NLGYEALFPR DETDVNPAIL SALAPNADEN HDFFSGSGSS YVIGKAVNTE DEDWDF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccgac ggaatcaagc tgatcgaccg tgtctcagcc atcaactgga accggctcca ggatgagaag gacgccgagg tgtggcaccg gctcaccggc aatttctggt tgccggagaa ggtgccggtg tccaacgaca tccagtcctg gcacaccctc accgacaacg agaagcagct gacaatgcgg gtgttcaccg gcctgacgct gctcgacacc atccagggca ccgtcggcgc ggtcagcctg atccctgacg cggtcacccc gcacgaggag gccgtgctca ccaacatcgc gttcatggag tcggtgcacg ccaagagcta cagcaacatc ttctcgacgc tgtgctcgac cgccgagatc gacgacgcgt tccgctggtc cgaggagaac ccgaacctgc agcgcaaggc cgcgatcgtg atgcagtact accgcggcga cgagccgctc aagcgcaagg tggcctcgac gctgctggag agcttcctgt tctactcggg cttctacctg ccgatgtact ggtccagccg cgccaagctg accaacaccg ccgacatgat ccgcctgatc atccgcgacg aggccgtgca cggctactac atcggctaca agtaccagcg cgggctcgcc ctcgtcgacg aggaaaagaa gcaggagctc aaggactaca cctacgaact gctcttcgag ctctacgaca acgaggtcga gtacacccag gacctctacg acagcgtcgg cctgaccgag gacgtcaaga agttcctgcg ctacaacgcc aacaaggccc tgatgaacct cggctacgag gcgctgttcc cgcgcgacga gaccgacgtc aacccggcga tcctctcggc gctggcgccc aacgccgacg agaaccacga cttcttctcc ggctcggggt cgagctacgt gatcggcaag gccgtcaaca ccgaagacga ggactgggac ttcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.01608.a.A1.PS00835
PURIFICATION DATe: 11/2/2010
CONCENTRATION: 38.67mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MVSDGIKLID RVSAINWNRL QDEKDAEVWH RLTGNFWLPE KVPVSNDIQS WHTLTDNEKQ LTMRVFTGLT LLDTIQGTVG AVSLIPDAVT PHEEAVLTNI AFMESVHAKS YSNIFSTLCS TAEIDDAFRW SEENPNLQRK AAIVMQYYRG DEPLKRKVAS TLLESFLFYS GFYLPMYWSS RAKLTNTADM IRLIIRDEAV HGYYIGYKYQ RGLALVDEEK KQELKDYTYE LLFELYDNEV EYTQDLYDSV GLTEDVKKFL RYNANKALMN LGYEALFPRD ETDVNPAILS ALAPNADENH DFFSGSGSSY VIGKAVNTED EDW
Validated NT Sequence
cccagtcctc gtcttcggtg ttgacggcct tgccgatcac gtagctcgac cccgagccgg agaagaagtc gtggttctcg tcggcgttgg gcgccagcgc cgagaggatc gccgggttga cgtcggtctc gtcgcgcggg aacagcgcct cgtagccgag gttcatcagg gccttgttgg cgttgtagcg caggaacttc ttgacgtcct cggtcaggcc gacgctgtcg tagaggtcct gggtgtactc gacctcgttg tcgtagagct cgaagagcag ttcgtaggtg tagtccttga gctcctgctt cttttcctcg tcgacgaggg cgagcccgcg ctggtacttg tagccgatgt agtagccgtg cacggcctcg tcgcggatga tcaggcggat catgtcggcg gtgttggtca gcttggcgcg gctggaccag tacatcggca ggtagaagcc cgagtagaac aggaagctct ccagcagcgt cgaggccacc ttgcgcttga gcggctcgtc gccgcggtag tactgcatca cgatcgcggc cttgcgctgc aggttcgggt tctcctcgga ccagcggaac gcgtcgtcga tctcggcggt cgagcacagc gtcgagaaga tgttgctgta gctcttggcg tgcaccgact ccatgaacgc gatgttggtg agcacggcct cctcgtgcgg ggtgaccgcg tcagggatca ggctgaccgc gccgacggtg ccctggatgg tgtcgagcag cgtcaggccg gtgaacaccc gcattgtcag ctgcttctcg ttgtcggtga gggtgtgcca ggactggatg tcgttggaca ccggcacctt ctccggcaac cagaaattgc cggtgagccg gtgccacacc tcggcgtcct tctcatcctg gagccggttc cagttgatgg ctgagacacg gtcgatcagc ttgattccgt cggacaccat cgaaccagga ccctgggtct ga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDGIKLI DRVSAINWNR LQDEKDAEVW HRLTGNFWLP EKVPVSNDIQ SWHTLTDNEK QLTMRVFTGL TLLDTIQGTV GAVSLIPDAV TPHEEAVLTN IAFMESVHAK SYSNIFSTLC STAEIDDAFR WSEENPNLQR KAAIVMQYYR GDEPLKRKVA STLLESFLFY SGFYLPMYWS SRAKLTNTAD MIRLIIRDEA VHGYYIGYKY QRGLALVDEE KKQELKDYTY ELLFELYDNE VEYTQDLYDS VGLTEDVKKF LRYNANKALM NLGYEALFPR DETDVNPAIL SALAPNADEN HDFFSGSGSS YVIGKAVNTE DEDWDF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCCGAC GGAATCAAGC TGATCGACCG TGTCTCAGCC ATCAACTGGA ACCGGCTCCA GGATGAGAAG GACGCCGAGG TGTGGCACCG GCTCACCGGC AATTTCTGGT TGCCGGAGAA GGTGCCGGTG TCCAACGACA TCCAGTCCTG GCACACCCTC ACCGACAACG AGAAGCAGCT GACAATGCGG GTGTTCACCG GCCTGACGCT GCTCGACACC ATCCAGGGCA CCGTCGGCGC GGTCAGCCTG ATCCCTGACG CGGTCACCCC GCACGAGGAG GCCGTGCTCA CCAACATCGC GTTCATGGAG TCGGTGCACG CCAAGAGCTA CAGCAACATC TTCTCGACGC TGTGCTCGAC CGCCGAGATC GACGACGCGT TCCGCTGGTC CGAGGAGAAC CCGAACCTGC AGCGCAAGGC CGCGATCGTG ATGCAGTACT ACCGCGGCGA CGAGCCGCTC AAGCGCAAGG TGGCCTCGAC GCTGCTGGAG AGCTTCCTGT TCTACTCGGG CTTCTACCTG CCGATGTACT GGTCCAGCCG CGCCAAGCTG ACCAACACCG CCGACATGAT CCGCCTGATC ATCCGCGACG AGGCCGTGCA CGGCTACTAC ATCGGCTACA AGTACCAGCG CGGGCTCGCC CTCGTCGACG AGGAAAAGAA GCAGGAGCTC AAGGACTACA CCTACGAACT GCTCTTCGAG CTCTACGACA ACGAGGTCGA GTACACCCAG GACCTCTACG ACAGCGTCGG CCTGACCGAG GACGTCAAGA AGTTCCTGCG CTACAACGCC AACAAGGCCC TGATGAACCT CGGCTACGAG GCGCTGTTCC CGCGCGACGA GACCGACGTC AACCCGGCGA TCCTCTCGGC GCTGGCGCCC AACGCCGACG AGAACCACGA CTTCTTCTCC GGCTCGGGGT CGAGCTACGT GATCGGCAAG GCCGTCAACA CCGAAGACGA GGACTGGGAC TTCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for MysmA.01608.a.A1.PS00836
PURIFICATION DATe: 11/2/2010
CONCENTRATION: 48.31mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MVSDGIKLID RVSAINWNRL QDEKDAEVWH RLTGNFWLPE KVPVSNDIQS WHTLTDNEKQ LTMRVFTGLT LLDTIQGTVG AVSLIPDAVT PHEEAVLTNI AFMESVHAKS YSNIFSTLCS TAEIDDAFRW SEENPNLQRK AAIVMQYYRG DEPLKRKVAS TLLESFLFYS GFYLPMYWSS RAKLTNTADM IRLIIRDEAV HGYYIGYKYQ RGLALVDEEK KQELKDYTYE LLFELYDNEV EYTQDLYDSV GLTEDVKKFL RYNANKALMN LGYEALFPRD ETDVNPAILS ALAPNADENH DFFSGSGSSY VIGKAVNTED EDW
Validated NT Sequence
cccagtcctc gtcttcggtg ttgacggcct tgccgatcac gtagctcgac cccgagccgg agaagaagtc gtggttctcg tcggcgttgg gcgccagcgc cgagaggatc gccgggttga cgtcggtctc gtcgcgcggg aacagcgcct cgtagccgag gttcatcagg gccttgttgg cgttgtagcg caggaacttc ttgacgtcct cggtcaggcc gacgctgtcg tagaggtcct gggtgtactc gacctcgttg tcgtagagct cgaagagcag ttcgtaggtg tagtccttga gctcctgctt cttttcctcg tcgacgaggg cgagcccgcg ctggtacttg tagccgatgt agtagccgtg cacggcctcg tcgcggatga tcaggcggat catgtcggcg gtgttggtca gcttggcgcg gctggaccag tacatcggca ggtagaagcc cgagtagaac aggaagctct ccagcagcgt cgaggccacc ttgcgcttga gcggctcgtc gccgcggtag tactgcatca cgatcgcggc cttgcgctgc aggttcgggt tctcctcgga ccagcggaac gcgtcgtcga tctcggcggt cgagcacagc gtcgagaaga tgttgctgta gctcttggcg tgcaccgact ccatgaacgc gatgttggtg agcacggcct cctcgtgcgg ggtgaccgcg tcagggatca ggctgaccgc gccgacggtg ccctggatgg tgtcgagcag cgtcaggccg gtgaacaccc gcattgtcag ctgcttctcg ttgtcggtga gggtgtgcca ggactggatg tcgttggaca ccggcacctt ctccggcaac cagaaattgc cggtgagccg gtgccacacc tcggcgtcct tctcatcctg gagccggttc cagttgatgg ctgagacacg gtcgatcagc ttgattccgt cggacaccat cgaaccagga ccctgggtct ga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDGIKLI DRVSAINWNR LQDEKDAEVW HRLTGNFWLP EKVPVSNDIQ SWHTLTDNEK QLTMRVFTGL TLLDTIQGTV GAVSLIPDAV TPHEEAVLTN IAFMESVHAK SYSNIFSTLC STAEIDDAFR WSEENPNLQR KAAIVMQYYR GDEPLKRKVA STLLESFLFY SGFYLPMYWS SRAKLTNTAD MIRLIIRDEA VHGYYIGYKY QRGLALVDEE KKQELKDYTY ELLFELYDNE VEYTQDLYDS VGLTEDVKKF LRYNANKALM NLGYEALFPR DETDVNPAIL SALAPNADEN HDFFSGSGSS YVIGKAVNTE DEDWDF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGTCCGAC GGAATCAAGC TGATCGACCG TGTCTCAGCC ATCAACTGGA ACCGGCTCCA GGATGAGAAG GACGCCGAGG TGTGGCACCG GCTCACCGGC AATTTCTGGT TGCCGGAGAA GGTGCCGGTG TCCAACGACA TCCAGTCCTG GCACACCCTC ACCGACAACG AGAAGCAGCT GACAATGCGG GTGTTCACCG GCCTGACGCT GCTCGACACC ATCCAGGGCA CCGTCGGCGC GGTCAGCCTG ATCCCTGACG CGGTCACCCC GCACGAGGAG GCCGTGCTCA CCAACATCGC GTTCATGGAG TCGGTGCACG CCAAGAGCTA CAGCAACATC TTCTCGACGC TGTGCTCGAC CGCCGAGATC GACGACGCGT TCCGCTGGTC CGAGGAGAAC CCGAACCTGC AGCGCAAGGC CGCGATCGTG ATGCAGTACT ACCGCGGCGA CGAGCCGCTC AAGCGCAAGG TGGCCTCGAC GCTGCTGGAG AGCTTCCTGT TCTACTCGGG CTTCTACCTG CCGATGTACT GGTCCAGCCG CGCCAAGCTG ACCAACACCG CCGACATGAT CCGCCTGATC ATCCGCGACG AGGCCGTGCA CGGCTACTAC ATCGGCTACA AGTACCAGCG CGGGCTCGCC CTCGTCGACG AGGAAAAGAA GCAGGAGCTC AAGGACTACA CCTACGAACT GCTCTTCGAG CTCTACGACA ACGAGGTCGA GTACACCCAG GACCTCTACG ACAGCGTCGG CCTGACCGAG GACGTCAAGA AGTTCCTGCG CTACAACGCC AACAAGGCCC TGATGAACCT CGGCTACGAG GCGCTGTTCC CGCGCGACGA GACCGACGTC AACCCGGCGA TCCTCTCGGC GCTGGCGCCC AACGCCGACG AGAACCACGA CTTCTTCTCC GGCTCGGGGT CGAGCTACGT GATCGGCAAG GCCGTCAACA CCGAAGACGA GGACTGGGAC TTCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA