MysmA.01566.c

Enoyl-CoA hydratase/isomerase family protein

CENTER ID: MysmA.01566.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01566.c.A1.GE28951 Full length( MysmA.01566.c ) 1 260
MysmA.01566.c.AE1.GE43167 Full length( MysmA.01566.c ) 1 260
MysmA.01566.c.B1.GE43089 Full length( MysmA.01566.c ) 1 260
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2186
RefSeq: YP_886570.1
UniProt: A0QUI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEYETVLLD LDHEVRVATV TLNRPESLNS FNRTMCEEMR QIWHVIKGDE QINAVVLRAA GDRAFSAGLD VKSRYGQPEI VWNHEDPGEL LSPKWQKMWK PVVCAVHGMC TAGALYFVNE SDVVICSSDA TFFDSHVSAG LVSALEPVGL MRRVGLGDTL RMALMGNDER VSADTALRIG LVTEVVPREQ LWARAHQIAS TIATKPPTAT QGTVRAIWES LDRPYRAAMD QGLIYTRLGN PIAKAELADR PVPKTPPRIR
NT Sequence
ATGACCGAAT ACGAGACGGT GCTGCTCGAC CTGGACCACG AGGTCCGGGT CGCCACCGTG ACGCTGAACC GGCCCGAGAG CCTGAACTCG TTCAACCGCA CCATGTGCGA GGAGATGCGG CAGATCTGGC ACGTGATCAA GGGGGACGAG CAGATCAACG CGGTGGTGCT GCGCGCCGCG GGCGACCGTG CGTTCAGCGC GGGCCTCGAC GTCAAGTCCA GGTACGGTCA GCCCGAGATC GTGTGGAACC ACGAGGATCC CGGTGAGCTG CTCAGCCCCA AATGGCAGAA GATGTGGAAG CCCGTGGTGT GTGCGGTGCA CGGCATGTGC ACGGCGGGCG CGCTGTACTT CGTCAACGAG TCCGACGTCG TGATCTGCTC GTCGGACGCC ACGTTCTTCG ACTCCCATGT CAGCGCGGGA CTGGTGTCCG CGCTCGAGCC GGTCGGGTTG ATGCGGCGCG TCGGCCTCGG TGACACGTTG CGAATGGCGT TGATGGGCAA CGACGAACGC GTGAGCGCGG ACACGGCGCT GCGTATCGGT CTGGTGACCG AGGTGGTGCC CCGCGAACAA TTGTGGGCGC GTGCGCACCA GATCGCGTCG ACCATCGCGA CCAAACCCCC GACCGCCACA CAGGGCACGG TGAGGGCGAT CTGGGAATCG CTGGACCGGC CCTACCGGGC CGCGATGGAC CAGGGCCTGA TCTACACGCG CCTGGGCAAC CCGATCGCGA AGGCCGAACT GGCCGACCGT CCCGTCCCCA AGACACCTCC GCGGATCAGG
Details for MysmA.01566.c.A1.GE28951
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 92
Validated AA Sequence
MTXYETVLLD LDHEVRVATV TLNRPESLNS FNRTMCEEMR QIWHVIKGDE QINAVVLRAA GDRAFSAGLD VKSRYGQPEI VWNHEDPGEL LSPKWQKMWK PVVCAVHGMC TAGALYFVNE SDVVICSSDA TFFDSHVSAG LVSALEPVGL MRRVGLGDTL RMALMGNDER VSADTALRIG LVTEVVPREQ LWARAHQIAS TIATKPPTAT QGTVRAIWES LDRPYRAAMD QGLIYTRLGN PIAKAELADR PVPKTPPRIR
Validated NT Sequence
tattacctga tccgcggagg tgtcttgggg acgggacggt cggccagttc ggccttcgcg atcgggttgc ccaggcgcgt gtagatcagg ccctggtcca tcgcggcccg gtagggccgg tccagcgatt cccagatcgc cctcaccgtg ccctgtgtgg cggtcggggg tttggtcgcg atggtcgacg cgatctggtg cgcacgcgcc cacaattgtt cgcggggcac cacctcggtc accagaccga tacgcagcgc cgtgtccgcg ctcacgcgtt cgtcgttgcc catcaacgcc attcgcaacg tgtcaccgag gccgacgcgc cgcatcaacc cgaccggctc gagcgcggac accagtcccg cgctgacatg ggagtcgaag aacgtggcgt ccgacgagca gatcacgacg tcggactcgt tgacgaagta cagcgcgccc gccgtgcaca tgccgtgcac cgcacacacc acgggcttcc acatcttctg ccatttgggg ctgagcagct caccgggatc ctcgtggttc cacacgatct cgggctgacc gtacctggac ttgacgtcga ggcccgcgct gaacgcacgg tcgcccgcgg cgcgcagcac caccgcgttg atctgctcgt cccccttgat cacgtgccag atctgccgca tctcctcgca catggtgcgg ttgaacgagt tcaggctctc gggccggttc agcgtcacgg tggcgacccg gacctcgtgg tccaggtcga gcagcaccgt ctcgtantcg gtcatcgaac caggaccctg ggtctga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEYETVLL DLDHEVRVAT VTLNRPESLN SFNRTMCEEM RQIWHVIKGD EQINAVVLRA AGDRAFSAGL DVKSRYGQPE IVWNHEDPGE LLSPKWQKMW KPVVCAVHGM CTAGALYFVN ESDVVICSSD ATFFDSHVSA GLVSALEPVG LMRRVGLGDT LRMALMGNDE RVSADTALRI GLVTEVVPRE QLWARAHQIA STIATKPPTA TQGTVRAIWE SLDRPYRAAM DQGLIYTRLG NPIAKAELAD RPVPKTPPRI R
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaatac gagacggtgc tgctcgacct ggaccacgag gtccgggtcg ccaccgtgac gctgaaccgg cccgagagcc tgaactcgtt caaccgcacc atgtgcgagg agatgcggca gatctggcac gtgatcaagg gggacgagca gatcaacgcg gtggtgctgc gcgccgcggg cgaccgtgcg ttcagcgcgg gcctcgacgt caagtccagg tacggtcagc ccgagatcgt gtggaaccac gaggatcccg gtgagctgct cagccccaaa tggcagaaga tgtggaagcc cgtggtgtgt gcggtgcacg gcatgtgcac ggcgggcgcg ctgtacttcg tcaacgagtc cgacgtcgtg atctgctcgt cggacgccac gttcttcgac tcccatgtca gcgcgggact ggtgtccgcg ctcgagccgg tcgggttgat gcggcgcgtc ggcctcggtg acacgttgcg aatggcgttg atgggcaacg acgaacgcgt gagcgcggac acggcgctgc gtatcggtct ggtgaccgag gtggtgcccc gcgaacaatt gtgggcgcgt gcgcaccaga tcgcgtcgac catcgcgacc aaacccccga ccgccacaca gggcacggtg agggcgatct gggaatcgct ggaccggccc taccgggccg cgatggacca gggcctgatc tacacgcgcc tgggcaaccc gatcgcgaag gccgaactgg ccgaccgtcc cgtccccaag acacctccgc ggatcaggaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for MysmA.01566.c.AE1.GE43167
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 24
PERCENT COVERAGE: 101
Validated AA Sequence
MMMMVVPSPD PRRCLGDGTV GQFGLRDRVA QARVDQALVH RGPVGPVQRF PDRPHRALCG GRGFGRDGRR DLVRTRPQLF AGHHLGHQTD TQRRVRAHAF VVAHQRHSQR VTEADAPHQP DRLERGHQSR ADMGVEERGV RRADHDVGLV DEVQRARRAH AVHRTHHGLP HLLPFGAEQL TGILVVPHDL GLTVPGLDVE ARAERTVARG AQHHRVDLLV PLDHVPDLPH LLAHGAVERV QALGPVQRHG GDPDLVVQVE QHRLVFGHGR
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ccctgatccg cggaggtgtc ttggggacgg gacggtcggc cagttcggcc ttcgcgatcg ggttgcccag gcgcgtgtag atcaggccct ggtccatcgc ggcccggtag ggccggtcca gcgattccca gatcgccctc accgtgccct gtgtggcggt cgggggtttg gtcgcgatgg tcgacgcgat ctggtgcgca cgcgcccaca attgttcgcg gggcaccacc tcggtcacca gaccgatacg cagcgccgtg tccgcgctca cgcgttcgtc gttgcccatc aacgccattc gcaacgtgtc accgaggccg acgcgccgca tcaacccgac cggctcgagc gcggacacca gtcccgcgct gacatgggag tcgaagaacg tggcgtccga cgagcagatc acgacgtcgg actcgttgac gaagtacagc gcgcccgccg tgcacatgcc gtgcaccgca cacaccacgg gcttccacat cttctgccat ttggggctga gcagctcacc gggatcctcg tggttccaca cgatctcggg ctgaccgtac ctggacttga cgtcgaggcc cgcgctgaac gcacggtcgc ccgcggcgcg cagcaccacc gcgttgatct gctcgtcccc cttgatcacg tgccagatct gccgcatctc ctcgcacatg gtgcggttga acgagttcag gctctcgggc cggttcagcg tcacggtggc gacccggacc tcgtggtcca ggtcgagcag caccgtctcg tattcggtca tgggagaga
Expected Protein Sequence
MTEYETVLLD LDHEVRVATV TLNRPESLNS FNRTMCEEMR QIWHVIKGDE QINAVVLRAA GDRAFSAGLD VKSRYGQPEI VWNHEDPGEL LSPKWQKMWK PVVCAVHGMC TAGALYFVNE SDVVICSSDA TFFDSHVSAG LVSALEPVGL MRRVGLGDTL RMALMGNDER VSADTALRIG LVTEVVPREQ LWARAHQIAS TIATKPPTAT QGTVRAIWES LDRPYRAAMD QGLIYTRLGN PIAKAELADR PVPKTPPRIR GHHHHHH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaccgaatac gagacggtgc tgctcgacct ggaccacgag gtccgggtcg ccaccgtgac gctgaaccgg cccgagagcc tgaactcgtt caaccgcacc atgtgcgagg agatgcggca gatctggcac gtgatcaagg gggacgagca gatcaacgcg gtggtgctgc gcgccgcggg cgaccgtgcg ttcagcgcgg gcctcgacgt caagtccagg tacggtcagc ccgagatcgt gtggaaccac gaggatcccg gtgagctgct cagccccaaa tggcagaaga tgtggaagcc cgtggtgtgt gcggtgcacg gcatgtgcac ggcgggcgcg ctgtacttcg tcaacgagtc cgacgtcgtg atctgctcgt cggacgccac gttcttcgac tcccatgtca gcgcgggact ggtgtccgcg ctcgagccgg tcgggttgat gcggcgcgtc ggcctcggtg acacgttgcg aatggcgttg atgggcaacg acgaacgcgt gagcgcggac acggcgctgc gtatcggtct ggtgaccgag gtggtgcccc gcgaacaatt gtgggcgcgt gcgcaccaga tcgcgtcgac catcgcgacc aaacccccga ccgccacaca gggcacggtg agggcgatct gggaatcgct ggaccggccc taccgggccg cgatggacca gggcctgatc tacacgcgcc tgggcaaccc gatcgcgaag gccgaactgg ccgaccgtcc cgtccccaag acacctccgc ggatcagggg gcaccaccat catcatcatt aacggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggat
Details for MysmA.01566.c.B1.GE43089
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 60
Validated AA Sequence
MWKPVVCAVH GMCTAGALYF VNESDVVICS SDATFFDSHV SAGLVSALEP VGLMRRVGLG DTLRMALMGN DERVSADTAL RIGLVTEVVP REQLWARAHQ IASTIATKPP TATQGTVRAI WESLDRPYRA AMDQGLIYTR LGNPIAKAEL ADRPVXXXPP RIR
Validated NT Sequence
atcttactca cttacctgat ccgcggaggt ntnnnnnnna cgggacggtc ggccagttcg gccttcgcga tcgggttgcc caggcgcgtg tagatcaggc cctggtccat cgcggcccgg tagggccggt ccagcgattc ccagatcgcc ctcaccgtgc cctgtgtggc ggtcgggggt ttggtcgcga tggtcgacgc gatctggtgc gcacgcgccc acaattgttc gcggggcacc acctcggtca ccagaccgat acgcagcgcc gtgtccgcgc tcacgcgttc gtcgttgccc atcaacgcca ttcgcaacgt gtcaccgagg ccgacgcgcc gcatcaaccc gaccggctcg agcgcggaca ccagtcccgc gctgacatgg gagtcgaaga acgtggcgtc cgacgagcag atcacgacgt cggactcgtt gacgaagtac agcgcgcccg ccgtgcacat gccgtgcacc gcacacacca cgggcttcca catcttctgc catttggggc tgagcagctc accgggatcc tcgtggttcc acacgatctc gggctgaccg tacctggact tgacgtcgag gcccgcgctg aacgcacggt cgcccgcggc gccgcagcac caccgcgttg atctgctcgt cccccttgat cacgtgccag atctgccgca tctcctnnca catggtgcgg ttgaacnnnn tnaggctctc nnncnnnnnn nnngtcacgg tggnnnnccg gacctcgtgg tcnnnnnnnn gcagcaccgt ctcgtattnn gtcnnnnnnn ng
Expected Protein Sequence
MAHHHHHHMT EYETVLLDLD HEVRVATVTL NRPESLNSFN RTMCEEMRQI WHVIKGDEQI NAVVLRAAGD RAFSAGLDVK SRYGQPEIVW NHEDPGELLS PKWQKMWKPV VCAVHGMCTA GALYFVNESD VVICSSDATF FDSHVSAGLV SALEPVGLMR RVGLGDTLRM ALMGNDERVS ADTALRIGLV TEVVPREQLW ARAHQIASTI ATKPPTATQG TVRAIWESLD RPYRAAMDQG LIYTRLGNPI AKAELADRPV PKTPPRIR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg aatacgagac ggtgctgctc gacctggacc acgaggtccg ggtcgccacc gtgacgctga accggcccga gagcctgaac tcgttcaacc gcaccatgtg cgaggagatg cggcagatct ggcacgtgat caagggggac gagcagatca acgcggtggt gctgcgcgcc gcgggcgacc gtgcgttcag cgcgggcctc gacgtcaagt ccaggtacgg tcagcccgag atcgtgtgga accacgagga tcccggtgag ctgctcagcc ccaaatggca gaagatgtgg aagcccgtgg tgtgtgcggt gcacggcatg tgcacggcgg gcgcgctgta cttcgtcaac gagtccgacg tcgtgatctg ctcgtcggac gccacgttct tcgactccca tgtcagcgcg ggactggtgt ccgcgctcga gccggtcggg ttgatgcggc gcgtcggcct cggtgacacg ttgcgaatgg cgttgatggg caacgacgaa cgcgtgagcg cggacacggc gctgcgtatc ggtctggtga ccgaggtggt gccccgcgaa caattgtggg cgcgtgcgca ccagatcgcg tcgaccatcg cgaccaaacc cccgaccgcc acacagggca cggtgagggc gatctgggaa tcgctggacc ggccctaccg ggccgcgatg gaccagggcc tgatctacac gcgcctgggc aacccgatcg cgaaggccga actggccgac cgtcccgtcc ccaagacacc tccgcggatc aggtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat