MysmA.01566.b

Probable enoyl-CoA hydratase

CENTER ID: MysmA.01566.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01566.b.A1.GE28950 Full length( MysmA.01566.b ) 1 255
MysmA.01566.b.AE1.GE43166 Full length( MysmA.01566.b ) 1 255
MysmA.01566.b.B1.GE43088 Full length( MysmA.01566.b ) 1 255
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01566.b.A1.PS00700 Full length( MysmA.01566.b ) 1 255

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.2024
RefSeq: YP_886409.1
UniProt: A0QU21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIVLTADHG AVRVLTMNRP EARNALGSEL IEALFTALQD ADDDESVRAV VLTGADPAFC AGVDLKQAQT LGMDYFARFQ THNCIAKTGQ MATPIIGAIN GATFTGGLEM ALGCDFLIAS ERAVFADTHA RVGILPGGGM TARLPQVVGA AMARRLSMTG EVVDASRAER LGLVTEVVAH EQLLVRALEL AQQIAEVPGP IMSGLKEIYT LGADPVITPA LDAEQQIAGT QQRDFAGLGD RYRAVAERNH AQIQR
NT Sequence
ATGTCGATCG TGCTCACCGC CGACCACGGT GCCGTCCGCG TGCTCACCAT GAACCGTCCC GAGGCCCGCA ACGCGCTGGG CAGCGAGCTG ATCGAGGCGC TGTTCACCGC GCTGCAGGAC GCCGATGACG ACGAATCTGT GCGGGCCGTG GTGTTGACCG GTGCGGACCC GGCGTTCTGC GCGGGTGTCG ACCTCAAGCA GGCGCAGACG CTCGGTATGG ACTATTTCGC GCGGTTCCAG ACGCACAACT GCATCGCCAA GACCGGGCAG ATGGCGACCC CGATCATCGG CGCGATCAAC GGCGCGACGT TCACCGGCGG CCTGGAGATG GCACTGGGCT GCGACTTCCT GATCGCCTCC GAACGTGCGG TGTTCGCCGA TACCCATGCG CGCGTGGGGA TTCTGCCCGG CGGCGGAATG ACGGCGCGAC TGCCGCAGGT GGTCGGCGCC GCAATGGCGC GCCGGTTGTC GATGACCGGT GAGGTGGTCG ACGCCTCACG CGCCGAACGC CTGGGATTGG TCACCGAGGT GGTGGCGCAC GAGCAGCTCC TGGTCCGCGC ACTCGAACTC GCCCAGCAGA TCGCCGAGGT ACCGGGCCCG ATCATGTCCG GCCTCAAAGA GATCTACACA CTTGGCGCTG ATCCGGTCAT CACTCCCGCG CTGGACGCCG AGCAGCAGAT CGCCGGAACG CAGCAGCGCG ACTTCGCCGG TCTCGGCGAC AGATACCGTG CGGTCGCCGA ACGCAACCAC GCCCAGATCC AGCGC
Details for MysmA.01566.b.A1.GE28950
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 29kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIVLTADH GAVRVLTMNR PEARNALGSE LIEALFTALQ DADDDESVRA VVLTGADPAF CAGVDLKQAQ TLGMDYFARF QTHNCIAKTG QMATPIIGAI NGATFTGGLE MALGCDFLIA SERAVFADTH ARVGILPGGG MTARLPQVVG AAMARRLSMT GEVVDASRAE RLGLVTEVVA HEQLLVRALE LAQQIAEVPG PIMSGLKEIY TLGADPVITP ALDAEQQIAG TQQRDFAGLG DRYRAVAERN HAQ
Validated NT Sequence
atctgggcgt ggttgcgttc ggcgaccgca cggtatctgt cgccgagacc ggcgaagtcg cgctgctgcg ttccggcgat ctgctgctcg gcgtccagcg cgggagtgat gaccggatca gcgccaagtg tgtagatctc tttgaggccg gacatgatcg ggcccggtac ctcggcgatc tgctgggcga gttcgagtgc gcggaccagg agctgctcgt gcgccaccac ctcggtgacc aatcccaggc gttcggcgcg tgaggcgtcg accacctcac cggtcatcga caaccggcgc gccattgcgg cgccgaccac ctgcggcagt cgcgccgtca ttccgccgcc gggcagaatc cccacgcgcg catgggtatc ggcgaacacc gcacgttcgg aggcgatcag gaagtcgcag cccagtgcca tctccaggcc gccggtgaac gtcgcgccgt tgatcgcgcc gatgatcggg gtcgccatct gcccggtctt ggcgatgcag ttgtgcgtct ggaaccgcgc gaaatagtcc ataccgagcg tctgcgcctg cttgaggtcg acacccgcgc agaacgccgg gtccgcaccg gtcaacacca cggcccgcac agattcgtcg tcatcggcgt cctgcagcgc ggtgaacagc gcctcgatca gctcgctgcc cagcgcgttg cgggcctcgg gacggttcat ggtgagcacg cggacggcac cgtggtcggc ggtgagcacg atcgacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIVLTADH GAVRVLTMNR PEARNALGSE LIEALFTALQ DADDDESVRA VVLTGADPAF CAGVDLKQAQ TLGMDYFARF QTHNCIAKTG QMATPIIGAI NGATFTGGLE MALGCDFLIA SERAVFADTH ARVGILPGGG MTARLPQVVG AAMARRLSMT GEVVDASRAE RLGLVTEVVA HEQLLVRALE LAQQIAEVPG PIMSGLKEIY TLGADPVITP ALDAEQQIAG TQQRDFAGLG DRYRAVAERN HAQIQR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgatcgtg ctcaccgccg accacggtgc cgtccgcgtg ctcaccatga accgtcccga ggcccgcaac gcgctgggca gcgagctgat cgaggcgctg ttcaccgcgc tgcaggacgc cgatgacgac gaatctgtgc gggccgtggt gttgaccggt gcggacccgg cgttctgcgc gggtgtcgac ctcaagcagg cgcagacgct cggtatggac tatttcgcgc ggttccagac gcacaactgc atcgccaaga ccgggcagat ggcgaccccg atcatcggcg cgatcaacgg cgcgacgttc accggcggcc tggagatggc actgggctgc gacttcctga tcgcctccga acgtgcggtg ttcgccgata cccatgcgcg cgtggggatt ctgcccggcg gcggaatgac ggcgcgactg ccgcaggtgg tcggcgccgc aatggcgcgc cggttgtcga tgaccggtga ggtggtcgac gcctcacgcg ccgaacgcct gggattggtc accgaggtgg tggcgcacga gcagctcctg gtccgcgcac tcgaactcgc ccagcagatc gccgaggtac cgggcccgat catgtccggc ctcaaagaga tctacacact tggcgctgat ccggtcatca ctcccgcgct ggacgccgag cagcagatcg ccggaacgca gcagcgcgac ttcgccggtc tcggcgacag ataccgtgcg gtcgccgaac gcaaccacgc ccagatccag cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.01566.b.AE1.GE43166
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MSIVLTADHG AVRVLTMNRP EARNALGSEL IEALFTALQD ADDDESVRAV VLTGADPAFC AGVDLKQAQT LGMDYFARFQ THNCIAKTGQ MATPIIGAIN GATFTGGLEM ALGCDFLIAS ERAVFADTHA RVGILPGGGM TARLPQVVGA AMARRLSMTG EVVDASRAER LGLVTEVVAH EQLLVRALEL AQQIAEVPGP IMSGLKEIYT LGADPVITPA LDAEQQIAGT QQRDFAGLGD RYRAVAERNH AQIQRAXHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgccnng cgcgctggat ctgggcgtgg ttgcgttcgg cgaccgcacg gtatctgtcg ccgagaccgg cgaagtcgcg ctgctgcgtt ccggcgatct gctgctcggc gtccagcgcg ggagtgatga ccggatcagc gccaagtgtg tagatctctt tgaggccgga catgatcggg cccggtacct cggcgatctg ctgggcgagt tcgagtgcgc ggaccaggag ctgctcgtgc gccaccacct cggtgaccaa tcccaggcgt tcggcgcgtg aggcgtcgac cacctcaccg gtcatcgaca accggcgcgc cattgcggcg ccgaccacct gcggcagtcg cgccgtcatt ccgccgccgg gcagaatccc cacgcgcgca tgggtatcgg cgaacaccgc acgttcggag gcgatcagga agtcgcagcc cagtgccatc tccaggccgc cggtgaacgt cgcgccgttg atcgcgccga tgatcggggt cgccatctgc ccggtcttgg cgatgcagtt gtgcgtctgg aaccgcgcga aatagtccat accgagcgtc tgcgcctgct tgaggtcgac acccgcgcag aacgccgggt ccgcaccggt caacaccacg gcccgcacag attcgtcgtc atcggcgtcc tgcagcgcgg tgaacagcgc ctcgatcagc tcgctgccca gcgcgttgcg ggcctcggga cggttcatgg tgagcacgcg gacggcaccg tggtcggcgg tgagcacgat cgacatggga gaga
Expected Protein Sequence
MSIVLTADHG AVRVLTMNRP EARNALGSEL IEALFTALQD ADDDESVRAV VLTGADPAFC AGVDLKQAQT LGMDYFARFQ THNCIAKTGQ MATPIIGAIN GATFTGGLEM ALGCDFLIAS ERAVFADTHA RVGILPGGGM TARLPQVVGA AMARRLSMTG EVVDASRAER LGLVTEVVAH EQLLVRALEL AQQIAEVPGP IMSGLKEIYT LGADPVITPA LDAEQQIAGT QQRDFAGLGD RYRAVAERNH AQIQRGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gtcgatcgtg ctcaccgccg accacggtgc cgtccgcgtg ctcaccatga accgtcccga ggcccgcaac gcgctgggca gcgagctgat cgaggcgctg ttcaccgcgc tgcaggacgc cgatgacgac gaatctgtgc gggccgtggt gttgaccggt gcggacccgg cgttctgcgc gggtgtcgac ctcaagcagg cgcagacgct cggtatggac tatttcgcgc ggttccagac gcacaactgc atcgccaaga ccgggcagat ggcgaccccg atcatcggcg cgatcaacgg cgcgacgttc accggcggcc tggagatggc actgggctgc gacttcctga tcgcctccga acgtgcggtg ttcgccgata cccatgcgcg cgtggggatt ctgcccggcg gcggaatgac ggcgcgactg ccgcaggtgg tcggcgccgc aatggcgcgc cggttgtcga tgaccggtga ggtggtcgac gcctcacgcg ccgaacgcct gggattggtc accgaggtgg tggcgcacga gcagctcctg gtccgcgcac tcgaactcgc ccagcagatc gccgaggtac cgggcccgat catgtccggc ctcaaagaga tctacacact tggcgctgat ccggtcatca ctcccgcgct ggacgccgag cagcagatcg ccggaacgca gcagcgcgac ttcgccggtc tcggcgacag ataccgtgcg gtcgccgaac gcaaccacgc ccagatccag cgcgggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MysmA.01566.b.B1.GE43088
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHXX IVLTADHGAV RVLTMNRPEA RNALGSELIE ALFTALQDAD DDESVRAVVL TGADPAFCAG VDLKQAQTLG MDYFARFQTH NCIAKTGQMA TPIIGAINGA TFTGGLEMAL GCDFLIASER AVFADTHARV GILPGGGMTA RLPQVVGAAM ARRLSMTGEV VDASRAERLG LVTEVVAHEQ LLVRALELAQ QIAEVPGPIM SGLKEIYTLG ADPVITPALD AEQQIAGTQQ RDFAGLGDRY RAVAXXXXAQ IQR
Validated NT Sequence
atcttactca cttagcgctg gatctgggcn nnnnnnnnnn nggcgaccgc acggtatctg tcgccgagac cggcgaagtc gcgctgctgc gttccggcga tctgctgctc ggcgtccagc gcgggagtga tgaccggatc agcgccaagt gtgtagatct ctttgaggcc ggacatgatc gggcccggta cctcggcgat ctgctgggcg agttcgagtg cgcggaccag gagctgctcg tgcgccacca cctcggtgac caatcccagg cgttcggcgc gtgaggcgtc gaccacctca ccggtcatcg acaaccggcg cgccattgcg gcgccgacca cctgcggcag tcgcgccgtc attccgccgc cgggcagaat ccccacgcgc gcatgggtat cggcgaacac cgcacgttcg gaggcgatca ggaagtcgca gcccagtgcc atctccaggc cgccggtgaa cgtcgcgccg ttgatcgcgc cgatgatcgg ggtcgccatc tgcccggtct tggcgatgca gttgtgcgtc tggaaccgcg cgaaatagtc cataccgagc gtctgcgcct gcttgaggtc gacacccgcg cagaacgccg ggtccgcacc ggtcaacacc acggcccgca cagattcgtc gtcatcggcg tcctgcagcg cggtgaacag cgcctcgatc agctcgctgc ccagcgcgtt gcgggcctcg ggacggttca tggtgagcac gcggacggca ccgtggtcgg cggtgagcac gatcnnnata tggtggtggt ggtggtgagc catggtnt
Expected Protein Sequence
MAHHHHHHMS IVLTADHGAV RVLTMNRPEA RNALGSELIE ALFTALQDAD DDESVRAVVL TGADPAFCAG VDLKQAQTLG MDYFARFQTH NCIAKTGQMA TPIIGAINGA TFTGGLEMAL GCDFLIASER AVFADTHARV GILPGGGMTA RLPQVVGAAM ARRLSMTGEV VDASRAERLG LVTEVVAHEQ LLVRALELAQ QIAEVPGPIM SGLKEIYTLG ADPVITPALD AEQQIAGTQQ RDFAGLGDRY RAVAERNHAQ IQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga tcgtgctcac cgccgaccac ggtgccgtcc gcgtgctcac catgaaccgt cccgaggccc gcaacgcgct gggcagcgag ctgatcgagg cgctgttcac cgcgctgcag gacgccgatg acgacgaatc tgtgcgggcc gtggtgttga ccggtgcgga cccggcgttc tgcgcgggtg tcgacctcaa gcaggcgcag acgctcggta tggactattt cgcgcggttc cagacgcaca actgcatcgc caagaccggg cagatggcga ccccgatcat cggcgcgatc aacggcgcga cgttcaccgg cggcctggag atggcactgg gctgcgactt cctgatcgcc tccgaacgtg cggtgttcgc cgatacccat gcgcgcgtgg ggattctgcc cggcggcgga atgacggcgc gactgccgca ggtggtcggc gccgcaatgg cgcgccggtt gtcgatgacc ggtgaggtgg tcgacgcctc acgcgccgaa cgcctgggat tggtcaccga ggtggtggcg cacgagcagc tcctggtccg cgcactcgaa ctcgcccagc agatcgccga ggtaccgggc ccgatcatgt ccggcctcaa agagatctac acacttggcg ctgatccggt catcactccc gcgctggacg ccgagcagca gatcgccgga acgcagcagc gcgacttcgc cggtctcggc gacagatacc gtgcggtcgc cgaacgcaac cacgcccaga tccagcgctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for MysmA.01566.b.A1.PS00700
PURIFICATION DATe: 8/2/2010
CONCENTRATION: 45.36mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSIVLTADH GAVRVLTMNR PEARNALGSE LIEALFTALQ DADDDESVRA VVLTGADPAF CAGVDLKQAQ TLGMDYFARF QTHNCIAKTG QMATPIIGAI NGATFTGGLE MALGCDFLIA SERAVFADTH ARVGILPGGG MTARLPQVVG AAMARRLSMT GEVVDASRAE RLGLVTEVVA HEQLLVRALE LAQQIAEVPG PIMSGLKEIY TLGADPVITP ALDAEQQIAG TQQRDFAGLG DRYRAVAERN HAQ
Validated NT Sequence
atctgggcgt ggttgcgttc ggcgaccgca cggtatctgt cgccgagacc ggcgaagtcg cgctgctgcg ttccggcgat ctgctgctcg gcgtccagcg cgggagtgat gaccggatca gcgccaagtg tgtagatctc tttgaggccg gacatgatcg ggcccggtac ctcggcgatc tgctgggcga gttcgagtgc gcggaccagg agctgctcgt gcgccaccac ctcggtgacc aatcccaggc gttcggcgcg tgaggcgtcg accacctcac cggtcatcga caaccggcgc gccattgcgg cgccgaccac ctgcggcagt cgcgccgtca ttccgccgcc gggcagaatc cccacgcgcg catgggtatc ggcgaacacc gcacgttcgg aggcgatcag gaagtcgcag cccagtgcca tctccaggcc gccggtgaac gtcgcgccgt tgatcgcgcc gatgatcggg gtcgccatct gcccggtctt ggcgatgcag ttgtgcgtct ggaaccgcgc gaaatagtcc ataccgagcg tctgcgcctg cttgaggtcg acacccgcgc agaacgccgg gtccgcaccg gtcaacacca cggcccgcac agattcgtcg tcatcggcgt cctgcagcgc ggtgaacagc gcctcgatca gctcgctgcc cagcgcgttg cgggcctcgg gacggttcat ggtgagcacg cggacggcac cgtggtcggc ggtgagcacg atcgacatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca t
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIVLTADH GAVRVLTMNR PEARNALGSE LIEALFTALQ DADDDESVRA VVLTGADPAF CAGVDLKQAQ TLGMDYFARF QTHNCIAKTG QMATPIIGAI NGATFTGGLE MALGCDFLIA SERAVFADTH ARVGILPGGG MTARLPQVVG AAMARRLSMT GEVVDASRAE RLGLVTEVVA HEQLLVRALE LAQQIAEVPG PIMSGLKEIY TLGADPVITP ALDAEQQIAG TQQRDFAGLG DRYRAVAERN HAQIQR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCGATCGTG CTCACCGCCG ACCACGGTGC CGTCCGCGTG CTCACCATGA ACCGTCCCGA GGCCCGCAAC GCGCTGGGCA GCGAGCTGAT CGAGGCGCTG TTCACCGCGC TGCAGGACGC CGATGACGAC GAATCTGTGC GGGCCGTGGT GTTGACCGGT GCGGACCCGG CGTTCTGCGC GGGTGTCGAC CTCAAGCAGG CGCAGACGCT CGGTATGGAC TATTTCGCGC GGTTCCAGAC GCACAACTGC ATCGCCAAGA CCGGGCAGAT GGCGACCCCG ATCATCGGCG CGATCAACGG CGCGACGTTC ACCGGCGGCC TGGAGATGGC ACTGGGCTGC GACTTCCTGA TCGCCTCCGA ACGTGCGGTG TTCGCCGATA CCCATGCGCG CGTGGGGATT CTGCCCGGCG GCGGAATGAC GGCGCGACTG CCGCAGGTGG TCGGCGCCGC AATGGCGCGC CGGTTGTCGA TGACCGGTGA GGTGGTCGAC GCCTCACGCG CCGAACGCCT GGGATTGGTC ACCGAGGTGG TGGCGCACGA GCAGCTCCTG GTCCGCGCAC TCGAACTCGC CCAGCAGATC GCCGAGGTAC CGGGCCCGAT CATGTCCGGC CTCAAAGAGA TCTACACACT TGGCGCTGAT CCGGTCATCA CTCCCGCGCT GGACGCCGAG CAGCAGATCG CCGGAACGCA GCAGCGCGAC TTCGCCGGTC TCGGCGACAG ATACCGTGCG GTCGCCGAAC GCAACCACGC CCAGATCCAG CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA