MysmA.01395.a

3-mercaptopyruvate sulfurtransferase

CENTER ID: MysmA.01395.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01395.a.A1.GE28927 Full length( MysmA.01395.a ) 1 286
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01395.a.A1.PS00840 Full length( MysmA.01395.a ) 1 286
MysmA.01395.a.A1.PS00841 Full length( MysmA.01395.a ) 1 286

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3200
RefSeq: YP_887551.1
UniProt: A0QXB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATTRSDVFI STAELIQLLA AGGPVTLLDV RWTLAEPNGE QAYLDGHLPG AVYVSLDDEL ADHTVRGRGR HPLPSGRHLE AAARRWGVRD GVPTVVYDDW NRAGSARAWW CLTAAGISGV RILDGGLGAW VAGGGGVETG PVTPEPGDVR VVHDDLYRGA LPTLTADDVQ SAAALIDARA PERFRGEVEP VDPVAGHVPG AVNLPSTGLL NPDGTLRDEA QVRALLADRG VDDTGDTAVG AYCGSGVTAA LTVAGLAAAG VDAALFPGSW SEWVCDPGRP VARGEK
NT Sequence
GTGGCGACAA CTCGTTCCGA CGTCTTCATC AGCACCGCGG AGCTCATCCA GCTGCTCGCG GCAGGCGGCC CGGTGACCCT GCTCGACGTG CGCTGGACCC TGGCCGAGCC GAACGGTGAA CAGGCCTATC TCGACGGGCA TCTGCCCGGC GCGGTCTACG TGTCGCTCGA CGACGAGCTC GCCGACCACA CCGTGCGCGG CCGCGGCCGT CACCCACTGC CGTCCGGACG TCACCTCGAG GCCGCCGCGC GCCGCTGGGG CGTGCGCGAC GGTGTCCCGA CCGTGGTGTA CGACGACTGG AACCGCGCGG GTTCGGCGCG TGCCTGGTGG TGTCTGACCG CCGCGGGGAT CAGCGGTGTG CGCATCCTCG ACGGTGGTCT GGGGGCCTGG GTTGCGGGCG GAGGCGGTGT CGAGACCGGT CCGGTGACAC CGGAACCGGG CGACGTCCGC GTCGTCCACG ACGATCTGTA CCGCGGCGCG CTGCCCACGC TGACCGCCGA CGACGTGCAG TCCGCGGCTG CTCTGATCGA CGCCAGGGCG CCCGAGCGGT TCCGCGGTGA GGTCGAGCCG GTCGACCCCG TCGCCGGTCA CGTCCCGGGC GCGGTGAACC TGCCGAGCAC CGGGCTGCTC AATCCCGACG GCACCCTGCG TGACGAGGCA CAGGTGCGTG CGCTTCTCGC CGACCGCGGC GTCGACGACA CCGGGGACAC CGCAGTGGGT GCCTACTGCG GTTCGGGCGT GACGGCCGCG CTCACCGTGG CGGGCCTGGC CGCGGCGGGT GTCGATGCCG CGCTGTTCCC GGGATCCTGG TCGGAGTGGG TCTGCGATCC CGGCCGCCCG GTGGCACGCG GCGAGAAG
Details for MysmA.01395.a.A1.GE28927
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 38
PERCENT COVERAGE: 14
Validated AA Sequence
MRTPLIPAAV RHHQARAEPA RFQSSYTTVG TPSRTPQRRA AASR
Validated NT Sequence
cgccgcgtgc caccgggcgg ccgggatcgc agacccactc cgaccaggat cccgggaaca gcgcggcatc gacacccgcc gcggccaggc ccgccacggt gagcgcggcc gtcacgcccg aaccgcagta ggcacccact gcggtgtccc cggtgtcgtc gacgccgcgg tcggcgagaa gcgcacgcac ctgtgcctcg tcacgcaggg tgccgtcggg attgagcagc ccggtgctcg gcaggttcac cgcgcccggg acgtgaccgg cgacggggtc gaccggctcg acctcaccgc ggaaccgctc gggcgccctg gcgtcgatca gagcagccgc ggactgcacg tcgtcggcgg tcagcgtggg cagcgcgccg cggtacagat cgtcgtggac gacgcggacg tcgcccggtt ccggtgtcac cggaccggtc tcgacaccgc ctccgcccgc aacccaggcc cccagaccac cgtcgaggat gcgcacaccg ctgatccccg cggcggtcag acaccaccag gcacgcgccg aacccgcgcg gttccagtcg tcgtacacca cggtcgggac accgtcgcgc acgccccagc ggcgcgcggc ggcctcgagg tgacgtccgg acggcagtgg gtgacggccg cggccgcgca cggtgtggtc ggcgagctcg tcgtcgagcg acacgtagac cgcgccgggc agatgcccgt cgagataggc ctgttcaccg ttcggctcgg ccagggtcca gcgcacgtcg agcagggtca ccgggccgcc tgccgcgagc agctggatga gctccgcggt gctgatgaag acgtcgga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVATTRSDV FISTAELIQL LAAGGPVTLL DVRWTLAEPN GEQAYLDGHL PGAVYVSLDD ELADHTVRGR GRHPLPSGRH LEAAARRWGV RDGVPTVVYD DWNRAGSARA WWCLTAAGIS GVRILDGGLG AWVAGGGGVE TGPVTPEPGD VRVVHDDLYR GALPTLTADD VQSAAALIDA RAPERFRGEV EPVDPVAGHV PGAVNLPSTG LLNPDGTLRD EAQVRALLAD RGVDDTGDTA VGAYCGSGVT AALTVAGLAA AGVDAALFPG SWSEWVCDPG RPVARGEK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgaca actcgttccg acgtcttcat cagcaccgcg gagctcatcc agctgctcgc ggcaggcggc ccggtgaccc tgctcgacgt gcgctggacc ctggccgagc cgaacggtga acaggcctat ctcgacgggc atctgcccgg cgcggtctac gtgtcgctcg acgacgagct cgccgaccac accgtgcgcg gccgcggccg tcacccactg ccgtccggac gtcacctcga ggccgccgcg cgccgctggg gcgtgcgcga cggtgtcccg accgtggtgt acgacgactg gaaccgcgcg ggttcggcgc gtgcctggtg gtgtctgacc gccgcgggga tcagcggtgt gcgcatcctc gacggtggtc tgggggcctg ggttgcgggc ggaggcggtg tcgagaccgg tccggtgaca ccggaaccgg gcgacgtccg cgtcgtccac gacgatctgt accgcggcgc gctgcccacg ctgaccgccg acgacgtgca gtccgcggct gctctgatcg acgccagggc gcccgagcgg ttccgcggtg aggtcgagcc ggtcgacccc gtcgccggtc acgtcccggg cgcggtgaac ctgccgagca ccgggctgct caatcccgac ggcaccctgc gtgacgaggc acaggtgcgt gcgcttctcg ccgaccgcgg cgtcgacgac accggggaca ccgcagtggg tgcctactgc ggttcgggcg tgacggccgc gctcaccgtg gcgggcctgg ccgcggcggg tgtcgatgcc gcgctgttcc cgggatcctg gtcggagtgg gtctgcgatc ccggccgccc ggtggcacgc ggcgagaaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.01395.a.A1.PS00840
PURIFICATION DATe: 10/26/2010
CONCENTRATION: 34.85mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 38
PERCENT COVERAGE: 14
Protocol Notes
notes unavailable
Validated AA Sequence
MRTPLIPAAV RHHQARAEPA RFQSSYTTVG TPSRTPQRRA AASR
Validated NT Sequence
cgccgcgtgc caccgggcgg ccgggatcgc agacccactc cgaccaggat cccgggaaca gcgcggcatc gacacccgcc gcggccaggc ccgccacggt gagcgcggcc gtcacgcccg aaccgcagta ggcacccact gcggtgtccc cggtgtcgtc gacgccgcgg tcggcgagaa gcgcacgcac ctgtgcctcg tcacgcaggg tgccgtcggg attgagcagc ccggtgctcg gcaggttcac cgcgcccggg acgtgaccgg cgacggggtc gaccggctcg acctcaccgc ggaaccgctc gggcgccctg gcgtcgatca gagcagccgc ggactgcacg tcgtcggcgg tcagcgtggg cagcgcgccg cggtacagat cgtcgtggac gacgcggacg tcgcccggtt ccggtgtcac cggaccggtc tcgacaccgc ctccgcccgc aacccaggcc cccagaccac cgtcgaggat gcgcacaccg ctgatccccg cggcggtcag acaccaccag gcacgcgccg aacccgcgcg gttccagtcg tcgtacacca cggtcgggac accgtcgcgc acgccccagc ggcgcgcggc ggcctcgagg tgacgtccgg acggcagtgg gtgacggccg cggccgcgca cggtgtggtc ggcgagctcg tcgtcgagcg acacgtagac cgcgccgggc agatgcccgt cgagataggc ctgttcaccg ttcggctcgg ccagggtcca gcgcacgtcg agcagggtca ccgggccgcc tgccgcgagc agctggatga gctccgcggt gctgatgaag acgtcgga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVATTRSDV FISTAELIQL LAAGGPVTLL DVRWTLAEPN GEQAYLDGHL PGAVYVSLDD ELADHTVRGR GRHPLPSGRH LEAAARRWGV RDGVPTVVYD DWNRAGSARA WWCLTAAGIS GVRILDGGLG AWVAGGGGVE TGPVTPEPGD VRVVHDDLYR GALPTLTADD VQSAAALIDA RAPERFRGEV EPVDPVAGHV PGAVNLPSTG LLNPDGTLRD EAQVRALLAD RGVDDTGDTA VGAYCGSGVT AALTVAGLAA AGVDAALFPG SWSEWVCDPG RPVARGEK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCGACA ACTCGTTCCG ACGTCTTCAT CAGCACCGCG GAGCTCATCC AGCTGCTCGC GGCAGGCGGC CCGGTGACCC TGCTCGACGT GCGCTGGACC CTGGCCGAGC CGAACGGTGA ACAGGCCTAT CTCGACGGGC ATCTGCCCGG CGCGGTCTAC GTGTCGCTCG ACGACGAGCT CGCCGACCAC ACCGTGCGCG GCCGCGGCCG TCACCCACTG CCGTCCGGAC GTCACCTCGA GGCCGCCGCG CGCCGCTGGG GCGTGCGCGA CGGTGTCCCG ACCGTGGTGT ACGACGACTG GAACCGCGCG GGTTCGGCGC GTGCCTGGTG GTGTCTGACC GCCGCGGGGA TCAGCGGTGT GCGCATCCTC GACGGTGGTC TGGGGGCCTG GGTTGCGGGC GGAGGCGGTG TCGAGACCGG TCCGGTGACA CCGGAACCGG GCGACGTCCG CGTCGTCCAC GACGATCTGT ACCGCGGCGC GCTGCCCACG CTGACCGCCG ACGACGTGCA GTCCGCGGCT GCTCTGATCG ACGCCAGGGC GCCCGAGCGG TTCCGCGGTG AGGTCGAGCC GGTCGACCCC GTCGCCGGTC ACGTCCCGGG CGCGGTGAAC CTGCCGAGCA CCGGGCTGCT CAATCCCGAC GGCACCCTGC GTGACGAGGC ACAGGTGCGT GCGCTTCTCG CCGACCGCGG CGTCGACGAC ACCGGGGACA CCGCAGTGGG TGCCTACTGC GGTTCGGGCG TGACGGCCGC GCTCACCGTG GCGGGCCTGG CCGCGGCGGG TGTCGATGCC GCGCTGTTCC CGGGATCCTG GTCGGAGTGG GTCTGCGATC CCGGCCGCCC GGTGGCACGC GGCGAGAAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A
Details for MysmA.01395.a.A1.PS00841
PURIFICATION DATe: 10/26/2010
CONCENTRATION: 44.15mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: 38
PERCENT COVERAGE: 14
Protocol Notes
notes unavailable
Validated AA Sequence
MRTPLIPAAV RHHQARAEPA RFQSSYTTVG TPSRTPQRRA AASR
Validated NT Sequence
cgccgcgtgc caccgggcgg ccgggatcgc agacccactc cgaccaggat cccgggaaca gcgcggcatc gacacccgcc gcggccaggc ccgccacggt gagcgcggcc gtcacgcccg aaccgcagta ggcacccact gcggtgtccc cggtgtcgtc gacgccgcgg tcggcgagaa gcgcacgcac ctgtgcctcg tcacgcaggg tgccgtcggg attgagcagc ccggtgctcg gcaggttcac cgcgcccggg acgtgaccgg cgacggggtc gaccggctcg acctcaccgc ggaaccgctc gggcgccctg gcgtcgatca gagcagccgc ggactgcacg tcgtcggcgg tcagcgtggg cagcgcgccg cggtacagat cgtcgtggac gacgcggacg tcgcccggtt ccggtgtcac cggaccggtc tcgacaccgc ctccgcccgc aacccaggcc cccagaccac cgtcgaggat gcgcacaccg ctgatccccg cggcggtcag acaccaccag gcacgcgccg aacccgcgcg gttccagtcg tcgtacacca cggtcgggac accgtcgcgc acgccccagc ggcgcgcggc ggcctcgagg tgacgtccgg acggcagtgg gtgacggccg cggccgcgca cggtgtggtc ggcgagctcg tcgtcgagcg acacgtagac cgcgccgggc agatgcccgt cgagataggc ctgttcaccg ttcggctcgg ccagggtcca gcgcacgtcg agcagggtca ccgggccgcc tgccgcgagc agctggatga gctccgcggt gctgatgaag acgtcgga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVATTRSDV FISTAELIQL LAAGGPVTLL DVRWTLAEPN GEQAYLDGHL PGAVYVSLDD ELADHTVRGR GRHPLPSGRH LEAAARRWGV RDGVPTVVYD DWNRAGSARA WWCLTAAGIS GVRILDGGLG AWVAGGGGVE TGPVTPEPGD VRVVHDDLYR GALPTLTADD VQSAAALIDA RAPERFRGEV EPVDPVAGHV PGAVNLPSTG LLNPDGTLRD EAQVRALLAD RGVDDTGDTA VGAYCGSGVT AALTVAGLAA AGVDAALFPG SWSEWVCDPG RPVARGEK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCGACA ACTCGTTCCG ACGTCTTCAT CAGCACCGCG GAGCTCATCC AGCTGCTCGC GGCAGGCGGC CCGGTGACCC TGCTCGACGT GCGCTGGACC CTGGCCGAGC CGAACGGTGA ACAGGCCTAT CTCGACGGGC ATCTGCCCGG CGCGGTCTAC GTGTCGCTCG ACGACGAGCT CGCCGACCAC ACCGTGCGCG GCCGCGGCCG TCACCCACTG CCGTCCGGAC GTCACCTCGA GGCCGCCGCG CGCCGCTGGG GCGTGCGCGA CGGTGTCCCG ACCGTGGTGT ACGACGACTG GAACCGCGCG GGTTCGGCGC GTGCCTGGTG GTGTCTGACC GCCGCGGGGA TCAGCGGTGT GCGCATCCTC GACGGTGGTC TGGGGGCCTG GGTTGCGGGC GGAGGCGGTG TCGAGACCGG TCCGGTGACA CCGGAACCGG GCGACGTCCG CGTCGTCCAC GACGATCTGT ACCGCGGCGC GCTGCCCACG CTGACCGCCG ACGACGTGCA GTCCGCGGCT GCTCTGATCG ACGCCAGGGC GCCCGAGCGG TTCCGCGGTG AGGTCGAGCC GGTCGACCCC GTCGCCGGTC ACGTCCCGGG CGCGGTGAAC CTGCCGAGCA CCGGGCTGCT CAATCCCGAC GGCACCCTGC GTGACGAGGC ACAGGTGCGT GCGCTTCTCG CCGACCGCGG CGTCGACGAC ACCGGGGACA CCGCAGTGGG TGCCTACTGC GGTTCGGGCG TGACGGCCGC GCTCACCGTG GCGGGCCTGG CCGCGGCGGG TGTCGATGCC GCGCTGTTCC CGGGATCCTG GTCGGAGTGG GTCTGCGATC CCGGCCGCCC GGTGGCACGC GGCGAGAAGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A