SSGCID: Target Page

MysmA.01370.a

Phosphomannomutase/phosphoglucomutase

CENTER ID:	MysmA.01370.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01370.a.A1.GE28923	Full length( MysmA.01370.a )		1	472

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01370.a.A1.PW28828	Full length( MysmA.01370.a )		1	472

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.1816
RefSeq:	YP_886204.1
UniProt:	A0QTG6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSRPAAAVHG VIKAYDVRGL VGSELDESFV ADVGGAFARL VRGEGARQVV IGHDMRSSSP ALAAAFADGV TTQGLDVVRI GLASTDQLYF ASGLLECPGA MFTASHNPAA YNGIKLCRAG AKPVGKDTGL AAISEEVIGG VPAHDGPRGG ITDRDVLADY GEFLRSLVDL SGSRPLRIAV DAGNGMAGHT TPAVLGPIPG ATILPLYFEL DGTFPNHEAN PLDPANLVDL QRFVVESGAD IGLAFDGDAD RCFVVDELGR PVSPSAVTAL VAGRELTREI GATIIHNLIT SRAVPELVTE RGGTPVRARV GHSYIKALMA ETSAIFGGEH SAHYYFRDFW GADSGMLAAL HVLAALGEQD RPLSEIMADY QRYEASGEIN FTVSDASACV DAVLTSYGTA IHSIDHLDGV TVDLGDDCWF NLRSSNTEPL LRLNVEARTT EEVDAVVDRI ADVIRATNEP GSVNGAVNEA AT

NT Sequence

ATGTCTCGGC CAGCAGCGGC TGTTCACGGT GTCATCAAGG CCTATGACGT CCGCGGTCTG GTCGGCTCCG AGTTGGACGA GTCGTTCGTC GCCGACGTGG GCGGCGCGTT TGCCCGGTTG GTCCGCGGCG AAGGCGCCCG GCAGGTCGTC ATCGGCCACG ACATGCGATC CAGCTCGCCG GCGCTGGCGG CGGCTTTCGC CGACGGCGTC ACCACGCAGG GACTCGACGT CGTGCGGATC GGTCTCGCCT CGACCGATCA GCTCTACTTC GCGTCCGGGT TGTTGGAGTG CCCGGGCGCG ATGTTCACCG CGAGCCACAA CCCCGCGGCC TACAACGGCA TCAAGCTGTG CCGCGCGGGC GCCAAACCGG TGGGCAAGGA CACCGGGCTG GCCGCCATCT CCGAGGAGGT TATCGGCGGC GTACCGGCCC ATGACGGCCC GCGGGGCGGC ATCACCGACC GCGACGTACT GGCCGACTAC GGCGAGTTCC TGCGTTCCCT GGTCGATCTC AGCGGCTCAC GGCCCCTGCG CATCGCCGTC GACGCAGGCA ACGGCATGGC CGGGCACACC ACGCCCGCTG TGCTCGGGCC GATCCCCGGG GCCACGATCC TGCCGCTGTA CTTCGAACTC GACGGCACGT TCCCCAACCA CGAGGCCAAC CCGCTCGACC CGGCCAACCT GGTGGACCTG CAGCGCTTCG TTGTGGAGTC CGGCGCCGAC ATCGGGCTCG CGTTCGACGG CGACGCCGAC CGCTGTTTCG TCGTCGACGA ACTCGGTCGC CCCGTCTCGC CGTCGGCCGT GACGGCTCTG GTCGCCGGTC GCGAACTCAC CCGTGAGATC GGCGCGACGA TCATCCACAA CCTCATCACC TCGCGCGCGG TGCCCGAGCT CGTGACCGAG CGGGGCGGCA CCCCGGTGCG CGCGCGGGTC GGGCATTCCT ACATCAAGGC GCTGATGGCC GAGACCAGCG CGATATTCGG CGGTGAGCAC TCGGCCCACT ACTACTTCCG CGACTTCTGG GGCGCCGACT CGGGAATGCT CGCGGCCCTG CACGTGCTCG CCGCGCTCGG CGAACAGGAC CGCCCGCTTT CGGAGATCAT GGCCGACTAT CAGCGCTACG AGGCCTCGGG CGAGATCAAC TTCACCGTCA GCGACGCATC GGCTTGCGTG GACGCGGTGC TCACGTCGTA CGGCACGGCC ATCCACTCCA TCGACCACCT CGACGGCGTC ACGGTCGACC TCGGCGACGA CTGCTGGTTC AACCTGCGCA GCTCGAACAC CGAACCGCTG CTGCGCCTCA ACGTCGAGGC GCGAACCACC GAGGAGGTCG ACGCAGTGGT CGACAGAATC GCCGACGTCA TCCGCGCCAC CAACGAACCC GGCAGCGTGA ACGGGGCGGT GAACGAGGCG GCGACG

Details for MysmA.01370.a.A1.GE28923

HARVESTED ON:	3/30/2010
SEQUENCED ON:	4/20/2010
EXPECTED MW:	51kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	19
PERCENT COVERAGE:	36

Validated AA Sequence

MSXXXXXVHG XSRPMTSAVW SAPSWTSRSS PTWAXRLPGW SAAKAPGRSS SATTCDPARR RWRRLSPTXS PRRDSTSXGS XSPXPISSTS RPGCWSARXR CSPRATTPXP TTXSSCAARX PNRXXRTPGW PPSXRRLSAA XRXMXXRXAX XXXXXXXXXX XSSXXXWSIX XXHXPCAXXX

Validated NT Sequence

attttgtnnn ntttaagaag gagatatncc ntggatcatc accatcacca tnnnnnnnnn nnnnnnngct cagacccngg gtcctggttc gatgtctnnn nnnnnnncgg ntgttcacgg ngnntcaagg cctatgacgt ccgcggtctg gtcggctccg agttggacga gtcgttcgtc gccnacgtgg gcggngcgtt tgcccggttg gtccgcggcg aaggcgcccg gcaggtcgtc atcggccacg acatgcgatc cagctcgccg gcgctggcgg cggctttcgc cgacggngtc accacgcagg gactcgacgt cgngcggatc ggnctcnccn cnaccgatca gctctacttc gcgtccgggt tgttggagtg cccgggngcg atgttcaccg cgagccacaa ccccgnggcc tacaacggnn tcaagctgtg ccgcgcgggn nccnaaccgg nggncnagga caccgggntg gccgccntcn nnnaggaggt tatcggcggc gtnncgnncn atgnnnncnc gngnngcgnc ntnncnnnnt nnnanntant gncngantnc ngnnagttct tgnnntcnct ggtcnatann ancggntcac nncccctgcg ccnnngncaa ntca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSRPAAAVH GVIKAYDVRG LVGSELDESF VADVGGAFAR LVRGEGARQV VIGHDMRSSS PALAAAFADG VTTQGLDVVR IGLASTDQLY FASGLLECPG AMFTASHNPA AYNGIKLCRA GAKPVGKDTG LAAISEEVIG GVPAHDGPRG GITDRDVLAD YGEFLRSLVD LSGSRPLRIA VDAGNGMAGH TTPAVLGPIP GATILPLYFE LDGTFPNHEA NPLDPANLVD LQRFVVESGA DIGLAFDGDA DRCFVVDELG RPVSPSAVTA LVAGRELTRE IGATIIHNLI TSRAVPELVT ERGGTPVRAR VGHSYIKALM AETSAIFGGE HSAHYYFRDF WGADSGMLAA LHVLAALGEQ DRPLSEIMAD YQRYEASGEI NFTVSDASAC VDAVLTSYGT AIHSIDHLDG VTVDLGDDCW FNLRSSNTEP LLRLNVEART TEEVDAVVDR IADVIRATNE PGSVNGAVNE AAT

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctcggcca gcagcggctg ttcacggtgt catcaaggcc tatgacgtcc gcggtctggt cggctccgag ttggacgagt cgttcgtcgc cgacgtgggc ggcgcgtttg cccggttggt ccgcggcgaa ggcgcccggc aggtcgtcat cggccacgac atgcgatcca gctcgccggc gctggcggcg gctttcgccg acggcgtcac cacgcaggga ctcgacgtcg tgcggatcgg tctcgcctcg accgatcagc tctacttcgc gtccgggttg ttggagtgcc cgggcgcgat gttcaccgcg agccacaacc ccgcggccta caacggcatc aagctgtgcc gcgcgggcgc caaaccggtg ggcaaggaca ccgggctggc cgccatctcc gaggaggtta tcggcggcgt accggcccat gacggcccgc ggggcggcat caccgaccgc gacgtactgg ccgactacgg cgagttcctg cgttccctgg tcgatctcag cggctcacgg cccctgcgca tcgccgtcga cgcaggcaac ggcatggccg ggcacaccac gcccgctgtg ctcgggccga tccccggggc cacgatcctg ccgctgtact tcgaactcga cggcacgttc cccaaccacg aggccaaccc gctcgacccg gccaacctgg tggacctgca gcgcttcgtt gtggagtccg gcgccgacat cgggctcgcg ttcgacggcg acgccgaccg ctgtttcgtc gtcgacgaac tcggtcgccc cgtctcgccg tcggccgtga cggctctggt cgccggtcgc gaactcaccc gtgagatcgg cgcgacgatc atccacaacc tcatcacctc gcgcgcggtg cccgagctcg tgaccgagcg gggcggcacc ccggtgcgcg cgcgggtcgg gcattcctac atcaaggcgc tgatggccga gaccagcgcg atattcggcg gtgagcactc ggcccactac tacttccgcg acttctgggg cgccgactcg ggaatgctcg cggccctgca cgtgctcgcc gcgctcggcg aacaggaccg cccgctttcg gagatcatgg ccgactatca gcgctacgag gcctcgggcg agatcaactt caccgtcagc gacgcatcgg cttgcgtgga cgcggtgctc acgtcgtacg gcacggccat ccactccatc gaccacctcg acggcgtcac ggtcgacctc ggcgacgact gctggttcaa cctgcgcagc tcgaacaccg aaccgctgct gcgcctcaac gtcgaggcgc gaaccaccga ggaggtcgac gcagtggtcg acagaatcgc cgacgtcatc cgcgccacca acgaacccgg cagcgtgaac ggggcggtga acgaggcggc gacgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa

Details for MysmA.01370.a.A1.PW28828

PURIFICATION DATe:	11/1/2010
CONCENTRATION:	25.77mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	100µl
PERCENT IDENTITY:	19
PERCENT COVERAGE:	36

Protocol Notes

notes unavailable

Validated AA Sequence

MSXXXXXVHG XSRPMTSAVW SAPSWTSRSS PTWAXRLPGW SAAKAPGRSS SATTCDPARR RWRRLSPTXS PRRDSTSXGS XSPXPISSTS RPGCWSARXR CSPRATTPXP TTXSSCAARX PNRXXRTPGW PPSXRRLSAA XRXMXXRXAX XXXXXXXXXX XSSXXXWSIX XXHXPCAXXX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTCGGCCA GCAGCGGCTG TTCACGGTGT CATCAAGGCC TATGACGTCC GCGGTCTGGT CGGCTCCGAG TTGGACGAGT CGTTCGTCGC CGACGTGGGC GGCGCGTTTG CCCGGTTGGT CCGCGGCGAA GGCGCCCGGC AGGTCGTCAT CGGCCACGAC ATGCGATCCA GCTCGCCGGC GCTGGCGGCG GCTTTCGCCG ACGGCGTCAC CACGCAGGGA CTCGACGTCG TGCGGATCGG TCTCGCCTCG ACCGATCAGC TCTACTTCGC GTCCGGGTTG TTGGAGTGCC CGGGCGCGAT GTTCACCGCG AGCCACAACC CCGCGGCCTA CAACGGCATC AAGCTGTGCC GCGCGGGCGC CAAACCGGTG GGCAAGGACA CCGGGCTGGC CGCCATCTCC GAGGAGGTTA TCGGCGGCGT ACCGGCCCAT GACGGCCCGC GGGGCGGCAT CACCGACCGC GACGTACTGG CCGACTACGG CGAGTTCCTG CGTTCCCTGG TCGATCTCAG CGGCTCACGG CCCCTGCGCA TCGCCGTCGA CGCAGGCAAC GGCATGGCCG GGCACACCAC GCCCGCTGTG CTCGGGCCGA TCCCCGGGGC CACGATCCTG CCGCTGTACT TCGAACTCGA CGGCACGTTC CCCAACCACG AGGCCAACCC GCTCGACCCG GCCAACCTGG TGGACCTGCA GCGCTTCGTT GTGGAGTCCG GCGCCGACAT CGGGCTCGCG TTCGACGGCG ACGCCGACCG CTGTTTCGTC GTCGACGAAC TCGGTCGCCC CGTCTCGCCG TCGGCCGTGA CGGCTCTGGT CGCCGGTCGC GAACTCACCC GTGAGATCGG CGCGACGATC ATCCACAACC TCATCACCTC GCGCGCGGTG CCCGAGCTCG TGACCGAGCG GGGCGGCACC CCGGTGCGCG CGCGGGTCGG GCATTCCTAC ATCAAGGCGC TGATGGCCGA GACCAGCGCG ATATTCGGCG GTGAGCACTC GGCCCACTAC TACTTCCGCG ACTTCTGGGG CGCCGACTCG GGAATGCTCG CGGCCCTGCA CGTGCTCGCC GCGCTCGGCG AACAGGACCG CCCGCTTTCG GAGATCATGG CCGACTATCA GCGCTACGAG GCCTCGGGCG AGATCAACTT CACCGTCAGC GACGCATCGG CTTGCGTGGA CGCGGTGCTC ACGTCGTACG GCACGGCCAT CCACTCCATC GACCACCTCG ACGGCGTCAC GGTCGACCTC GGCGACGACT GCTGGTTCAA CCTGCGCAGC TCGAACACCG AACCGCTGCT GCGCCTCAAC GTCGAGGCGC GAACCACCGA GGAGGTCGAC GCAGTGGTCG ACAGAATCGC CGACGTCATC CGCGCCACCA ACGAACCCGG CAGCGTGAAC GGGGCGGTGA ACGAGGCGGC GACGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA