MysmA.01365.g
L-xylulose reductase
| CENTER ID: | MysmA.01365.g |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01365.g.A1.GE28921 | Full length( MysmA.01365.g ) | 1 | 262 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01365.g.A1.PW28826 | Full length( MysmA.01365.g ) | 1 | 262 | unavailable |
Structures
4EGF
DEPOSITED: 3/30/2012
DETERMINATION: XRay
CLONE: MysmA.01365.g.A1.GE28921
PROTEIN: MysmA.01365.g.A1.PW28826
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.3224 |
| RefSeq: | YP_887574.1 |
| UniProt: | A0QXD6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTTDDRYAG VLRLDGKRAL ITGATKGIGA DIARAFAAAG ARLVLSGRDV SELDAARRAL GEQFGTDVHT VAIDLAEPDA PAELARRAAE AFGGLDVLVN NAGISHPQPV VDTDPQLFDA TIAVNLRAPA LLASAVGKAM VAAGEGGAII TVASAAALAP LPDHYAYCTS KAGLVMATKV LARELGPHGI RANSVCPTVV LTEMGQRVWG DEAKSAPMIA RIPLGRFAVP HEVSDAVVWL ASDAASMING VDIPVDGGYT MG
NT Sequence
GTGACCACCA CCGACGACCG CTACGCCGGC GTTCTGCGCC TCGACGGCAA ACGCGCCCTG ATCACCGGTG CCACCAAGGG CATCGGCGCC GACATCGCCC GCGCGTTCGC CGCTGCGGGC GCACGGCTTG TGCTCAGCGG CCGCGACGTG AGCGAACTCG ACGCCGCGCG AAGGGCTCTC GGCGAACAGT TCGGCACCGA CGTGCACACC GTCGCGATCG ATCTCGCCGA ACCCGACGCA CCCGCCGAGT TGGCCCGGCG CGCCGCCGAG GCGTTCGGTG GTCTGGACGT GCTGGTGAAC AACGCCGGGA TCTCGCATCC ACAGCCCGTC GTCGACACCG ATCCCCAGCT GTTCGACGCC ACCATCGCGG TCAACCTGCG CGCCCCGGCA CTGCTGGCCT CGGCGGTGGG CAAAGCCATG GTGGCGGCAG GCGAGGGCGG GGCGATCATC ACGGTGGCCT CGGCCGCCGC GCTGGCACCT CTGCCCGACC ACTACGCGTA CTGCACGTCC AAGGCCGGCC TGGTGATGGC GACCAAGGTG CTTGCCCGCG AGCTCGGTCC CCACGGCATC CGCGCCAACT CGGTGTGCCC GACGGTGGTG CTGACCGAGA TGGGTCAGCG GGTGTGGGGC GACGAGGCCA AGTCGGCGCC GATGATCGCA CGGATCCCGT TGGGCCGCTT CGCGGTTCCT CATGAGGTTT CCGACGCGGT GGTGTGGCTC GCCTCCGACG CGGCCAGCAT GATCAACGGC GTCGACATCC CCGTCGACGG CGGCTACACG ATGGGC
Details for MysmA.01365.g.A1.GE28921
| HARVESTED ON: | 3/30/2010 |
| SEQUENCED ON: | 4/20/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLXAQTQGPG SMVTTTDDRY AGVLRLDGKR ALITGATKGI GADIARAFAA AGARLVLSGR DVSELDAARR ALGEQFGTDV HTVAIDLAEP DAPAELARRA AEAFGGLDVL VNNAGISHPQ PVVDTDPQLF DATIAVNLRA PALLASAVGK AMVAAGEGGA IITVASAAAL APLPDHYAYC TSKAGLVMAT KVLARELGPH GIRANSVCPT VVLTEMGQRV WGDEAKSAPM IARIPLGRFA VPHEVSDAVV WLASDAASMI NGVDIPVDGG YTMG
Validated NT Sequence
ttattagccc atcgtgtagc cgccgtcgac ggggatgtcg acgccgttga tcatgctggc cgcgtcggag gcgagccaca ccaccgcgtc ggaaacctca tgaggaaccg cgaagcggcc caacgggatc cgtgcgatca tcggcgccga cttggcctcg tcgccccaca cccgctgacc catctcggtc agcaccaccg tcgggcacac cgagttggcg cggatgccgt ggggaccgag ctcgcgggca agcaccttgg tcgccatcac caggccggcc ttggacgtgc agtacgcgta gtggtcgggc agaggtgcca gcgcggcggc cgaggccacc gtgatgatcg ccccgccctc gcctgccgcc accatggctt tgcccaccgc cgaggccagc agtgccgggg cgcgcaggtt gaccgcgatg gtggcgtcga acagctgggg atcggtgtcg acgacgggct gtggatgcga gatcccggcg ttgttcacca gcacgtccag accaccgaac gcctcggcgg cgcgccgggc caactcggcg ggtgcgtcgg gttcggcgag atcgatcgcg acggtgtgca cgtcggtgcc gaactgttcg ccgagagccc ttcgcgcggc gtcgagttcg ctcacgtcgc ggccgctgag cacaagccgt gcgcccgcag cggcgaacgc gcgggcgatg tcggcgccga tgcccttggt ggcaccggtg atcagggcgc gtttgccgtc gaggcgcaga acgccggcgt agcggtcgtc ggtggtggtc accatcgaac caggaccctg ggtctgagct nccagggtac ccatatgatg gtgatggtga tgagccatgg tat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTTDDRY AGVLRLDGKR ALITGATKGI GADIARAFAA AGARLVLSGR DVSELDAARR ALGEQFGTDV HTVAIDLAEP DAPAELARRA AEAFGGLDVL VNNAGISHPQ PVVDTDPQLF DATIAVNLRA PALLASAVGK AMVAAGEGGA IITVASAAAL APLPDHYAYC TSKAGLVMAT KVLARELGPH GIRANSVCPT VVLTEMGQRV WGDEAKSAPM IARIPLGRFA VPHEVSDAVV WLASDAASMI NGVDIPVDGG YTMG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccacc accgacgacc gctacgccgg cgttctgcgc ctcgacggca aacgcgccct gatcaccggt gccaccaagg gcatcggcgc cgacatcgcc cgcgcgttcg ccgctgcggg cgcacggctt gtgctcagcg gccgcgacgt gagcgaactc gacgccgcgc gaagggctct cggcgaacag ttcggcaccg acgtgcacac cgtcgcgatc gatctcgccg aacccgacgc acccgccgag ttggcccggc gcgccgccga ggcgttcggt ggtctggacg tgctggtgaa caacgccggg atctcgcatc cacagcccgt cgtcgacacc gatccccagc tgttcgacgc caccatcgcg gtcaacctgc gcgccccggc actgctggcc tcggcggtgg gcaaagccat ggtggcggca ggcgagggcg gggcgatcat cacggtggcc tcggccgccg cgctggcacc tctgcccgac cactacgcgt actgcacgtc caaggccggc ctggtgatgg cgaccaaggt gcttgcccgc gagctcggtc cccacggcat ccgcgccaac tcggtgtgcc cgacggtggt gctgaccgag atgggtcagc gggtgtgggg cgacgaggcc aagtcggcgc cgatgatcgc acggatcccg ttgggccgct tcgcggttcc tcatgaggtt tccgacgcgg tggtgtggct cgcctccgac gcggccagca tgatcaacgg cgtcgacatc cccgtcgacg gcggctacac gatgggcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MysmA.01365.g.A1.PW28826
| PURIFICATION DATe: | 7/5/2010 |
| CONCENTRATION: | 23.16mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | data unavailable |
| VIAL VOLUME: | data unavailable |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLXAQTQGPG SMVTTTDDRY AGVLRLDGKR ALITGATKGI GADIARAFAA AGARLVLSGR DVSELDAARR ALGEQFGTDV HTVAIDLAEP DAPAELARRA AEAFGGLDVL VNNAGISHPQ PVVDTDPQLF DATIAVNLRA PALLASAVGK AMVAAGEGGA IITVASAAAL APLPDHYAYC TSKAGLVMAT KVLARELGPH GIRANSVCPT VVLTEMGQRV WGDEAKSAPM IARIPLGRFA VPHEVSDAVV WLASDAASMI NGVDIPVDGG YTMG
Validated NT Sequence
ttattagccc atcgtgtagc cgccgtcgac ggggatgtcg acgccgttga tcatgctggc cgcgtcggag gcgagccaca ccaccgcgtc ggaaacctca tgaggaaccg cgaagcggcc caacgggatc cgtgcgatca tcggcgccga cttggcctcg tcgccccaca cccgctgacc catctcggtc agcaccaccg tcgggcacac cgagttggcg cggatgccgt ggggaccgag ctcgcgggca agcaccttgg tcgccatcac caggccggcc ttggacgtgc agtacgcgta gtggtcgggc agaggtgcca gcgcggcggc cgaggccacc gtgatgatcg ccccgccctc gcctgccgcc accatggctt tgcccaccgc cgaggccagc agtgccgggg cgcgcaggtt gaccgcgatg gtggcgtcga acagctgggg atcggtgtcg acgacgggct gtggatgcga gatcccggcg ttgttcacca gcacgtccag accaccgaac gcctcggcgg cgcgccgggc caactcggcg ggtgcgtcgg gttcggcgag atcgatcgcg acggtgtgca cgtcggtgcc gaactgttcg ccgagagccc ttcgcgcggc gtcgagttcg ctcacgtcgc ggccgctgag cacaagccgt gcgcccgcag cggcgaacgc gcgggcgatg tcggcgccga tgcccttggt ggcaccggtg atcagggcgc gtttgccgtc gaggcgcaga acgccggcgt agcggtcgtc ggtggtggtc accatcgaac caggaccctg ggtctgagct nccagggtac ccatatgatg gtgatggtga tgagccatgg tat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTTDDRY AGVLRLDGKR ALITGATKGI GADIARAFAA AGARLVLSGR DVSELDAARR ALGEQFGTDV HTVAIDLAEP DAPAELARRA AEAFGGLDVL VNNAGISHPQ PVVDTDPQLF DATIAVNLRA PALLASAVGK AMVAAGEGGA IITVASAAAL APLPDHYAYC TSKAGLVMAT KVLARELGPH GIRANSVCPT VVLTEMGQRV WGDEAKSAPM IARIPLGRFA VPHEVSDAVV WLASDAASMI NGVDIPVDGG YTMG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACCACC ACCGACGACC GCTACGCCGG CGTTCTGCGC CTCGACGGCA AACGCGCCCT GATCACCGGT GCCACCAAGG GCATCGGCGC CGACATCGCC CGCGCGTTCG CCGCTGCGGG CGCACGGCTT GTGCTCAGCG GCCGCGACGT GAGCGAACTC GACGCCGCGC GAAGGGCTCT CGGCGAACAG TTCGGCACCG ACGTGCACAC CGTCGCGATC GATCTCGCCG AACCCGACGC ACCCGCCGAG TTGGCCCGGC GCGCCGCCGA GGCGTTCGGT GGTCTGGACG TGCTGGTGAA CAACGCCGGG ATCTCGCATC CACAGCCCGT CGTCGACACC GATCCCCAGC TGTTCGACGC CACCATCGCG GTCAACCTGC GCGCCCCGGC ACTGCTGGCC TCGGCGGTGG GCAAAGCCAT GGTGGCGGCA GGCGAGGGCG GGGCGATCAT CACGGTGGCC TCGGCCGCCG CGCTGGCACC TCTGCCCGAC CACTACGCGT ACTGCACGTC CAAGGCCGGC CTGGTGATGG CGACCAAGGT GCTTGCCCGC GAGCTCGGTC CCCACGGCAT CCGCGCCAAC TCGGTGTGCC CGACGGTGGT GCTGACCGAG ATGGGTCAGC GGGTGTGGGG CGACGAGGCC AAGTCGGCGC CGATGATCGC ACGGATCCCG TTGGGCCGCT TCGCGGTTCC TCATGAGGTT TCCGACGCGG TGGTGTGGCT CGCCTCCGAC GCGGCCAGCA TGATCAACGG CGTCGACATC CCCGTCGACG GCGGCTACAC GATGGGCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA