MysmA.01365.c

Dehydrogenase/reductase SDR family protein member 10

CENTER ID: MysmA.01365.c
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01365.c.A1.GE28917 Full length( MysmA.01365.c ) 1 253
MysmA.01365.c.B1.GE43087 Full length( MysmA.01365.c ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MysmA.01365.c.A1.PW28822 Full length( MysmA.01365.c ) 1 253

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4298
RefSeq: YP_888657.1
UniProt: A0R0G9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLGLTGKRF VVTGGTRGIG RAVVEGLLAE GAAVAFCART PEAVTATQQE LAADGVTVVG TPVDVGDGAA VADWVAQSAE TLGGLDGVVA NVSALAIPES PENWRTSFEV DMMGTVGLVD AALPHLLKSD AGSIVTISSV SGREIDFAAG PYGTFKAAII HYTQGLAYQL AAKGVRANSV SPGNTYFEGG VWPTIEKDDP ELFASALALN PTGRMGTPRE VANAVVFLSS PVASFITGTN LVVDGALTRG VQF
NT Sequence
ATGGATCTTG GACTGACCGG TAAACGCTTC GTGGTCACGG GCGGCACGCG CGGTATCGGA CGCGCGGTCG TCGAGGGACT GCTTGCCGAG GGCGCCGCGG TCGCGTTCTG TGCCCGCACT CCCGAGGCGG TCACCGCGAC GCAGCAGGAA CTCGCCGCCG ACGGCGTCAC CGTCGTCGGC ACCCCGGTCG ACGTCGGTGA CGGCGCCGCG GTCGCCGACT GGGTCGCGCA GAGCGCCGAG ACGCTCGGCG GCCTCGACGG CGTGGTGGCC AACGTGAGTG CGCTGGCCAT CCCCGAGAGC CCCGAGAACT GGCGCACCAG TTTCGAGGTG GACATGATGG GCACCGTCGG GCTGGTCGAC GCGGCCCTGC CGCACCTTCT GAAAAGTGAC GCGGGATCCA TCGTGACGAT CTCGAGCGTG TCGGGCCGCG AGATCGACTT CGCGGCCGGG CCGTACGGCA CGTTCAAGGC CGCGATCATC CACTACACCC AGGGCCTGGC GTACCAGCTC GCGGCGAAGG GCGTCCGCGC CAACTCGGTC AGCCCGGGCA ACACCTACTT CGAGGGCGGC GTGTGGCCCA CCATCGAGAA GGACGACCCC GAGTTGTTTG CTTCTGCCCT CGCGCTCAAC CCCACCGGGC GGATGGGGAC GCCACGTGAG GTTGCCAACG CCGTCGTGTT CCTCAGCAGC CCGGTCGCGA GCTTCATCAC CGGAACGAAC CTGGTGGTCG ACGGTGCACT GACCCGGGGC GTGCAGTTC
Details for MysmA.01365.c.A1.GE28917
HARVESTED ON: 3/30/2010
SEQUENCED ON: 4/20/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Validated AA Sequence
MDLGLTGKRF VVTGGTRGIG RAVVEGLLAE GAAVAFCART PEAVTATQQE LAADGVTVVG TPVDVGDGAA VADWVAQSAE TLGGLDGVVA NVSALAIPES PENWRTSFEV DMMGTVGLVD AALPHLLKSD AGSIVTISSV SGREIDFAAG PYGTFKAAII HYTQGLAYQL AAKGVRANSV SPGNTYFEGG VWPTIEKDDP ELFASALALN PTGRMGTPRE VANAVVFLSS PVASFITGTN LVVDGALTRG
Validated NT Sequence
cacaccccgg gtcagtgcac cgtcgaccac caggttcgtt ccggtgatga agctcgcgac cgggctgctg aggaacacga cggcgttggc aacctcacgt ggcgtcccca tccgcccggt ggggttgagc gcgagggcag aagcaaacaa ctcggggtcg tccttctcga tggtgggcca cacgccgccc tcgaagtagg tgttgcccgg gctgaccgag ttggcgcgga cgcccttcgc cgcgagctgg tacgccaggc cctgggtgta gtggatgatc gcggccttga acgtgccgta cggcccggcc gcgaagtcga tctcgcggcc cgacacgctc gagatcgtca cgatggatcc cgcgtcactt ttcagaaggt gcggcagggc cgcgtcgacc agcccgacgg tgcccatcat gtccacctcg aaactggtgc gccagttctc ggggctctcg gggatggcca gcgcactcac gttggccacc acgccgtcga ggccgccgag cgtctcggcg ctctgcgcga cccagtcggc gaccgcggcg ccgtcaccga cgtcgaccgg ggtgccgacg acggtgacgc cgtcggcggc gagttcctgc tgcgtcgcgg tgaccgcctc gggagtgcgg gcacagaacg cgaccgcggc gccctcggca agcagtccct cgacgaccgc gcgtccgata ccgcgcgtgc cgcccgtgac cacgaagcgt ttaccggtca gtccaagatc catcgaacca ggaccctggg tctgagcttc c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLGLTGKR FVVTGGTRGI GRAVVEGLLA EGAAVAFCAR TPEAVTATQQ ELAADGVTVV GTPVDVGDGA AVADWVAQSA ETLGGLDGVV ANVSALAIPE SPENWRTSFE VDMMGTVGLV DAALPHLLKS DAGSIVTISS VSGREIDFAA GPYGTFKAAI IHYTQGLAYQ LAAKGVRANS VSPGNTYFEG GVWPTIEKDD PELFASALAL NPTGRMGTPR EVANAVVFLS SPVASFITGT NLVVDGALTR GVQF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcttgga ctgaccggta aacgcttcgt ggtcacgggc ggcacgcgcg gtatcggacg cgcggtcgtc gagggactgc ttgccgaggg cgccgcggtc gcgttctgtg cccgcactcc cgaggcggtc accgcgacgc agcaggaact cgccgccgac ggcgtcaccg tcgtcggcac cccggtcgac gtcggtgacg gcgccgcggt cgccgactgg gtcgcgcaga gcgccgagac gctcggcggc ctcgacggcg tggtggccaa cgtgagtgcg ctggccatcc ccgagagccc cgagaactgg cgcaccagtt tcgaggtgga catgatgggc accgtcgggc tggtcgacgc ggccctgccg caccttctga aaagtgacgc gggatccatc gtgacgatct cgagcgtgtc gggccgcgag atcgacttcg cggccgggcc gtacggcacg ttcaaggccg cgatcatcca ctacacccag ggcctggcgt accagctcgc ggcgaagggc gtccgcgcca actcggtcag cccgggcaac acctacttcg agggcggcgt gtggcccacc atcgagaagg acgaccccga gttgtttgct tctgccctcg cgctcaaccc caccgggcgg atggggacgc cacgtgaggt tgccaacgcc gtcgtgttcc tcagcagccc ggtcgcgagc ttcatcaccg gaacgaacct ggtggtcgac ggtgcactga cccggggcgt gcagttcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MysmA.01365.c.B1.GE43087
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/25/2019
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD LGLTGKRFVV TGGTRGIGRA VVEGLLAEGA AVAFCARTPE AVTATQQELA ADGVTVVGTP VDVGDGAAVA DWVAQSAETL GGLDGVVANV SALAIPESPE NWRTSFEVDM MGTVGLVDAA LPHLLKSDAG SIVTISSVSG REIDFAAGPY GTFKAAIIHY TQGLAYQLAA KGVRANSVSP GNTYFEGGVW PTIEKDDPEL FASALALNPT GRMGTPREVA NAVVFLSSPV ASFITGTNLV VXXAXTRGVQ F
Validated NT Sequence
atcttactca cttagaactg cacgccccgn gtcnnngcag nnnnnaccac caggttcgtt ccggtgatga agctcgcgac cgggctgctg aggaacacga cggcgttggc aacctcacgt ggcgtcccca tccgcccggt ggggttgagc gcgagggcag aagcaaacaa ctcggggtcg tccttctcga tggtgggcca cacgccgccc tcgaagtagg tgttgcccgg gctgaccgag ttggcgcgga cgcccttcgc cgcgagctgg tacgccaggc cctgggtgta gtggatgatc gcggccttga acgtgccgta cggcccggcc gcgaagtcga tctcgcggcc cgacacgctc gagatcgtca cgatggatcc cgcgtcactt ttcagaaggt gcggcagggc cgcgtcgacc agcccgacgg tgcccatcat gtccacctcg aaactggtgc gccagttctc ggggctctcg gggatggcca gcgcactcac gttggccacc acgccgtcga ggccgccgag cgtctcggcg ctctgcgcga cccagtcggc gaccgcggcg ccgtcaccga cgtcgaccgg ggtgccgacg acggtgacgc cgtcggcggc gagttcctgc tgcgtcgcgg tgaccgcctc gggagtgcgg gcacagaacg cgaccgcggc gccctcggca agcagtccct cgacgaccgc gcgtccgata ccgcgcgtgc cgcccgtgac cacgaagcgt ttaccggtca gtccaagatc catatggtgg tggtggtggt gagccatg
Expected Protein Sequence
MAHHHHHHMD LGLTGKRFVV TGGTRGIGRA VVEGLLAEGA AVAFCARTPE AVTATQQELA ADGVTVVGTP VDVGDGAAVA DWVAQSAETL GGLDGVVANV SALAIPESPE NWRTSFEVDM MGTVGLVDAA LPHLLKSDAG SIVTISSVSG REIDFAAGPY GTFKAAIIHY TQGLAYQLAA KGVRANSVSP GNTYFEGGVW PTIEKDDPEL FASALALNPT GRMGTPREVA NAVVFLSSPV ASFITGTNLV VDGALTRGVQ F
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc ttggactgac cggtaaacgc ttcgtggtca cgggcggcac gcgcggtatc ggacgcgcgg tcgtcgaggg actgcttgcc gagggcgccg cggtcgcgtt ctgtgcccgc actcccgagg cggtcaccgc gacgcagcag gaactcgccg ccgacggcgt caccgtcgtc ggcaccccgg tcgacgtcgg tgacggcgcc gcggtcgccg actgggtcgc gcagagcgcc gagacgctcg gcggcctcga cggcgtggtg gccaacgtga gtgcgctggc catccccgag agccccgaga actggcgcac cagtttcgag gtggacatga tgggcaccgt cgggctggtc gacgcggccc tgccgcacct tctgaaaagt gacgcgggat ccatcgtgac gatctcgagc gtgtcgggcc gcgagatcga cttcgcggcc gggccgtacg gcacgttcaa ggccgcgatc atccactaca cccagggcct ggcgtaccag ctcgcggcga agggcgtccg cgccaactcg gtcagcccgg gcaacaccta cttcgagggc ggcgtgtggc ccaccatcga gaaggacgac cccgagttgt ttgcttctgc cctcgcgctc aaccccaccg ggcggatggg gacgccacgt gaggttgcca acgccgtcgt gttcctcagc agcccggtcg cgagcttcat caccggaacg aacctggtgg tcgacggtgc actgacccgg ggcgtgcagt tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for MysmA.01365.c.A1.PW28822
PURIFICATION DATe: 7/5/2010
CONCENTRATION: 25.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MDLGLTGKRF VVTGGTRGIG RAVVEGLLAE GAAVAFCART PEAVTATQQE LAADGVTVVG TPVDVGDGAA VADWVAQSAE TLGGLDGVVA NVSALAIPES PENWRTSFEV DMMGTVGLVD AALPHLLKSD AGSIVTISSV SGREIDFAAG PYGTFKAAII HYTQGLAYQL AAKGVRANSV SPGNTYFEGG VWPTIEKDDP ELFASALALN PTGRMGTPRE VANAVVFLSS PVASFITGTN LVVDGALTRG
Validated NT Sequence
cacaccccgg gtcagtgcac cgtcgaccac caggttcgtt ccggtgatga agctcgcgac cgggctgctg aggaacacga cggcgttggc aacctcacgt ggcgtcccca tccgcccggt ggggttgagc gcgagggcag aagcaaacaa ctcggggtcg tccttctcga tggtgggcca cacgccgccc tcgaagtagg tgttgcccgg gctgaccgag ttggcgcgga cgcccttcgc cgcgagctgg tacgccaggc cctgggtgta gtggatgatc gcggccttga acgtgccgta cggcccggcc gcgaagtcga tctcgcggcc cgacacgctc gagatcgtca cgatggatcc cgcgtcactt ttcagaaggt gcggcagggc cgcgtcgacc agcccgacgg tgcccatcat gtccacctcg aaactggtgc gccagttctc ggggctctcg gggatggcca gcgcactcac gttggccacc acgccgtcga ggccgccgag cgtctcggcg ctctgcgcga cccagtcggc gaccgcggcg ccgtcaccga cgtcgaccgg ggtgccgacg acggtgacgc cgtcggcggc gagttcctgc tgcgtcgcgg tgaccgcctc gggagtgcgg gcacagaacg cgaccgcggc gccctcggca agcagtccct cgacgaccgc gcgtccgata ccgcgcgtgc cgcccgtgac cacgaagcgt ttaccggtca gtccaagatc catcgaacca ggaccctggg tctgagcttc c
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLGLTGKR FVVTGGTRGI GRAVVEGLLA EGAAVAFCAR TPEAVTATQQ ELAADGVTVV GTPVDVGDGA AVADWVAQSA ETLGGLDGVV ANVSALAIPE SPENWRTSFE VDMMGTVGLV DAALPHLLKS DAGSIVTISS VSGREIDFAA GPYGTFKAAI IHYTQGLAYQ LAAKGVRANS VSPGNTYFEG GVWPTIEKDD PELFASALAL NPTGRMGTPR EVANAVVFLS SPVASFITGT NLVVDGALTR GVQF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGATCTTGGA CTGACCGGTA AACGCTTCGT GGTCACGGGC GGCACGCGCG GTATCGGACG CGCGGTCGTC GAGGGACTGC TTGCCGAGGG CGCCGCGGTC GCGTTCTGTG CCCGCACTCC CGAGGCGGTC ACCGCGACGC AGCAGGAACT CGCCGCCGAC GGCGTCACCG TCGTCGGCAC CCCGGTCGAC GTCGGTGACG GCGCCGCGGT CGCCGACTGG GTCGCGCAGA GCGCCGAGAC GCTCGGCGGC CTCGACGGCG TGGTGGCCAA CGTGAGTGCG CTGGCCATCC CCGAGAGCCC CGAGAACTGG CGCACCAGTT TCGAGGTGGA CATGATGGGC ACCGTCGGGC TGGTCGACGC GGCCCTGCCG CACCTTCTGA AAAGTGACGC GGGATCCATC GTGACGATCT CGAGCGTGTC GGGCCGCGAG ATCGACTTCG CGGCCGGGCC GTACGGCACG TTCAAGGCCG CGATCATCCA CTACACCCAG GGCCTGGCGT ACCAGCTCGC GGCGAAGGGC GTCCGCGCCA ACTCGGTCAG CCCGGGCAAC ACCTACTTCG AGGGCGGCGT GTGGCCCACC ATCGAGAAGG ACGACCCCGA GTTGTTTGCT TCTGCCCTCG CGCTCAACCC CACCGGGCGG ATGGGGACGC CACGTGAGGT TGCCAACGCC GTCGTGTTCC TCAGCAGCCC GGTCGCGAGC TTCATCACCG GAACGAACCT GGTGGTCGAC GGTGCACTGA CCCGGGGCGT GCAGTTCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA