MysmA.01322.a

Succinyl-CoA:3-ketoacid-coenzyme A transferase 1,

CENTER ID: MysmA.01322.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01322.a.A1.GE28902 Full length( MysmA.01322.a ) 1 265
MysmA.01322.a.AE1.GE43164 Full length( MysmA.01322.a ) 1 265
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01322.a.A1.PS00684 Full length( MysmA.01322.a ) 1 265
MysmA.01322.a.AE1.PW38688 Full length( MysmA.01322.a ) 1 265

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1880
RefSeq: YP_886264.1
UniProt: A0QTM6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTIDKVVGSA AEAVADVPRG AAIAVGGFGL AGIPWFLIEA LLEQGADDLT IVSNNCGVDG AGLGLLLEAG RISRVIASYV GENKEFARQY LAGELTVELT PQGTLAERMR AGGSGIGAFF TPTGVGTLVA EGGLPWRYHP DGTVALASPA KEVRTFNGKD MVLEESIVTD FALVRAAVVD RAGNCKFHAA ARNFNPPAAM AGRITVAEAE KVVEVGELHP DEIHLPGIFV QRVVALTPEQ AAHKDIEKRT TRPRPVTEEP EEMTR
NT Sequence
ATGACCATCG ACAAGGTCGT CGGCTCGGCC GCCGAAGCCG TCGCCGACGT TCCCCGCGGT GCCGCCATCG CCGTCGGCGG ATTCGGATTG GCAGGCATCC CATGGTTTCT CATCGAGGCG CTGCTTGAGC AGGGTGCCGA CGACCTGACG ATCGTCTCCA ACAACTGCGG GGTCGACGGC GCGGGTCTGG GCCTGCTGCT CGAAGCGGGC CGCATCAGCC GCGTCATCGC GTCGTACGTG GGGGAGAACA AGGAGTTCGC CAGGCAGTAC CTGGCCGGTG AACTCACCGT CGAGTTGACG CCGCAAGGCA CACTGGCCGA GCGGATGCGC GCAGGCGGCA GCGGAATCGG TGCGTTCTTC ACCCCGACCG GGGTGGGGAC ACTGGTCGCC GAAGGCGGGC TCCCGTGGCG CTACCACCCG GACGGCACCG TGGCGCTGGC GTCACCGGCC AAGGAGGTCC GCACCTTCAA CGGCAAGGAC ATGGTGCTCG AGGAGTCGAT CGTCACCGAC TTCGCGCTCG TGCGTGCCGC GGTCGTCGAC AGGGCAGGCA ACTGCAAGTT CCACGCCGCC GCACGCAATT TCAACCCGCC CGCGGCCATG GCGGGCCGGA TCACGGTGGC CGAGGCCGAG AAGGTCGTCG AGGTCGGTGA ACTGCACCCC GATGAGATCC ACCTGCCCGG CATCTTCGTG CAGCGCGTCG TCGCGCTCAC CCCGGAACAG GCCGCGCACA AGGACATCGA GAAACGCACC ACCCGGCCGC GGCCGGTCAC CGAGGAGCCC GAGGAGATGA CCCGA
Details for MysmA.01322.a.A1.GE28902
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDKVVGS AAEAVADVPR GAAIAVGGFG LAGIPWFLIE ALLEQGADDL TIVSNNCGVD GAGLGLLLEA GRISRVIASY VGENKEFARQ YLAGELTVEL TPQGTLAERM RAGGSGIGAF FTPTGVGTLV AEGGLPWRYH PDGTVALASP AKEVRTFNGK DMVLEESIVT DFALVRAAVV DRAGNCKFHA AARNFNPPAA MAGRITVAEA EKVVEVGELH PDEIHLPGIF VQRVVALTPE QAAHKDIEKR TTRPRPVTEE PEEMTR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca tcgacaaggt cgtcggctcg gccgccgaag ccgtcgccga cgttccccgc ggtgccgcca tcgccgtcgg cggattcgga ttggcaggca tcccatggtt tctcatcgag gcgctgcttg agcagggtgc cgacgacctg acgatcgtct ccaacaactg cggggtcgac ggcgcgggtc tgggcctgct gctcgaagcg ggccgcatca gccgcgtcat cgcgtcgtac gtgggggaga acaaggagtt cgccaggcag tacctggccg gtgaactcac cgtcgagttg acgccgcaag gcacactggc cgagcggatg cgcgcaggcg gcagcggaat cggtgcgttc ttcaccccga ccggggtggg gacactggtc gccgaaggcg ggctcccgtg gcgctaccac ccggacggca ccgtggcgct ggcgtcaccg gccaaggagg tccgcacctt caacggcaag gacatggtgc tcgaggagtc gatcgtcacc gacttcgcgc tcgtgcgtgc cgcggtcgtc gacagggcag gcaactgcaa gttccacgcc gccgcacgca atttcaaccc gcccgcggcc atggcgggcc ggatcacggt ggccgaggcc gagaaggtcg tcgaggtcgg tgaactgcac cccgatgaga tccacctgcc cggcatcttc gtgcagcgcg tcgtcgcgct caccccggaa caggccgcgc acaaggacat cgagaaacgc accacccggc cgcggccggt caccgaggag cccgaggaga tgacccgata ataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDKVVGS AAEAVADVPR GAAIAVGGFG LAGIPWFLIE ALLEQGADDL TIVSNNCGVD GAGLGLLLEA GRISRVIASY VGENKEFARQ YLAGELTVEL TPQGTLAERM RAGGSGIGAF FTPTGVGTLV AEGGLPWRYH PDGTVALASP AKEVRTFNGK DMVLEESIVT DFALVRAAVV DRAGNCKFHA AARNFNPPAA MAGRITVAEA EKVVEVGELH PDEIHLPGIF VQRVVALTPE QAAHKDIEKR TTRPRPVTEE PEEMTR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccatcgac aaggtcgtcg gctcggccgc cgaagccgtc gccgacgttc cccgcggtgc cgccatcgcc gtcggcggat tcggattggc aggcatccca tggtttctca tcgaggcgct gcttgagcag ggtgccgacg acctgacgat cgtctccaac aactgcgggg tcgacggcgc gggtctgggc ctgctgctcg aagcgggccg catcagccgc gtcatcgcgt cgtacgtggg ggagaacaag gagttcgcca ggcagtacct ggccggtgaa ctcaccgtcg agttgacgcc gcaaggcaca ctggccgagc ggatgcgcgc aggcggcagc ggaatcggtg cgttcttcac cccgaccggg gtggggacac tggtcgccga aggcgggctc ccgtggcgct accacccgga cggcaccgtg gcgctggcgt caccggccaa ggaggtccgc accttcaacg gcaaggacat ggtgctcgag gagtcgatcg tcaccgactt cgcgctcgtg cgtgccgcgg tcgtcgacag ggcaggcaac tgcaagttcc acgccgccgc acgcaatttc aacccgcccg cggccatggc gggccggatc acggtggccg aggccgagaa ggtcgtcgag gtcggtgaac tgcaccccga tgagatccac ctgcccggca tcttcgtgca gcgcgtcgtc gcgctcaccc cggaacaggc cgcgcacaag gacatcgaga aacgcaccac ccggccgcgg ccggtcaccg aggagcccga ggagatgacc cgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.01322.a.AE1.GE43164
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MTIDKVVGSA AEAVADVPRG AAIAVGGFGL AGIPWFLIEA LLEQGADDLT IVSNNCGVDG AGLGLLLEAG RISRVIASYV GENKEFARQY LAGELTVELT PQGTLAERMR AGGSGIGAFF TPTGVGTLVA EGGLPWRYHP DGTVALASPA KEVRTFNGKD MVLEESIVTD FALVRAAVVD RAGNCKFHAA ARNFNPPAAM AGRITVAEAE KVVEVGELHP DEIHLPGIFV QRVVALTPEQ AAHKDIEKRT TRPRPVTEEP EEMTRAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctcgggtcat ctcctcgggc tcctcggtga ccggccgcgg ccgggtggtg cgtttctcga tgtccttgtg cgcggcctgt tccggggtga gcgcgacgac gcgctgcacg aagatgccgg gcaggtggat ctcatcgggg tgcagttcac cgacctcgac gaccttctcg gcctcggcca ccgtgatccg gcccgccatg gccgcgggcg ggttgaaatt gcgtgcggcg gcgtggaact tgcagttgcc tgccctgtcg acgaccgcgg cacgcacgag cgcgaagtcg gtgacgatcg actcctcgag caccatgtcc ttgccgttga aggtgcggac ctccttggcc ggtgacgcca gcgccacggt gccgtccggg tggtagcgcc acgggagccc gccttcggcg accagtgtcc ccaccccggt cggggtgaag aacgcaccga ttccgctgcc gcctgcgcgc atccgctcgg ccagtgtgcc ttgcggcgtc aactcgacgg tgagttcacc ggccaggtac tgcctggcga actccttgtt ctcccccacg tacgacgcga tgacgcggct gatgcggccc gcttcgagca gcaggcccag acccgcgccg tcgaccccgc agttgttgga gacgatcgtc aggtcgtcgg caccctgctc aagcagcgcc tcgatgagaa accatgggat gcctgccaat ccgaatccgc cgacggcgat ggcggcaccg cggggaacgt cggcgacggc ttcggcggcc gagccgacga ccttgtcgat ggtcatggga gaga
Expected Protein Sequence
MTIDKVVGSA AEAVADVPRG AAIAVGGFGL AGIPWFLIEA LLEQGADDLT IVSNNCGVDG AGLGLLLEAG RISRVIASYV GENKEFARQY LAGELTVELT PQGTLAERMR AGGSGIGAFF TPTGVGTLVA EGGLPWRYHP DGTVALASPA KEVRTFNGKD MVLEESIVTD FALVRAAVVD RAGNCKFHAA ARNFNPPAAM AGRITVAEAE KVVEVGELHP DEIHLPGIFV QRVVALTPEQ AAHKDIEKRT TRPRPVTEEP EEMTRGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat gaccatcgac aaggtcgtcg gctcggccgc cgaagccgtc gccgacgttc cccgcggtgc cgccatcgcc gtcggcggat tcggattggc aggcatccca tggtttctca tcgaggcgct gcttgagcag ggtgccgacg acctgacgat cgtctccaac aactgcgggg tcgacggcgc gggtctgggc ctgctgctcg aagcgggccg catcagccgc gtcatcgcgt cgtacgtggg ggagaacaag gagttcgcca ggcagtacct ggccggtgaa ctcaccgtcg agttgacgcc gcaaggcaca ctggccgagc ggatgcgcgc aggcggcagc ggaatcggtg cgttcttcac cccgaccggg gtggggacac tggtcgccga aggcgggctc ccgtggcgct accacccgga cggcaccgtg gcgctggcgt caccggccaa ggaggtccgc accttcaacg gcaaggacat ggtgctcgag gagtcgatcg tcaccgactt cgcgctcgtg cgtgccgcgg tcgtcgacag ggcaggcaac tgcaagttcc acgccgccgc acgcaatttc aacccgcccg cggccatggc gggccggatc acggtggccg aggccgagaa ggtcgtcgag gtcggtgaac tgcaccccga tgagatccac ctgcccggca tcttcgtgca gcgcgtcgtc gcgctcaccc cggaacaggc cgcgcacaag gacatcgaga aacgcaccac ccggccgcgg ccggtcaccg aggagcccga ggagatgacc cgagggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MysmA.01322.a.A1.PS00684
PURIFICATION DATe: 7/13/2010
CONCENTRATION: 58.77mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDKVVGS AAEAVADVPR GAAIAVGGFG LAGIPWFLIE ALLEQGADDL TIVSNNCGVD GAGLGLLLEA GRISRVIASY VGENKEFARQ YLAGELTVEL TPQGTLAERM RAGGSGIGAF FTPTGVGTLV AEGGLPWRYH PDGTVALASP AKEVRTFNGK DMVLEESIVT DFALVRAAVV DRAGNCKFHA AARNFNPPAA MAGRITVAEA EKVVEVGELH PDEIHLPGIF VQRVVALTPE QAAHKDIEKR TTRPRPVTEE PEEMTR
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca tcgacaaggt cgtcggctcg gccgccgaag ccgtcgccga cgttccccgc ggtgccgcca tcgccgtcgg cggattcgga ttggcaggca tcccatggtt tctcatcgag gcgctgcttg agcagggtgc cgacgacctg acgatcgtct ccaacaactg cggggtcgac ggcgcgggtc tgggcctgct gctcgaagcg ggccgcatca gccgcgtcat cgcgtcgtac gtgggggaga acaaggagtt cgccaggcag tacctggccg gtgaactcac cgtcgagttg acgccgcaag gcacactggc cgagcggatg cgcgcaggcg gcagcggaat cggtgcgttc ttcaccccga ccggggtggg gacactggtc gccgaaggcg ggctcccgtg gcgctaccac ccggacggca ccgtggcgct ggcgtcaccg gccaaggagg tccgcacctt caacggcaag gacatggtgc tcgaggagtc gatcgtcacc gacttcgcgc tcgtgcgtgc cgcggtcgtc gacagggcag gcaactgcaa gttccacgcc gccgcacgca atttcaaccc gcccgcggcc atggcgggcc ggatcacggt ggccgaggcc gagaaggtcg tcgaggtcgg tgaactgcac cccgatgaga tccacctgcc cggcatcttc gtgcagcgcg tcgtcgcgct caccccggaa caggccgcgc acaaggacat cgagaaacgc accacccggc cgcggccggt caccgaggag cccgaggaga tgacccgata ataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTIDKVVGS AAEAVADVPR GAAIAVGGFG LAGIPWFLIE ALLEQGADDL TIVSNNCGVD GAGLGLLLEA GRISRVIASY VGENKEFARQ YLAGELTVEL TPQGTLAERM RAGGSGIGAF FTPTGVGTLV AEGGLPWRYH PDGTVALASP AKEVRTFNGK DMVLEESIVT DFALVRAAVV DRAGNCKFHA AARNFNPPAA MAGRITVAEA EKVVEVGELH PDEIHLPGIF VQRVVALTPE QAAHKDIEKR TTRPRPVTEE PEEMTR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCATCGAC AAGGTCGTCG GCTCGGCCGC CGAAGCCGTC GCCGACGTTC CCCGCGGTGC CGCCATCGCC GTCGGCGGAT TCGGATTGGC AGGCATCCCA TGGTTTCTCA TCGAGGCGCT GCTTGAGCAG GGTGCCGACG ACCTGACGAT CGTCTCCAAC AACTGCGGGG TCGACGGCGC GGGTCTGGGC CTGCTGCTCG AAGCGGGCCG CATCAGCCGC GTCATCGCGT CGTACGTGGG GGAGAACAAG GAGTTCGCCA GGCAGTACCT GGCCGGTGAA CTCACCGTCG AGTTGACGCC GCAAGGCACA CTGGCCGAGC GGATGCGCGC AGGCGGCAGC GGAATCGGTG CGTTCTTCAC CCCGACCGGG GTGGGGACAC TGGTCGCCGA AGGCGGGCTC CCGTGGCGCT ACCACCCGGA CGGCACCGTG GCGCTGGCGT CACCGGCCAA GGAGGTCCGC ACCTTCAACG GCAAGGACAT GGTGCTCGAG GAGTCGATCG TCACCGACTT CGCGCTCGTG CGTGCCGCGG TCGTCGACAG GGCAGGCAAC TGCAAGTTCC ACGCCGCCGC ACGCAATTTC AACCCGCCCG CGGCCATGGC GGGCCGGATC ACGGTGGCCG AGGCCGAGAA GGTCGTCGAG GTCGGTGAAC TGCACCCCGA TGAGATCCAC CTGCCCGGCA TCTTCGTGCA GCGCGTCGTC GCGCTCACCC CGGAACAGGC CGCGCACAAG GACATCGAGA AACGCACCAC CCGGCCGCGG CCGGTCACCG AGGAGCCCGA GGAGATGACC CGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA
Details for MysmA.01322.a.AE1.PW38688
PURIFICATION DATe: 8/12/2019
CONCENTRATION: 14mg/ml
OBSERVED MW: 27kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MTIDKVVGSA AEAVADVPRG AAIAVGGFGL AGIPWFLIEA LLEQGADDLT IVSNNCGVDG AGLGLLLEAG RISRVIASYV GENKEFARQY LAGELTVELT PQGTLAERMR AGGSGIGAFF TPTGVGTLVA EGGLPWRYHP DGTVALASPA KEVRTFNGKD MVLEESIVTD FALVRAAVVD RAGNCKFHAA ARNFNPPAAM AGRITVAEAE KVVEVGELHP DEIHLPGIFV QRVVALTPEQ AAHKDIEKRT TRPRPVTEEP EEMTRAGHHH HHH
Validated NT Sequence
tccgttaatg atgatgatgg tggtgcccag ctcgggtcat ctcctcgggc tcctcggtga ccggccgcgg ccgggtggtg cgtttctcga tgtccttgtg cgcggcctgt tccggggtga gcgcgacgac gcgctgcacg aagatgccgg gcaggtggat ctcatcgggg tgcagttcac cgacctcgac gaccttctcg gcctcggcca ccgtgatccg gcccgccatg gccgcgggcg ggttgaaatt gcgtgcggcg gcgtggaact tgcagttgcc tgccctgtcg acgaccgcgg cacgcacgag cgcgaagtcg gtgacgatcg actcctcgag caccatgtcc ttgccgttga aggtgcggac ctccttggcc ggtgacgcca gcgccacggt gccgtccggg tggtagcgcc acgggagccc gccttcggcg accagtgtcc ccaccccggt cggggtgaag aacgcaccga ttccgctgcc gcctgcgcgc atccgctcgg ccagtgtgcc ttgcggcgtc aactcgacgg tgagttcacc ggccaggtac tgcctggcga actccttgtt ctcccccacg tacgacgcga tgacgcggct gatgcggccc gcttcgagca gcaggcccag acccgcgccg tcgaccccgc agttgttgga gacgatcgtc aggtcgtcgg caccctgctc aagcagcgcc tcgatgagaa accatgggat gcctgccaat ccgaatccgc cgacggcgat ggcggcaccg cggggaacgt cggcgacggc ttcggcggcc gagccgacga ccttgtcgat ggtcatggga gaga
Expressed Protein Sequence
MTIDKVVGSA AEAVADVPRG AAIAVGGFGL AGIPWFLIEA LLEQGADDLT IVSNNCGVDG AGLGLLLEAG RISRVIASYV GENKEFARQY LAGELTVELT PQGTLAERMR AGGSGIGAFF TPTGVGTLVA EGGLPWRYHP DGTVALASPA KEVRTFNGKD MVLEESIVTD FALVRAAVVD RAGNCKFHAA ARNFNPPAAM AGRITVAEAE KVVEVGELHP DEIHLPGIFV QRVVALTPEQ AAHKDIEKRT TRPRPVTEEP EEMTRGHHHH HH
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACATTAACGC TTACAATTTA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATTTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAG ATCCCGGTGC CTAATGAGTG AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC CAGTTGATCG GCGCGAGATT TAATCGCCGC GACAATTTGC GACGGCGCGT GCAGGGCCAG ACTGGAGGTG GCAACGCCAA TCAGCAACGA CTGTTTGCCC GCCAGTTGTT GTGCCACGCG GTTGGGAATG TAATTCAGCT CCGCCATCGC CGCTTCCACT TTTTCCCGCG TTTTCGCAGA AACGTGGCTG GCCTGGTTCA CCACGCGGGA AACGGTCTGA TAAGAGACAC CGGCATACTC TGCGACATCG TATAACGTTA CTGGTTTCAC ATTCACCACC CTGAATTGAC TCTCTTCCGG GCGCTATCAT GCCATACCGC GAAAGGTTTT GCGCCATTCG ATGGTGTCCG GGATCTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAATAATTT TGTTTAACTT TAAGAAGGAG TCTCTCCCAT GACCATCGAC AAGGTCGTCG GCTCGGCCGC CGAAGCCGTC GCCGACGTTC CCCGCGGTGC CGCCATCGCC GTCGGCGGAT TCGGATTGGC AGGCATCCCA TGGTTTCTCA TCGAGGCGCT GCTTGAGCAG GGTGCCGACG ACCTGACGAT CGTCTCCAAC AACTGCGGGG TCGACGGCGC GGGTCTGGGC CTGCTGCTCG AAGCGGGCCG CATCAGCCGC GTCATCGCGT CGTACGTGGG GGAGAACAAG GAGTTCGCCA GGCAGTACCT GGCCGGTGAA CTCACCGTCG AGTTGACGCC GCAAGGCACA CTGGCCGAGC GGATGCGCGC AGGCGGCAGC GGAATCGGTG CGTTCTTCAC CCCGACCGGG GTGGGGACAC TGGTCGCCGA AGGCGGGCTC CCGTGGCGCT ACCACCCGGA CGGCACCGTG GCGCTGGCGT CACCGGCCAA GGAGGTCCGC ACCTTCAACG GCAAGGACAT GGTGCTCGAG GAGTCGATCG TCACCGACTT CGCGCTCGTG CGTGCCGCGG TCGTCGACAG GGCAGGCAAC TGCAAGTTCC ACGCCGCCGC ACGCAATTTC AACCCGCCCG CGGCCATGGC GGGCCGGATC ACGGTGGCCG AGGCCGAGAA GGTCGTCGAG GTCGGTGAAC TGCACCCCGA TGAGATCCAC CTGCCCGGCA TCTTCGTGCA GCGCGTCGTC GCGCTCACCC CGGAACAGGC CGCGCACAAG GACATCGAGA AACGCACCAC CCGGCCGCGG CCGGTCACCG AGGAGCCCGA GGAGATGACC CGAGGGCACC ACCATCATCA TCATTAACGG ATCCGAATTC GAGCTCCGTC GACAAGCTTG CGGCCGCACT CGAGCACCAC CACCACCACC ACTGAGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA T