SSGCID: Target Page

MysmA.01285.a

Methylthioadenosine phosphorylase

CENTER ID:	MysmA.01285.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01285.a.A2.GE28901	Mature protein( MysmA.01285.a )		21	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.978
RefSeq:	YP_885392.1
UniProt:	A0QR54

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMLGVIGGSG FYTFFGSDAR AVSVETPYGP PSAPITVGTV GDHEVAFLPR HGVKHEFSPH TVPYRANLWA LRSLGVRRVF APCAVGSLTP DLGPGSIVVP DQLVDRTSGR DDTYFDSGGI HVAFADPYCP TLRAAATGLP GVVDGGTMVV IQGPRFSTRA ESRWFASQGF TLVNMTGYPE AVLARELEMC YAAVALVTDL DAGIEVGSGV RAVDVFAEFE RNMPPFKKLV FEALEAVEVE RTCTHCLTHS GVQLPFELP

NT Sequence

GTGATGCTCG GGGTCATCGG CGGCAGCGGT TTCTACACGT TCTTCGGCTC TGACGCGCGA GCGGTCAGCG TCGAGACCCC GTACGGCCCG CCGAGCGCGC CCATCACGGT GGGCACGGTG GGTGATCACG AGGTGGCGTT CCTGCCGCGG CACGGCGTGA AGCACGAGTT CTCGCCGCAC ACCGTGCCGT ACCGCGCCAA CCTGTGGGCG TTGCGCTCTC TCGGGGTGCG ACGCGTCTTC GCGCCATGCG CGGTCGGCAG CCTCACGCCG GATCTTGGGC CGGGTTCGAT CGTGGTGCCC GATCAGCTGG TCGACCGCAC CAGCGGGCGC GACGACACGT ACTTCGATTC CGGCGGAATC CATGTCGCCT TCGCCGACCC GTACTGCCCG ACGCTGCGCG CCGCGGCCAC GGGGCTTCCC GGCGTGGTCG ACGGTGGCAC CATGGTGGTG ATCCAGGGTC CGCGTTTTTC CACGCGCGCC GAGAGCCGCT GGTTCGCGAG CCAGGGCTTC ACGCTGGTGA ACATGACCGG CTATCCCGAG GCGGTCCTCG CTCGTGAGCT GGAGATGTGT TATGCGGCAG TCGCTTTGGT GACCGATCTG GACGCGGGCA TCGAGGTCGG GTCGGGTGTC CGCGCGGTCG ACGTGTTCGC CGAGTTCGAG CGCAACATGC CGCCGTTCAA GAAACTCGTC TTCGAGGCAC TCGAGGCCGT CGAGGTCGAG CGGACCTGCA CGCACTGCCT GACCCACAGC GGGGTGCAGT TGCCCTTCGA GTTGCCA

Details for MysmA.01285.a.A2.GE28901

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	106

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAVSVETPY GPPSAPITVG TVGDHEVAFL PRHGVKHEFS PHTVPYRANL WALRSLGVRR VFAPCAVGSL TPDLGPGSIV VPDQLVDRTS GRDDTYFDSG GIHVAFADPY CPTLRAAATG LPGVVDGGTM VVIQGPRFST RAESRWFASQ GFTLVNMTGY PEAVLARELE MCYAAVALVT DLDAGIEVGS GVRAVDVFAE FERNMPPFKK LLRGTRGRRG RADLHALPDP QRGAXALRVA IIXSTNKFCS QASRGSGC

Validated NT Sequence

ttnnnnnnnn tttaagaagg agaataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcggtc agcgtcgaga ccccgtacgg cccgccgagc gcgcccatca cggtgggcac ggtgggtgat cacgaggtgg cgttcctgcc gcggcacggc gtgaagcacg agttctcgcc gcacaccgtg ccgtaccgcg ccaacctgtg ggcgttgcgc tctctcgggg tgcgacgcgt cttcgcgcca tgcgcggtcg gcagcctcac gccggatctt gggccgggtt cgatcgtggt gcccgatcag ctggtcgacc gcaccagcgg gcgcgacgac acgtacttcg attccggcgg aatccatgtc gccttcgccg acccgtactg cccgacgctg cgcgccgcgg ccacggggct tcccggcgtg gtcgacggtg gcaccatggt ggtgatccag ggtccgcgtt tttccacgcg cgccgagagc cgctggttcg cgagccaggg cttcacgctg gtgaacatga ccggctatcc cgaggcggtc ctcgctcgtg agctggagat gtgttatgcg gcagtcgctt tggtgaccga tctggacgcg ggcatcgagg tcgggtcggg tgtccgcgcg gtcgacgtgt tcgccgagtt cgagcgcaac atgccgccgt tcaagaaact ccttcgaggc actcgaggcc gtcgaggtcg agcggacctg cacgcactgc ctgacccaca gcggggngca gntgcncttc gagttgccat aataaanagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacnaag cccgaaagnn agctganntg gctgctgcnn ccgctgannn nntanctanc a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtcagc gtcgagaccc cgtacggccc gccgagcgcg cccatcacgg tgggcacggt gggtgatcac gaggtggcgt tcctgccgcg gcacggcgtg aagcacgagt tctcgccgca caccgtgccg taccgcgcca acctgtgggc gttgcgctct ctcggggtgc gacgcgtctt cgcgccatgc gcggtcggca gcctcacgcc ggatcttggg ccgggttcga tcgtggtgcc cgatcagctg gtcgaccgca ccagcgggcg cgacgacacg tacttcgatt ccggcggaat ccatgtcgcc ttcgccgacc cgtactgccc gacgctgcgc gccgcggcca cggggcttcc cggcgtggtc gacggtggca ccatggtggt gatccagggt ccgcgttttt ccacgcgcgc cgagagccgc tggttcgcga gccagggctt cacgctggtg aacatgaccg gctatcccga ggcggtcctc gctcgtgagc tggagatgtg ttatgcggca gtcgctttgg tgaccgatct ggacgcgggc atcgaggtcg ggtcgggtgt ccgcgcggtc gacgtgttcg ccgagttcga gcgcaacatg ccgccgttca agaaactcgt cttcgaggca ctcgaggccg tcgaggtcga gcggacctgc acgcactgcc tgacccacag cggggtgcag ttgcccttcg agttgccaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa