MysmA.01263.a

Acyl-CoA dehydrogenase

CENTER ID: MysmA.01263.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01263.a.A1.PW28802 Full length( MysmA.01263.a ) 1 387

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4604
RefSeq: YP_888975.1
UniProt: A0R1D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDFLSTGSL PDDYEQLAKT VRDFAQSVVA PVAAKHDEEH SFPYEVVAGM ADMGLFGLPF PEEYGGMGGD YFALCLALEE LGRVDQSVAI TLEAGVSLGA MPVYRFGNDA QKQEWLPLLA SGKALGAFGL TEAGGGSDAG ATKTTARLDD GHWVINGSKQ FITNSGTDIT RLVTVTAVTG EKPDGRKEIS SILVPVPTEG FTAEPAYNKV GWNASDTHPL SFDDVRVPEE NLLGERGRGY ANFLRILDEG RIAIAALSVG AAQGCVDESV KYAKERQAFG AAIGTYQAIA FKIARMEARA HAARTAYYDA AALMLAGKPF KKAASVAKLV ASEAAMDNAR DATQIFGGYG FMNEYSVARH YRDSKILEIG EGTTEVQLML IGRELGL
NT Sequence
ATGACCGACT TCCTCTCGAC CGGGAGCCTC CCGGACGACT ACGAGCAACT CGCCAAGACC GTGCGGGACT TCGCCCAGAG CGTCGTGGCG CCGGTGGCCG CCAAACACGA CGAGGAGCAC TCGTTCCCGT ACGAGGTCGT GGCGGGCATG GCCGACATGG GCCTGTTCGG TCTGCCGTTC CCCGAGGAGT ACGGCGGCAT GGGCGGCGAC TACTTCGCGC TGTGCCTCGC GCTGGAGGAA CTCGGCCGCG TGGACCAGAG TGTCGCCATC ACTCTGGAGG CCGGAGTGTC GCTGGGCGCG ATGCCGGTGT ACCGGTTCGG CAACGACGCG CAGAAGCAGG AGTGGCTGCC GCTCTTGGCC AGTGGCAAGG CTCTGGGCGC GTTCGGCCTC ACCGAGGCAG GCGGCGGCAG CGACGCCGGT GCCACCAAGA CCACCGCGCG TCTCGACGAC GGGCACTGGG TGATCAACGG GTCCAAGCAG TTCATCACCA ACTCGGGCAC CGACATCACC CGCCTGGTCA CCGTCACCGC GGTGACAGGT GAGAAACCCG ATGGCCGCAA AGAGATCTCG TCGATCCTGG TGCCCGTGCC CACCGAGGGG TTCACGGCCG AACCGGCCTA CAACAAGGTC GGCTGGAACG CGTCGGACAC CCACCCGCTG AGCTTCGACG ACGTGCGCGT GCCCGAGGAG AACCTGCTCG GTGAGCGCGG CCGCGGCTAC GCCAACTTCC TGCGCATCCT CGATGAGGGC CGCATCGCGA TCGCGGCGCT GTCGGTCGGC GCGGCGCAGG GTTGCGTCGA CGAATCGGTG AAGTACGCCA AGGAACGTCA GGCATTCGGC GCGGCCATCG GCACCTACCA GGCGATCGCG TTCAAGATCG CCCGGATGGA GGCCCGCGCA CACGCCGCCC GCACGGCCTA CTACGACGCC GCGGCCCTCA TGCTGGCAGG CAAGCCGTTC AAGAAGGCCG CCTCGGTGGC CAAGCTGGTG GCCAGTGAGG CCGCGATGGA CAACGCACGC GACGCCACGC AGATCTTCGG TGGCTACGGG TTCATGAACG AGTACTCGGT GGCCCGGCAC TACCGCGACA GCAAGATCCT CGAAATCGGG GAAGGCACAA CGGAAGTTCA GCTGATGCTG ATCGGAAGGG AGCTCGGTCT G
Details for MysmA.01263.a.A1.PW28802
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 27.91mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 22
Protocol Notes
notes unavailable
Validated AA Sequence
MEARAHAART AYYDAAALML AGKPFKKAAS VAKLVASEAA MDNARDATQI FGGYGFMNEY SVARHYRDSK ILEIGEGTTE VQLMLIGREL G
Validated NT Sequence
agaccgagct cccttccgat cagcatcagc tgaacttccg ttgtgccttc cccgatttcg aggatcttgc tgtcgcggta gtgccgggcc accgagtact cgttcatgaa cccgtagcca ccgaagatct gcgtggcgtc gcgtgcgttg tccatcgcgg cctcactggc caccagcttg gccaccgagg cggccttctt gaacggcttg cctgccagca tgagggccgc ggcgtcgtag taggccgtgc gggcggcgtg tgcgcgggcc tccatccggg cgatcttgaa cgcgatcgcc tggtaggtgc cgatggccgc gccgaatgcc tgacgttcct tggcgtactt caccgattcg tcgacgcaac cctgcgccgc gccgaccgac agcgccgcga tcgcgatgcg gccctcatcg aggatgcgca ggaagttggc gtagccgcgg ccgcgctcac cgagcaggtt ctcctcgggc acgcgcacgt cgtcgaagct cagcgggtgg gtgtccgacg cgttccagcc gaccttgttg taggccggtt cggccgtgaa cccctcggtg ggcacgggca ccaggatcga cgagatctct ttgcggccat cgggtttctc acctgtcacc gcggtgacgg tgaccaggcg ggtgatgtcg gtgcccgagt tggtgatgaa ctgcttggac ccgttgatca cccagtgccc gtcgtcnaga cgcgcggtgg tcttggtggc accggcgtcg ctgccgccgc ctgcctcggt gaggccgaac gcgcccagag ccttgccact ggncaagagc ggcagccact cctgcttctg cgcgtcgttg ccnaancngg tacaccggcn tcgcgcccag cgacnctccn gncctccana gtgatggcna nnctctggtc cacg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDFLSTGS LPDDYEQLAK TVRDFAQSVV APVAAKHDEE HSFPYEVVAG MADMGLFGLP FPEEYGGMGG DYFALCLALE ELGRVDQSVA ITLEAGVSLG AMPVYRFGND AQKQEWLPLL ASGKALGAFG LTEAGGGSDA GATKTTARLD DGHWVINGSK QFITNSGTDI TRLVTVTAVT GEKPDGRKEI SSILVPVPTE GFTAEPAYNK VGWNASDTHP LSFDDVRVPE ENLLGERGRG YANFLRILDE GRIAIAALSV GAAQGCVDES VKYAKERQAF GAAIGTYQAI AFKIARMEAR AHAARTAYYD AAALMLAGKP FKKAASVAKL VASEAAMDNA RDATQIFGGY GFMNEYSVAR HYRDSKILEI GEGTTEVQLM LIGRELGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGACTTC CTCTCGACCG GGAGCCTCCC GGACGACTAC GAGCAACTCG CCAAGACCGT GCGGGACTTC GCCCAGAGCG TCGTGGCGCC GGTGGCCGCC AAACACGACG AGGAGCACTC GTTCCCGTAC GAGGTCGTGG CGGGCATGGC CGACATGGGC CTGTTCGGTC TGCCGTTCCC CGAGGAGTAC GGCGGCATGG GCGGCGACTA CTTCGCGCTG TGCCTCGCGC TGGAGGAACT CGGCCGCGTG GACCAGAGTG TCGCCATCAC TCTGGAGGCC GGAGTGTCGC TGGGCGCGAT GCCGGTGTAC CGGTTCGGCA ACGACGCGCA GAAGCAGGAG TGGCTGCCGC TCTTGGCCAG TGGCAAGGCT CTGGGCGCGT TCGGCCTCAC CGAGGCAGGC GGCGGCAGCG ACGCCGGTGC CACCAAGACC ACCGCGCGTC TCGACGACGG GCACTGGGTG ATCAACGGGT CCAAGCAGTT CATCACCAAC TCGGGCACCG ACATCACCCG CCTGGTCACC GTCACCGCGG TGACAGGTGA GAAACCCGAT GGCCGCAAAG AGATCTCGTC GATCCTGGTG CCCGTGCCCA CCGAGGGGTT CACGGCCGAA CCGGCCTACA ACAAGGTCGG CTGGAACGCG TCGGACACCC ACCCGCTGAG CTTCGACGAC GTGCGCGTGC CCGAGGAGAA CCTGCTCGGT GAGCGCGGCC GCGGCTACGC CAACTTCCTG CGCATCCTCG ATGAGGGCCG CATCGCGATC GCGGCGCTGT CGGTCGGCGC GGCGCAGGGT TGCGTCGACG AATCGGTGAA GTACGCCAAG GAACGTCAGG CATTCGGCGC GGCCATCGGC ACCTACCAGG CGATCGCGTT CAAGATCGCC CGGATGGAGG CCCGCGCACA CGCCGCCCGC ACGGCCTACT ACGACGCCGC GGCCCTCATG CTGGCAGGCA AGCCGTTCAA GAAGGCCGCC TCGGTGGCCA AGCTGGTGGC CAGTGAGGCC GCGATGGACA ACGCACGCGA CGCCACGCAG ATCTTCGGTG GCTACGGGTT CATGAACGAG TACTCGGTGG CCCGGCACTA CCGCGACAGC AAGATCCTCG AAATCGGGGA AGGCACAACG GAAGTTCAGC TGATGCTGAT CGGAAGGGAG CTCGGTCTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A