MysmA.01249.b

Short chain dehydrogenase

CENTER ID: MysmA.01249.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01249.b.A1.PW28799 Full length( MysmA.01249.b ) 1 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.854
RefSeq: YP_885266.1
UniProt: A0QQS8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSADTKWTEA DVPDQSGRVA IVTGSNTGLG YETARALAAK GAHVVIAVRN LDKGRDAVDR IMASTPKADL KLQKLDVGSL DSVRTAADEL KGAYPHIDLL INNAGVMYPP KQTTVDGFEL QFGTNHLGPF ALTGLLIDHL LPVEGSRVVA VASVAHRIRA KIHFEDLQWE RRYNRVEAYG QSKLANLLFA YELQRRLAAA GKPTISVAAH PGLSNTELMR HIPGTGLPGY HQIASLFSNS PLMGALATLR AATDPGVKGG QYYGPDGFRE VRGHPELVKS SSQSRDPELQ RRLWAVSEEL TGVSYPV
NT Sequence
ATGAGCGCCG ACACGAAGTG GACCGAGGCC GACGTTCCCG ATCAATCAGG CCGCGTGGCC ATCGTCACCG GATCCAACAC CGGACTGGGT TACGAGACCG CGAGGGCGCT GGCCGCCAAG GGCGCGCACG TGGTCATCGC GGTGCGCAAC CTGGACAAGG GCCGCGATGC CGTGGACCGG ATCATGGCCT CGACACCCAA GGCCGATCTG AAGCTGCAGA AGCTGGACGT GGGGTCACTG GATTCCGTGC GCACCGCGGC CGACGAACTC AAGGGCGCCT ACCCGCACAT CGATCTGCTG ATCAACAACG CCGGCGTGAT GTATCCGCCA AAGCAGACCA CCGTCGACGG GTTCGAGCTG CAGTTCGGCA CCAACCATCT GGGGCCGTTC GCGCTGACGG GTCTGCTCAT CGACCACCTG CTGCCGGTCG AGGGCTCACG CGTGGTGGCC GTCGCCAGCG TGGCCCACCG CATCCGCGCC AAGATCCACT TCGAGGATCT GCAGTGGGAA CGCCGCTACA ACCGGGTCGA GGCGTACGGC CAGTCCAAGC TCGCGAATCT GTTGTTCGCC TACGAACTTC AGCGTCGCCT CGCCGCGGCA GGCAAGCCGA CCATCTCGGT GGCCGCCCAT CCCGGGTTGT CGAACACCGA ACTCATGCGG CACATCCCCG GCACCGGGCT GCCCGGCTAC CACCAGATCG CGAGCCTGTT CTCCAACAGT CCGCTGATGG GCGCGCTGGC GACGCTGCGC GCGGCCACCG ATCCCGGTGT CAAGGGCGGC CAGTACTACG GCCCGGACGG GTTCCGTGAG GTGCGTGGAC ACCCCGAGCT CGTGAAATCC AGTTCCCAGT CCCGTGATCC GGAGTTGCAA CGCCGGTTGT GGGCCGTCTC AGAAGAGCTG ACCGGCGTCA GCTACCCGGT G
Details for MysmA.01249.b.A1.PW28799
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 26.51mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 100µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MSADTKWTEA DVPDQSGRVA XVTGSNTGLG YETARALAAK GAHVVIAVRN LDKGRDAVDR IMASTXKADL KLQKLDVGSL DSVRTAADEL KGAYPHIDLL INNAGVMYPP KQTTVDGFEL QFGTNHLGPF ALTGLLIDHL LPVEGSRVVA VASVAHRIRA KIHFEDLQWE RRYNRVEAYG QSKLANLLFA YELQRRLAAA GKPTISVAAH PGLSNTELMR HIPGTGLPGY HQIASLFSNS PLMGALATLR AATDPGVKGG QYYGPDGFRE VRGHPELVKS SSQSRDPELQ RRLWAVSEEL TGVSYPV
Validated NT Sequence
tcnttcgngc tttgttagca gccggatcct cgagaagctt ggctgcagaa cttgttcgtg ctgtttatta caccgggtag ctgacgccgg tcagctcttc tgagacggcc cacaaccggc gttgcaactc cggatcacgg gactgggaac tggatttcac gagctcgggg tgtccacgca cctcacggaa cccgtccggg ccgtagtact ggccgccctt gacaccggga tcggtggccg cgcgcagcgt cgccagcgcg cccatcagcg gactgttgga gaacaggctc gcgatctggt ggtagccggg cagcccggtg ccggggatgt gccgcatgag ttcggtgttc gacaacccgg gatgggcggc caccgagatg gtcggcttgc ctgccgcggc gaggcgacgc tgaagttcgt aggcgaacaa cagattcgcg agcttggact ggccgtacgc ctcgacccgg ttgtagcggc gttcccactg cagatcctcg aagtggatct tggcgcggat gcggtgggcc acgctggcga cggccaccac gcgtgagccc tcgaccggca gcaggtggtc gatgagcaga cccgtcagcg cgaacggccc cagatggttg gtgccgaact gcagctcgaa cccgtcgacg gtggtctgct ttggcggata catcacgccg gcgttgttga tcagcagatc gatgtgcggg taggcgccct tgagttcgtc ggccgcggtg cgcacggaat ccagtgaccc cacgtccagc ttctgcagct tcagatcggc cttgngtgtc gaggccatga tccggtccac ggcatcgcgg cccttgtcca ggttgcgcac cgcgatgacc acgtgcgcgc ccttggcggc cagcgccctc gcggtctcgt aacccagtcc ggtgttggat ccggtgacna tggccacgcg gcctgattga tcgggaacgt cngcctcggt ccacttcgtg tcggcgctca tcgaaccngg accctgggtc tgancttncn ggnacccata tgatnnnann atganncatg gnntatcnnc nnnnnanntn ancnaaatnn nnntaggnan cnnnnnncnn nnnnnnnnnn nnnnnnnnnn nnnnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSADTKWTE ADVPDQSGRV AIVTGSNTGL GYETARALAA KGAHVVIAVR NLDKGRDAVD RIMASTPKAD LKLQKLDVGS LDSVRTAADE LKGAYPHIDL LINNAGVMYP PKQTTVDGFE LQFGTNHLGP FALTGLLIDH LLPVEGSRVV AVASVAHRIR AKIHFEDLQW ERRYNRVEAY GQSKLANLLF AYELQRRLAA AGKPTISVAA HPGLSNTELM RHIPGTGLPG YHQIASLFSN SPLMGALATL RAATDPGVKG GQYYGPDGFR EVRGHPELVK SSSQSRDPEL QRRLWAVSEE LTGVSYPV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGCCGAC ACGAAGTGGA CCGAGGCCGA CGTTCCCGAT CAATCAGGCC GCGTGGCCAT CGTCACCGGA TCCAACACCG GACTGGGTTA CGAGACCGCG AGGGCGCTGG CCGCCAAGGG CGCGCACGTG GTCATCGCGG TGCGCAACCT GGACAAGGGC CGCGATGCCG TGGACCGGAT CATGGCCTCG ACACCCAAGG CCGATCTGAA GCTGCAGAAG CTGGACGTGG GGTCACTGGA TTCCGTGCGC ACCGCGGCCG ACGAACTCAA GGGCGCCTAC CCGCACATCG ATCTGCTGAT CAACAACGCC GGCGTGATGT ATCCGCCAAA GCAGACCACC GTCGACGGGT TCGAGCTGCA GTTCGGCACC AACCATCTGG GGCCGTTCGC GCTGACGGGT CTGCTCATCG ACCACCTGCT GCCGGTCGAG GGCTCACGCG TGGTGGCCGT CGCCAGCGTG GCCCACCGCA TCCGCGCCAA GATCCACTTC GAGGATCTGC AGTGGGAACG CCGCTACAAC CGGGTCGAGG CGTACGGCCA GTCCAAGCTC GCGAATCTGT TGTTCGCCTA CGAACTTCAG CGTCGCCTCG CCGCGGCAGG CAAGCCGACC ATCTCGGTGG CCGCCCATCC CGGGTTGTCG AACACCGAAC TCATGCGGCA CATCCCCGGC ACCGGGCTGC CCGGCTACCA CCAGATCGCG AGCCTGTTCT CCAACAGTCC GCTGATGGGC GCGCTGGCGA CGCTGCGCGC GGCCACCGAT CCCGGTGTCA AGGGCGGCCA GTACTACGGC CCGGACGGGT TCCGTGAGGT GCGTGGACAC CCCGAGCTCG TGAAATCCAG TTCCCAGTCC CGTGATCCGG AGTTGCAACG CCGGTTGTGG GCCGTCTCAG AAGAGCTGAC CGGCGTCAGC TACCCGGTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A