SSGCID: Target Page

MysmA.01204.a

Oxidoreductase, short chain dehydrogenase/reductase family protein

CENTER ID:	MysmA.01204.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01204.a.A2.GE28890	Mature protein( MysmA.01204.a )		24	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5403
RefSeq:	YP_889781.1
UniProt:	A0R3P3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTRQNILITG ASSGLGAGMA RQFAARGRNL ALCARRTDRL EELKAEIADR HPYIKVAIAA LDVNDHEAVP RVFNELSEEL GGIDRVIVNA GIGKGWPLGE GKPWANKATL ETNLVAGLVQ IEAALEMFKK AGGGHLVLIS SVLGNTGVPG GKAAYCASKA GMTSLGESLR AEYDKGPIRV TVVEPGYIES EMTAKANSTM LMVDNETGVR AMVEAIEKEK GRAVVPRWPW APLTQVMRLL PPKYTKRFA

NT Sequence

GTGACCCGTC AGAACATCCT CATCACCGGT GCCAGCTCAG GCCTGGGCGC CGGTATGGCC CGTCAATTCG CAGCCAGAGG CCGCAACCTG GCGCTGTGCG CGCGCCGCAC GGATCGTCTG GAGGAGCTCA AGGCGGAGAT CGCCGACCGG CACCCGTACA TCAAGGTGGC CATCGCCGCG CTGGACGTCA ACGATCACGA GGCCGTGCCC CGGGTGTTCA ACGAACTGTC CGAGGAACTC GGCGGTATCG ACCGCGTCAT CGTCAACGCG GGCATCGGCA AGGGCTGGCC GCTGGGCGAG GGCAAGCCGT GGGCCAACAA GGCCACGCTC GAGACCAACC TGGTCGCCGG TCTGGTGCAG ATCGAGGCCG CACTGGAGAT GTTCAAGAAG GCAGGCGGTG GACACCTGGT GCTGATCTCC TCGGTGCTGG GCAACACGGG TGTCCCCGGC GGCAAGGCCG CCTACTGTGC GAGCAAGGCC GGTATGACGT CGCTGGGCGA GTCCCTGCGC GCGGAGTACG ACAAGGGCCC CATCAGGGTC ACGGTCGTCG AACCGGGGTA CATCGAATCC GAGATGACGG CCAAGGCCAA CTCGACGATG CTCATGGTCG ACAACGAGAC CGGCGTGCGC GCCATGGTCG AGGCCATCGA GAAGGAGAAG GGGCGCGCCG TCGTGCCGCG CTGGCCGTGG GCGCCGCTGA CCCAGGTCAT GCGGCTGTTG CCACCTAAGT ACACCAAGCG CTTCGCA

Details for MysmA.01204.a.A2.GE28890

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	26kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAARGRNLA LCARRTDRLE ELKAEIADRH PYIKVAIAAL DVNDHEAVPR VFNELSEELG GIDRVIVNAG IGKGWPLGEG KPWANKATLE TNLVAGLVQI EAALEMFKKA GGGHLVLISS VLGNTGVPGG KAAYCASKAG MTSLGESLRA EYDKGPIRVT VVEPGYIESE MTAKANSTML MVDNETGVRA MVEAIEKEKG RAVVPRWPWA PLTQVMRLLP PKYTKRFA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcag ccagaggccg caacctggcg ctgtgcgcgc gccgcacgga tcgtctggag gagctcaagg cggagatcgc cgaccggcac ccgtacatca aggtggccat cgccgcgctg gacgtcaacg atcacgaggc cgtgccccgg gtgttcaacg aactgtccga ggaactcggc ggtatcgacc gcgtcatcgt caacgcgggc atcggcaagg gctggccgct gggcgagggc aagccgtggg ccaacaaggc cacgctcgag accaacctgg tcgccggtct ggtgcagatc gaggccgcac tggagatgtt caagaaggca ggcggtggac acctggtgct gatctcctcg gtgctgggca acacgggtgt ccccggcggc aaggccgcct actgtgcgag caaggccggt atgacgtcgc tgggcgagtc cctgcgcgcg gagtacgaca agggccccat cagggtcacg gtcgtcgaac cggggtacat cgaatccgag atgacggcca aggccaactc gacgatgctc atggtcgaca acgagaccgg cgtgcgcgcc atggtcgagg ccatcgagaa ggagaagggg cgcgccgtcg tgccgcgctg gccgtgggcg ccgctgaccc aggtcatgcg gctgttgcca cctaagtaca ccaagcgctt cgcataataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcagccaga ggccgcaacc tggcgctgtg cgcgcgccgc acggatcgtc tggaggagct caaggcggag atcgccgacc ggcacccgta catcaaggtg gccatcgccg cgctggacgt caacgatcac gaggccgtgc cccgggtgtt caacgaactg tccgaggaac tcggcggtat cgaccgcgtc atcgtcaacg cgggcatcgg caagggctgg ccgctgggcg agggcaagcc gtgggccaac aaggccacgc tcgagaccaa cctggtcgcc ggtctggtgc agatcgaggc cgcactggag atgttcaaga aggcaggcgg tggacacctg gtgctgatct cctcggtgct gggcaacacg ggtgtccccg gcggcaaggc cgcctactgt gcgagcaagg ccggtatgac gtcgctgggc gagtccctgc gcgcggagta cgacaagggc cccatcaggg tcacggtcgt cgaaccgggg tacatcgaat ccgagatgac ggccaaggcc aactcgacga tgctcatggt cgacaacgag accggcgtgc gcgccatggt cgaggccatc gagaaggaga aggggcgcgc cgtcgtgccg cgctggccgt gggcgccgct gacccaggtc atgcggctgt tgccacctaa gtacaccaag cgcttcgcaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a