MysmA.01189.d

Oxidoreductase

CENTER ID: MysmA.01189.d
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01189.d.A1.GE28889 Full length( MysmA.01189.d ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3368
RefSeq: YP_887714.1
UniProt: A0QXS6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKAISVEQFG GPDVLELSEV PDPRPAPGEV LISIEASGVG YVDVMAREGH YHAFTEAGFV PGLEIAGVVS ELGDGVDAGW LGRRVFAMPM TGGGYAEYVA VSSDQLIPLP DGVSARSAVA LGMNALVAKI AVERMGSAPG ESVYVRGAGG GIGIMAVQIC NARGAHVTAT TSSALRGERL RALGAEHVVD RHASGESDSR SYDLIVDTVA GPQLGSYVAK LKPNGRYILC GGVGGAPAED FGRVLLENFH RSPTFSAFSL NSVENPAIVT TGTELFAGAD RGAITPVVHC ELPLADAASA HAELEAGRVF GKVVLIP
NT Sequence
GTGAAAGCAA TATCTGTCGA GCAGTTCGGT GGCCCCGATG TTCTCGAACT GTCGGAAGTT CCCGACCCCC GACCCGCTCC CGGTGAAGTG CTCATCAGTA TCGAGGCCTC CGGCGTCGGA TATGTCGATG TCATGGCGCG CGAGGGGCAC TACCACGCCT TCACTGAGGC CGGTTTCGTT CCCGGACTCG AAATCGCCGG CGTGGTGAGC GAACTCGGCG ACGGCGTGGA CGCGGGGTGG CTCGGTCGAC GGGTTTTCGC AATGCCGATG ACGGGTGGCG GATACGCCGA ATATGTTGCG GTGTCCTCCG ATCAGCTCAT CCCGCTTCCC GACGGTGTCT CCGCGCGTTC TGCCGTCGCG CTCGGGATGA ACGCGCTGGT GGCCAAAATC GCGGTCGAAC GGATGGGGTC GGCCCCCGGA GAATCGGTGT ACGTCCGCGG GGCCGGCGGT GGTATCGGCA TCATGGCCGT GCAGATCTGC AACGCGCGCG GAGCGCATGT CACGGCGACC ACGTCATCGG CTCTTCGCGG CGAACGGCTG CGTGCGCTGG GTGCCGAACA CGTCGTCGAT CGCCACGCAT CGGGCGAATC CGATTCGCGG TCCTACGATC TGATCGTGGA CACCGTGGCC GGCCCGCAAC TGGGATCGTA CGTCGCCAAA CTCAAACCGA ACGGCCGATA CATCCTGTGT GGTGGGGTCG GCGGTGCACC TGCGGAAGAT TTCGGTCGGG TGCTGCTCGA AAACTTCCAC CGTTCGCCGA CGTTCTCGGC GTTCAGCCTG AACTCTGTCG AGAACCCGGC CATCGTCACC ACGGGCACCG AACTGTTCGC AGGCGCAGAC CGAGGTGCGA TCACACCGGT TGTCCACTGC GAGTTGCCGC TGGCCGATGC CGCTTCGGCC CACGCCGAAC TCGAGGCGGG GCGGGTGTTC GGCAAAGTGG TACTGATTCC A
Details for MysmA.01189.d.A1.GE28889
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 87
PERCENT COVERAGE: 108
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVKAISVEQ FGGPDVLELS EVPDPRPAPG EVLISIEASG VGYVDVMARE GHYHAFTEAG FVPGLEIAGV VSELGDGVDA GWLGRRVFAM PMTGGGYAEY VAVSSDQLIP LPDGVSARSA VALGMNALVA KIAVERMGSA PGESVYVRGA GGGIGIMAVQ ICNARGAHVT ATTSSALRGE RLRALGAEHV VDRHASGESD SRSYDLIVDT VAGPQLGSYV AXLKPNGRYI LCGGXGGAPA EXFXRVLLEN FXRXPTFSAF XXNSVEXPAX XTTGTXLFAX XRGAXXPXCP LRVAXXDAAX AXXXLXXGGX SXXXXXXXXX XXXXXXXXXX XLXXXXXXXW XXLXXXXX
Validated NT Sequence
annnnnttgt ttactttaag aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg tgaaagcaat atctgtcgag cagttcggtg gccccgatgt tctcgaactg tcggaagttc ccgacccccg acccgctccc ggtgaagtgc tcatcagtat cgaggcctcc ggcgtcggat atgtcgatgt catggcgcgc gaggggcact accacgcctt cactgaggcc ggtttcgttc ccggactcga aatcgccggc gtggtgagcg aactcggcga cggcgtggac gcggggtggc tcggtcgacg ggttttcgca atgccgatga cgggtggcgg atacgccgaa tatgttgcgg tgtcctccga tcagctcatc ccgcttcccg acggtgtctc cgcgcgttct gccgtcgcgc tcgggatgaa cgcgctggtg gccaaaatcg cggtcgaacg gatggggtcg gcccccggag aatcggtgta cgtccgcggg gccggcggtg gtatcggcat catggccgtg cagatctgca acgcgcgcgg agcgcatgtc acggcgacca cgtcatcggc tcttcgcggc gaacggctgc gtgcgctggg tgccgaacac gtcgtcgatc gccacgcatc gggcgaatcc gattcgcggt cctacgatct gatcgtggac accgtggccg gcccgcaact gggatcgtac gtcgccnaac tcaaaccgaa cggccgatac atcctgtgtg gtggggncgg nggtgcacct gcggaagant tcngtcgggt gctgctcgaa aacttccncc gtncgccgac gttctcggcg ttcnnccnga actctgtcga gaanccngcc ntcntcacca cgggcaccnn actgttcgcn ncngancgag gtgcgatnnn nccggnttgt ccactgcgag ttgccnctnn cgatgccgct nnngcccnnc nnnaactcnn nnngggcggg ngntcnncnn nngnnnctnn ttncnnnnnn nnnnannnnn nnnnttnngn nnnnnnnntn cngnnnctan nnnnnnnnnn nnnnnnnnnn tggnnnnnnc tgnnnnnnnn nnnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVKAISVEQ FGGPDVLELS EVPDPRPAPG EVLISIEASG VGYVDVMARE GHYHAFTEAG FVPGLEIAGV VSELGDGVDA GWLGRRVFAM PMTGGGYAEY VAVSSDQLIP LPDGVSARSA VALGMNALVA KIAVERMGSA PGESVYVRGA GGGIGIMAVQ ICNARGAHVT ATTSSALRGE RLRALGAEHV VDRHASGESD SRSYDLIVDT VAGPQLGSYV AKLKPNGRYI LCGGVGGAPA EDFGRVLLEN FHRSPTFSAF SLNSVENPAI VTTGTELFAG ADRGAITPVV HCELPLADAA SAHAELEAGR VFGKVVLIP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaaagca atatctgtcg agcagttcgg tggccccgat gttctcgaac tgtcggaagt tcccgacccc cgacccgctc ccggtgaagt gctcatcagt atcgaggcct ccggcgtcgg atatgtcgat gtcatggcgc gcgaggggca ctaccacgcc ttcactgagg ccggtttcgt tcccggactc gaaatcgccg gcgtggtgag cgaactcggc gacggcgtgg acgcggggtg gctcggtcga cgggttttcg caatgccgat gacgggtggc ggatacgccg aatatgttgc ggtgtcctcc gatcagctca tcccgcttcc cgacggtgtc tccgcgcgtt ctgccgtcgc gctcgggatg aacgcgctgg tggccaaaat cgcggtcgaa cggatggggt cggcccccgg agaatcggtg tacgtccgcg gggccggcgg tggtatcggc atcatggccg tgcagatctg caacgcgcgc ggagcgcatg tcacggcgac cacgtcatcg gctcttcgcg gcgaacggct gcgtgcgctg ggtgccgaac acgtcgtcga tcgccacgca tcgggcgaat ccgattcgcg gtcctacgat ctgatcgtgg acaccgtggc cggcccgcaa ctgggatcgt acgtcgccaa actcaaaccg aacggccgat acatcctgtg tggtggggtc ggcggtgcac ctgcggaaga tttcggtcgg gtgctgctcg aaaacttcca ccgttcgccg acgttctcgg cgttcagcct gaactctgtc gagaacccgg ccatcgtcac cacgggcacc gaactgttcg caggcgcaga ccgaggtgcg atcacaccgg ttgtccactg cgagttgccg ctggccgatg ccgcttcggc ccacgccgaa ctcgaggcgg ggcgggtgtt cggcaaagtg gtactgattc caaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa