SSGCID: Target Page

MysmA.01189.c

Oxidoreductase, zinc-binding dehydrogenase family protein

CENTER ID:	MysmA.01189.c
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01189.c.A1.GE28888	Full length( MysmA.01189.c )		1	323

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01189.c.A1.PW28793	Full length( MysmA.01189.c )		1	323

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2008
RefSeq:	YP_886395.1
UniProt:	A0QU07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRAALITRLD GPDAVEVVEV DEPSGDDAVL IDVHAAGVAF PDALLTRGLY QYKPDLPFSP GAEVAGVVRS APAGASVAAG DRVLGLTMLS GGMAEVVALP EDRVFKLPDA VPFDAGAGIL FNDMTVHFVL RTRGRLSGGE TVLVHGAAGG IGSSVLRLAP ALGAARTIAV VSTEEKGEIA RAAGASDVVL ADGFRDAVKE LTDGRGVDMV VDPVGGDRFT DSLRSLAVGG RLLVVGFTGG EIPQVKVNRL LLNNIEVIGA GWGAWTFTHP GYLQEQWAEL EPLLASGAVA PPQVEVYPLE QAAQAIASLE NRSAKGKVVL KLR

NT Sequence

ATGCGCGCTG CACTGATAAC CCGTCTCGAC GGTCCCGACG CGGTGGAAGT GGTCGAGGTC GACGAACCGT CCGGTGATGA CGCCGTCCTG ATCGACGTCC ACGCCGCAGG CGTGGCGTTT CCCGACGCGC TGCTGACGCG AGGCCTCTAC CAGTACAAGC CGGACCTGCC GTTCTCCCCC GGCGCCGAGG TGGCCGGTGT GGTGCGCAGC GCACCGGCAG GCGCGTCGGT CGCGGCGGGC GATCGTGTGC TGGGCCTGAC GATGCTGTCC GGCGGTATGG CGGAAGTGGT TGCGCTGCCC GAGGATCGCG TGTTCAAGCT GCCCGATGCG GTGCCGTTCG ACGCCGGGGC GGGAATCCTG TTCAACGACA TGACCGTGCA CTTCGTGCTG CGCACCCGCG GCAGGCTATC CGGCGGCGAG ACGGTTCTGG TGCACGGCGC CGCTGGCGGA ATCGGTTCGT CGGTGCTGCG CCTGGCGCCC GCTTTGGGCG CGGCCCGCAC CATCGCGGTG GTGTCCACCG AGGAGAAGGG CGAGATCGCC AGGGCCGCAG GCGCATCCGA TGTGGTGCTG GCCGACGGTT TCCGTGACGC GGTCAAGGAG TTGACCGACG GTCGCGGCGT CGACATGGTG GTCGACCCCG TCGGAGGTGA CCGCTTCACC GATTCGCTGC GCTCGCTGGC GGTCGGCGGG CGCCTGCTGG TGGTCGGCTT CACCGGCGGC GAGATCCCCC AGGTGAAGGT GAACCGGTTG CTGCTCAACA ACATCGAGGT GATCGGCGCG GGTTGGGGTG CGTGGACTTT CACGCACCCG GGTTATCTGC AGGAGCAGTG GGCCGAACTG GAGCCGCTGC TGGCCTCCGG CGCGGTCGCC CCGCCGCAGG TCGAGGTGTA TCCGTTGGAG CAGGCCGCGC AGGCCATCGC GTCACTGGAG AATCGCAGCG CCAAGGGCAA GGTGGTGCTC AAACTGCGC

Details for MysmA.01189.c.A1.GE28888

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMRAALIT RLDGPDAVEV VEVDEPSGDD AVLIDVHAAG VAFPDALLTR GLYQYKPDLP FSPGAEVAGV VRSAPAGASV AAGDRVLGLT MLSGGMAEVV ALPEDRVFKL PDAVPFDAGA GILFNDMTVH FVLRTRGRLS GGETVLVHGA AGGIGSSVLR LAPALGAART IAVVSTEEKG EIARAAGASD VVLADGFRDA VKELTDGRGV DMVVDPVGGD RFTDSLRSLA VGGRLLVVGF TGGEIPQVKV NRLLLNNIEV IGAGWGAWTF THPGYLQEQW AELEPLLASG AVAPPQVEVY PLEQAAQAIA SLENRSAKGK VVLKLR

Validated NT Sequence

ttattagcgc agtttgagca ccaccttgcc cttggcgctg cgattctcca gtgacgcgat ggcctgcgcg gcctgctcca acggatacac ctcgacctgc ggcggggcga ccgcgccgga ggccagcagc ggctccagtt cggcccactg ctcctgcaga taacccgggt gcgtgaaagt ccacgcaccc caacccgcgc cgatcacctc gatgttgttg agcagcaacc ggttcacctt cacctggggg atctcgccgc cggtgaagcc gaccaccagc aggcgcccgc cgaccgccag cgagcgcagc gaatcggtga agcggtcacc tccgacgggg tcgaccacca tgtcgacgcc gcgaccgtcg gtcaactcct tgaccgcgtc acggaaaccg tcggccagca ccacatcgga tgcgcctgcg gccctggcga tctcgccctt ctcctcggtg gacaccaccg cgatggtgcg ggccgcgccc aaagcgggcg ccaggcgcag caccgacgaa ccgattccgc cagcggcgcc gtgcaccaga accgtctcgc cgccggatag cctgccgcgg gtgcgcagca cgaagtgcac ggtcatgtcg ttgaacagga ttcccgcccc ggcgtcgaac ggcaccgcat cgggcagctt gaacacgcga tcctcgggca gcgcaaccac ttccgccata ccgccggaca gcatcgtcag gcccagcaca cgatcgcccg ccgcgaccga cgcgcctgcc ggtgcgctgc gcaccacacc ggccacctcg gcgccggggg agaacggcag gtccggcttg tactggtaga ggcctcgcgt cagcagcgcg tcgggaaacg ccacgcctgc ggcgtggacg tcgatcagga cggcgtcatc accggacggt tcgtcgacct cgaccacttc caccgcgtcg ggaccgtcga gacgggttat cagtgcagcg cgcatcgaac caggaccctg ggtctgagct tccagggtac ccatatgatg g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRAALITRL DGPDAVEVVE VDEPSGDDAV LIDVHAAGVA FPDALLTRGL YQYKPDLPFS PGAEVAGVVR SAPAGASVAA GDRVLGLTML SGGMAEVVAL PEDRVFKLPD AVPFDAGAGI LFNDMTVHFV LRTRGRLSGG ETVLVHGAAG GIGSSVLRLA PALGAARTIA VVSTEEKGEI ARAAGASDVV LADGFRDAVK ELTDGRGVDM VVDPVGGDRF TDSLRSLAVG GRLLVVGFTG GEIPQVKVNR LLLNNIEVIG AGWGAWTFTH PGYLQEQWAE LEPLLASGAV APPQVEVYPL EQAAQAIASL ENRSAKGKVV LKLR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgctgca ctgataaccc gtctcgacgg tcccgacgcg gtggaagtgg tcgaggtcga cgaaccgtcc ggtgatgacg ccgtcctgat cgacgtccac gccgcaggcg tggcgtttcc cgacgcgctg ctgacgcgag gcctctacca gtacaagccg gacctgccgt tctcccccgg cgccgaggtg gccggtgtgg tgcgcagcgc accggcaggc gcgtcggtcg cggcgggcga tcgtgtgctg ggcctgacga tgctgtccgg cggtatggcg gaagtggttg cgctgcccga ggatcgcgtg ttcaagctgc ccgatgcggt gccgttcgac gccggggcgg gaatcctgtt caacgacatg accgtgcact tcgtgctgcg cacccgcggc aggctatccg gcggcgagac ggttctggtg cacggcgccg ctggcggaat cggttcgtcg gtgctgcgcc tggcgcccgc tttgggcgcg gcccgcacca tcgcggtggt gtccaccgag gagaagggcg agatcgccag ggccgcaggc gcatccgatg tggtgctggc cgacggtttc cgtgacgcgg tcaaggagtt gaccgacggt cgcggcgtcg acatggtggt cgaccccgtc ggaggtgacc gcttcaccga ttcgctgcgc tcgctggcgg tcggcgggcg cctgctggtg gtcggcttca ccggcggcga gatcccccag gtgaaggtga accggttgct gctcaacaac atcgaggtga tcggcgcggg ttggggtgcg tggactttca cgcacccggg ttatctgcag gagcagtggg ccgaactgga gccgctgctg gcctccggcg cggtcgcccc gccgcaggtc gaggtgtatc cgttggagca ggccgcgcag gccatcgcgt cactggagaa tcgcagcgcc aagggcaagg tggtgctcaa actgcgcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for MysmA.01189.c.A1.PW28793

PURIFICATION DATe:	6/1/2010
CONCENTRATION:	25.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCGCTGCA CTGATAACCC GTCTCGACGG TCCCGACGCG GTGGAAGTGG TCGAGGTCGA CGAACCGTCC GGTGATGACG CCGTCCTGAT CGACGTCCAC GCCGCAGGCG TGGCGTTTCC CGACGCGCTG CTGACGCGAG GCCTCTACCA GTACAAGCCG GACCTGCCGT TCTCCCCCGG CGCCGAGGTG GCCGGTGTGG TGCGCAGCGC ACCGGCAGGC GCGTCGGTCG CGGCGGGCGA TCGTGTGCTG GGCCTGACGA TGCTGTCCGG CGGTATGGCG GAAGTGGTTG CGCTGCCCGA GGATCGCGTG TTCAAGCTGC CCGATGCGGT GCCGTTCGAC GCCGGGGCGG GAATCCTGTT CAACGACATG ACCGTGCACT TCGTGCTGCG CACCCGCGGC AGGCTATCCG GCGGCGAGAC GGTTCTGGTG CACGGCGCCG CTGGCGGAAT CGGTTCGTCG GTGCTGCGCC TGGCGCCCGC TTTGGGCGCG GCCCGCACCA TCGCGGTGGT GTCCACCGAG GAGAAGGGCG AGATCGCCAG GGCCGCAGGC GCATCCGATG TGGTGCTGGC CGACGGTTTC CGTGACGCGG TCAAGGAGTT GACCGACGGT CGCGGCGTCG ACATGGTGGT CGACCCCGTC GGAGGTGACC GCTTCACCGA TTCGCTGCGC TCGCTGGCGG TCGGCGGGCG CCTGCTGGTG GTCGGCTTCA CCGGCGGCGA GATCCCCCAG GTGAAGGTGA ACCGGTTGCT GCTCAACAAC ATCGAGGTGA TCGGCGCGGG TTGGGGTGCG TGGACTTTCA CGCACCCGGG TTATCTGCAG GAGCAGTGGG CCGAACTGGA GCCGCTGCTG GCCTCCGGCG CGGTCGCCCC GCCGCAGGTC GAGGTGTATC CGTTGGAGCA GGCCGCGCAG GCCATCGCGT CACTGGAGAA TCGCAGCGCC AAGGGCAAGG TGGTGCTCAA ACTGCGCAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA