MysmA.01189.b

Zinc-binding oxidoreductase

CENTER ID: MysmA.01189.b
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01189.b.A1.GE28887 Full length( MysmA.01189.b ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01189.b.A1.PW28792 Full length( MysmA.01189.b ) 1 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5609
RefSeq: YP_889991.1
UniProt: A0R4A3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAIIAKDRT AGIDGLALAE VPYPHAAEND VIVAVHAAAF TPGELDWPGT WTDRAGRDRT PSVPGHEVSG VVAELGFGTT GLAVGQRVFG LTDWCRNGTL AEYVAVEARN LAPLSATIDH TTAAALPISG LTAWQGLFTH GHLTPGETVL IHGAAGGVGS IAVQLAREAG AHVIGSGRTA DREYVLEVGA EAFVDLEHDR LEDVGEVDLV LDVIGGQILY RSAALVRPTG SVVSIVEPPR IPADNGRAVF FVVEPDRAGL VELEQRLRDG RLRPRVGTVY PLADAKEAFA PAQRPRGKSV IRVIEES
NT Sequence
ATGCGAGCGA TCATCGCGAA AGACCGCACA GCAGGCATCG ACGGCCTGGC CCTCGCGGAG GTGCCGTATC CGCATGCCGC CGAGAACGAC GTCATCGTCG CGGTGCATGC GGCGGCCTTC ACACCGGGCG AACTCGACTG GCCGGGCACC TGGACCGACC GCGCCGGACG CGACCGGACA CCGTCGGTTC CCGGTCACGA GGTGTCCGGT GTGGTCGCCG AACTGGGTTT CGGCACCACG GGTCTGGCCG TCGGGCAGCG GGTTTTCGGC CTTACCGACT GGTGCCGCAA CGGCACGCTG GCCGAGTACG TGGCCGTCGA GGCACGCAAC CTCGCACCGT TGTCGGCCAC GATCGACCAC ACCACGGCCG CGGCACTGCC GATCTCCGGG CTCACCGCGT GGCAGGGCCT GTTCACGCAC GGTCACCTCA CACCGGGTGA GACCGTTCTC ATCCACGGTG CGGCAGGCGG AGTGGGATCG ATCGCGGTGC AACTCGCCAG GGAAGCCGGA GCGCACGTCA TCGGCAGCGG CCGTACCGCC GACCGCGAGT ACGTGCTGGA AGTGGGCGCG GAAGCGTTCG TAGACCTGGA ACACGACCGG CTCGAGGATG TCGGCGAGGT CGACCTGGTG CTCGACGTGA TCGGCGGCCA GATCCTCTAC CGCTCAGCGG CTCTCGTGCG CCCTACGGGC TCCGTCGTGA GCATCGTCGA ACCCCCACGC ATACCGGCCG ACAACGGACG AGCAGTGTTC TTCGTCGTCG AACCAGACCG GGCGGGCCTG GTCGAACTCG AACAAAGGCT GCGCGACGGG CGGTTGCGCC CACGCGTCGG AACGGTCTAC CCGCTCGCCG ACGCGAAGGA GGCGTTCGCG CCGGCGCAGC GTCCGCGCGG CAAGTCGGTC ATCCGGGTGA TCGAGGAGTC C
Details for MysmA.01189.b.A1.GE28887
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAIIAKDR TAGIDGLALA EVPYPHAAEN DVIVAVHAAA FTPGELDWPG TWTDRAGRDR TPSVPGHEVS GVVAELGFGT TGLAVGQRVF GLTDWCRNGT LAEYVAVEAR NLAPLSATID HTTAAALPIS GLTAWQGLFT HGHLTPGETV LIHGAAGGVG SIAVQLAREA GAHVIGSGRT ADREYVLEVG AEAFVDLEHD RLEDVGEVDL VLDVIGGQIL YRSAALVRPT GSVVSIVEPP RIPADNGRAV FFVVEPDRAG LVELEQRLRD GRLRPRVGTV YPLADAKEAF APAQRPRGKS VIRVIE
Validated NT Sequence
cctcgatcac ccggatgacc gacttgccgc gcggacgctg cgccggcgcg aacgcctcct tcgcgtcggc gagcgggtag accgttccga cgcgtgggcg caaccgcccg tcgcgcagcc tttgttcgag ttcgaccagg cccgcccggt ctggttcgac gacgaagaac actgctcgtc cgttgtcggc cggtatgcgt gggggttcga cgatgctcac gacggagccc gtagggcgca cgagagccgc tgagcggtag aggatctggc cgccgatcac gtcgagcacc aggtcgacct cgccgacatc ctcgagccgg tcgtgttcca ggtctacgaa cgcttccgcg cccacttcca gcacgtactc gcggtcggcg gtacggccgc tgccgatgac gtgcgctccg gcttccctgg cgagttgcac cgcgatcgat cccactccgc ctgccgcacc gtggatgaga acggtctcac ccggtgtgag gtgaccgtgc gtgaacaggc cctgccacgc ggtgagcccg gagatcggca gtgccgcggc cgtggtgtgg tcgatcgtgg ccgacaacgg tgcgaggttg cgtgcctcga cggccacgta ctcggccagc gtgccgttgc ggcaccagtc ggtaaggccg aaaacccgct gcccgacggc cagacccgtg gtgccgaaac ccagttcggc gaccacaccg gacacctcgt gaccgggaac cgacggtgtc cggtcgcgtc cggcgcggtc ggtccaggtg cccggccagt cgagttcgcc cggtgtgaag gccgccgcat gcaccgcgac gatgacgtcg ttctcggcgg catgcggata cggcacctcc gcgagggcca ggccgtcgat gcctgctgtg cggtctttcg cgatgatcgc tcgcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAIIAKDR TAGIDGLALA EVPYPHAAEN DVIVAVHAAA FTPGELDWPG TWTDRAGRDR TPSVPGHEVS GVVAELGFGT TGLAVGQRVF GLTDWCRNGT LAEYVAVEAR NLAPLSATID HTTAAALPIS GLTAWQGLFT HGHLTPGETV LIHGAAGGVG SIAVQLAREA GAHVIGSGRT ADREYVLEVG AEAFVDLEHD RLEDVGEVDL VLDVIGGQIL YRSAALVRPT GSVVSIVEPP RIPADNGRAV FFVVEPDRAG LVELEQRLRD GRLRPRVGTV YPLADAKEAF APAQRPRGKS VIRVIEES
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgagcgatc atcgcgaaag accgcacagc aggcatcgac ggcctggccc tcgcggaggt gccgtatccg catgccgccg agaacgacgt catcgtcgcg gtgcatgcgg cggccttcac accgggcgaa ctcgactggc cgggcacctg gaccgaccgc gccggacgcg accggacacc gtcggttccc ggtcacgagg tgtccggtgt ggtcgccgaa ctgggtttcg gcaccacggg tctggccgtc gggcagcggg ttttcggcct taccgactgg tgccgcaacg gcacgctggc cgagtacgtg gccgtcgagg cacgcaacct cgcaccgttg tcggccacga tcgaccacac cacggccgcg gcactgccga tctccgggct caccgcgtgg cagggcctgt tcacgcacgg tcacctcaca ccgggtgaga ccgttctcat ccacggtgcg gcaggcggag tgggatcgat cgcggtgcaa ctcgccaggg aagccggagc gcacgtcatc ggcagcggcc gtaccgccga ccgcgagtac gtgctggaag tgggcgcgga agcgttcgta gacctggaac acgaccggct cgaggatgtc ggcgaggtcg acctggtgct cgacgtgatc ggcggccaga tcctctaccg ctcagcggct ctcgtgcgcc ctacgggctc cgtcgtgagc atcgtcgaac ccccacgcat accggccgac aacggacgag cagtgttctt cgtcgtcgaa ccagaccggg cgggcctggt cgaactcgaa caaaggctgc gcgacgggcg gttgcgccca cgcgtcggaa cggtctaccc gctcgccgac gcgaaggagg cgttcgcgcc ggcgcagcgt ccgcgcggca agtcggtcat ccgggtgatc gaggagtcca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.01189.b.A1.PW28792
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 26.81mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRAIIAKDR TAGIDGLALA EVPYPHAAEN DVIVAVHAAA FTPGELDWPG TWTDRAGRDR TPSVPGHEVS GVVAELGFGT TGLAVGQRVF GLTDWCRNGT LAEYVAVEAR NLAPLSATID HTTAAALPIS GLTAWQGLFT HGHLTPGETV LIHGAAGGVG SIAVQLAREA GAHVIGSGRT ADREYVLEVG AEAFVDLEHD RLEDVGEVDL VLDVIGGQIL YRSAALVRPT GSVVSIVEPP RIPADNGRAV FFVVEPDRAG LVELEQRLRD GRLRPRVGTV YPLADAKEAF APAQRPRGKS VIRVIE
Validated NT Sequence
cctcgatcac ccggatgacc gacttgccgc gcggacgctg cgccggcgcg aacgcctcct tcgcgtcggc gagcgggtag accgttccga cgcgtgggcg caaccgcccg tcgcgcagcc tttgttcgag ttcgaccagg cccgcccggt ctggttcgac gacgaagaac actgctcgtc cgttgtcggc cggtatgcgt gggggttcga cgatgctcac gacggagccc gtagggcgca cgagagccgc tgagcggtag aggatctggc cgccgatcac gtcgagcacc aggtcgacct cgccgacatc ctcgagccgg tcgtgttcca ggtctacgaa cgcttccgcg cccacttcca gcacgtactc gcggtcggcg gtacggccgc tgccgatgac gtgcgctccg gcttccctgg cgagttgcac cgcgatcgat cccactccgc ctgccgcacc gtggatgaga acggtctcac ccggtgtgag gtgaccgtgc gtgaacaggc cctgccacgc ggtgagcccg gagatcggca gtgccgcggc cgtggtgtgg tcgatcgtgg ccgacaacgg tgcgaggttg cgtgcctcga cggccacgta ctcggccagc gtgccgttgc ggcaccagtc ggtaaggccg aaaacccgct gcccgacggc cagacccgtg gtgccgaaac ccagttcggc gaccacaccg gacacctcgt gaccgggaac cgacggtgtc cggtcgcgtc cggcgcggtc ggtccaggtg cccggccagt cgagttcgcc cggtgtgaag gccgccgcat gcaccgcgac gatgacgtcg ttctcggcgg catgcggata cggcacctcc gcgagggcca ggccgtcgat gcctgctgtg cggtctttcg cgatgatcgc tcgcatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atgagccatg g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRAIIAKDR TAGIDGLALA EVPYPHAAEN DVIVAVHAAA FTPGELDWPG TWTDRAGRDR TPSVPGHEVS GVVAELGFGT TGLAVGQRVF GLTDWCRNGT LAEYVAVEAR NLAPLSATID HTTAAALPIS GLTAWQGLFT HGHLTPGETV LIHGAAGGVG SIAVQLAREA GAHVIGSGRT ADREYVLEVG AEAFVDLEHD RLEDVGEVDL VLDVIGGQIL YRSAALVRPT GSVVSIVEPP RIPADNGRAV FFVVEPDRAG LVELEQRLRD GRLRPRVGTV YPLADAKEAF APAQRPRGKS VIRVIEES
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGAGCGATC ATCGCGAAAG ACCGCACAGC AGGCATCGAC GGCCTGGCCC TCGCGGAGGT GCCGTATCCG CATGCCGCCG AGAACGACGT CATCGTCGCG GTGCATGCGG CGGCCTTCAC ACCGGGCGAA CTCGACTGGC CGGGCACCTG GACCGACCGC GCCGGACGCG ACCGGACACC GTCGGTTCCC GGTCACGAGG TGTCCGGTGT GGTCGCCGAA CTGGGTTTCG GCACCACGGG TCTGGCCGTC GGGCAGCGGG TTTTCGGCCT TACCGACTGG TGCCGCAACG GCACGCTGGC CGAGTACGTG GCCGTCGAGG CACGCAACCT CGCACCGTTG TCGGCCACGA TCGACCACAC CACGGCCGCG GCACTGCCGA TCTCCGGGCT CACCGCGTGG CAGGGCCTGT TCACGCACGG TCACCTCACA CCGGGTGAGA CCGTTCTCAT CCACGGTGCG GCAGGCGGAG TGGGATCGAT CGCGGTGCAA CTCGCCAGGG AAGCCGGAGC GCACGTCATC GGCAGCGGCC GTACCGCCGA CCGCGAGTAC GTGCTGGAAG TGGGCGCGGA AGCGTTCGTA GACCTGGAAC ACGACCGGCT CGAGGATGTC GGCGAGGTCG ACCTGGTGCT CGACGTGATC GGCGGCCAGA TCCTCTACCG CTCAGCGGCT CTCGTGCGCC CTACGGGCTC CGTCGTGAGC ATCGTCGAAC CCCCACGCAT ACCGGCCGAC AACGGACGAG CAGTGTTCTT CGTCGTCGAA CCAGACCGGG CGGGCCTGGT CGAACTCGAA CAAAGGCTGC GCGACGGGCG GTTGCGCCCA CGCGTCGGAA CGGTCTACCC GCTCGCCGAC GCGAAGGAGG CGTTCGCGCC GGCGCAGCGT CCGCGCGGCA AGTCGGTCAT CCGGGTGATC GAGGAGTCCA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A