MysmA.01189.a

Alcohol dehydrogenase, zinc-binding

CENTER ID: MysmA.01189.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01189.a.A1.GE28886 Full length( MysmA.01189.a ) 1 324
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01189.a.A1.PW28791 Full length( MysmA.01189.a ) 1 324

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4712
RefSeq: YP_889084.1
UniProt: A0R1P6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRAAVCPRYG PPEVVRVEEL PVPELGPDQV RVRVGAAAVN FPDVLLVADR YQATVPVPFV PGSEFAGVVT ETADGVTDFA VGDRVVGTGM HGAFAEEVCV DASCLQRAVE RLDDRGAAAS GVAHRTAFHA LRSVARVSTG DDVVVLGAGG GVGLAAVQIA AALGARVTAV ASSSEKLDAA ARYGAHHLVD HTAGPLRAAL RDAVPDGADA VLDPVGGDLS EPALRALRRG GRFVTIGFAS GAIPRIPLNL VLVKGVQILG FAFQDVPPEE FARNESELGE LLASGAVTPH IGAVYPLVEV AAALRHVAEG RAVGKVVLDV SRVR
NT Sequence
GTGCGCGCAG CCGTCTGCCC GCGGTACGGG CCACCCGAGG TCGTCCGCGT CGAGGAACTG CCTGTGCCTG AACTCGGGCC CGATCAGGTG CGGGTGCGTG TCGGTGCTGC CGCGGTGAAC TTTCCCGACG TGTTGCTCGT GGCCGACAGG TACCAGGCGA CCGTGCCCGT GCCGTTCGTG CCTGGCAGTG AGTTCGCCGG GGTGGTGACC GAGACCGCCG ACGGTGTCAC GGATTTCGCA GTCGGCGACC GGGTGGTCGG CACCGGGATG CACGGCGCGT TCGCCGAGGA GGTGTGTGTC GACGCGTCCT GTCTGCAGCG CGCCGTCGAG CGCCTCGACG ACCGTGGCGC GGCCGCGTCC GGCGTCGCGC ACCGCACCGC GTTCCACGCG CTGCGGTCGG TCGCCCGGGT CTCCACGGGC GACGACGTTG TCGTGCTTGG CGCAGGCGGC GGCGTCGGCC TGGCCGCGGT GCAGATCGCC GCGGCACTCG GCGCCCGGGT GACCGCGGTG GCGTCCTCAT CCGAAAAACT CGATGCCGCA GCACGATACG GTGCACACCA CCTCGTCGAC CACACAGCCG GGCCGCTCCG CGCGGCACTG CGCGACGCTG TTCCCGACGG TGCCGACGCG GTGCTCGACC CGGTCGGCGG CGACCTGTCC GAACCCGCGT TGCGCGCGTT GCGCCGCGGC GGGCGGTTCG TCACCATCGG CTTCGCGTCA GGCGCCATCC CCCGGATACC GCTGAACCTC GTGCTGGTGA AGGGCGTGCA GATCCTGGGC TTCGCGTTCC AGGACGTGCC GCCGGAGGAA TTCGCGCGCA ACGAATCCGA GCTCGGCGAA CTGCTGGCGT CCGGCGCCGT GACGCCGCAC ATCGGTGCGG TCTATCCCCT CGTCGAGGTC GCTGCCGCGC TGCGGCATGT CGCCGAGGGG CGCGCCGTGG GCAAGGTGGT CCTGGACGTC TCTCGCGTTC GA
Details for MysmA.01189.a.A1.GE28886
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMVRAAVC PRYGPPEVVR VEELPVPELG PDQVRVRVGA AAVNFPDVLL VADRYQATVP VPFVPGSEFA GVVTETADGV TDFAVGDRVV GTGMHGAFAE EVCVDASCLQ RAVERLDDRG AAASGVAHRT AFHALRSVAR VSTGDDVVVL GAGGGVGLAA VQIAAALGAR VTAVASSSEK LDAAARYGAH HLVDHTAGPL RAALRDAVPD GADAVLDPVG GDLSEPALRA LRRGGRFVTI GFASGAIPRI PLNLVLVKGV QILGFAFQDV PPEEFARNES ELGELLASGA VTPHIGAVYP LVEVAAALRH VAEGRAVGKV VLDVSRVR
Validated NT Sequence
ttattatcga acgcgagaga cgtccaggac caccttgccc acggcgcgcc cctcggcgac atgccgcagc gcggcagcga cctcgacgag gggatagacc gcaccgatgt gcggcgtcac ggcgccggac gccagcagtt cgccgagctc ggattcgttg cgcgcgaatt cctccggcgg cacgtcctgg aacgcgaagc ccaggatctg cacgcccttc accagcacga ggttcagcgg tatccggggg atggcgcctg acgcgaagcc gatggtgacg aaccgcccgc cgcggcgcaa cgcgcgcaac gcgggttcgg acaggtcgcc gccgaccggg tcgagcaccg cgtcggcacc gtcgggaaca gcgtcgcgca gtgccgcgcg gagcggcccg gctgtgtggt cgacgaggtg gtgtgcaccg tatcgtgctg cggcatcgag tttttcggat gaggacgcca ccgcggtcac ccgggcgccg agtgccgcgg cgatctgcac cgcggccagg ccgacgccgc cgcctgcgcc aagcacgaca acgtcgtcgc ccgtggagac ccgggcgacc gaccgcagcg cgtggaacgc ggtgcggtgc gcgacgccgg acgcggccgc gccacggtcg tcgaggcgct cgacggcgcg ctgcagacag gacgcgtcga cacacacctc ctcggcgaac gcgccgtgca tcccggtgcc gaccacccgg tcgccgactg cgaaatccgt gacaccgtcg gcggtctcgg tcaccacccc ggcgaactca ctgccaggca cgaacggcac gggcacggtc gcctggtacc tgtcggccac gagcaacacg tcgggaaagt tcaccgcggc agcaccgaca cgcacccgca cctgatcggg cccgagttca ggcacaggca gttcctcgac gcggacgacc tcgggtggcc cgtaccgcgg gcagacggct gcgcgcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRAAVCPR YGPPEVVRVE ELPVPELGPD QVRVRVGAAA VNFPDVLLVA DRYQATVPVP FVPGSEFAGV VTETADGVTD FAVGDRVVGT GMHGAFAEEV CVDASCLQRA VERLDDRGAA ASGVAHRTAF HALRSVARVS TGDDVVVLGA GGGVGLAAVQ IAAALGARVT AVASSSEKLD AAARYGAHHL VDHTAGPLRA ALRDAVPDGA DAVLDPVGGD LSEPALRALR RGGRFVTIGF ASGAIPRIPL NLVLVKGVQI LGFAFQDVPP EEFARNESEL GELLASGAVT PHIGAVYPLV EVAAALRHVA EGRAVGKVVL DVSRVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgcgca gccgtctgcc cgcggtacgg gccacccgag gtcgtccgcg tcgaggaact gcctgtgcct gaactcgggc ccgatcaggt gcgggtgcgt gtcggtgctg ccgcggtgaa ctttcccgac gtgttgctcg tggccgacag gtaccaggcg accgtgcccg tgccgttcgt gcctggcagt gagttcgccg gggtggtgac cgagaccgcc gacggtgtca cggatttcgc agtcggcgac cgggtggtcg gcaccgggat gcacggcgcg ttcgccgagg aggtgtgtgt cgacgcgtcc tgtctgcagc gcgccgtcga gcgcctcgac gaccgtggcg cggccgcgtc cggcgtcgcg caccgcaccg cgttccacgc gctgcggtcg gtcgcccggg tctccacggg cgacgacgtt gtcgtgcttg gcgcaggcgg cggcgtcggc ctggccgcgg tgcagatcgc cgcggcactc ggcgcccggg tgaccgcggt ggcgtcctca tccgaaaaac tcgatgccgc agcacgatac ggtgcacacc acctcgtcga ccacacagcc gggccgctcc gcgcggcact gcgcgacgct gttcccgacg gtgccgacgc ggtgctcgac ccggtcggcg gcgacctgtc cgaacccgcg ttgcgcgcgt tgcgccgcgg cgggcggttc gtcaccatcg gcttcgcgtc aggcgccatc ccccggatac cgctgaacct cgtgctggtg aagggcgtgc agatcctggg cttcgcgttc caggacgtgc cgccggagga attcgcgcgc aacgaatccg agctcggcga actgctggcg tccggcgccg tgacgccgca catcggtgcg gtctatcccc tcgtcgaggt cgctgccgcg ctgcggcatg tcgccgaggg gcgcgccgtg ggcaaggtgg tcctggacgt ctctcgcgtt cgaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MysmA.01189.a.A1.PW28791
PURIFICATION DATe: 6/1/2010
CONCENTRATION: 28.01mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MGTLEAQTQG PGSMVRAAVC PRYGPPEVVR VEELPVPELG PDQVRVRVGA AAVNFPDVLL VADRYQATVP VPFVPGSEFA GVVTETADGV TDFAVGDRVV GTGMHGAFAE EVCVDASCLQ RAVERLDDRG AAASGVAHRT AFHALRSVAR VSTGDDVVVL GAGGGVGLAA VQIAAALGAR VTAVASSSEK LDAAARYGAH HLVDHTAGPL RAALRDAVPD GADAVLDPVG GDLSEPALRA LRRGGRFVTI GFASGAIPRI PLNLVLVKGV QILGFAFQDV PPEEFARNES ELGELLASGA VTPHIGAVYP LVEVAAALRH VAEGRAVGKV VLDVSRVR
Validated NT Sequence
ttattatcga acgcgagaga cgtccaggac caccttgccc acggcgcgcc cctcggcgac atgccgcagc gcggcagcga cctcgacgag gggatagacc gcaccgatgt gcggcgtcac ggcgccggac gccagcagtt cgccgagctc ggattcgttg cgcgcgaatt cctccggcgg cacgtcctgg aacgcgaagc ccaggatctg cacgcccttc accagcacga ggttcagcgg tatccggggg atggcgcctg acgcgaagcc gatggtgacg aaccgcccgc cgcggcgcaa cgcgcgcaac gcgggttcgg acaggtcgcc gccgaccggg tcgagcaccg cgtcggcacc gtcgggaaca gcgtcgcgca gtgccgcgcg gagcggcccg gctgtgtggt cgacgaggtg gtgtgcaccg tatcgtgctg cggcatcgag tttttcggat gaggacgcca ccgcggtcac ccgggcgccg agtgccgcgg cgatctgcac cgcggccagg ccgacgccgc cgcctgcgcc aagcacgaca acgtcgtcgc ccgtggagac ccgggcgacc gaccgcagcg cgtggaacgc ggtgcggtgc gcgacgccgg acgcggccgc gccacggtcg tcgaggcgct cgacggcgcg ctgcagacag gacgcgtcga cacacacctc ctcggcgaac gcgccgtgca tcccggtgcc gaccacccgg tcgccgactg cgaaatccgt gacaccgtcg gcggtctcgg tcaccacccc ggcgaactca ctgccaggca cgaacggcac gggcacggtc gcctggtacc tgtcggccac gagcaacacg tcgggaaagt tcaccgcggc agcaccgaca cgcacccgca cctgatcggg cccgagttca ggcacaggca gttcctcgac gcggacgacc tcgggtggcc cgtaccgcgg gcagacggct gcgcgcacca tcgaaccagg accctgggtc tgagcttcca gggtacccat at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRAAVCPR YGPPEVVRVE ELPVPELGPD QVRVRVGAAA VNFPDVLLVA DRYQATVPVP FVPGSEFAGV VTETADGVTD FAVGDRVVGT GMHGAFAEEV CVDASCLQRA VERLDDRGAA ASGVAHRTAF HALRSVARVS TGDDVVVLGA GGGVGLAAVQ IAAALGARVT AVASSSEKLD AAARYGAHHL VDHTAGPLRA ALRDAVPDGA DAVLDPVGGD LSEPALRALR RGGRFVTIGF ASGAIPRIPL NLVLVKGVQI LGFAFQDVPP EEFARNESEL GELLASGAVT PHIGAVYPLV EVAAALRHVA EGRAVGKVVL DVSRVR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGCGCA GCCGTCTGCC CGCGGTACGG GCCACCCGAG GTCGTCCGCG TCGAGGAACT GCCTGTGCCT GAACTCGGGC CCGATCAGGT GCGGGTGCGT GTCGGTGCTG CCGCGGTGAA CTTTCCCGAC GTGTTGCTCG TGGCCGACAG GTACCAGGCG ACCGTGCCCG TGCCGTTCGT GCCTGGCAGT GAGTTCGCCG GGGTGGTGAC CGAGACCGCC GACGGTGTCA CGGATTTCGC AGTCGGCGAC CGGGTGGTCG GCACCGGGAT GCACGGCGCG TTCGCCGAGG AGGTGTGTGT CGACGCGTCC TGTCTGCAGC GCGCCGTCGA GCGCCTCGAC GACCGTGGCG CGGCCGCGTC CGGCGTCGCG CACCGCACCG CGTTCCACGC GCTGCGGTCG GTCGCCCGGG TCTCCACGGG CGACGACGTT GTCGTGCTTG GCGCAGGCGG CGGCGTCGGC CTGGCCGCGG TGCAGATCGC CGCGGCACTC GGCGCCCGGG TGACCGCGGT GGCGTCCTCA TCCGAAAAAC TCGATGCCGC AGCACGATAC GGTGCACACC ACCTCGTCGA CCACACAGCC GGGCCGCTCC GCGCGGCACT GCGCGACGCT GTTCCCGACG GTGCCGACGC GGTGCTCGAC CCGGTCGGCG GCGACCTGTC CGAACCCGCG TTGCGCGCGT TGCGCCGCGG CGGGCGGTTC GTCACCATCG GCTTCGCGTC AGGCGCCATC CCCCGGATAC CGCTGAACCT CGTGCTGGTG AAGGGCGTGC AGATCCTGGG CTTCGCGTTC CAGGACGTGC CGCCGGAGGA ATTCGCGCGC AACGAATCCG AGCTCGGCGA ACTGCTGGCG TCCGGCGCCG TGACGCCGCA CATCGGTGCG GTCTATCCCC TCGTCGAGGT CGCTGCCGCG CTGCGGCATG TCGCCGAGGG GCGCGCCGTG GGCAAGGTGG TCCTGGACGT CTCTCGCGTT CGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA