SSGCID: Target Page

MysmA.01155.a

HAD-superfamily protein subfamily protein IB hydrolase

CENTER ID:	MysmA.01155.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01155.a.A1.GE28881	Full length( MysmA.01155.a )		1	223

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3470
RefSeq:	YP_887820.1
UniProt:	A0QY32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAFFDLDGT LVDGFTATAH AGHRIRRRQA AFGEVLGIIE ASVRYRIGRM PFERLLVRAA GYLRGEALAD LDVLGEELFE NRISPRVYPH MRDVVRKHQD RGHTVVLSSS ALTIHAEPVA RHLGIDHVLC NHFETDEHGR LTGDIVKPVI WGARKAEVVQ NFCVQNSVEL RHSFFYADGA EDCALMHLVG HPRPVNPRPG LATAAAEHGW PVLSLARMPA RRW

NT Sequence

GTGGGGGCGT TCTTCGACCT GGACGGCACC CTCGTCGACG GCTTCACCGC GACGGCCCAC GCCGGTCACC GCATCCGCCG CAGGCAGGCC GCCTTCGGCG AAGTCCTCGG GATCATCGAG GCGTCGGTGC GCTACCGCAT CGGCCGTATG CCCTTCGAAC GGCTGCTCGT TCGTGCCGCG GGATATCTGC GGGGCGAGGC GCTGGCCGAC CTCGACGTGC TGGGCGAGGA ACTGTTCGAA AACCGCATAT CGCCTCGCGT CTATCCGCAC ATGCGCGACG TCGTGCGCAA GCATCAGGAC CGCGGCCACA CCGTGGTGCT GAGTTCCTCG GCGCTGACCA TCCACGCCGA ACCGGTCGCG CGGCATCTGG GTATCGACCA CGTGCTGTGC AACCACTTCG AAACCGACGA GCACGGGCGG CTGACCGGGG ACATCGTCAA ACCGGTGATC TGGGGGGCGC GCAAAGCCGA AGTGGTGCAG AACTTCTGCG TCCAGAACTC GGTCGAGCTG CGACACAGTT TCTTCTACGC CGACGGCGCC GAGGACTGTG CGCTCATGCA TCTGGTGGGC CACCCCCGCC CGGTCAACCC TCGCCCCGGC CTGGCGACGG CCGCGGCCGA ACACGGCTGG CCGGTGCTGT CGCTCGCGCG GATGCCCGCG CGGCGCTGG

Details for MysmA.01155.a.A1.GE28881

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVGAFFDLD GTLVDGFTAT AHAGHRIRRR QAAFGEVLGI IEASVRYRIG RMPFERLLVR AAGYLRGEAL ADLDVLGEEL FENRISPRVY PHMRDVVRKH QDRGHTVVLS SSALTIHAEP VARHLGIDHV LCNHFETDEH GRLTGDIVKP VIWGARKAEV VQNFCVQNSV ELRHSFFYAD GAEDCALMHL VGHPRPVNPR PGLATAAAEH GWPVLSLARM PARRW

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg gggcgttctt cgacctggac ggcaccctcg tcgacggctt caccgcgacg gcccacgccg gtcaccgcat ccgccgcagg caggccgcct tcggcgaagt cctcgggatc atcgaggcgt cggtgcgcta ccgcatcggc cgtatgccct tcgaacggct gctcgttcgt gccgcgggat atctgcgggg cgaggcgctg gccgacctcg acgtgctggg cgaggaactg ttcgaaaacc gcatatcgcc tcgcgtctat ccgcacatgc gcgacgtcgt gcgcaagcat caggaccgcg gccacaccgt ggtgctgagt tcctcggcgc tgaccatcca cgccgaaccg gtcgcgcggc atctgggtat cgaccacgtg ctgtgcaacc acttcgaaac cgacgagcac gggcggctga ccggggacat cgtcaaaccg gtgatctggg gggcgcgcaa agccgaagtg gtgcagaact tctgcgtcca gaactcggtc gagctgcgac acagtttctt ctacgccgac ggcgccgagg actgtgcgct catgcatctg gtgggccacc cccgcccggt caaccctcgc cccggcctgg cgacggccgc ggccgaacac ggctggccgg tgctgtcgct cgcgcggatg cccgcgcggc gctggtaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggggcg ttcttcgacc tggacggcac cctcgtcgac ggcttcaccg cgacggccca cgccggtcac cgcatccgcc gcaggcaggc cgccttcggc gaagtcctcg ggatcatcga ggcgtcggtg cgctaccgca tcggccgtat gcccttcgaa cggctgctcg ttcgtgccgc gggatatctg cggggcgagg cgctggccga cctcgacgtg ctgggcgagg aactgttcga aaaccgcata tcgcctcgcg tctatccgca catgcgcgac gtcgtgcgca agcatcagga ccgcggccac accgtggtgc tgagttcctc ggcgctgacc atccacgccg aaccggtcgc gcggcatctg ggtatcgacc acgtgctgtg caaccacttc gaaaccgacg agcacgggcg gctgaccggg gacatcgtca aaccggtgat ctggggggcg cgcaaagccg aagtggtgca gaacttctgc gtccagaact cggtcgagct gcgacacagt ttcttctacg ccgacggcgc cgaggactgt gcgctcatgc atctggtggg ccacccccgc ccggtcaacc ctcgccccgg cctggcgacg gccgcggccg aacacggctg gccggtgctg tcgctcgcgc ggatgcccgc gcggcgctgg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa