MysmA.01147.a

Cystathionine beta-synthase

CENTER ID: MysmA.01147.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01147.a.A1.PW28784 Full length( MysmA.01147.a ) 1 463

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5141
RefSeq: YP_889516.1
UniProt: A0R2X8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIARHISEL IGNTPLVQLN SVVPEGAGLV AAKIEYLNPG GSSKDRIALK MIEAAEVSGE LKPGGTIVEP TSGNTGVGLA LVAQQRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPDHPDSYYS VSNRLVTEIE GAWKPDQYSN QMGPESHYET TGPEIWADTE GKITHFVAGV GTGGTITGTG RYLKEVSGGK VKVIGADPEG SVYSGGTGRP YLVEGVGEDF WPTAYDPSVP DEIIAVSDAD SFEMTRRLAR EEALLVGGSC GMAVVAAIEV ARKAGPDAVV VVLLPDGGRG YLSKIFNDAW MSSYGFLRTR LDGSVEKTVG DVLRGKSGAL PDLVHTHPSE TVRDAIGILR EYGVSQMPVV GAEPPVMAGE VAGSVSEREL LSAVFEGRAK LADAVAEHMS PPLPLIGAGE LVSTAAKTLR ESDAVMVVED GKPVGVLTRH DLLGFLSEGG YRH
NT Sequence
ATGCGCATAG CCAGGCACAT CAGTGAGCTC ATCGGTAACA CCCCGCTGGT CCAGCTGAAC TCGGTGGTCC CCGAAGGGGC AGGCCTGGTG GCGGCGAAAA TCGAGTACCT CAACCCCGGC GGCAGCTCCA AGGACCGCAT CGCGCTCAAG ATGATCGAGG CCGCCGAGGT CAGCGGTGAG CTCAAACCGG GCGGCACCAT CGTCGAACCC ACCTCGGGCA ACACCGGCGT CGGCCTGGCC CTGGTGGCCC AGCAGCGCGG CTACAAGTGC GTCTTCGTCT GCCCTGACAA GGTCAGCGAG GACAAGCGGA ATGTCCTGCG CGCGTACGGC GCCGAGGTCG TGGTGTGCCC GACGGCGGTT CCGCCGGACC ATCCGGACAG CTACTACAGC GTCTCCAACC GCCTGGTCAC CGAGATCGAG GGCGCCTGGA AGCCCGACCA GTACTCCAAC CAGATGGGCC CGGAGAGCCA CTACGAGACC ACCGGCCCCG AGATCTGGGC CGACACCGAA GGCAAGATCA CACACTTCGT CGCGGGTGTG GGCACCGGCG GGACCATCAC CGGCACCGGC CGCTACCTCA AGGAGGTCTC GGGCGGCAAG GTCAAGGTCA TCGGCGCCGA TCCCGAGGGC TCGGTGTACT CGGGCGGCAC GGGCCGGCCC TACCTCGTCG AAGGTGTCGG CGAGGACTTC TGGCCCACGG CCTACGATCC TTCGGTCCCC GACGAGATCA TCGCGGTGTC CGACGCCGAC TCGTTCGAGA TGACCCGGCG ACTCGCACGT GAAGAGGCCC TGCTGGTCGG CGGATCGTGC GGCATGGCCG TCGTCGCCGC CATCGAGGTG GCCCGCAAGG CCGGGCCCGA CGCCGTGGTC GTGGTGCTGC TGCCCGACGG TGGACGTGGT TATCTGTCAA AGATTTTCAA CGACGCGTGG ATGTCGTCCT ACGGTTTCCT GCGCACCCGC CTCGACGGCA GCGTCGAGAA GACCGTCGGT GACGTGTTGC GCGGCAAGTC GGGTGCACTG CCCGACCTGG TGCACACGCA TCCGTCGGAA ACCGTGCGCG ACGCGATCGG GATCCTGCGC GAGTACGGCG TCTCCCAGAT GCCCGTCGTC GGCGCGGAGC CGCCCGTGAT GGCAGGCGAG GTGGCAGGCA GCGTCTCCGA GCGCGAACTG CTCTCGGCGG TGTTCGAGGG CCGCGCCAAG CTGGCCGACG CGGTCGCCGA GCACATGAGC CCGCCGCTGC CGCTGATCGG CGCCGGTGAA CTGGTCAGCA CGGCCGCCAA GACCCTTCGT GAATCCGACG CCGTGATGGT GGTCGAGGAC GGCAAGCCCG TCGGTGTGCT GACCAGGCAC GACCTGCTGG GGTTCCTGTC CGAGGGCGGT TACCGGCAC
Details for MysmA.01147.a.A1.PW28784
PURIFICATION DATe: 6/7/2010
CONCENTRATION: 26.33mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 10
VIAL VOLUME: 100µl
PERCENT IDENTITY: 83
PERCENT COVERAGE: 80
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRIARHISE LIGNTPLVQL NSVVPEGAGL VAAKIEYLNP GGSSKDRIAL KMIEAAEVSG ELKPGGTIVE PTSGNTGVGL ALVAQQRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPDHPDSYY SVSNRLVTEI EGAWKPDQYS NQMGPESHYE TTGPEIWADT EGKITHFVAG VGTGGTITGT GRYLKEVSGG KVKVIGADPE GSVYSGGTGR PYLVEXVGED FWPTAYDPSV PDEIIAVSDA DSFEMTRRLA REEALLVGGS CGMAVVAAIE VARXXGXDXV XVXLLPXXGR GXLSKIFXDX XXSSXXFLXT XXXXAXXXXX XXXXXXXCXX XXXXXHXXXX XXXXXXXXXX XXXXXXXXX
Validated NT Sequence
tannnnnnnt tnactttang naggaganat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgc gcatagccag gcacatcagt gagctcatcg gtaacacccc gctggtccag ctgaactcgg tggtccccga aggggcaggc ctggtggcgg cgaaaatcga gtacctcaac cccggcggca gctccaagga ccgcatcgcg ctcaagatga tcgaggccgc cgaggtcagc ggtgagctca aaccgggcgg caccatcgtc gaacccacct cgggcaacac cggcgtcggc ctggccctgg tggcccagca gcgcggctac aagtgcgtct tcgtctgccc tgacaaggtc agcgaggaca agcggaatgt cctgcgcgcg tacggcgccg aggtcgtggt gtgcccgacg gcggttccgc cggaccatcc ggacagctac tacagcgtct ccaaccgcct ggtcaccgag atcgagggcg cctggaagcc cgaccagtac tccaaccaga tgggcccgga gagccactac gagaccaccg gccccgagat ctgggccgac accgaaggca agatcacaca cttcgtcgcg ggtgtgggca ccggcgggac catcaccggc accggccgct acctcaagga ggtctcgggc ggcaaggtca aggtcatcgg cgccgatccc gagggctcgg tgtactcggg cggcacgggc cggccctacc tcgtcgaang tgtcggcgag gacttctggc ccacggccta cgatccttcg gtccccgacg agatcatcgc ggtgtccgac gccgactcgt tcgagatgac ccggcgactc gcacgtgaag aggccctgct ggtcggcgga tcgtgcggca tggccgtcgt cgccgccatc gaggtggccc gcangnncgg gnccgacncc gtggncgtgg ngctgctgcc cgangnnggn cgtggnnntc tgtcaaagat tttcancgac ncgnnnnngt cgtcctangn nttcctgnnn acccncntnn annnagcnnc nanannnnnn gntgannnnn nnnnnnnngn nngntgcnnt nnnnnacnnn nnnnnnnnca tcnnnnnnnn nnnnnnnncn nnngnnnnnn nnnnnnnnnn gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIARHISE LIGNTPLVQL NSVVPEGAGL VAAKIEYLNP GGSSKDRIAL KMIEAAEVSG ELKPGGTIVE PTSGNTGVGL ALVAQQRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPDHPDSYY SVSNRLVTEI EGAWKPDQYS NQMGPESHYE TTGPEIWADT EGKITHFVAG VGTGGTITGT GRYLKEVSGG KVKVIGADPE GSVYSGGTGR PYLVEGVGED FWPTAYDPSV PDEIIAVSDA DSFEMTRRLA REEALLVGGS CGMAVVAAIE VARKAGPDAV VVVLLPDGGR GYLSKIFNDA WMSSYGFLRT RLDGSVEKTV GDVLRGKSGA LPDLVHTHPS ETVRDAIGIL REYGVSQMPV VGAEPPVMAG EVAGSVSERE LLSAVFEGRA KLADAVAEHM SPPLPLIGAG ELVSTAAKTL RESDAVMVVE DGKPVGVLTR HDLLGFLSEG GYRH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCATAGCC AGGCACATCA GTGAGCTCAT CGGTAACACC CCGCTGGTCC AGCTGAACTC GGTGGTCCCC GAAGGGGCAG GCCTGGTGGC GGCGAAAATC GAGTACCTCA ACCCCGGCGG CAGCTCCAAG GACCGCATCG CGCTCAAGAT GATCGAGGCC GCCGAGGTCA GCGGTGAGCT CAAACCGGGC GGCACCATCG TCGAACCCAC CTCGGGCAAC ACCGGCGTCG GCCTGGCCCT GGTGGCCCAG CAGCGCGGCT ACAAGTGCGT CTTCGTCTGC CCTGACAAGG TCAGCGAGGA CAAGCGGAAT GTCCTGCGCG CGTACGGCGC CGAGGTCGTG GTGTGCCCGA CGGCGGTTCC GCCGGACCAT CCGGACAGCT ACTACAGCGT CTCCAACCGC CTGGTCACCG AGATCGAGGG CGCCTGGAAG CCCGACCAGT ACTCCAACCA GATGGGCCCG GAGAGCCACT ACGAGACCAC CGGCCCCGAG ATCTGGGCCG ACACCGAAGG CAAGATCACA CACTTCGTCG CGGGTGTGGG CACCGGCGGG ACCATCACCG GCACCGGCCG CTACCTCAAG GAGGTCTCGG GCGGCAAGGT CAAGGTCATC GGCGCCGATC CCGAGGGCTC GGTGTACTCG GGCGGCACGG GCCGGCCCTA CCTCGTCGAA GGTGTCGGCG AGGACTTCTG GCCCACGGCC TACGATCCTT CGGTCCCCGA CGAGATCATC GCGGTGTCCG ACGCCGACTC GTTCGAGATG ACCCGGCGAC TCGCACGTGA AGAGGCCCTG CTGGTCGGCG GATCGTGCGG CATGGCCGTC GTCGCCGCCA TCGAGGTGGC CCGCAAGGCC GGGCCCGACG CCGTGGTCGT GGTGCTGCTG CCCGACGGTG GACGTGGTTA TCTGTCAAAG ATTTTCAACG ACGCGTGGAT GTCGTCCTAC GGTTTCCTGC GCACCCGCCT CGACGGCAGC GTCGAGAAGA CCGTCGGTGA CGTGTTGCGC GGCAAGTCGG GTGCACTGCC CGACCTGGTG CACACGCATC CGTCGGAAAC CGTGCGCGAC GCGATCGGGA TCCTGCGCGA GTACGGCGTC TCCCAGATGC CCGTCGTCGG CGCGGAGCCG CCCGTGATGG CAGGCGAGGT GGCAGGCAGC GTCTCCGAGC GCGAACTGCT CTCGGCGGTG TTCGAGGGCC GCGCCAAGCT GGCCGACGCG GTCGCCGAGC ACATGAGCCC GCCGCTGCCG CTGATCGGCG CCGGTGAACT GGTCAGCACG GCCGCCAAGA CCCTTCGTGA ATCCGACGCC GTGATGGTGG TCGAGGACGG CAAGCCCGTC GGTGTGCTGA CCAGGCACGA CCTGCTGGGG TTCCTGTCCG AGGGCGGTTA CCGGCACAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA