MysmA.01124.a

ABC-type drug export system, ATP-binding protein

CENTER ID: MysmA.01124.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01124.a.A1.GE28877 Full length( MysmA.01124.a ) 1 289
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6333
RefSeq: YP_890722.1
UniProt: A0R6D4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDHMPEPTL PDRTPAIDCD GLTHRYGQFT AVEDLTLQVR AGETLGLLGP NGAGKTTAVR VLTTLTPVQQ GRVRIFGLDT RAHTMDIRHN IGYVPQQLSI EAALTGRQNV ELFARLYDVP RSERADRVAA ALEAMQLLDV ADTVAGTYSG GMVRRLELAQ ALVNRPLLLI LDEPTVGLDP IARDSVWTQV RAMQAEFGMT VLLTTHYMGE ADALCDRVAL MHHGRLQAVG SPDELKRAVS SQASDDAGET TLEDVFRHYA GSDLEGGADP RRSGLGEVRS TRRTARRVS
NT Sequence
ATGACCGACC ACATGCCCGA ACCGACGCTT CCCGATCGAA CGCCGGCCAT CGACTGTGAC GGGCTGACCC ACCGCTACGG GCAGTTCACC GCGGTCGAGG ACCTGACGCT GCAGGTGCGC GCAGGCGAGA CCCTGGGCCT GCTCGGCCCC AACGGCGCGG GCAAGACCAC CGCGGTACGG GTCCTGACGA CGCTGACCCC GGTGCAGCAG GGCCGGGTGC GGATTTTCGG GCTCGATACG CGTGCGCACA CCATGGACAT CCGGCACAAC ATCGGCTATG TCCCGCAGCA GCTCTCGATC GAGGCGGCGC TGACCGGCAG GCAGAACGTC GAGTTGTTCG CGCGGCTGTA CGACGTGCCG CGGTCCGAGC GCGCAGACCG GGTGGCCGCC GCGCTCGAGG CCATGCAGTT GCTCGACGTC GCCGACACCG TCGCGGGCAC GTATTCGGGC GGCATGGTGC GCCGCCTCGA GTTGGCGCAG GCCCTGGTGA ACCGGCCCCT GCTGCTGATC CTCGACGAGC CGACCGTCGG CCTGGATCCC ATCGCGCGCG ACAGCGTGTG GACCCAGGTG CGCGCCATGC AGGCCGAGTT CGGCATGACA GTCCTGCTCA CCACGCACTA CATGGGCGAG GCCGATGCGT TGTGCGACCG CGTCGCGCTC ATGCACCACG GCCGGTTGCA GGCCGTCGGC TCGCCCGACG AGCTCAAGCG GGCCGTATCG AGCCAGGCAT CGGACGACGC CGGGGAGACC ACGCTCGAAG ACGTGTTCCG CCACTACGCG GGCTCGGATC TCGAGGGCGG CGCCGACCCG CGCCGCTCCG GTCTGGGTGA GGTCCGCTCG ACCAGGAGGA CGGCGCGCCG TGTCAGT
Details for MysmA.01124.a.A1.GE28877
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 33kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHMPEPT LPDRTPAIDC DGLTHRYGQF TAVEDLTLQV RAGETLGLLG PNGAGKTTAV RVLTTLTPVQ QGRVRIFGLD TRAHTMDIRH NIGYVPQQLS IEAALTGRQN VELFARLYDV PRSERADRVA AALEAMQLLD VADTVAGTYS GGMVRRLELA QALVNRPLLL ILDEPTVGLD PIARDSVWTQ VRAMQAEFGM TVLLTTHYMG EADALCDRVA LMHHGRLQAV GSPDELKRAV SSQXSXDAXE TTLEDVFRXY AXXISRXAPX RAAPXXXRXX XXGGRXXVSV NKXXETXFCX XXXXXXXXLT XPKRXXXXLX XXLXXXX
Validated NT Sequence
annnattttn nttnncttta aganggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaccac atgcccgaac cgacgcttcc cgatcgaacg ccggccatcg actgtgacgg gctgacccac cgctacgggc agttcaccgc ggtcgaggac ctgacgctgc aggtgcgcgc aggcgagacc ctgggcctgc tcggccccaa cggcgcgggc aagaccaccg cggtacgggt cctgacgacg ctgaccccgg tgcagcaggg ccgggtgcgg attttcgggc tcgatacgcg tgcgcacacc atggacatcc ggcacaacat cggctatgtc ccgcagcagc tctcgatcga ggcggcgctg accggcaggc agaacgtcga gttgttcgcg cggctgtacg acgtgccgcg gtccgagcgc gcagaccggg tggccgccgc gctcgaggcc atgcagttgc tcgacgtcgc cgacaccgtc gcgggcacgt attcgggcgg catggtgcgc cgcctcgagt tggcgcaggc cctggtgaac cggcccctgc tgctgatcct cgacgagccg accgtcggcc tggatcccat cgcgcgcgac agcgtgtgga cccaggtgcg cgccatgcag gccgagttcg gcatgacagt cctgctcacc acgcactaca tgggcgaggc cgatgcgttg tgcgaccgcg tcgcgctcat gcaccacggc cggttgcagg ccgtcggctc gcccgacgag ctcaagcggg ccgtatcgag ccaggnatcg gangacgccn gggagaccac gctcgaagac gtgttccgcc nctacgcggn tcnnatctcg agggnngcgc cnanccgcgc cgctccngnn nnggngaggn ncnctcnanc aggaggacgn ngngncgtgt cagttaataa acannncgaa acaannttct gcnncnnagn ntncnnnann nnccngntgc taacaaancc naaaaggann ntgannnngc tgnngncnnc gctnnnnnna nnannna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDHMPEPT LPDRTPAIDC DGLTHRYGQF TAVEDLTLQV RAGETLGLLG PNGAGKTTAV RVLTTLTPVQ QGRVRIFGLD TRAHTMDIRH NIGYVPQQLS IEAALTGRQN VELFARLYDV PRSERADRVA AALEAMQLLD VADTVAGTYS GGMVRRLELA QALVNRPLLL ILDEPTVGLD PIARDSVWTQ VRAMQAEFGM TVLLTTHYMG EADALCDRVA LMHHGRLQAV GSPDELKRAV SSQASDDAGE TTLEDVFRHY AGSDLEGGAD PRRSGLGEVR STRRTARRVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaccac atgcccgaac cgacgcttcc cgatcgaacg ccggccatcg actgtgacgg gctgacccac cgctacgggc agttcaccgc ggtcgaggac ctgacgctgc aggtgcgcgc aggcgagacc ctgggcctgc tcggccccaa cggcgcgggc aagaccaccg cggtacgggt cctgacgacg ctgaccccgg tgcagcaggg ccgggtgcgg attttcgggc tcgatacgcg tgcgcacacc atggacatcc ggcacaacat cggctatgtc ccgcagcagc tctcgatcga ggcggcgctg accggcaggc agaacgtcga gttgttcgcg cggctgtacg acgtgccgcg gtccgagcgc gcagaccggg tggccgccgc gctcgaggcc atgcagttgc tcgacgtcgc cgacaccgtc gcgggcacgt attcgggcgg catggtgcgc cgcctcgagt tggcgcaggc cctggtgaac cggcccctgc tgctgatcct cgacgagccg accgtcggcc tggatcccat cgcgcgcgac agcgtgtgga cccaggtgcg cgccatgcag gccgagttcg gcatgacagt cctgctcacc acgcactaca tgggcgaggc cgatgcgttg tgcgaccgcg tcgcgctcat gcaccacggc cggttgcagg ccgtcggctc gcccgacgag ctcaagcggg ccgtatcgag ccaggcatcg gacgacgccg gggagaccac gctcgaagac gtgttccgcc actacgcggg ctcggatctc gagggcggcg ccgacccgcg ccgctccggt ctgggtgagg tccgctcgac caggaggacg gcgcgccgtg tcagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa