SSGCID: Target Page

MysmA.01097.e

Short-chain dehydrogenase/reductase SDR

CENTER ID:	MysmA.01097.e
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01097.e.A1.GE28871	Full length( MysmA.01097.e )		1	263

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.2834
RefSeq:	YP_887192.1
UniProt:	A0QWA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRIADRVFVV TGAGNGMGRE VALGLARRGA HVAAVDLDEA GLAGTAERAR GTGARMSTHV LNVTDRDAVA GLPDAVLDVH GQIDGLVNIA GIAQRFALFT DLEADQIDRV TTVNFLGTVQ MCRAFLPVLR QRPEANITNM SSLSALVPFA SQVLYSASKA AVQQFSEGLD AELADTNVRV VTVFPGQVST NLAQNSGVKM LDAGGRRAPV TTPEAAGRKI VTGIAKDRYR VIIGTDAHVL YTLSRLAPRR TARMVAKQIK SVL

NT Sequence

ATGCGCATCG CGGATAGGGT TTTCGTCGTC ACCGGTGCAG GCAACGGCAT GGGGCGTGAG GTGGCGTTGG GCCTGGCCCG GCGGGGCGCA CACGTCGCCG CCGTCGACCT CGACGAAGCG GGCCTCGCCG GCACGGCCGA GCGCGCCCGC GGCACCGGGG CGAGGATGTC GACCCACGTT CTGAACGTCA CCGACCGAGA CGCGGTGGCC GGGCTGCCCG ACGCGGTGCT CGACGTCCAC GGCCAGATCG ACGGTCTGGT GAACATCGCC GGAATCGCCC AGAGGTTCGC GCTGTTCACC GACCTGGAGG CCGACCAGAT CGACCGGGTG ACGACCGTGA ACTTTTTGGG CACGGTGCAG ATGTGCCGAG CCTTCCTGCC GGTCCTTCGG CAACGACCCG AGGCCAACAT CACCAACATG TCCAGCCTGT CGGCGCTCGT GCCGTTCGCC AGCCAGGTGC TCTACAGCGC GAGCAAGGCC GCCGTCCAGC AGTTCAGCGA GGGACTCGAC GCCGAACTCG CCGACACCAA CGTCCGTGTG GTGACAGTGT TTCCGGGCCA GGTCTCCACC AACCTGGCAC AGAACTCCGG AGTGAAGATG CTCGACGCCG GCGGGCGGCG GGCCCCGGTC ACCACACCCG AGGCCGCCGG GCGCAAGATC GTCACCGGCA TCGCCAAGGA CCGGTACCGG GTGATCATCG GTACCGACGC CCACGTGCTG TACACACTGT CGCGGTTGGC GCCGCGGCGC ACGGCCCGGA TGGTGGCCAA ACAGATCAAA TCCGTTCTC

Details for MysmA.01097.e.A1.GE28871

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRIADRVFV VTGAGNGMGR EVALGLARRG AHVAAVDLDE AGLAGTAERA RGTGARMSTH VLNVTDRDAV AGLPDAVLDV HGQIDGLVNI AGIAQRFALF TDLEADQIDR VTTVNFLGTV QMCRAFLPVL RQRPEANITN MSSLSALVPF ASQVLYSASK AAVQQFSEGL DAELADTNVR VVTVFPGQVS TNLAQNSGVK MLDAGGRRAP VTTPEAAGRK IVTGIAKDRY RVIIGTDAHV LYTLSRLAPR RTARMVAKQI KSVL

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgca tcgcggatag ggttttcgtc gtcaccggtg caggcaacgg catggggcgt gaggtggcgt tgggcctggc ccggcggggc gcacacgtcg ccgccgtcga cctcgacgaa gcgggcctcg ccggcacggc cgagcgcgcc cgcggcaccg gggcgaggat gtcgacccac gttctgaacg tcaccgaccg agacgcggtg gccgggctgc ccgacgcggt gctcgacgtc cacggccaga tcgacggtct ggtgaacatc gccggaatcg cccagaggtt cgcgctgttc accgacctgg aggccgacca gatcgaccgg gtgacgaccg tgaacttttt gggcacggtg cagatgtgcc gagccttcct gccggtcctt cggcaacgac ccgaggccaa catcaccaac atgtccagcc tgtcggcgct cgtgccgttc gccagccagg tgctctacag cgcgagcaag gccgccgtcc agcagttcag cgagggactc gacgccgaac tcgccgacac caacgtccgt gtggtgacag tgtttccggg ccaggtctcc accaacctgg cacagaactc cggagtgaag atgctcgacg ccggcgggcg gcgggccccg gtcaccacac ccgaggccgc cgggcgcaag atcgtcaccg gcatcgccaa ggaccggtac cgggtgatca tcggtaccga cgcccacgtg ctgtacacac tgtcgcggtt ggcgccgcgg cgcacggccc ggatggtggc caaacagatc aaatccgttc tctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcatcgcg gatagggttt tcgtcgtcac cggtgcaggc aacggcatgg ggcgtgaggt ggcgttgggc ctggcccggc ggggcgcaca cgtcgccgcc gtcgacctcg acgaagcggg cctcgccggc acggccgagc gcgcccgcgg caccggggcg aggatgtcga cccacgttct gaacgtcacc gaccgagacg cggtggccgg gctgcccgac gcggtgctcg acgtccacgg ccagatcgac ggtctggtga acatcgccgg aatcgcccag aggttcgcgc tgttcaccga cctggaggcc gaccagatcg accgggtgac gaccgtgaac tttttgggca cggtgcagat gtgccgagcc ttcctgccgg tccttcggca acgacccgag gccaacatca ccaacatgtc cagcctgtcg gcgctcgtgc cgttcgccag ccaggtgctc tacagcgcga gcaaggccgc cgtccagcag ttcagcgagg gactcgacgc cgaactcgcc gacaccaacg tccgtgtggt gacagtgttt ccgggccagg tctccaccaa cctggcacag aactccggag tgaagatgct cgacgccggc gggcggcggg ccccggtcac cacacccgag gccgccgggc gcaagatcgt caccggcatc gccaaggacc ggtaccgggt gatcatcggt accgacgccc acgtgctgta cacactgtcg cggttggcgc cgcggcgcac ggcccggatg gtggccaaac agatcaaatc cgttctcaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa