SSGCID: Target Page

MysmA.01097.c

Short chain alcohol dehydrogenase

CENTER ID:	MysmA.01097.c
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01097.c.A1.GE28869	Full length( MysmA.01097.c )		1	274

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.4931
RefSeq:	YP_889303.1
UniProt:	A0R2B5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEGFAGKVAV VTGAGSGIGQ ALAIELGRSG AKLAICDVDT EGLAATEERL KAIGAPVKAD RLDVTEREAF LLYADAVKEH YGTVNQIYNN AGIAFTGDVE ISEFKDIERV MDVDYWGVVN GTKAFLPHLI ASGDGHVINV SSVFGLFSVP GQAAYNSAKF AVRGFTEALR QEMAIAKHPV KVTTVHPGGI KTAIARNATA AEGLDAKELA EAFDKKLANT TPQRAAVIIL DGVRKNKARV LVGPDAKILD VIVRITGSGY QRLFSAVAAK AMPR

NT Sequence

ATGGAGGGCT TCGCCGGGAA AGTCGCCGTC GTCACGGGCG CCGGTAGCGG CATCGGGCAG GCACTGGCCA TCGAACTGGG CCGCTCGGGC GCCAAGCTGG CCATCTGCGA CGTCGACACC GAAGGCCTCG CCGCGACCGA GGAACGTCTC AAGGCCATCG GCGCCCCCGT GAAGGCCGAC CGCCTCGACG TCACCGAACG CGAGGCGTTC CTGCTCTACG CCGACGCGGT CAAGGAGCAC TACGGCACGG TCAACCAGAT CTACAACAAC GCGGGCATCG CGTTCACCGG TGACGTCGAG ATCAGCGAAT TCAAGGACAT CGAGCGCGTG ATGGACGTCG ACTACTGGGG CGTGGTCAAC GGCACCAAGG CGTTCCTGCC GCATCTGATC GCCTCGGGCG ACGGGCACGT GATCAACGTG TCGAGCGTCT TCGGGCTTTT CTCGGTGCCC GGCCAGGCCG CGTACAACTC GGCCAAGTTC GCGGTGCGCG GGTTCACCGA GGCGCTGCGC CAGGAGATGG CGATCGCCAA GCATCCCGTC AAGGTCACCA CGGTGCACCC GGGCGGCATC AAGACCGCGA TCGCCCGCAA CGCCACCGCG GCCGAGGGAC TCGACGCCAA GGAACTCGCC GAGGCGTTCG ACAAGAAGCT CGCCAACACC ACGCCTCAGC GCGCGGCGGT GATCATCCTC GACGGCGTGC GCAAGAACAA GGCGCGCGTA CTCGTGGGCC CCGACGCCAA GATCCTCGAC GTCATCGTCC GGATCACCGG ATCGGGTTAC CAGCGGCTGT TCTCGGCCGT CGCGGCCAAG GCCATGCCCC GC

Details for MysmA.01097.c.A1.GE28869

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	31kDa
OBSERVED MW:	31kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMEGFAGKVA VVTGAGSGIG QALAIELGRS GAKLAICDVD TEGLAATEER LKAIGAPVKA DRLDVTEREA FLLYADAVKE HYGTVNQIYN NAGIAFTGDV EISEFKDIER VMDVDYWGVV NGTKAFLPHL IASGDGHVIN VSSVFGLFSV PGQAAYNSAK FAVRGFTEAL RQEMAIAKHP VKVTTVHPGG IKTAIARNAT AAEGLDAKEL AEAFDKKLAN TTPQRAAVII LDGVRKNKAR VLVGPDAKIL DVIVRITGSG YQRLFSAVAA KAMPR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggagg gcttcgccgg gaaagtcgcc gtcgtcacgg gcgccggtag cggcatcggg caggcactgg ccatcgaact gggccgctcg ggcgccaagc tggccatctg cgacgtcgac accgaaggcc tcgccgcgac cgaggaacgt ctcaaggcca tcggcgcccc cgtgaaggcc gaccgcctcg acgtcaccga acgcgaggcg ttcctgctct acgccgacgc ggtcaaggag cactacggca cggtcaacca gatctacaac aacgcgggca tcgcgttcac cggtgacgtc gagatcagcg aattcaagga catcgagcgc gtgatggacg tcgactactg gggcgtggtc aacggcacca aggcgttcct gccgcatctg atcgcctcgg gcgacgggca cgtgatcaac gtgtcgagcg tcttcgggct tttctcggtg cccggccagg ccgcgtacaa ctcggccaag ttcgcggtgc gcgggttcac cgaggcgctg cgccaggaga tggcgatcgc caagcatccc gtcaaggtca ccacggtgca cccgggcggc atcaagaccg cgatcgcccg caacgccacc gcggccgagg gactcgacgc caaggaactc gccgaggcgt tcgacaagaa gctcgccaac accacgcctc agcgcgcggc ggtgatcatc ctcgacggcg tgcgcaagaa caaggcgcgc gtactcgtgg gccccgacgc caagatcctc gacgtcatcg tccggatcac cggatcgggt taccagcggc tgttctcggc cgtcgcggcc aaggccatgc cccgctaata a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagggcttc gccgggaaag tcgccgtcgt cacgggcgcc ggtagcggca tcgggcaggc actggccatc gaactgggcc gctcgggcgc caagctggcc atctgcgacg tcgacaccga aggcctcgcc gcgaccgagg aacgtctcaa ggccatcggc gcccccgtga aggccgaccg cctcgacgtc accgaacgcg aggcgttcct gctctacgcc gacgcggtca aggagcacta cggcacggtc aaccagatct acaacaacgc gggcatcgcg ttcaccggtg acgtcgagat cagcgaattc aaggacatcg agcgcgtgat ggacgtcgac tactggggcg tggtcaacgg caccaaggcg ttcctgccgc atctgatcgc ctcgggcgac gggcacgtga tcaacgtgtc gagcgtcttc gggcttttct cggtgcccgg ccaggccgcg tacaactcgg ccaagttcgc ggtgcgcggg ttcaccgagg cgctgcgcca ggagatggcg atcgccaagc atcccgtcaa ggtcaccacg gtgcacccgg gcggcatcaa gaccgcgatc gcccgcaacg ccaccgcggc cgagggactc gacgccaagg aactcgccga ggcgttcgac aagaagctcg ccaacaccac gcctcagcgc gcggcggtga tcatcctcga cggcgtgcgc aagaacaagg cgcgcgtact cgtgggcccc gacgccaaga tcctcgacgt catcgtccgg atcaccggat cgggttacca gcggctgttc tcggccgtcg cggccaaggc catgccccgc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa