SSGCID: Target Page

MysmA.01097.a

Oxidoreductase, short chain dehydrogenase/reductase family protein

CENTER ID:	MysmA.01097.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01097.a.A1.GE28867	Full length( MysmA.01097.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3744
RefSeq:	YP_888099.1
UniProt:	A0QYW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPSLATESDH SVIFVGFEGE IMKLHGATAL VTGANRGLGH HLAVELIRRG AKVYATARRP ELVDVPGAHV LRLDITDQWS VEAAAAAADD VDVLINNAAF TDGGNLVTGD LAKIRATMDS NYYGTLAMIR AFAPILARNG GGAILNVLSV AAWRTVDGNT SYAAAKSAEW GLTNGVRVEL ARQGTHVAAL VPGLIRTDTL LEFARGNGIE LPEEHMNEPA DLARMALDGL EAGEVEIIDR MSVDAKAALA GPPVTLSL

NT Sequence

ATGCCTAGTC TGGCGACGGA AAGTGACCAC TCGGTCATCT TCGTCGGCTT CGAAGGGGAG ATCATGAAAC TGCACGGCGC CACCGCACTC GTCACGGGTG CCAACCGGGG CCTCGGGCAC CACCTCGCGG TCGAACTGAT CCGCCGCGGT GCCAAGGTGT ACGCCACCGC GCGGCGCCCT GAACTCGTCG ATGTCCCGGG TGCGCACGTG CTGCGGCTCG ACATCACCGA TCAGTGGTCC GTCGAAGCCG CCGCCGCGGC GGCCGATGAC GTCGACGTGC TGATCAACAA CGCGGCATTC ACCGACGGCG GCAACCTCGT CACGGGTGAC CTCGCCAAGA TCCGGGCGAC GATGGATTCG AACTACTACG GCACGCTGGC CATGATCCGC GCGTTCGCGC CGATCCTGGC CCGCAACGGC GGGGGAGCCA TTCTCAACGT GCTGTCCGTT GCGGCGTGGA GAACCGTCGA CGGCAACACG TCCTATGCCG CCGCGAAATC CGCGGAATGG GGGTTGACCA ACGGTGTTCG CGTGGAACTC GCGCGCCAGG GCACCCACGT CGCCGCTCTG GTACCCGGGC TGATCCGCAC CGACACGCTG CTCGAGTTCG CCCGCGGCAA CGGGATCGAA CTGCCCGAGG AACACATGAA CGAACCCGCC GACCTCGCCC GGATGGCCCT CGACGGGCTC GAAGCCGGTG AGGTCGAGAT CATCGATCGG ATGAGCGTGG ACGCGAAAGC TGCTCTGGCC GGCCCGCCGG TCACGCTCAG CCTG

Details for MysmA.01097.a.A1.GE28867

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMPSLATESD HSVIFVGFEG EIMKLHGATA LVTGANRGLG HHLAVELIRR GAKVYATARR PELVDVPGAH VLRLDITDQW SVEAAAAAAD DVDVLINNAA FTDGGNLVTG DLAKIRATMD SNYYGTLAMI RAFAPILARN GGGAILNVLS VAAWRTVDGN TSYAAAKSAE WGLTNGVRVE LARQGTHVAA LVPGLIRTDT LLEFARGNGI ELPEEHMNEP ADLARMALDG LEAGEVEIID RMSVDAKAAL AGPPVTLS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgccta gtctggcgac ggaaagtgac cactcggtca tcttcgtcgg cttcgaaggg gagatcatga aactgcacgg cgccaccgca ctcgtcacgg gtgccaaccg gggcctcggg caccacctcg cggtcgaact gatccgccgc ggtgccaagg tgtacgccac cgcgcggcgc cctgaactcg tcgatgtccc gggtgcgcac gtgctgcggc tcgacatcac cgatcagtgg tccgtcgaag ccgccgccgc ggcggccgat gacgtcgacg tgctgatcaa caacgcggca ttcaccgacg gcggcaacct cgtcacgggt gacctcgcca agatccgggc gacgatggat tcgaactact acggcacgct ggccatgatc cgcgcgttcg cgccgatcct ggcccgcaac ggcgggggag ccattctcaa cgtgctgtcc gttgcggcgt ggagaaccgt cgacggcaac acgtcctatg ccgccgcgaa atccgcggaa tgggggttga ccaacggtgt tcgcgtggaa ctcgcgcgcc agggcaccca cgtcgccgct ctggtacccg ggctgatccg caccgacacg ctgctcgagt tcgcccgcgg caacgggatc gaactgcccg aggaacacat gaacgaaccc gccgacctcg cccggatggc cctcgacggg ctcgaagccg gtgaggtcga gatcatcgat cggatgagcg tggacgcgaa agctgctctg gccggcccgc cggtcacgct cagcct

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctagtctg gcgacggaaa gtgaccactc ggtcatcttc gtcggcttcg aaggggagat catgaaactg cacggcgcca ccgcactcgt cacgggtgcc aaccggggcc tcgggcacca cctcgcggtc gaactgatcc gccgcggtgc caaggtgtac gccaccgcgc ggcgccctga actcgtcgat gtcccgggtg cgcacgtgct gcggctcgac atcaccgatc agtggtccgt cgaagccgcc gccgcggcgg ccgatgacgt cgacgtgctg atcaacaacg cggcattcac cgacggcggc aacctcgtca cgggtgacct cgccaagatc cgggcgacga tggattcgaa ctactacggc acgctggcca tgatccgcgc gttcgcgccg atcctggccc gcaacggcgg gggagccatt ctcaacgtgc tgtccgttgc ggcgtggaga accgtcgacg gcaacacgtc ctatgccgcc gcgaaatccg cggaatgggg gttgaccaac ggtgttcgcg tggaactcgc gcgccagggc acccacgtcg ccgctctggt acccgggctg atccgcaccg acacgctgct cgagttcgcc cgcggcaacg ggatcgaact gcccgaggaa cacatgaacg aacccgccga cctcgcccgg atggccctcg acgggctcga agccggtgag gtcgagatca tcgatcggat gagcgtggac gcgaaagctg ctctggccgg cccgccggtc acgctcagcc tgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa