MysmA.01062.a

Dihydrofolate reductase

CENTER ID: MysmA.01062.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: in PDB
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.01062.a.B1.GE40213 Full length(MysmA.01062.a) 1 156
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

6CXM
DEPOSITED: 4/3/2018
DETERMINATION: XRay
CLONE: MysmA.01062.a.B1.GE40213
PROTEIN: MysmA.01062.a.B1.PS38260
7L9T
DEPOSITED: 1/5/2021
DETERMINATION: XRay
CLONE: MysmA.01062.a.B1.GE40213
PROTEIN: MysmA.01062.a.B1.PS38260

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.56.peg.2671
UniProt: I7FC10

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSMRLIWAQS TSGIIGRDNS IPWRLPEDLA RFKEMTMGHP VVMGRLTWES LPASVRPLPG RRNIVVTRDA DYRAEGAEVV TDLPDEPDAW VIGGAQIYAM ALARADRCEV TEVDIALTPL DGDARAPVLD DSWVATTGEW QTSTSGLRFR FCSYRR
NT Sequence
ATGAGTATGA GACTGATCTG GGCCCAGTCG ACCTCAGGGA TCATCGGCCG GGACAACTCC ATTCCATGGC GTCTGCCCGA GGACCTCGCC CGGTTCAAGG AGATGACCAT GGGCCATCCG GTGGTGATGG GACGGCTCAC GTGGGAGTCG CTGCCTGCCT CGGTGCGCCC GCTGCCGGGG CGCCGCAACA TCGTCGTGAC CCGTGACGCC GACTACCGGG CCGAGGGCGC CGAGGTGGTC ACCGACCTGC CCGACGAACC CGATGCGTGG GTGATCGGCG GCGCCCAGAT CTATGCGATG GCACTGGCGC GGGCCGACCG CTGTGAGGTC ACCGAGGTCG ACATCGCCCT GACGCCACTC GACGGTGATG CGCGCGCACC GGTGCTCGAC GACTCGTGGG TCGCGACGAC GGGTGAGTGG CAGACCAGTA CGTCAGGCCT GCGTTTCCGG TTCTGCAGCT ACCGGCGC
Details for MysmA.01062.a.B1.GE40213
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 18kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS MRLIWAQSTS GIIGRDNSIP WRLPEDLARF KEMTMGHPVV MGRLTWESLP ASVRPLPGRR NIVVTRDADY RAEGAEVVTD LPDEPDAWVI GGAQIYAMAL ARADRCEVTE VDIALTPLDG DARAPVLDDS WVATTGEWQT STSGLRFRFC SYRR
Validated NT Sequence
atcttactca cttagcgccg gtagctgcag aaccggaaac gcaggcctga cgtactggtc tgccactcac ccgtcgtcgc gacccacgag tcgtcgagca ccggtgcgcg cgcatcaccg tcgagtggcg tcagggcgat gtcgacctcg gtgacctcac agcggtcggc ccgcgccagt gccatcgcat agatctgggc gccgccgatc acccacgcat cgggttcgtc gggcaggtcg gtgaccacct cggcgccctc ggcccggtag tcggcgtcac gggtcacgac gatgttgcgg cgccccggca gcgggcgcac cgaggcaggc agcgactccc acgtgagccg tcccatcacc accggatggc ccatggtcat ctccttgaac cgggcgaggt cctcgggcag acgccatgga atggagttgt cccggccgat gatccctgag gtcgactggg cccagatcag tctcatactc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS MRLIWAQSTS GIIGRDNSIP WRLPEDLARF KEMTMGHPVV MGRLTWESLP ASVRPLPGRR NIVVTRDADY RAEGAEVVTD LPDEPDAWVI GGAQIYAMAL ARADRCEVTE VDIALTPLDG DARAPVLDDS WVATTGEWQT STSGLRFRFC SYRR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagta tgagactgat ctgggcccag tcgacctcag ggatcatcgg ccgggacaac tccattccat ggcgtctgcc cgaggacctc gcccggttca aggagatgac catgggccat ccggtggtga tgggacggct cacgtgggag tcgctgcctg cctcggtgcg cccgctgccg gggcgccgca acatcgtcgt gacccgtgac gccgactacc gggccgaggg cgccgaggtg gtcaccgacc tgcccgacga acccgatgcg tgggtgatcg gcggcgccca gatctatgcg atggcactgg cgcgggccga ccgctgtgag gtcaccgagg tcgacatcgc cctgacgcca ctcgacggtg atgcgcgcgc accggtgctc gacgactcgt gggtcgcgac gacgggtgag tggcagacca gtacgtcagg cctgcgtttc cggttctgca gctaccggcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat