MysmA.01019.b
Dihydropteroate synthase
| CENTER ID: | MysmA.01019.b |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.01019.b.B1.GE40212 | Full length(MysmA.01019.b) | 1 | 285 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6OMZ
DEPOSITED: 4/19/2019
DETERMINATION: XRay
CLONE: MysmA.01019.b.B1.GE40212
PROTEIN: MysmA.01019.b.B1.PW38386
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|246196.19.peg.5942 |
| UniProt: | A0R586 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNPPSLTAQP GTPVQVMGVV NVTQDSFSDG GKFIDTDRAV EHGLALVAAG AQIIDVGGES TRPGATRIDP GVEAARVTPV IRELAAQGIT ISIDTMHADV ARAALEAGAH IVNDVSGGRA DPGMAGVLAE AKVPWILMHW RSVDADHPHR VPGYRDVVAE VRTELLAAVD AAVTAGVEPE RLVIDPGLGF AKTAEHNWAL LHALPDLVAT GVPVLVGASR KRFLGTLLAA ADGTPRPPDG RETATAVISV LAAMHGAWGV RVHDVQASVD ALKVLGAWTS GGQIG
NT Sequence
GTGAACCCAC CGAGCCTGAC GGCCCAGCCC GGCACACCCG TGCAGGTGAT GGGCGTCGTG AACGTCACGC AAGACTCGTT CTCCGACGGT GGGAAATTCA TCGACACCGA CCGCGCCGTC GAGCACGGGC TGGCCCTGGT GGCCGCGGGC GCACAGATCA TCGACGTCGG CGGTGAGTCC ACGCGGCCCG GCGCGACCCG CATCGATCCC GGGGTCGAGG CCGCCCGCGT CACGCCGGTG ATCAGAGAGC TTGCGGCCCA AGGCATCACG ATCAGCATCG ACACCATGCA CGCCGACGTC GCGCGCGCGG CGCTCGAAGC GGGCGCGCAC ATCGTCAACG ACGTGTCGGG CGGGCGGGCC GATCCCGGTA TGGCCGGCGT GCTCGCCGAG GCGAAGGTGC CGTGGATCCT CATGCACTGG CGTTCGGTCG ACGCCGACCA CCCGCACCGC GTTCCGGGAT ACCGCGACGT GGTCGCCGAG GTGCGCACCG AACTGCTCGC GGCGGTCGAC GCCGCGGTGA CCGCGGGCGT CGAACCGGAG CGGCTCGTCA TCGATCCCGG CCTGGGTTTC GCCAAGACCG CCGAGCACAA CTGGGCGCTG CTGCATGCGC TGCCGGACCT GGTGGCCACG GGCGTCCCGG TGCTGGTTGG CGCGTCGCGC AAACGTTTCC TCGGTACGCT GCTGGCCGCC GCGGACGGCA CACCGCGCCC ACCGGACGGC CGTGAGACCG CGACCGCGGT GATCTCGGTG CTGGCCGCGA TGCACGGTGC CTGGGGCGTG CGGGTGCACG ACGTACAGGC CTCGGTCGAC GCATTGAAGG TGCTGGGCGC CTGGACTTCT GGAGGACAGA TTGGC
Details for MysmA.01019.b.B1.GE40212
| HARVESTED ON: | 5/9/2016 |
| SEQUENCED ON: | 5/23/2016 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV NPPSLTAQPG TPVQVMGVVN VTQDSFSDGG KFIDTDRAVE HGLALVAAGA QIIDVGGEST RPGATRIDPG VEAARVTPVI RELAAQGITI SIDTMHADVA RAALEAGAHI VNDVSGGRAD PGMAGVLAEA KVPWILMHWR SVDADHPHRV PGYRDVVAEV RTELLAAVDA AVTAGVEPER LVIDPGLGFA KTAEHNWALL HALPDLVATG VPVLVGASRK RFLGTLLAAA DGTPRPPDGR ETATAVISVL AAMHGAWGVR VHDVQASVDA LKVLGXXXXG EQIG
Validated NT Sequence
ttactcactt agccaatctg ttctccagnn nnnnnnncgc ccagcacctt caatgcgtcg accgaggcct gtacgtcgtg cacccgcacg ccccaggcac cgtgcatcgc ggccagcacc gagatcaccg cggtcgcggt ctcacggccg tccggtgggc gcggtgtgcc gtccgcggcg gccagcagcg taccgaggaa acgtttgcgc gacgcgccaa ccagcaccgg gacgcccgtg gccaccaggt ccggcagcgc atgcagcagc gcccagttgt gctcggcggt cttggcgaaa cccaggccgg gatcgatgac gagccgctcc ggttcgacgc ccgcggtcac cgcggcgtcg accgccgcga gcagttcggt gcgcacctcg gcgaccacgt cgcggtatcc cggaacgcgg tgcgggtggt cggcgtcgac cgaacgccag tgcatgagga tccacggcac cttcgcctcg gcgagcacgc cggccatacc gggatcggcc cgcccgcccg acacgtcgtt gacgatgtgc gcgcccgctt cgagcgccgc gcgcgcgacg tcggcgtgca tggtgtcgat gctgatcgtg atgccttggg ccgcaagctc tctgatcacc ggcgtgacgc gggcggcctc gaccccggga tcgatgcggg tcgcgccggg ccgcgtggac tcaccgccga cgtcgatgat ctgtgcgccc gcggccacca gggccagccc gtgctcgacg gcgcggtcgg tgtcgatgaa tttcccaccg tcggagaacg agtcttgcgt gacgttcacg acgcccatca cctgcacggg tgtgccgggc tgggccgtca ggctcggtgg gttcaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMN PPSLTAQPGT PVQVMGVVNV TQDSFSDGGK FIDTDRAVEH GLALVAAGAQ IIDVGGESTR PGATRIDPGV EAARVTPVIR ELAAQGITIS IDTMHADVAR AALEAGAHIV NDVSGGRADP GMAGVLAEAK VPWILMHWRS VDADHPHRVP GYRDVVAEVR TELLAAVDAA VTAGVEPERL VIDPGLGFAK TAEHNWALLH ALPDLVATGV PVLVGASRKR FLGTLLAAAD GTPRPPDGRE TATAVISVLA AMHGAWGVRV HDVQASVDAL KVLGAWTSGG QIG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaacc caccgagcct gacggcccag cccggcacac ccgtgcaggt gatgggcgtc gtgaacgtca cgcaagactc gttctccgac ggtgggaaat tcatcgacac cgaccgcgcc gtcgagcacg ggctggccct ggtggccgcg ggcgcacaga tcatcgacgt cggcggtgag tccacgcggc ccggcgcgac ccgcatcgat cccggggtcg aggccgcccg cgtcacgccg gtgatcagag agcttgcggc ccaaggcatc acgatcagca tcgacaccat gcacgccgac gtcgcgcgcg cggcgctcga agcgggcgcg cacatcgtca acgacgtgtc gggcgggcgg gccgatcccg gtatggccgg cgtgctcgcc gaggcgaagg tgccgtggat cctcatgcac tggcgttcgg tcgacgccga ccacccgcac cgcgttccgg gataccgcga cgtggtcgcc gaggtgcgca ccgaactgct cgcggcggtc gacgccgcgg tgaccgcggg cgtcgaaccg gagcggctcg tcatcgatcc cggcctgggt ttcgccaaga ccgccgagca caactgggcg ctgctgcatg cgctgccgga cctggtggcc acgggcgtcc cggtgctggt tggcgcgtcg cgcaaacgtt tcctcggtac gctgctggcc gccgcggacg gcacaccgcg cccaccggac ggccgtgaga ccgcgaccgc ggtgatctcg gtgctggccg cgatgcacgg tgcctggggc gtgcgggtgc acgacgtaca ggcctcggtc gacgcattga aggtgctggg cgcctggact tctggaggac agattggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat