SSGCID: Target Page

MysmA.01019.a

Dihydropteroate synthase

CENTER ID:	MysmA.01019.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01019.a.B1.GE40211	Full length(MysmA.01019.a)		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.01019.a.B1.PW37985	Full length(MysmA.01019.a)		1	291

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.4963
UniProt:	A0R2E7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFSTFCGRPV AHDRALIMAI VNRTPDSFYD RGATFTDEAA KAAAHRVIDE GADVIDVGGV KAGPGSNVDA EEEITRVVPF IEWLRSTFPD RLISIDTWRA AVAKQACAAG ADIINDTWAG ADPGLAEVAA EFGAGLVCSH TGGATPRTRP FRVNYGISER GVVDDVIAEV TAAAERAVAT GVQRDRILID PTHDFGKNTY HGLSLLRHVK DLVNTGWPVL MALSNKDFVG ETLGVDLTER LEGTLAATAL AAADGAAMFR VHEVGPTRRV LEMVASIQGV RKPARTVRGL A

NT Sequence

GTGTTCTCGA CGTTCTGCGG TCGTCCGGTG GCGCACGACC GTGCGCTCAT CATGGCGATC GTCAACCGGA CACCCGATTC GTTCTACGAC CGCGGCGCCA CCTTCACCGA CGAGGCAGCC AAGGCCGCCG CGCACCGTGT GATCGACGAG GGTGCCGACG TCATCGACGT CGGCGGCGTC AAGGCGGGCC CCGGCAGCAA CGTCGACGCC GAAGAGGAGA TCACGCGCGT CGTCCCGTTC ATCGAGTGGC TGCGCAGCAC CTTCCCCGAC CGGTTGATCA GCATCGACAC GTGGCGCGCG GCCGTCGCCA AGCAGGCGTG CGCGGCGGGC GCGGACATCA TCAACGACAC CTGGGCCGGT GCCGACCCGG GTCTGGCCGA GGTGGCCGCC GAGTTCGGCG CGGGCCTGGT GTGCTCACAC ACGGGCGGCG CGACGCCTCG CACGCGGCCG TTCCGGGTGA ACTACGGCAT CAGCGAGCGC GGTGTGGTCG ACGACGTGAT CGCGGAGGTG ACCGCTGCCG CCGAGCGGGC CGTGGCAACG GGTGTTCAGC GCGACCGGAT CCTCATCGAT CCGACCCATG ATTTCGGCAA AAACACTTAT CACGGTCTTA GTTTGTTGCG CCACGTAAAA GATCTTGTTA ATACCGGATG GCCGGTCCTG ATGGCGCTGA GCAACAAGGA TTTTGTCGGG GAGACTCTCG GTGTGGACCT GACCGAACGC CTGGAAGGGA CGCTCGCCGC AACAGCGCTT GCCGCCGCGG ATGGAGCAGC CATGTTCCGG GTACACGAGG TGGGGCCCAC TCGGCGCGTG CTCGAAATGG TCGCGTCGAT TCAGGGAGTG CGGAAGCCCG CACGAACAGT GAGGGGACTG GCA

Details for MysmA.01019.a.B1.GE40211

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV FSTFCGRPVA HDRALIMAIV NRTPDSFYDR GATFTDEAAK AAAHRVIDEG ADVIDVGGVK AGPGSNVDAE EEITRVVPFI EWLRSTFPDR LISIDTWRAA VAKQACAAGA DIINDTWAGA DPGLAEVAAE FGAGLVCSHT GGATPRTRPF RVNYGISERG VVDDVIAEVT AAAERAVATG VQRDRILIDP THDFGKNTYH GLSLLRHVKD LVNTGWPVLM ALSNKDFVGE TLGVDLTERL EGTLAATALA AADGAAMFRV HEVGPTRRVL EMVASIQGVR KXXXTVRGLA

Validated NT Sequence

ttatgccagt cccctcactg ttnnnnnnng cttccgcact ccctgaatcg acgcgaccat ttcgagcacg cgccgagtgg gccccacctc gtgtacccgg aacatggctg ctccatccgc ggcggcaagc gctgttgcgg cgagcgtccc ttccaggcgt tcggtcaggt ccacaccgag agtctccccg acaaaatcct tgttgctcag cgccatcagg accggccatc cggtattaac aagatctttt acgtggcgca acaaactaag accgtgataa gtgtttttgc cgaaatcatg ggtcggatcg atgaggatcc ggtcgcgctg aacacccgtt gccacggccc gctcggcggc agcggtcacc tccgcgatca cgtcgtcgac cacaccgcgc tcgctgatgc cgtagttcac ccggaacggc cgcgtgcgag gcgtcgcgcc gcccgtgtgt gagcacacca ggcccgcgcc gaactcggcg gccacctcgg ccagacccgg gtcggcaccg gcccaggtgt cgttgatgat gtccgcgccc gccgcgcacg cctgcttggc gacggccgcg cgccacgtgt cgatgctgat caaccggtcg gggaaggtgc tgcgcagcca ctcgatgaac gggacgacgc gcgtgatctc ctcttcggcg tcgacgttgc tgccggggcc cgccttgacg ccgccgacgt cgatgacgtc ggcaccctcg tcgatcacac ggtgcgcggc ggccttggct gcctcgtcgg tgaaggtggc gccgcggtcg tagaacgaat cgggtgtccg gttgacgatc gccatgatga gcgcacggtc gtgcgccacc ggacgaccgc agaacgtcga gaacaccata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMF STFCGRPVAH DRALIMAIVN RTPDSFYDRG ATFTDEAAKA AAHRVIDEGA DVIDVGGVKA GPGSNVDAEE EITRVVPFIE WLRSTFPDRL ISIDTWRAAV AKQACAAGAD IINDTWAGAD PGLAEVAAEF GAGLVCSHTG GATPRTRPFR VNYGISERGV VDDVIAEVTA AAERAVATGV QRDRILIDPT HDFGKNTYHG LSLLRHVKDL VNTGWPVLMA LSNKDFVGET LGVDLTERLE GTLAATALAA ADGAAMFRVH EVGPTRRVLE MVASIQGVRK PARTVRGLA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgttct cgacgttctg cggtcgtccg gtggcgcacg accgtgcgct catcatggcg atcgtcaacc ggacacccga ttcgttctac gaccgcggcg ccaccttcac cgacgaggca gccaaggccg ccgcgcaccg tgtgatcgac gagggtgccg acgtcatcga cgtcggcggc gtcaaggcgg gccccggcag caacgtcgac gccgaagagg agatcacgcg cgtcgtcccg ttcatcgagt ggctgcgcag caccttcccc gaccggttga tcagcatcga cacgtggcgc gcggccgtcg ccaagcaggc gtgcgcggcg ggcgcggaca tcatcaacga cacctgggcc ggtgccgacc cgggtctggc cgaggtggcc gccgagttcg gcgcgggcct ggtgtgctca cacacgggcg gcgcgacgcc tcgcacgcgg ccgttccggg tgaactacgg catcagcgag cgcggtgtgg tcgacgacgt gatcgcggag gtgaccgctg ccgccgagcg ggccgtggca acgggtgttc agcgcgaccg gatcctcatc gatccgaccc atgatttcgg caaaaacact tatcacggtc ttagtttgtt gcgccacgta aaagatcttg ttaataccgg atggccggtc ctgatggcgc tgagcaacaa ggattttgtc ggggagactc tcggtgtgga cctgaccgaa cgcctggaag ggacgctcgc cgcaacagcg cttgccgccg cggatggagc agccatgttc cgggtacacg aggtggggcc cactcggcgc gtgctcgaaa tggtcgcgtc gattcaggga gtgcggaagc ccgcacgaac agtgagggga ctggcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for MysmA.01019.a.B1.PW37985

PURIFICATION DATe:	12/6/2016
CONCENTRATION:	31mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGTTCT CGACGTTCTG CGGTCGTCCG GTGGCGCACG ACCGTGCGCT CATCATGGCG ATCGTCAACC GGACACCCGA TTCGTTCTAC GACCGCGGCG CCACCTTCAC CGACGAGGCA GCCAAGGCCG CCGCGCACCG TGTGATCGAC GAGGGTGCCG ACGTCATCGA CGTCGGCGGC GTCAAGGCGG GCCCCGGCAG CAACGTCGAC GCCGAAGAGG AGATCACGCG CGTCGTCCCG TTCATCGAGT GGCTGCGCAG CACCTTCCCC GACCGGTTGA TCAGCATCGA CACGTGGCGC GCGGCCGTCG CCAAGCAGGC GTGCGCGGCG GGCGCGGACA TCATCAACGA CACCTGGGCC GGTGCCGACC CGGGTCTGGC CGAGGTGGCC GCCGAGTTCG GCGCGGGCCT GGTGTGCTCA CACACGGGCG GCGCGACGCC TCGCACGCGG CCGTTCCGGG TGAACTACGG CATCAGCGAG CGCGGTGTGG TCGACGACGT GATCGCGGAG GTGACCGCTG CCGCCGAGCG GGCCGTGGCA ACGGGTGTTC AGCGCGACCG GATCCTCATC GATCCGACCC ATGATTTCGG CAAAAACACT TATCACGGTC TTAGTTTGTT GCGCCACGTA AAAGATCTTG TTAATACCGG ATGGCCGGTC CTGATGGCGC TGAGCAACAA GGATTTTGTC GGGGAGACTC TCGGTGTGGA CCTGACCGAA CGCCTGGAAG GGACGCTCGC CGCAACAGCG CTTGCCGCCG CGGATGGAGC AGCCATGTTC CGGGTACACG AGGTGGGGCC CACTCGGCGC GTGCTCGAAA TGGTCGCGTC GATTCAGGGA GTGCGGAAGC CCGCACGAAC AGTGAGGGGA CTGGCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T