MysmA.00937.a
tRNA (guanine-N(1)-)-methyltransferase
| CENTER ID: | MysmA.00937.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00937.a.A1.GE28852 | Full length( MysmA.00937.a ) | 1 | 225 | ||
| MysmA.00937.a.AE1.GE43162 | Full length( MysmA.00937.a ) | 1 | 225 | ||
| MysmA.00937.a.B1.GE43084 | Full length( MysmA.00937.a ) | 1 | 225 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6W15
DEPOSITED: 3/3/2020
DETERMINATION: XRay
CLONE: MysmA.00937.a.AE1.GE43162
PROTEIN: MysmA.00937.a.AE1.PW38670
6W14
DEPOSITED: 3/3/2020
DETERMINATION: XRay
CLONE: MysmA.00937.a.AE1.GE43162
PROTEIN: MysmA.00937.a.AE1.PW38670
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_132581 |
| PATRIC ID: | fig|246196.19.peg.2403 |
| RefSeq: | YP_886778.1 |
| UniProt: | A0QV40 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIDVISIFP AYLDPIRQSL PGKAIDAGIV SVEVHDLRNW THDVHRSVDD SPYGGGPGMV MKAPVWGEAL DEICSEETLL VVPTPAGRLF DQRTAQRWST ERHLVFACGR YEGIDQRVVD DAARRMRVEE VSIGDYVLNG GEAATLVMVE AVVRLLPDVL GNPASHQQDS HSDGLLEGPS YTRPPSWRGL DVPPVLLSGD HAKVAAWRHE QALQRTRERR PDLLD
NT Sequence
GTGCGTATCG ACGTCATCTC GATATTTCCC GCCTACCTCG ATCCGATCCG GCAGTCGTTG CCGGGCAAGG CCATCGACGC CGGCATCGTC TCCGTCGAGG TGCACGACCT GCGCAACTGG ACCCACGACG TGCACCGTTC CGTGGACGAC TCGCCCTACG GCGGTGGTCC CGGCATGGTG ATGAAAGCTC CGGTGTGGGG TGAGGCGCTC GACGAGATCT GCTCGGAGGA AACGCTTCTC GTGGTGCCGA CGCCTGCGGG CCGGCTGTTC GACCAGCGCA CGGCCCAGCG CTGGAGCACC GAAAGACACC TGGTGTTCGC GTGCGGACGC TACGAGGGCA TCGATCAGCG CGTCGTCGAC GACGCCGCGC GGCGCATGCG CGTCGAAGAG GTCTCGATCG GCGACTACGT GCTCAACGGG GGAGAGGCCG CCACGCTGGT GATGGTCGAG GCCGTGGTGC GCCTGCTGCC CGATGTGCTC GGCAATCCCG CTTCGCACCA ACAGGATTCA CACTCCGACG GGCTGCTCGA AGGACCCAGC TACACGCGCC CGCCGAGCTG GCGCGGGCTC GACGTGCCGC CGGTCCTGCT CTCCGGCGAC CACGCGAAGG TCGCGGCGTG GCGCCACGAG CAGGCCCTGC AGCGCACCCG AGAGCGCCGC CCCGACCTGC TCGAC
Details for MysmA.00937.a.A1.GE28852
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRIDVISI FPAYLDPIRQ SLPGKAIDAG IVSVEVHDLR NWTHDVHRSV DDSPYGGGPG MVMKAPVWGE ALDEICSEET LLVVPTPAGR LFDQRTAQRW STERHLVFAC GRYEGIDQRV VDDAARRMRV EEVSIGDYVL NGGEAATLVM VEAVVRLLPD VLGNPASHQQ DSHSDGLLEG PSYTRPPSWR GLDVPPVLLS GDHAKVAAWR HEQALQRTRE RRPDLLD
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc gtatcgacgt catctcgata tttcccgcct acctcgatcc gatccggcag tcgttgccgg gcaaggccat cgacgccggc atcgtctccg tcgaggtgca cgacctgcgc aactggaccc acgacgtgca ccgttccgtg gacgactcgc cctacggcgg tggtcccggc atggtgatga aagctccggt gtggggtgag gcgctcgacg agatctgctc ggaggaaacg cttctcgtgg tgccgacgcc tgcgggccgg ctgttcgacc agcgcacggc ccagcgctgg agcaccgaaa gacacctggt gttcgcgtgc ggacgctacg agggcatcga tcagcgcgtc gtcgacgacg ccgcgcggcg catgcgcgtc gaagaggtct cgatcggcga ctacgtgctc aacgggggag aggccgccac gctggtgatg gtcgaggccg tggtgcgcct gctgcccgat gtgctcggca atcccgcttc gcaccaacag gattcacact ccgacgggct gctcgaagga cccagctaca cgcgcccgcc gagctggcgc gggctcgacg tgccgccggt cctgctctcc ggcgaccacg cgaaggtcgc ggcgtggcgc cacgagcagg ccctgcagcg cacccgagag cgccgccccg acctgctcga ctaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRIDVISI FPAYLDPIRQ SLPGKAIDAG IVSVEVHDLR NWTHDVHRSV DDSPYGGGPG MVMKAPVWGE ALDEICSEET LLVVPTPAGR LFDQRTAQRW STERHLVFAC GRYEGIDQRV VDDAARRMRV EEVSIGDYVL NGGEAATLVM VEAVVRLLPD VLGNPASHQQ DSHSDGLLEG PSYTRPPSWR GLDVPPVLLS GDHAKVAAWR HEQALQRTRE RRPDLLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgtatc gacgtcatct cgatatttcc cgcctacctc gatccgatcc ggcagtcgtt gccgggcaag gccatcgacg ccggcatcgt ctccgtcgag gtgcacgacc tgcgcaactg gacccacgac gtgcaccgtt ccgtggacga ctcgccctac ggcggtggtc ccggcatggt gatgaaagct ccggtgtggg gtgaggcgct cgacgagatc tgctcggagg aaacgcttct cgtggtgccg acgcctgcgg gccggctgtt cgaccagcgc acggcccagc gctggagcac cgaaagacac ctggtgttcg cgtgcggacg ctacgagggc atcgatcagc gcgtcgtcga cgacgccgcg cggcgcatgc gcgtcgaaga ggtctcgatc ggcgactacg tgctcaacgg gggagaggcc gccacgctgg tgatggtcga ggccgtggtg cgcctgctgc ccgatgtgct cggcaatccc gcttcgcacc aacaggattc acactccgac gggctgctcg aaggacccag ctacacgcgc ccgccgagct ggcgcgggct cgacgtgccg ccggtcctgc tctccggcga ccacgcgaag gtcgcggcgt ggcgccacga gcaggccctg cagcgcaccc gagagcgccg ccccgacctg ctcgacaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for MysmA.00937.a.AE1.GE43162
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 2/5/2020 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MVRIDVISIF PAYLDPIRQS LPGKAIDAGI VSVEVHDLRN WTHDVHRSVD DSPYGGGPGM VMKAPVWGEA LDEICSEETL LVVPTPAGRL FDQRTAQRWS TERHLVFACG RYEGIDQRVV DDAARRMRVE EVSIGDYVLN GGEAATLVMV EAVVRLLPDV LGNPASHQQD SHSDGLLEGP SYTRPPSWRG LDVPPVLLSG DHAKVAAWRH EQALQRTRER RPDLLDAGHH HHHH
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MRIDVISIFP AYLDPIRQSL PGKAIDAGIV SVEVHDLRNW THDVHRSVDD SPYGGGPGMV MKAPVWGEAL DEICSEETLL VVPTPAGRLF DQRTAQRWST ERHLVFACGR YEGIDQRVVD DAARRMRVEE VSIGDYVLNG GEAATLVMVE AVVRLLPDVL GNPASHQQDS HSDGLLEGPS YTRPPSWRGL DVPPVLLSGD HAKVAAWRHE QALQRTRERR PDLLDGHHHH HH
Full NT Sequence (Expression Vector + Insert)
tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccgt gcgtatcgac gtcatctcga tatttcccgc ctacctcgat ccgatccggc agtcgttgcc gggcaaggcc atcgacgccg gcatcgtctc cgtcgaggtg cacgacctgc gcaactggac ccacgacgtg caccgttccg tggacgactc gccctacggc ggtggtcccg gcatggtgat gaaagctccg gtgtggggtg aggcgctcga cgagatctgc tcggaggaaa cgcttctcgt ggtgccgacg cctgcgggcc ggctgttcga ccagcgcacg gcccagcgct ggagcaccga aagacacctg gtgttcgcgt gcggacgcta cgagggcatc gatcagcgcg tcgtcgacga cgccgcgcgg cgcatgcgcg tcgaagaggt ctcgatcggc gactacgtgc tcaacggggg agaggccgcc acgctggtga tggtcgaggc cgtggtgcgc ctgctgcccg atgtgctcgg caatcccgct tcgcaccaac aggattcaca ctccgacggg ctgctcgaag gacccagcta cacgcgcccg ccgagctggc gcgggctcga cgtgccgccg gtcctgctct ccggcgacca cgcgaaggtc gcggcgtggc gccacgagca ggccctgcag cgcacccgag agcgccgccc cgacctgctc gacgggcacc accatcatca tcattaacgg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga t
Details for MysmA.00937.a.B1.GE43084
| HARVESTED ON: | 4/19/2019 |
| SEQUENCED ON: | 4/23/2019 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV RIDVISIFPA YLDPIRQSLP GKAIDAGIVS VEVHDLRNWT HDVHRSVDDS PYGGGPGMVM KAPVWGEALD EICSEETLLV VPTPAGRLFD QRTAQRWSTE RHLVFACGRY EGIDQRVVDD AARRMRVEEV SIGDYVLNGG EAATLVMVEA VVRLLPDVLG NPASHQQDSH SDGLLEGPSY TRPPSWRGLD VPPVLLSGDH AKVAAWRHEQ ALQRTRXRRP DLLD
Validated NT Sequence
atcttactca cttagtcgag caggtcgggg cggcgctntc gggtgcgctg cagggcctgc tcgtggcgcc acgccgcgac cttcgcgtgg tcgccggaga gcaggaccgg cggcacgtcg agcccgcgcc agctcggcgg gcgcgtgtag ctgggtcctt cgagcagccc gtcggagtgt gaatcctgtt ggtgcgaagc gggattgccg agcacatcgg gcagcaggcg caccacggcc tcgaccatca ccagcgtggc ggcctctccc ccgttgagca cgtagtcgcc gatcgagacc tcttcgacgc gcatgcgccg cgcggcgtcg tcgacgacgc gctgatcgat gccctcgtag cgtccgcacg cgaacaccag gtgtctttcg gtgctccagc gctgggccgt gcgctggtcg aacagccggc ccgcaggcgt cggcaccacg agaagcgttt cctccgagca gatctcgtcg agcgcctcac cccacaccgg agctttcatc accatgccgg gaccaccgcc gtagggcgag tcgtccacgg aacggtgcac gtcgtgggtc cagttgcgca ggtcgtgcac ctcgacggag acgatgccgg cgtcgatggc cttgcccggc aacgactgcc ggatcggatc gaggtaggcg ggaaatatcg agatgacgtc gatacgcacc atatggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMR IDVISIFPAY LDPIRQSLPG KAIDAGIVSV EVHDLRNWTH DVHRSVDDSP YGGGPGMVMK APVWGEALDE ICSEETLLVV PTPAGRLFDQ RTAQRWSTER HLVFACGRYE GIDQRVVDDA ARRMRVEEVS IGDYVLNGGE AATLVMVEAV VRLLPDVLGN PASHQQDSHS DGLLEGPSYT RPPSWRGLDV PPVLLSGDHA KVAAWRHEQA LQRTRERRPD LLD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgta tcgacgtcat ctcgatattt cccgcctacc tcgatccgat ccggcagtcg ttgccgggca aggccatcga cgccggcatc gtctccgtcg aggtgcacga cctgcgcaac tggacccacg acgtgcaccg ttccgtggac gactcgccct acggcggtgg tcccggcatg gtgatgaaag ctccggtgtg gggtgaggcg ctcgacgaga tctgctcgga ggaaacgctt ctcgtggtgc cgacgcctgc gggccggctg ttcgaccagc gcacggccca gcgctggagc accgaaagac acctggtgtt cgcgtgcgga cgctacgagg gcatcgatca gcgcgtcgtc gacgacgccg cgcggcgcat gcgcgtcgaa gaggtctcga tcggcgacta cgtgctcaac gggggagagg ccgccacgct ggtgatggtc gaggccgtgg tgcgcctgct gcccgatgtg ctcggcaatc ccgcttcgca ccaacaggat tcacactccg acgggctgct cgaaggaccc agctacacgc gcccgccgag ctggcgcggg ctcgacgtgc cgccggtcct gctctccggc gaccacgcga aggtcgcggc gtggcgccac gagcaggccc tgcagcgcac ccgagagcgc cgccccgacc tgctcgactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat