SSGCID: Target Page

MysmA.00896.a

Citrate synthase

CENTER ID:	MysmA.00896.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00896.a.A1.GE28848	Full length( MysmA.00896.a )		1	434

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00896.a.A1.PW28753	Full length( MysmA.00896.a )		1	434

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.5527
RefSeq:	YP_889905.1
UniProt:	A0R417

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEKPSNGQE HATVKYPGGE LDLEIVHATE GADGIALGSL LAKTGYTTYD GGFVNTASTK SAITYIDGDA GILRYRGYPI EQLAEKSTFI EVSYLLIYGE LPTAEQLETF TNQIQRHTLL HEDLKRFFDG FPRNAHPMPV LSSAVNALSA YYQDSLDPLD NKQVELSTIR LLAKLPTIAA YAYKKSEGQP FLYPDNSLSL VENFLRMTFG FPAEPYEVDP EIVRALDMLL ILHADHEQNC STSTVRLVGS SQANLFTSIS GGINALWGPL HGGANQAVLE MLEKIRSQGG DVSDFVRKVK NREDGVKLMG FGHRVYKNYD PRARIVKEQA DKILGKLGGD DELLDIAKQL EEIALTDDFF IERKLYPNVD YYTGVIYRAM GFPTRMFTVL FALGRLPGWI AHWREMHSEP NKIGRPRQIY TGYTERGYTD IAGR

NT Sequence

GTGGCCGAAA AACCGTCGAA TGGGCAGGAG CACGCCACCG TCAAGTACCC GGGTGGCGAG CTGGATCTGG AGATCGTCCA TGCCACCGAG GGCGCTGACG GTATCGCGCT GGGCTCATTG CTGGCCAAGA CCGGCTACAC GACCTACGAC GGCGGGTTCG TCAACACCGC CTCGACCAAG AGCGCGATCA CGTACATCGA CGGTGACGCG GGGATCCTGC GGTATCGCGG CTACCCGATC GAGCAGCTTG CGGAGAAGTC GACGTTCATC GAGGTCAGCT ATCTGCTGAT CTACGGCGAA CTCCCCACCG CCGAGCAGTT GGAGACGTTC ACCAACCAGA TCCAGCGGCA CACGCTGCTG CACGAGGATC TCAAGCGGTT CTTCGACGGT TTCCCGCGCA ACGCGCACCC CATGCCGGTG CTGTCCAGCG CGGTCAACGC GCTCTCGGCG TACTACCAGG ACTCGCTGGA TCCGCTGGAC AACAAGCAGG TCGAGCTGTC GACCATCCGC CTGTTGGCCA AGCTACCGAC GATCGCGGCG TACGCGTACA AGAAGTCCGA GGGCCAGCCG TTCCTGTACC CGGACAACTC GCTGAGCCTG GTGGAGAACT TCCTGCGGAT GACGTTCGGT TTCCCGGCCG AGCCCTACGA GGTCGACCCG GAGATCGTGC GCGCGCTGGA CATGCTGTTG ATCCTGCACG CCGATCACGA GCAGAACTGC TCGACCTCGA CGGTGCGTCT GGTGGGCAGC TCGCAGGCCA ACCTGTTCAC CTCGATCTCC GGTGGCATCA ACGCGCTGTG GGGCCCGCTG CACGGCGGCG CCAACCAGGC CGTGCTGGAG ATGCTGGAGA AGATCCGCTC GCAGGGCGGC GACGTCAGCG ACTTCGTACG CAAGGTCAAA AACCGCGAGG ACGGCGTGAA GCTCATGGGC TTCGGGCACC GCGTGTACAA GAACTACGAT CCGCGGGCGC GCATCGTCAA GGAGCAGGCC GACAAGATCC TGGGCAAGCT CGGCGGCGAC GACGAGCTGC TGGACATCGC CAAGCAGCTC GAGGAGATCG CGCTCACCGA CGACTTCTTC ATCGAGCGCA AGCTCTACCC GAACGTCGAC TACTACACCG GCGTGATCTA CCGGGCCATG GGCTTCCCGA CGCGCATGTT CACGGTGCTG TTCGCGCTGG GCCGGCTGCC GGGCTGGATC GCGCACTGGC GTGAGATGCA CTCCGAGCCC AACAAGATCG GCCGTCCGCG CCAGATCTAC ACCGGCTACA CCGAGCGCGG CTACACCGAC ATCGCCGGCC GC

Details for MysmA.00896.a.A1.GE28848

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	50kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	68
PERCENT COVERAGE:	48

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAEKPSNG QEHATVKYPG GELDLEIVHA TEGADGIALG SLLAKTGYTT YDGGFVNTAS TKSAITYIDG DAGILRYRGY PIEQLAEKST FIEVSYLLIY GELPTAXXXX XSPTRSSGTR CCTRISSGSS TVSRATRTPC RCCPARSTRS RRTTRTRWIR WTTSRSSCRP SACWPSYRRS RRTRTRSPRA SRSCTRTTR

Validated NT Sequence

taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg gtggccgaaa aaccgtcgaa tgggcaggag cacgccaccg tcaagtaccc gggtggcgag ctggatctgg agatcgtcca tgccaccgag ggcgctgacg gtatcgcgct gggctcattg ctggccaaga ccggctacac gacctacgac ggcgggttcg tcaacaccgc ctcgaccaag agcgcgatca cgtacatcga cggtgacgcg gggatcctgc ggtatcgcgg ctacccgatc gagcagcttg cggagaagtc gacgttcatc gaggtcagct atctgctgat ctacggcgaa ctccccaccg cnnnnnnttn nnnangttca ccaaccagat ccagcggcac acgctgctgc acgaggatct caagcggttc ttcgacggtt tcccgcgcaa cgcgcacccc atgccggtgc tgtccagcgc ggtcaacgcg ctctcggcgt actaccagga ctcgctggat ccgctggaca acaagcaggt cgagctgtcg accatccgcc tgttggccaa gctaccgacg atcgcggcgt acgcgtacaa gaagtccgag ggccagccgt tcctgtaccc ggacaactcg ctgagcctgg tggagaactt cctgcggatg acgttcggtt tcccggccga gccctacgag gtcgacccgg agatcgtgcg cgcgctggac atgctgttga tcctgcacgc cgatcacgag cagaactgct cgacctcgac ggtgcgtctg gtgggcagct cgcagnccaa cctgttcacc tcgatctccg gtggcatcaa cgcgctgtgg ggcccgctgc acggcggcgc caaccaggcc gtgctggaga tgctggagaa gatccgctcg cagggcggcg acgtcagcga cttcgtacgc aaggtcaaaa accgcgagga cggcgtgaag ctcatgggct tcgggc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVAEKPSNG QEHATVKYPG GELDLEIVHA TEGADGIALG SLLAKTGYTT YDGGFVNTAS TKSAITYIDG DAGILRYRGY PIEQLAEKST FIEVSYLLIY GELPTAEQLE TFTNQIQRHT LLHEDLKRFF DGFPRNAHPM PVLSSAVNAL SAYYQDSLDP LDNKQVELST IRLLAKLPTI AAYAYKKSEG QPFLYPDNSL SLVENFLRMT FGFPAEPYEV DPEIVRALDM LLILHADHEQ NCSTSTVRLV GSSQANLFTS ISGGINALWG PLHGGANQAV LEMLEKIRSQ GGDVSDFVRK VKNREDGVKL MGFGHRVYKN YDPRARIVKE QADKILGKLG GDDELLDIAK QLEEIALTDD FFIERKLYPN VDYYTGVIYR AMGFPTRMFT VLFALGRLPG WIAHWREMHS EPNKIGRPRQ IYTGYTERGY TDIAGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgaa aaaccgtcga atgggcagga gcacgccacc gtcaagtacc cgggtggcga gctggatctg gagatcgtcc atgccaccga gggcgctgac ggtatcgcgc tgggctcatt gctggccaag accggctaca cgacctacga cggcgggttc gtcaacaccg cctcgaccaa gagcgcgatc acgtacatcg acggtgacgc ggggatcctg cggtatcgcg gctacccgat cgagcagctt gcggagaagt cgacgttcat cgaggtcagc tatctgctga tctacggcga actccccacc gccgagcagt tggagacgtt caccaaccag atccagcggc acacgctgct gcacgaggat ctcaagcggt tcttcgacgg tttcccgcgc aacgcgcacc ccatgccggt gctgtccagc gcggtcaacg cgctctcggc gtactaccag gactcgctgg atccgctgga caacaagcag gtcgagctgt cgaccatccg cctgttggcc aagctaccga cgatcgcggc gtacgcgtac aagaagtccg agggccagcc gttcctgtac ccggacaact cgctgagcct ggtggagaac ttcctgcgga tgacgttcgg tttcccggcc gagccctacg aggtcgaccc ggagatcgtg cgcgcgctgg acatgctgtt gatcctgcac gccgatcacg agcagaactg ctcgacctcg acggtgcgtc tggtgggcag ctcgcaggcc aacctgttca cctcgatctc cggtggcatc aacgcgctgt ggggcccgct gcacggcggc gccaaccagg ccgtgctgga gatgctggag aagatccgct cgcagggcgg cgacgtcagc gacttcgtac gcaaggtcaa aaaccgcgag gacggcgtga agctcatggg cttcgggcac cgcgtgtaca agaactacga tccgcgggcg cgcatcgtca aggagcaggc cgacaagatc ctgggcaagc tcggcggcga cgacgagctg ctggacatcg ccaagcagct cgaggagatc gcgctcaccg acgacttctt catcgagcgc aagctctacc cgaacgtcga ctactacacc ggcgtgatct accgggccat gggcttcccg acgcgcatgt tcacggtgct gttcgcgctg ggccggctgc cgggctggat cgcgcactgg cgtgagatgc actccgagcc caacaagatc ggccgtccgc gccagatcta caccggctac accgagcgcg gctacaccga catcgccggc cgcaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for MysmA.00896.a.A1.PW28753

PURIFICATION DATe:	5/3/2010
CONCENTRATION:	25.65mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	100µl
PERCENT IDENTITY:	68
PERCENT COVERAGE:	48

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGGCCGAA AAACCGTCGA ATGGGCAGGA GCACGCCACC GTCAAGTACC CGGGTGGCGA GCTGGATCTG GAGATCGTCC ATGCCACCGA GGGCGCTGAC GGTATCGCGC TGGGCTCATT GCTGGCCAAG ACCGGCTACA CGACCTACGA CGGCGGGTTC GTCAACACCG CCTCGACCAA GAGCGCGATC ACGTACATCG ACGGTGACGC GGGGATCCTG CGGTATCGCG GCTACCCGAT CGAGCAGCTT GCGGAGAAGT CGACGTTCAT CGAGGTCAGC TATCTGCTGA TCTACGGCGA ACTCCCCACC GCCGAGCAGT TGGAGACGTT CACCAACCAG ATCCAGCGGC ACACGCTGCT GCACGAGGAT CTCAAGCGGT TCTTCGACGG TTTCCCGCGC AACGCGCACC CCATGCCGGT GCTGTCCAGC GCGGTCAACG CGCTCTCGGC GTACTACCAG GACTCGCTGG ATCCGCTGGA CAACAAGCAG GTCGAGCTGT CGACCATCCG CCTGTTGGCC AAGCTACCGA CGATCGCGGC GTACGCGTAC AAGAAGTCCG AGGGCCAGCC GTTCCTGTAC CCGGACAACT CGCTGAGCCT GGTGGAGAAC TTCCTGCGGA TGACGTTCGG TTTCCCGGCC GAGCCCTACG AGGTCGACCC GGAGATCGTG CGCGCGCTGG ACATGCTGTT GATCCTGCAC GCCGATCACG AGCAGAACTG CTCGACCTCG ACGGTGCGTC TGGTGGGCAG CTCGCAGGCC AACCTGTTCA CCTCGATCTC CGGTGGCATC AACGCGCTGT GGGGCCCGCT GCACGGCGGC GCCAACCAGG CCGTGCTGGA GATGCTGGAG AAGATCCGCT CGCAGGGCGG CGACGTCAGC GACTTCGTAC GCAAGGTCAA AAACCGCGAG GACGGCGTGA AGCTCATGGG CTTCGGGCAC CGCGTGTACA AGAACTACGA TCCGCGGGCG CGCATCGTCA AGGAGCAGGC CGACAAGATC CTGGGCAAGC TCGGCGGCGA CGACGAGCTG CTGGACATCG CCAAGCAGCT CGAGGAGATC GCGCTCACCG ACGACTTCTT CATCGAGCGC AAGCTCTACC CGAACGTCGA CTACTACACC GGCGTGATCT ACCGGGCCAT GGGCTTCCCG ACGCGCATGT TCACGGTGCT GTTCGCGCTG GGCCGGCTGC CGGGCTGGAT CGCGCACTGG CGTGAGATGC ACTCCGAGCC CAACAAGATC GGCCGTCCGC GCCAGATCTA CACCGGCTAC ACCGAGCGCG GCTACACCGA CATCGCCGGC CGCAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA