MysmA.00797.a

ABC transporter, ATP-binding protein

CENTER ID: MysmA.00797.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00797.a.A1.GE28846 Full length( MysmA.00797.a ) 1 291
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.1487
RefSeq: YP_885884.1
UniProt: A0QSJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSASEVIDV RDLTFTYPRA AEPAVRGMDF AVGNGEIFGF LGPSGAGKST TQKLLIGLLR GHGGQALVWG RDPVDWGQDY YQRVGVSFEL PNHYLRLTGL ENLQFFASLY EKQTEDPMEL LHAVGLADAA YTRVGEYSKG MQMRLTFARS LINKPELLFL DEPTSGLDPV NARRVKEIVL DLKSKGRTIF LTTHDMSTAD ELCDRIAFVV DGSIVALDTP AELKIARSRR RVRVQYRVAD GGLRTAEFGM DGLADDPEFH AVLRDHHVET IHSREASLDD VFVEVTGRQL A
NT Sequence
ATGGGTTCTG CGAGCGAGGT GATCGACGTT CGAGACCTCA CCTTCACCTA TCCCAGGGCG GCCGAACCCG CCGTCCGCGG AATGGATTTC GCCGTAGGCA ACGGGGAGAT CTTCGGGTTC CTCGGCCCCA GCGGCGCAGG TAAGTCGACG ACGCAGAAAC TGCTGATCGG GCTGCTACGC GGGCACGGCG GCCAGGCATT GGTGTGGGGC CGCGACCCGG TGGACTGGGG CCAGGACTAC TACCAGCGCG TCGGTGTGTC GTTCGAGCTG CCGAACCACT ACCTCAGGCT GACGGGCCTG GAGAACCTGC AGTTCTTCGC CTCCCTCTAC GAAAAGCAGA CCGAGGACCC CATGGAACTA CTGCACGCGG TCGGTCTCGC CGATGCCGCG TACACCCGGG TCGGCGAGTA CTCCAAGGGG ATGCAGATGC GTCTCACCTT TGCGCGGTCT TTGATCAACA AACCCGAACT GCTGTTCCTC GACGAGCCGA CCTCAGGTCT GGACCCGGTC AACGCGCGCA GGGTCAAAGA GATCGTCCTC GATCTGAAAT CGAAGGGCCG CACCATCTTC CTGACCACCC ATGACATGTC GACGGCTGAC GAATTGTGCG ACCGGATCGC GTTCGTCGTC GACGGCAGCA TCGTCGCGCT CGACACCCCG GCAGAGCTCA AGATCGCGCG AAGCCGGCGA CGGGTGCGTG TGCAGTACCG GGTGGCCGAT GGAGGTCTGC GCACGGCCGA ATTCGGGATG GACGGGCTGG CAGACGATCC GGAGTTCCAC GCGGTGCTCC GTGATCACCA CGTCGAGACC ATCCACAGCC GGGAGGCCAG TCTCGACGAC GTGTTCGTCG AGGTCACCGG ACGGCAGCTG GCA
Details for MysmA.00797.a.A1.GE28846
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 34kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 84
Validated AA Sequence
MDFAVGNGEI FGFLGPSGAG KSTTQKLLIG LLRGHGGQAL VWGRDPVDWG QDYYQRVGVS FELPNHYLRL TGLENLQFFA SLYEKQTEDP MELLHAVGLA DAAYTRVGEY SKGMQMRLTF ARSLINKPEL LFLDEPTSGL DPVNARRVKE IVLDLKSKGR TIFLTTHDMS TADELCDRIA FVVDGSIVAL DTPAELKIAR SRRRVRVQYR VADGGLRTAE FGMDGLADDP EFHAVLRDHH VETIHSREAS LDDVFVEVTG RQLA
Validated NT Sequence
cgangggttc tgcgagcgag gtgatcgacg ttcgagacct caccttcacc tatcccaggg cggccgaacc cgccgtccgc ggaatggatt tcgccgtagg caacggggag atcttcgggt tcctcggccc cagcggcgca ggtaagtcga cgacgcagaa actgctgatc gggctgctac gcgggcacgg cggccaggca ttggtgtggg gccgcgaccc ggtggactgg ggccaggact actaccagcg cgtcggtgtg tcgttcgagc tgccgaacca ctacctcagg ctgacgggcc tggagaacct gcagttcttc gcctccctct acgaaaagca gaccgaggac cccatggaac tactgcacgc ggtcggtctc gccgatgccg cgtacacccg ggtcggcgag tactccaagg ggatgcagat gcgtctcacc tttgcgcggt ctttgatcaa caaacccgaa ctgctgttcc tcgacgagcc gacctcaggt ctggacccgg tcaacgcgcg cagggtcaaa gagatcgtcc tcgatctgaa atcgaagggc cgcaccatct tcctgaccac ccatgacatg tcgacggctg acgaattgtg cgaccggatc gcgttcgtcg tcgacggcag catcgtcgcg ctcgacaccc cggcagagct caagatcgcg cgaagccggc gacgggtgcg tgtgcagtac cgggtggccg atggaggtct gcgcacggcc gaattcggga tggacgggct ggcagacgat ccggagttcc acgcggtgct ccgtgatcac cacgtcgaga ccatccacag ccgggaggcc agtctcgacg acgtgttcgt cgaggtcacc ggacggcagc tggcataat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGSASEVID VRDLTFTYPR AAEPAVRGMD FAVGNGEIFG FLGPSGAGKS TTQKLLIGLL RGHGGQALVW GRDPVDWGQD YYQRVGVSFE LPNHYLRLTG LENLQFFASL YEKQTEDPME LLHAVGLADA AYTRVGEYSK GMQMRLTFAR SLINKPELLF LDEPTSGLDP VNARRVKEIV LDLKSKGRTI FLTTHDMSTA DELCDRIAFV VDGSIVALDT PAELKIARSR RRVRVQYRVA DGGLRTAEFG MDGLADDPEF HAVLRDHHVE TIHSREASLD DVFVEVTGRQ LA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggttctgcg agcgaggtga tcgacgttcg agacctcacc ttcacctatc ccagggcggc cgaacccgcc gtccgcggaa tggatttcgc cgtaggcaac ggggagatct tcgggttcct cggccccagc ggcgcaggta agtcgacgac gcagaaactg ctgatcgggc tgctacgcgg gcacggcggc caggcattgg tgtggggccg cgacccggtg gactggggcc aggactacta ccagcgcgtc ggtgtgtcgt tcgagctgcc gaaccactac ctcaggctga cgggcctgga gaacctgcag ttcttcgcct ccctctacga aaagcagacc gaggacccca tggaactact gcacgcggtc ggtctcgccg atgccgcgta cacccgggtc ggcgagtact ccaaggggat gcagatgcgt ctcacctttg cgcggtcttt gatcaacaaa cccgaactgc tgttcctcga cgagccgacc tcaggtctgg acccggtcaa cgcgcgcagg gtcaaagaga tcgtcctcga tctgaaatcg aagggccgca ccatcttcct gaccacccat gacatgtcga cggctgacga attgtgcgac cggatcgcgt tcgtcgtcga cggcagcatc gtcgcgctcg acaccccggc agagctcaag atcgcgcgaa gccggcgacg ggtgcgtgtg cagtaccggg tggccgatgg aggtctgcgc acggccgaat tcgggatgga cgggctggca gacgatccgg agttccacgc ggtgctccgt gatcaccacg tcgagaccat ccacagccgg gaggccagtc tcgacgacgt gttcgtcgag gtcaccggac ggcagctggc aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa