MysmA.00786.a

Nucleoside-triphosphatase

CENTER ID: MysmA.00786.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00786.a.A1.GE28845 Full length( MysmA.00786.a ) 1 200
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00786.a.A1.PW28750 Full length( MysmA.00786.a ) 1 200

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4781
RefSeq: YP_889155.1
UniProt: A0R1W7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTELLVASRN KKKLAELRRV LDAAGISGLR LLSLDDVAPY DESPETGATF EENALAKARD GFEATGLACV ADDSGITVAA LNGMPGVLSA RWSGTHGNDA ANTELLLAQL ADVPDERRGA AFVSACALVS ASGSTVVRGE WPGTVTREPR GDGGFGYDPV FLPEGSERTA AELTPAEKDA SSHRGRALTL LVPALRALEG
NT Sequence
GTGACTGAAC TGCTCGTGGC CAGCCGCAAC AAGAAGAAGC TGGCCGAACT GAGGCGTGTG CTCGACGCGG CGGGCATATC GGGTCTGCGG CTGTTGTCCC TCGACGACGT CGCCCCGTAC GACGAATCAC CCGAGACCGG TGCGACTTTC GAGGAGAACG CGTTGGCCAA GGCCCGCGAC GGGTTCGAGG CGACCGGGCT GGCGTGCGTG GCCGACGACT CGGGGATCAC CGTCGCGGCG CTCAACGGCA TGCCGGGAGT GCTGTCGGCA CGCTGGTCCG GCACGCACGG CAACGACGCC GCCAACACCG AACTGCTGCT CGCGCAACTG GCCGATGTGC CCGACGAGCG CCGCGGCGCG GCGTTCGTGT CGGCGTGCGC GTTGGTGTCG GCCTCGGGAT CGACTGTGGT GCGCGGCGAG TGGCCCGGCA CGGTCACGCG TGAACCCCGC GGCGACGGCG GGTTCGGCTA CGACCCGGTG TTCCTGCCCG AGGGGTCGGA GCGCACGGCC GCCGAGCTCA CCCCGGCCGA GAAGGACGCG TCCTCACACC GCGGCCGCGC GTTGACGCTC CTGGTGCCCG CGCTGCGCGC GTTGGAGGGT
Details for MysmA.00786.a.A1.GE28845
HARVESTED ON: 3/23/2010
SEQUENCED ON: 4/13/2010
EXPECTED MW: 23kDa
OBSERVED MW: 23kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELLVAS RNKKKLAELR RVLDAAGISG LRLLSLDDVA PYDESPETGA TFEENALAKA RDGFEATGLA CVADDSGITV AALNGMPGVL SARWSGTHGN DAANTELLLA QLADVPDERR GAAFVSACAL VSASGSTVVR GEWPGTVTRE PRGDGGFGYD PVFLPEGSER TAAELTPAEK DASSHRGRAL TLLVPALRAL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ctgaactgct cgtggccagc cgcaacaaga agaagctggc cgaactgagg cgtgtgctcg acgcggcggg catatcgggt ctgcggctgt tgtccctcga cgacgtcgcc ccgtacgacg aatcacccga gaccggtgcg actttcgagg agaacgcgtt ggccaaggcc cgcgacgggt tcgaggcgac cgggctggcg tgcgtggccg acgactcggg gatcaccgtc gcggcgctca acggcatgcc gggagtgctg tcggcacgct ggtccggcac gcacggcaac gacgccgcca acaccgaact gctgctcgcg caactggccg atgtgcccga cgagcgccgc ggcgcggcgt tcgtgtcggc gtgcgcgttg gtgtcggcct cgggatcgac tgtggtgcgc ggcgagtggc ccggcacggt cacgcgtgaa ccccgcggcg acggcgggtt cggctacgac ccggtgttcc tgcccgaggg gtcggagcgc acggccgccg agctcacccc ggccgagaag gacgcgtcct cacaccgcgg ccgcgcgttg acgctcctgg tgcccgcgct gcgcgcgttg g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELLVAS RNKKKLAELR RVLDAAGISG LRLLSLDDVA PYDESPETGA TFEENALAKA RDGFEATGLA CVADDSGITV AALNGMPGVL SARWSGTHGN DAANTELLLA QLADVPDERR GAAFVSACAL VSASGSTVVR GEWPGTVTRE PRGDGGFGYD PVFLPEGSER TAAELTPAEK DASSHRGRAL TLLVPALRAL EG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgaa ctgctcgtgg ccagccgcaa caagaagaag ctggccgaac tgaggcgtgt gctcgacgcg gcgggcatat cgggtctgcg gctgttgtcc ctcgacgacg tcgccccgta cgacgaatca cccgagaccg gtgcgacttt cgaggagaac gcgttggcca aggcccgcga cgggttcgag gcgaccgggc tggcgtgcgt ggccgacgac tcggggatca ccgtcgcggc gctcaacggc atgccgggag tgctgtcggc acgctggtcc ggcacgcacg gcaacgacgc cgccaacacc gaactgctgc tcgcgcaact ggccgatgtg cccgacgagc gccgcggcgc ggcgttcgtg tcggcgtgcg cgttggtgtc ggcctcggga tcgactgtgg tgcgcggcga gtggcccggc acggtcacgc gtgaaccccg cggcgacggc gggttcggct acgacccggt gttcctgccc gaggggtcgg agcgcacggc cgccgagctc accccggccg agaaggacgc gtcctcacac cgcggccgcg cgttgacgct cctggtgccc gcgctgcgcg cgttggaggg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa
Details for MysmA.00786.a.A1.PW28750
PURIFICATION DATe: 5/3/2010
CONCENTRATION: 24.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELLVAS RNKKKLAELR RVLDAAGISG LRLLSLDDVA PYDESPETGA TFEENALAKA RDGFEATGLA CVADDSGITV AALNGMPGVL SARWSGTHGN DAANTELLLA QLADVPDERR GAAFVSACAL VSASGSTVVR GEWPGTVTRE PRGDGGFGYD PVFLPEGSER TAAELTPAEK DASSHRGRAL TLLVPALRAL
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ctgaactgct cgtggccagc cgcaacaaga agaagctggc cgaactgagg cgtgtgctcg acgcggcggg catatcgggt ctgcggctgt tgtccctcga cgacgtcgcc ccgtacgacg aatcacccga gaccggtgcg actttcgagg agaacgcgtt ggccaaggcc cgcgacgggt tcgaggcgac cgggctggcg tgcgtggccg acgactcggg gatcaccgtc gcggcgctca acggcatgcc gggagtgctg tcggcacgct ggtccggcac gcacggcaac gacgccgcca acaccgaact gctgctcgcg caactggccg atgtgcccga cgagcgccgc ggcgcggcgt tcgtgtcggc gtgcgcgttg gtgtcggcct cgggatcgac tgtggtgcgc ggcgagtggc ccggcacggt cacgcgtgaa ccccgcggcg acggcgggtt cggctacgac ccggtgttcc tgcccgaggg gtcggagcgc acggccgccg agctcacccc ggccgagaag gacgcgtcct cacaccgcgg ccgcgcgttg acgctcctgg tgcccgcgct gcgcgcgttg g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTELLVAS RNKKKLAELR RVLDAAGISG LRLLSLDDVA PYDESPETGA TFEENALAKA RDGFEATGLA CVADDSGITV AALNGMPGVL SARWSGTHGN DAANTELLLA QLADVPDERR GAAFVSACAL VSASGSTVVR GEWPGTVTRE PRGDGGFGYD PVFLPEGSER TAAELTPAEK DASSHRGRAL TLLVPALRAL EG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTGAA CTGCTCGTGG CCAGCCGCAA CAAGAAGAAG CTGGCCGAAC TGAGGCGTGT GCTCGACGCG GCGGGCATAT CGGGTCTGCG GCTGTTGTCC CTCGACGACG TCGCCCCGTA CGACGAATCA CCCGAGACCG GTGCGACTTT CGAGGAGAAC GCGTTGGCCA AGGCCCGCGA CGGGTTCGAG GCGACCGGGC TGGCGTGCGT GGCCGACGAC TCGGGGATCA CCGTCGCGGC GCTCAACGGC ATGCCGGGAG TGCTGTCGGC ACGCTGGTCC GGCACGCACG GCAACGACGC CGCCAACACC GAACTGCTGC TCGCGCAACT GGCCGATGTG CCCGACGAGC GCCGCGGCGC GGCGTTCGTG TCGGCGTGCG CGTTGGTGTC GGCCTCGGGA TCGACTGTGG TGCGCGGCGA GTGGCCCGGC ACGGTCACGC GTGAACCCCG CGGCGACGGC GGGTTCGGCT ACGACCCGGT GTTCCTGCCC GAGGGGTCGG AGCGCACGGC CGCCGAGCTC ACCCCGGCCG AGAAGGACGC GTCCTCACAC CGCGGCCGCG CGTTGACGCT CCTGGTGCCC GCGCTGCGCG CGTTGGAGGG TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA