MysmA.00762.b
Short chain dehydrogenase
| CENTER ID: | MysmA.00762.b |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00762.b.A1.GE28838 | Full length( MysmA.00762.b ) | 1 | 262 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00762.b.A1.PS00603 | Full length( MysmA.00762.b ) | 1 | 262 |
Structures
3O38
DEPOSITED: 7/23/2010
DETERMINATION: XRay
CLONE: MysmA.00762.b.A1.GE28838
PROTEIN: MysmA.00762.b.A1.PS00603
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_168406 |
| PATRIC ID: | fig|246196.19.peg.5847 |
| RefSeq: | YP_890236.1 |
| UniProt: | A0R4Z8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLSEAPKEI DGHGLLKGKV VLVTAAAGTG IGSTTARRAL LEGADVVISD YHERRLGETR DQLADLGLGR VEAVVCDVTS TEAVDALITQ TVEKAGRLDV LVNNAGLGGQ TPVVDMTDEE WDRVLNVTLT SVMRATRAAL RYFRGVDHGG VIVNNASVLG WRAQHSQSHY AAAKAGVMAL TRCSAIEAVE FGVRINAVSP SIARHKFLEK TSSSELLDRL ASDEAFGRAA EPWEVAATIA FLASDYSSYM TGEVVSVSSQ RA
NT Sequence
ATGAATCTGT CGGAGGCCCC GAAAGAGATT GACGGGCACG GTCTTCTGAA GGGCAAGGTG GTCCTGGTGA CCGCCGCCGC CGGTACCGGC ATCGGCTCGA CCACCGCGCG GCGTGCGCTG CTCGAGGGTG CCGACGTGGT GATCTCCGAC TACCACGAGC GCAGGCTGGG GGAGACCCGC GATCAGCTGG CCGACCTGGG CCTGGGACGT GTCGAAGCCG TCGTGTGTGA CGTGACCTCC ACCGAGGCCG TCGATGCGCT GATCACCCAG ACCGTCGAGA AGGCCGGACG GCTGGATGTG CTGGTCAACA ACGCCGGCCT GGGCGGGCAG ACACCCGTCG TCGACATGAC AGACGAGGAG TGGGACCGCG TCCTCAACGT CACGCTCACC TCGGTCATGC GCGCCACGCG GGCCGCGTTG CGGTACTTCC GCGGCGTCGA CCACGGCGGT GTCATCGTCA ACAACGCGAG CGTGCTGGGT TGGCGCGCAC AGCATTCGCA GTCGCACTAC GCGGCCGCGA AAGCCGGTGT CATGGCCCTG ACGCGCTGCA GCGCGATCGA GGCCGTCGAG TTCGGCGTGC GCATCAACGC CGTGTCGCCG AGCATCGCGC GGCACAAGTT CCTGGAGAAG ACGTCGTCCT CGGAACTGCT GGACCGCCTG GCCAGTGACG AGGCGTTCGG CCGCGCGGCC GAACCGTGGG AAGTCGCGGC GACCATCGCC TTCCTCGCGA GTGACTACTC CAGTTACATG ACCGGAGAGG TCGTCTCGGT GTCCAGCCAG CGGGCC
Details for MysmA.00762.b.A1.GE28838
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLSEAPKE IDGHGLLKGK VVLVTAAAGT GIGSTTARRA LLEGADVVIS DYHERRLGET RDQLADLGLG RVEAVVCDVT STEAVDALIT QTVEKAGRLD VLVNNAGLGG QTPVVDMTDE EWDRVLNVTL TSVMRATRAA LRYFRGVDHG GVIVNNASVL GWRAQHSQSH YAAAKAGVMA LTRCSAIEAV EFGVRINAVS PSIARHKFLE KTSSSELLDR LASDEAFGRA AEPWEVAATI AFLASDYSSY MTGEVVSVSS QRA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatc tgtcggaggc cccgaaagag attgacgggc acggtcttct gaagggcaag gtggtcctgg tgaccgccgc cgccggtacc ggcatcggct cgaccaccgc gcggcgtgcg ctgctcgagg gtgccgacgt ggtgatctcc gactaccacg agcgcaggct gggggagacc cgcgatcagc tggccgacct gggcctggga cgtgtcgaag ccgtcgtgtg tgacgtgacc tccaccgagg ccgtcgatgc gctgatcacc cagaccgtcg agaaggccgg acggctggat gtgctggtca acaacgccgg cctgggcggg cagacacccg tcgtcgacat gacagacgag gagtgggacc gcgtcctcaa cgtcacgctc acctcggtca tgcgcgccac gcgggccgcg ttgcggtact tccgcggcgt cgaccacggc ggtgtcatcg tcaacaacgc gagcgtgctg ggttggcgcg cacagcattc gcagtcgcac tacgcggccg cgaaagccgg tgtcatggcc ctgacgcgct gcagcgcgat cgaggccgtc gagttcggcg tgcgcatcaa cgccgtgtcg ccgagcatcg cgcggcacaa gttcctggag aagacgtcgt cctcggaact gctggaccgc ctggccagtg acgaggcgtt cggccgcgcg gccgaaccgt gggaagtcgc ggcgaccatc gccttcctcg cgagtgacta ctccagttac atgaccggag aggtcgtctc ggtgtccagc cagcgggcct aataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLSEAPKE IDGHGLLKGK VVLVTAAAGT GIGSTTARRA LLEGADVVIS DYHERRLGET RDQLADLGLG RVEAVVCDVT STEAVDALIT QTVEKAGRLD VLVNNAGLGG QTPVVDMTDE EWDRVLNVTL TSVMRATRAA LRYFRGVDHG GVIVNNASVL GWRAQHSQSH YAAAKAGVMA LTRCSAIEAV EFGVRINAVS PSIARHKFLE KTSSSELLDR LASDEAFGRA AEPWEVAATI AFLASDYSSY MTGEVVSVSS QRA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatctgtcg gaggccccga aagagattga cgggcacggt cttctgaagg gcaaggtggt cctggtgacc gccgccgccg gtaccggcat cggctcgacc accgcgcggc gtgcgctgct cgagggtgcc gacgtggtga tctccgacta ccacgagcgc aggctggggg agacccgcga tcagctggcc gacctgggcc tgggacgtgt cgaagccgtc gtgtgtgacg tgacctccac cgaggccgtc gatgcgctga tcacccagac cgtcgagaag gccggacggc tggatgtgct ggtcaacaac gccggcctgg gcgggcagac acccgtcgtc gacatgacag acgaggagtg ggaccgcgtc ctcaacgtca cgctcacctc ggtcatgcgc gccacgcggg ccgcgttgcg gtacttccgc ggcgtcgacc acggcggtgt catcgtcaac aacgcgagcg tgctgggttg gcgcgcacag cattcgcagt cgcactacgc ggccgcgaaa gccggtgtca tggccctgac gcgctgcagc gcgatcgagg ccgtcgagtt cggcgtgcgc atcaacgccg tgtcgccgag catcgcgcgg cacaagttcc tggagaagac gtcgtcctcg gaactgctgg accgcctggc cagtgacgag gcgttcggcc gcgcggccga accgtgggaa gtcgcggcga ccatcgcctt cctcgcgagt gactactcca gttacatgac cggagaggtc gtctcggtgt ccagccagcg ggccaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for MysmA.00762.b.A1.PS00603
| PURIFICATION DATe: | 5/15/2010 |
| CONCENTRATION: | 53.36mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNLSEAPKE IDGHGLLKGK VVLVTAAAGT GIGSTTARRA LLEGADVVIS DYHERRLGET RDQLADLGLG RVEAVVCDVT STEAVDALIT QTVEKAGRLD VLVNNAGLGG QTPVVDMTDE EWDRVLNVTL TSVMRATRAA LRYFRGVDHG GVIVNNASVL GWRAQHSQSH YAAAKAGVMA LTRCSAIEAV EFGVRINAVS PSIARHKFLE KTSSSELLDR LASDEAFGRA AEPWEVAATI AFLASDYSSY MTGEVVSVSS QRA
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgaatc tgtcggaggc cccgaaagag attgacgggc acggtcttct gaagggcaag gtggtcctgg tgaccgccgc cgccggtacc ggcatcggct cgaccaccgc gcggcgtgcg ctgctcgagg gtgccgacgt ggtgatctcc gactaccacg agcgcaggct gggggagacc cgcgatcagc tggccgacct gggcctggga cgtgtcgaag ccgtcgtgtg tgacgtgacc tccaccgagg ccgtcgatgc gctgatcacc cagaccgtcg agaaggccgg acggctggat gtgctggtca acaacgccgg cctgggcggg cagacacccg tcgtcgacat gacagacgag gagtgggacc gcgtcctcaa cgtcacgctc acctcggtca tgcgcgccac gcgggccgcg ttgcggtact tccgcggcgt cgaccacggc ggtgtcatcg tcaacaacgc gagcgtgctg ggttggcgcg cacagcattc gcagtcgcac tacgcggccg cgaaagccgg tgtcatggcc ctgacgcgct gcagcgcgat cgaggccgtc gagttcggcg tgcgcatcaa cgccgtgtcg ccgagcatcg cgcggcacaa gttcctggag aagacgtcgt cctcggaact gctggaccgc ctggccagtg acgaggcgtt cggccgcgcg gccgaaccgt gggaagtcgc ggcgaccatc gccttcctcg cgagtgacta ctccagttac atgaccggag aggtcgtctc ggtgtccagc cagcgggcct aataa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNLSEAPKE IDGHGLLKGK VVLVTAAAGT GIGSTTARRA LLEGADVVIS DYHERRLGET RDQLADLGLG RVEAVVCDVT STEAVDALIT QTVEKAGRLD VLVNNAGLGG QTPVVDMTDE EWDRVLNVTL TSVMRATRAA LRYFRGVDHG GVIVNNASVL GWRAQHSQSH YAAAKAGVMA LTRCSAIEAV EFGVRINAVS PSIARHKFLE KTSSSELLDR LASDEAFGRA AEPWEVAATI AFLASDYSSY MTGEVVSVSS QRA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAATCTGTCG GAGGCCCCGA AAGAGATTGA CGGGCACGGT CTTCTGAAGG GCAAGGTGGT CCTGGTGACC GCCGCCGCCG GTACCGGCAT CGGCTCGACC ACCGCGCGGC GTGCGCTGCT CGAGGGTGCC GACGTGGTGA TCTCCGACTA CCACGAGCGC AGGCTGGGGG AGACCCGCGA TCAGCTGGCC GACCTGGGCC TGGGACGTGT CGAAGCCGTC GTGTGTGACG TGACCTCCAC CGAGGCCGTC GATGCGCTGA TCACCCAGAC CGTCGAGAAG GCCGGACGGC TGGATGTGCT GGTCAACAAC GCCGGCCTGG GCGGGCAGAC ACCCGTCGTC GACATGACAG ACGAGGAGTG GGACCGCGTC CTCAACGTCA CGCTCACCTC GGTCATGCGC GCCACGCGGG CCGCGTTGCG GTACTTCCGC GGCGTCGACC ACGGCGGTGT CATCGTCAAC AACGCGAGCG TGCTGGGTTG GCGCGCACAG CATTCGCAGT CGCACTACGC GGCCGCGAAA GCCGGTGTCA TGGCCCTGAC GCGCTGCAGC GCGATCGAGG CCGTCGAGTT CGGCGTGCGC ATCAACGCCG TGTCGCCGAG CATCGCGCGG CACAAGTTCC TGGAGAAGAC GTCGTCCTCG GAACTGCTGG ACCGCCTGGC CAGTGACGAG GCGTTCGGCC GCGCGGCCGA ACCGTGGGAA GTCGCGGCGA CCATCGCCTT CCTCGCGAGT GACTACTCCA GTTACATGAC CGGAGAGGTC GTCTCGGTGT CCAGCCAGCG GGCCAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA