MysmA.00762.a
Short chain dehydrogenase
| CENTER ID: | MysmA.00762.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00762.a.A1.GE28837 | Full length( MysmA.00762.a ) | 1 | 260 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3UCX
DEPOSITED: 10/27/2011
DETERMINATION: XRay
CLONE: MysmA.00762.a.A1.GE28837
PROTEIN: MysmA.00762.a.A1.PS00612
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|246196.19.peg.5771 |
| RefSeq: | YP_890157.1 |
| UniProt: | A0R4R9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGGLLTDKVV VISGVGPALG TTLARRCAEQ GADLVLAART VERLEDVAKQ VTDTGRRALS VGTDITDDAQ VAHLVDETMK AYGRVDVVIN NAFRVPSMKP FANTTFEHMR DAIELTVFGA LRLIQGFTPA LEESKGAVVN VNSMVVRHSQ AKYGAYKMAK SALLAMSQTL ATELGEKGIR VNSVLPGYIW GGTLKSYFEH QAGKYGTSVE DIYNAAAAGS DLKRLPTEDE VASAILFMAS DLASGITGQA LDVNCGEYKA
NT Sequence
ATGGGTGGAC TCCTGACCGA CAAAGTTGTG GTCATCAGCG GCGTCGGCCC CGCGCTGGGC ACCACGCTCG CGCGGCGGTG CGCCGAGCAG GGCGCCGATC TGGTGCTCGC GGCCCGCACC GTCGAACGCC TCGAAGACGT CGCCAAGCAG GTCACCGACA CCGGCAGGCG CGCACTGTCG GTCGGCACCG ACATCACCGA CGACGCGCAG GTGGCCCACC TCGTCGACGA GACCATGAAG GCATACGGCC GCGTGGACGT GGTGATCAAC AACGCCTTCC GGGTGCCCTC GATGAAACCG TTCGCCAACA CGACCTTCGA GCACATGCGC GACGCCATCG AGCTCACGGT GTTCGGCGCG CTGCGGCTCA TCCAGGGCTT CACGCCGGCG CTGGAAGAGT CCAAAGGTGC TGTGGTGAAC GTGAATTCGA TGGTGGTGCG GCACTCGCAG GCAAAGTACG GCGCCTACAA GATGGCGAAG TCTGCGTTGC TGGCGATGTC GCAGACGCTG GCCACCGAAC TCGGTGAGAA GGGCATCCGG GTGAATTCTG TGCTGCCCGG CTACATCTGG GGCGGAACAC TCAAGAGCTA CTTCGAACAT CAGGCCGGCA AGTACGGGAC CAGCGTCGAG GACATCTACA ACGCGGCCGC CGCGGGATCC GACCTCAAGC GGCTGCCGAC CGAGGACGAG GTGGCCTCGG CGATCCTGTT CATGGCGTCC GATCTGGCCA GCGGCATCAC CGGGCAGGCG CTGGATGTGA ACTGTGGGGA GTACAAGGCA
Details for MysmA.00762.a.A1.GE28837
| HARVESTED ON: | 3/23/2010 |
| SEQUENCED ON: | 4/13/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGGLLTDKV VVISGVGPAL GTTLARRCAE QGADLVLAAR TVERLEDVAK QVTDTGRRAL SVGTDITDDA QVAHLVDETM KAYGRVDVVI NNAFRVPSMK PFANTTFEHM RDAIELTVFG ALRLIQGFTP ALEESKGAVV NVNSMVVRHS QAKYGAYKMA KSALLAMSQT LATELGEKGI RVNSVLPGYI WGGTLKSYFE HQAGKYGTSV EDIYNAAAAG SDLKRLPTED EVASAILFMA SDLASGITGQ ALDVNCGEY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgggtg gactcctgac cgacaaagtt gtggtcatca gcggcgtcgg ccccgcgctg ggcaccacgc tcgcgcggcg gtgcgccgag cagggcgccg atctggtgct cgcggcccgc accgtcgaac gcctcgaaga cgtcgccaag caggtcaccg acaccggcag gcgcgcactg tcggtcggca ccgacatcac cgacgacgcg caggtggccc acctcgtcga cgagaccatg aaggcatacg gccgcgtgga cgtggtgatc aacaacgcct tccgggtgcc ctcgatgaaa ccgttcgcca acacgacctt cgagcacatg cgcgacgcca tcgagctcac ggtgttcggc gcgctgcggc tcatccaggg cttcacgccg gcgctggaag agtccaaagg tgctgtggtg aacgtgaatt cgatggtggt gcggcactcg caggcaaagt acggcgccta caagatggcg aagtctgcgt tgctggcgat gtcgcagacg ctggccaccg aactcggtga gaagggcatc cgggtgaatt ctgtgctgcc cggctacatc tggggcggaa cactcaagag ctacttcgaa catcaggccg gcaagtacgg gaccagcgtc gaggacatct acaacgcggc cgccgcggga tccgacctca agcggctgcc gaccgaggac gaggtggcct cggcgatcct gttcatggcg tccgatctgg ccagcggcat caccgggcag gcgctggatg tgaactgtgg ggagtaca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGGLLTDKV VVISGVGPAL GTTLARRCAE QGADLVLAAR TVERLEDVAK QVTDTGRRAL SVGTDITDDA QVAHLVDETM KAYGRVDVVI NNAFRVPSMK PFANTTFEHM RDAIELTVFG ALRLIQGFTP ALEESKGAVV NVNSMVVRHS QAKYGAYKMA KSALLAMSQT LATELGEKGI RVNSVLPGYI WGGTLKSYFE HQAGKYGTSV EDIYNAAAAG SDLKRLPTED EVASAILFMA SDLASGITGQ ALDVNCGEYK A
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtggactc ctgaccgaca aagttgtggt catcagcggc gtcggccccg cgctgggcac cacgctcgcg cggcggtgcg ccgagcaggg cgccgatctg gtgctcgcgg cccgcaccgt cgaacgcctc gaagacgtcg ccaagcaggt caccgacacc ggcaggcgcg cactgtcggt cggcaccgac atcaccgacg acgcgcaggt ggcccacctc gtcgacgaga ccatgaaggc atacggccgc gtggacgtgg tgatcaacaa cgccttccgg gtgccctcga tgaaaccgtt cgccaacacg accttcgagc acatgcgcga cgccatcgag ctcacggtgt tcggcgcgct gcggctcatc cagggcttca cgccggcgct ggaagagtcc aaaggtgctg tggtgaacgt gaattcgatg gtggtgcggc actcgcaggc aaagtacggc gcctacaaga tggcgaagtc tgcgttgctg gcgatgtcgc agacgctggc caccgaactc ggtgagaagg gcatccgggt gaattctgtg ctgcccggct acatctgggg cggaacactc aagagctact tcgaacatca ggccggcaag tacgggacca gcgtcgagga catctacaac gcggccgccg cgggatccga cctcaagcgg ctgccgaccg aggacgaggt ggcctcggcg atcctgttca tggcgtccga tctggccagc ggcatcaccg ggcaggcgct ggatgtgaac tgtggggagt acaaggcaaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa