SSGCID: Target Page

MysmA.00746.a

ABC transporter, ATP-binding protein SugC

CENTER ID:	MysmA.00746.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00746.a.A1.GE28832	Full length( MysmA.00746.a )		1	406

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.4936
RefSeq:	YP_889308.1
UniProt:	A0R2C0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAEIVLDRVT KSYPDGAGGV RAAVKEFSMT IADGEFIILV GPSGCGKSTT LNMIAGLEEI TSGELRIGGE RVNEKAPKDR DIAMVFQSYA LYPHMTVRQN IAFPLTLAKV PKAEIAAKVE ETAKILDLSE LLDRKPGQLS GGQRQRVAMG RAIVRSPKAF LMDEPLSNLD AKLRVQMRAE ISRLQDRLGT TTVYVTHDQT EAMTLGDRVV VMLAGEVQQI GTPDELYSSP ANLFVAGFIG SPAMNFFPAT RTDVGVRLPF GEVTLTPHML DLLDKQARPE NIIVGIRPEH IEDSALLDGY ARIRALTFSV RADIVESLGA DKYVHFTTEG AGAESAQLAE LAADSGAGTN QFIARVSADS RVRTGEQIEL AIDTTKLSIF DAATGLNLTR DITPTDPTEA AGPDAG

NT Sequence

ATGGCCGAAA TTGTGTTGGA TCGGGTGACC AAGAGTTACC CCGACGGCGC AGGCGGTGTC AGGGCGGCCG TCAAAGAGTT CTCGATGACG ATCGCCGACG GCGAGTTCAT CATCCTGGTC GGACCGTCCG GATGCGGAAA GTCGACCACG CTCAACATGA TTGCCGGTCT GGAGGAGATC ACCTCCGGTG AGCTGCGCAT CGGTGGGGAA CGCGTCAACG AGAAGGCGCC CAAGGACCGT GACATCGCGA TGGTGTTCCA GTCCTACGCG CTCTACCCGC ACATGACGGT GCGGCAGAAC ATCGCATTCC CGCTCACGCT GGCCAAGGTG CCCAAGGCCG AGATCGCCGC CAAGGTCGAG GAGACCGCGA AGATCCTCGA TCTGAGCGAA CTGCTCGACC GCAAGCCCGG TCAGCTCTCG GGCGGCCAGC GTCAGCGTGT GGCGATGGGA CGCGCGATCG TGCGCAGCCC CAAGGCGTTT CTCATGGACG AGCCGCTGAG CAACCTGGAC GCCAAGCTGC GCGTGCAGAT GCGCGCGGAG ATCTCGCGGC TGCAGGATCG CCTGGGCACC ACCACGGTCT ACGTCACGCA CGACCAGACC GAGGCGATGA CGCTGGGCGA CCGCGTGGTG GTGATGCTCG CCGGCGAGGT GCAGCAGATC GGCACACCCG ACGAGCTCTA CAGCAGCCCC GCCAACCTGT TCGTGGCCGG TTTCATCGGT TCGCCGGCGA TGAACTTCTT CCCGGCGACG CGTACCGATG TCGGTGTGCG CCTGCCGTTC GGCGAGGTGA CACTGACCCC GCACATGCTC GACCTGCTGG ACAAGCAGGC CAGGCCCGAG AACATCATCG TCGGAATCCG GCCCGAGCAC ATCGAGGACT CGGCGCTGCT CGACGGCTAC GCGCGGATCC GCGCGCTGAC GTTCTCGGTG CGTGCCGACA TCGTCGAATC GCTCGGCGCC GACAAGTACG TGCACTTCAC CACCGAGGGT GCCGGCGCCG AGTCGGCCCA ATTGGCCGAA CTCGCCGCGG ATTCGGGTGC GGGCACCAAC CAGTTCATCG CCCGGGTGTC CGCGGACTCG CGGGTGCGCA CGGGTGAGCA GATCGAACTG GCCATCGACA CCACCAAGCT GTCGATCTTC GACGCCGCAA CGGGTCTGAA CCTGACCCGT GACATCACGC CGACGGATCC CACCGAAGCG GCGGGGCCGG ACGCCGGG

Details for MysmA.00746.a.A1.GE28832

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	69

Validated AA Sequence

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggccg aaattgtgtt ggatcgggtg accaagagtt accccgacgg cgcaggcggt gtcagggcgg ccgtcaaaga gttctcgatg acgatcgccg acggcgagtt catcatcctg gtcggaccgt ccggatgcgg aaagtcgacc acgctcaaca tgattgccgg tctggaggag atcacctccg gtgagctgcg catcggtggg gaacgcgtca acgagaaggc gcccaaggac cgtgacatcg cgatggtgtt ccagtcctac gcgctctacc cgcacatgac ggtgcggcag aacatcgcat tcccgctcac gctggccaag gtgcccaagg ccgagatcgc cgccaaggtc gaggagaccg cgaagatcct cgatctgagc gaactgctcg accgcaagcc cggtcagctc tcgggcggcc agcgtcagcg tgtggcgatg ggacgcgcga tcgtgcgcag ccccaaggcg tttctcatgg acgagccgct gagcaacctg gacgccaagc tgcgcgtgca gatgcgcgcg gagatctcgc ggctgcagga tcgcctgggc accaccacgg tctacgtcac gcacgaccag accgaggcga tgacgctggg cgaccgcgtg gtggtgatgc tcgccggcga ggtgcagcag atcggcacac ccgacgagct ctacagcagc cccgccaacc tgttcgtggc cggtttcatc ggttcgccgg cgatgaactt cttcccggcg acgcgtaccg atgtcngtgt gcgnctgccg ttcggngagg tgacactgac cccgcacatg ctcgacctgc tggacaagca ng

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAEIVLDRV TKSYPDGAGG VRAAVKEFSM TIADGEFIIL VGPSGCGKST TLNMIAGLEE ITSGELRIGG ERVNEKAPKD RDIAMVFQSY ALYPHMTVRQ NIAFPLTLAK VPKAEIAAKV EETAKILDLS ELLDRKPGQL SGGQRQRVAM GRAIVRSPKA FLMDEPLSNL DAKLRVQMRA EISRLQDRLG TTTVYVTHDQ TEAMTLGDRV VVMLAGEVQQ IGTPDELYSS PANLFVAGFI GSPAMNFFPA TRTDVGVRLP FGEVTLTPHM LDLLDKQARP ENIIVGIRPE HIEDSALLDG YARIRALTFS VRADIVESLG ADKYVHFTTE GAGAESAQLA ELAADSGAGT NQFIARVSAD SRVRTGEQIE LAIDTTKLSI FDAATGLNLT RDITPTDPTE AAGPDAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgaaatt gtgttggatc gggtgaccaa gagttacccc gacggcgcag gcggtgtcag ggcggccgtc aaagagttct cgatgacgat cgccgacggc gagttcatca tcctggtcgg accgtccgga tgcggaaagt cgaccacgct caacatgatt gccggtctgg aggagatcac ctccggtgag ctgcgcatcg gtggggaacg cgtcaacgag aaggcgccca aggaccgtga catcgcgatg gtgttccagt cctacgcgct ctacccgcac atgacggtgc ggcagaacat cgcattcccg ctcacgctgg ccaaggtgcc caaggccgag atcgccgcca aggtcgagga gaccgcgaag atcctcgatc tgagcgaact gctcgaccgc aagcccggtc agctctcggg cggccagcgt cagcgtgtgg cgatgggacg cgcgatcgtg cgcagcccca aggcgtttct catggacgag ccgctgagca acctggacgc caagctgcgc gtgcagatgc gcgcggagat ctcgcggctg caggatcgcc tgggcaccac cacggtctac gtcacgcacg accagaccga ggcgatgacg ctgggcgacc gcgtggtggt gatgctcgcc ggcgaggtgc agcagatcgg cacacccgac gagctctaca gcagccccgc caacctgttc gtggccggtt tcatcggttc gccggcgatg aacttcttcc cggcgacgcg taccgatgtc ggtgtgcgcc tgccgttcgg cgaggtgaca ctgaccccgc acatgctcga cctgctggac aagcaggcca ggcccgagaa catcatcgtc ggaatccggc ccgagcacat cgaggactcg gcgctgctcg acggctacgc gcggatccgc gcgctgacgt tctcggtgcg tgccgacatc gtcgaatcgc tcggcgccga caagtacgtg cacttcacca ccgagggtgc cggcgccgag tcggcccaat tggccgaact cgccgcggat tcgggtgcgg gcaccaacca gttcatcgcc cgggtgtccg cggactcgcg ggtgcgcacg ggtgagcaga tcgaactggc catcgacacc accaagctgt cgatcttcga cgccgcaacg ggtctgaacc tgacccgtga catcacgccg acggatccca ccgaagcggc ggggccggac gccgggaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa