MysmA.00698.a

Citrate synthase 2

CENTER ID: MysmA.00698.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00698.a.A1.PS00599 Full length( MysmA.00698.a ) 1 376

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.6472
RefSeq: YP_890856.1
UniProt: A0R6R8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTATESEAP RIHKGLAGVV VDTTAISKVV PETNSLTYRG YPVQDLAAQC SFEQVAYLLW HGELPTDQQL ALFSQRERAS RRIDRSMQAL LAKLPDNCHP MDVVRTAISY LGAEDPEEDV DTAEANYAKS LRMFAVLPTI VATDIRRRQG LTPIPPHSQL GYAQNFLNMC FGEVPEPVVV RAFEQSMVLY AEHSFNASTF AARVVTSTQS DIYSAVTAAI GALKGSLHGG ANEAVMHDML EIGSAEKAPE WLHGKLSRKE KVMGFGHRVY KNGDSRVPTM KVALEQVAQV RDGQRWLDIY NTLESAMFAA TRIKPNLDFP TGPAYYLMDF PIESFTPLFV MSRITGWTAH IMEQAASNAL IRPLSEYSGQ PQRSLV
NT Sequence
ATGACCACAG CAACAGAATC CGAAGCTCCG CGCATTCACA AGGGACTTGC CGGTGTCGTC GTCGACACCA CCGCCATCTC GAAGGTCGTG CCCGAGACCA ACAGCCTGAC CTACCGGGGC TACCCGGTGC AGGACCTGGC CGCCCAGTGC AGCTTTGAAC AGGTCGCGTA TCTGTTGTGG CACGGCGAAC TGCCGACCGA TCAGCAGCTG GCTTTGTTCA GCCAGCGTGA GCGGGCGTCG CGGCGGATCG ACCGGTCGAT GCAGGCGCTG CTGGCGAAGT TGCCCGACAA CTGTCATCCC ATGGACGTGG TGCGTACGGC GATCAGCTAT CTGGGAGCCG AGGATCCAGA AGAGGACGTC GACACGGCGG AGGCCAACTA CGCCAAGTCG CTGCGGATGT TCGCCGTGCT GCCGACGATC GTCGCCACCG ACATCCGGCG TCGTCAGGGA TTGACCCCGA TCCCTCCGCA CAGCCAGCTC GGTTATGCGC AGAACTTCTT GAACATGTGC TTCGGTGAGG TGCCGGAGCC CGTCGTGGTG CGCGCGTTCG AACAGTCGAT GGTGCTCTAC GCTGAGCACA GCTTCAACGC CTCCACCTTC GCCGCCCGCG TCGTCACCTC GACCCAGTCA GACATCTACA GCGCGGTGAC CGCGGCGATC GGCGCTCTCA AGGGTTCGCT GCACGGCGGC GCCAACGAAG CGGTCATGCA CGACATGCTC GAAATCGGTT CGGCCGAAAA GGCTCCGGAG TGGTTGCACG GCAAACTATC CCGCAAGGAG AAGGTCATGG GATTTGGCCA CCGGGTCTAC AAGAACGGTG ATTCCCGGGT GCCGACCATG AAGGTTGCTT TGGAGCAGGT TGCCCAGGTG CGTGACGGAC AGCGTTGGCT CGACATCTAC AACACGCTGG AGAGCGCGAT GTTCGCAGCG ACGAGGATCA AGCCGAACCT CGACTTTCCC ACCGGCCCTG CCTATTACCT GATGGACTTC CCGATCGAGA GTTTCACCCC GCTGTTCGTG ATGAGCCGGA TCACCGGTTG GACCGCCCAC ATCATGGAAC AGGCTGCCTC CAATGCTCTG ATCCGTCCCC TGAGTGAGTA CTCGGGCCAG CCGCAGCGGT CCCTTGTC
Details for MysmA.00698.a.A1.PS00599
PURIFICATION DATe: 5/13/2010
CONCENTRATION: 21.18mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 87
PERCENT COVERAGE: 75
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTTATESEA PRIHKGLAGV VVDTTAISKV VPETNSLTYR GYPVQDLAAQ CSFEQVAYLL WHGELPTDQQ LALFSQRERA SRRIDRSMQA LLAKLPDNCH PXDVVRTAIS YLGAEDPEED VDTAEANYAK SLRMFAVLPT IVATDIRRRQ GLTPIPPHSQ LGYAQNFLNM CFGEVPEPVV VRAFEQSMVL YAEHSFNAST FAARVVTSTX XDIYXAVTAA IXALKGSLHG GAXEXXMHXX LXXXSAERLR XXCTANYPAR RRSWXLATGS TRTVIPGCRP
Validated NT Sequence
attttnttta ctttaagaag gagatntacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacca cagcaacaga atccgaagct ccgcgcattc acaagggact tgccggtgtc gtcgtcgaca ccaccgccat ctcgaaggtc gtgcccgaga ccaacagcct gacctaccgg ggctacccgg tgcaggacct ggccgcccag tgcagctttg aacaggtcgc gtatctgttg tggcacggcg aactgccgac cgatcagcag ctggctttgt tcagccagcg tgagcgggcg tcgcggcgga tcgaccggtc gatgcaggcg ctgctggcga agttgcccga caactgtcat ccannggacg tggtgcgtac ggcgatcagc tatctgggag ccgaggatcc agaagaggac gtcgacacgg cggaggccaa ctacgccaag tcgctgcgga tgttcgccgt gctgccgacg atcgtcgcca ccgacatccg gcgtcgtcag ggattgaccc cgatccctcc gcacagccag ctcggttatg cgcagaactt cttgaacatg tgcttcggtg aggtgccgga gcccgtcgtg gtgcgcgcgt tcgaacagtc gatggtgctc tacgctgagc acagcttcaa cgcctccacc ttcgccgccc gcgtcgtcac ctcnacnnnn ncagacatct acagngcggt gaccgcggcg atcgncgctc tcaagggttc gctgcacggc ggcgccnacg aancgnncat gcacganntg ctcnaantcg nttcggccga aaggctccgg antgnntgca cggcaaacta tcccgcaagg agaaggtcat gggntttggc caccgggtct acaagaacgg tgattcccgg gtgccgacca tgaaggttgc tttnnannnn nntgccnnng tgcgtgacgg nnngcgnnng ctcgacatcn acanacgctg ganancgnga ngtnncancn acnannatna gcnancnnng antttcnnnn nngnccnnnn tnntncnnga ngnnnnnnat cgnnnnntnc nccccncnnn nnnnannanc nnnannnnnn nnnnncnncn nnangnnnnn gntncnnnnn nnnnnnnnnn cnnnncnnnn nnnncnnnnn nnnnnnnnnn nnnnnnaann nnnnnnnnnn nnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTATESEA PRIHKGLAGV VVDTTAISKV VPETNSLTYR GYPVQDLAAQ CSFEQVAYLL WHGELPTDQQ LALFSQRERA SRRIDRSMQA LLAKLPDNCH PMDVVRTAIS YLGAEDPEED VDTAEANYAK SLRMFAVLPT IVATDIRRRQ GLTPIPPHSQ LGYAQNFLNM CFGEVPEPVV VRAFEQSMVL YAEHSFNAST FAARVVTSTQ SDIYSAVTAA IGALKGSLHG GANEAVMHDM LEIGSAEKAP EWLHGKLSRK EKVMGFGHRV YKNGDSRVPT MKVALEQVAQ VRDGQRWLDI YNTLESAMFA ATRIKPNLDF PTGPAYYLMD FPIESFTPLF VMSRITGWTA HIMEQAASNA LIRPLSEYSG QPQRSLV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCACAGCA ACAGAATCCG AAGCTCCGCG CATTCACAAG GGACTTGCCG GTGTCGTCGT CGACACCACC GCCATCTCGA AGGTCGTGCC CGAGACCAAC AGCCTGACCT ACCGGGGCTA CCCGGTGCAG GACCTGGCCG CCCAGTGCAG CTTTGAACAG GTCGCGTATC TGTTGTGGCA CGGCGAACTG CCGACCGATC AGCAGCTGGC TTTGTTCAGC CAGCGTGAGC GGGCGTCGCG GCGGATCGAC CGGTCGATGC AGGCGCTGCT GGCGAAGTTG CCCGACAACT GTCATCCCAT GGACGTGGTG CGTACGGCGA TCAGCTATCT GGGAGCCGAG GATCCAGAAG AGGACGTCGA CACGGCGGAG GCCAACTACG CCAAGTCGCT GCGGATGTTC GCCGTGCTGC CGACGATCGT CGCCACCGAC ATCCGGCGTC GTCAGGGATT GACCCCGATC CCTCCGCACA GCCAGCTCGG TTATGCGCAG AACTTCTTGA ACATGTGCTT CGGTGAGGTG CCGGAGCCCG TCGTGGTGCG CGCGTTCGAA CAGTCGATGG TGCTCTACGC TGAGCACAGC TTCAACGCCT CCACCTTCGC CGCCCGCGTC GTCACCTCGA CCCAGTCAGA CATCTACAGC GCGGTGACCG CGGCGATCGG CGCTCTCAAG GGTTCGCTGC ACGGCGGCGC CAACGAAGCG GTCATGCACG ACATGCTCGA AATCGGTTCG GCCGAAAAGG CTCCGGAGTG GTTGCACGGC AAACTATCCC GCAAGGAGAA GGTCATGGGA TTTGGCCACC GGGTCTACAA GAACGGTGAT TCCCGGGTGC CGACCATGAA GGTTGCTTTG GAGCAGGTTG CCCAGGTGCG TGACGGACAG CGTTGGCTCG ACATCTACAA CACGCTGGAG AGCGCGATGT TCGCAGCGAC GAGGATCAAG CCGAACCTCG ACTTTCCCAC CGGCCCTGCC TATTACCTGA TGGACTTCCC GATCGAGAGT TTCACCCCGC TGTTCGTGAT GAGCCGGATC ACCGGTTGGA CCGCCCACAT CATGGAACAG GCTGCCTCCA ATGCTCTGAT CCGTCCCCTG AGTGAGTACT CGGGCCAGCC GCAGCGGTCC CTTGTCAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA