MysmA.00681.a
Alanine dehydrogenase/pyridine nucleotide transhydrogenase
| CENTER ID: | MysmA.00681.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
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Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00681.a.A1.PS00596 | Full length( MysmA.00681.a ) | 1 | 360 |
Structures
3P2Y
DEPOSITED: 10/4/2010
DETERMINATION: XRay
CLONE: MysmA.00681.a.A1.GE28826
PROTEIN: MysmA.00681.a.A1.PS00596
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127241 |
| PATRIC ID: | fig|246196.19.peg.149 |
| RefSeq: | YP_884568.1 |
| UniProt: | A0QNT0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLIGVPRES AEGERRVALV PKVVEKLSAR GLEVVVESAA GAGALFSDAD YERAGATIGD PWPADVVVKV NPPTSDEISQ LKPGSVLIGF LAPRTQPELA SRLRIADVTA FAMESIPRIS RAQTMDALSS QANVAGYKAV LLGASLSTRF VPMLTTAAGT VKPASALVLG VGVAGLQALA TAKRLGAKTT GYDVRPEVAE QVRSVGAQWL DLGIDAAGEG GYARELSEAE RAQQQQALED AITKFDIVIT TALVPGRPAP RLVTAAAATG MQPGSVVVDL AGETGGNCEL TEPGRTIVHH GVTITSPLNL PATMPEHASE LYAKNVTALL DLLLTDDGVA PDFTDEIVAA SCITRTEGDI
NT Sequence
ATGACGTTGA TCGGAGTGCC GCGGGAGTCC GCGGAGGGCG AGCGCCGCGT CGCGCTTGTC CCAAAAGTCG TCGAGAAATT GAGTGCCCGA GGGCTTGAGG TCGTCGTGGA GAGTGCGGCC GGAGCGGGCG CGCTGTTCAG CGACGCGGAT TACGAGCGAG CGGGGGCCAC GATCGGTGAT CCGTGGCCGG CAGATGTGGT GGTGAAGGTG AATCCACCCA CGTCGGACGA GATCAGCCAG CTCAAGCCCG GTTCGGTACT CATCGGCTTC CTGGCCCCGC GGACACAGCC CGAGCTCGCG TCGCGGCTGC GCATCGCAGA TGTCACAGCC TTTGCGATGG AATCGATTCC GCGCATATCC CGTGCGCAGA CCATGGACGC GTTGTCGTCT CAGGCCAACG TCGCCGGGTA CAAGGCCGTG CTGTTGGGAG CGTCGCTGTC GACACGGTTC GTCCCGATGC TGACCACCGC GGCGGGAACG GTCAAACCCG CCAGTGCGCT GGTACTCGGA GTCGGCGTCG CGGGCCTGCA GGCGCTGGCG ACCGCTAAAC GCTTGGGCGC CAAGACAACC GGATACGACG TGCGCCCCGA GGTCGCCGAA CAGGTCCGTT CGGTCGGCGC GCAGTGGCTC GATCTGGGGA TCGACGCCGC AGGCGAGGGC GGGTACGCGC GTGAACTCAG CGAGGCCGAA CGTGCGCAAC AGCAGCAGGC GCTCGAGGAC GCGATCACCA AGTTCGACAT CGTGATCACG ACCGCGCTGG TGCCCGGCCG TCCCGCACCG CGTCTGGTGA CCGCCGCCGC GGCCACCGGT ATGCAGCCGG GAAGCGTCGT CGTCGATCTC GCCGGCGAGA CCGGCGGCAA CTGTGAGCTC ACCGAGCCGG GACGCACCAT CGTCCACCAC GGCGTCACGA TCACCTCGCC GCTGAACCTG CCCGCGACCA TGCCCGAGCA CGCCAGCGAG TTGTACGCCA AGAACGTGAC CGCCCTGCTC GATCTGCTGC TCACCGACGA CGGCGTCGCC CCCGATTTCA CCGACGAGAT CGTCGCTGCC TCCTGCATCA CCCGCACCGA AGGGGACATC
Details for MysmA.00681.a.A1.PS00596
| PURIFICATION DATe: | 5/11/2010 |
| CONCENTRATION: | 21.61mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 56 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTLIGVPRE SAEGERRVAL VPKVVEKLSA RGLEVVVESA AGAGALFSDA DYERAGATIG DPWPADVVVK VNPPXSDEIS QLKPGSVLIG FLAPRTQPEL XSXLRIADVT PFAMESIPRI SRAQTMDALX SQANVAGYXX VLLGAXLSXR FXPMLTTAAG TVKPAXALVL XVGVAGLXAL AXXXRXXXXT TGXXXXPXXX NXXXRXXRXG SIWXXXPQXX XXXRXXSXSP TXXTXXXXXX XDHQVRHRXX TALVPXRPXX XSGXXXPATX XXXXEASXSX SXXXXXXHXX XTXXXRPSSX XXXXXXXXXX LXXXXXXXXX XXXXXXXXXX XPXXXXPXXX XXXXXXXXXX XXX
Validated NT Sequence
tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgacgtt gatcggagtg ccgcgggagt ccgcggaggg cgagcgccgc gtcgcgcttg tcccaaaagt cgtcgagaaa ttgagtgccc gagggcttga ggtcgtcgtg gagagtgcgg ccggagcggg cgcgctgttc agcgacgcgg attacgagcg agcgggggcc acgatcggtg atccgtggcc ggcagatgtg gtggtgaagg tgaatccacc annntcggac gagatcagcc agctcaagcc cggttcggta ctcatcggct tcctggcccc gcggacacag cccgagctcg ngtcgnggct gcgcatcgca gatgtcacac cctttgcgat ggaatcgatt ccncgcatat cccgtgcgca gaccatggac gcgttgncnt ctcaggccaa cgtcgccggg tacnaggncg tgctgctggg agcgncgctg tcnanacgnt tcgncccgat gctgaccacc gcggcgggaa cggtcaaacc cgccngtgcg ctggtactcg nagtcggngt cgcgggnctg nnggcnctgg cnancnnnaa ncgctngnnc gncnanacaa ccggatnnna cntgngnccn agntnnnnna acangnncnt ncgnncgnnn cgcannggct cgatctgggn annnnnnccn caggnnnnnn nnnnnncncg cntgnnntca ncgagcccaa cgtgnnnaac annnnnnnnn ncnnnnnnnn ncgatcacca agttcgacat cgnntcncna ccgcnctggt gcccngccgt ccccncannn cgtctgggnn nnncgncccn gccacngnnn ntgnnnctcn ggaagcgtcn nngtcgatnt cgncnngcan nnccnnnngg cactgnnngn tcaccnagnc gnnncgacca tcntcancan ngngcnnnga nnnntnnncn gntnancnct tgncnnannn nnnnnnnnan nttgnncnnc nnnancnnnn cncnntntnn anctgntgnn naccnagnnn gnnnncnccc cnantnncna nnnnntncnt nnnnnnnnnc nnnntnncnn nnnnnnnnng g
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTLIGVPRE SAEGERRVAL VPKVVEKLSA RGLEVVVESA AGAGALFSDA DYERAGATIG DPWPADVVVK VNPPTSDEIS QLKPGSVLIG FLAPRTQPEL ASRLRIADVT AFAMESIPRI SRAQTMDALS SQANVAGYKA VLLGASLSTR FVPMLTTAAG TVKPASALVL GVGVAGLQAL ATAKRLGAKT TGYDVRPEVA EQVRSVGAQW LDLGIDAAGE GGYARELSEA ERAQQQQALE DAITKFDIVI TTALVPGRPA PRLVTAAAAT GMQPGSVVVD LAGETGGNCE LTEPGRTIVH HGVTITSPLN LPATMPEHAS ELYAKNVTAL LDLLLTDDGV APDFTDEIVA ASCITRTEGD I
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGTTGATC GGAGTGCCGC GGGAGTCCGC GGAGGGCGAG CGCCGCGTCG CGCTTGTCCC AAAAGTCGTC GAGAAATTGA GTGCCCGAGG GCTTGAGGTC GTCGTGGAGA GTGCGGCCGG AGCGGGCGCG CTGTTCAGCG ACGCGGATTA CGAGCGAGCG GGGGCCACGA TCGGTGATCC GTGGCCGGCA GATGTGGTGG TGAAGGTGAA TCCACCCACG TCGGACGAGA TCAGCCAGCT CAAGCCCGGT TCGGTACTCA TCGGCTTCCT GGCCCCGCGG ACACAGCCCG AGCTCGCGTC GCGGCTGCGC ATCGCAGATG TCACAGCCTT TGCGATGGAA TCGATTCCGC GCATATCCCG TGCGCAGACC ATGGACGCGT TGTCGTCTCA GGCCAACGTC GCCGGGTACA AGGCCGTGCT GTTGGGAGCG TCGCTGTCGA CACGGTTCGT CCCGATGCTG ACCACCGCGG CGGGAACGGT CAAACCCGCC AGTGCGCTGG TACTCGGAGT CGGCGTCGCG GGCCTGCAGG CGCTGGCGAC CGCTAAACGC TTGGGCGCCA AGACAACCGG ATACGACGTG CGCCCCGAGG TCGCCGAACA GGTCCGTTCG GTCGGCGCGC AGTGGCTCGA TCTGGGGATC GACGCCGCAG GCGAGGGCGG GTACGCGCGT GAACTCAGCG AGGCCGAACG TGCGCAACAG CAGCAGGCGC TCGAGGACGC GATCACCAAG TTCGACATCG TGATCACGAC CGCGCTGGTG CCCGGCCGTC CCGCACCGCG TCTGGTGACC GCCGCCGCGG CCACCGGTAT GCAGCCGGGA AGCGTCGTCG TCGATCTCGC CGGCGAGACC GGCGGCAACT GTGAGCTCAC CGAGCCGGGA CGCACCATCG TCCACCACGG CGTCACGATC ACCTCGCCGC TGAACCTGCC CGCGACCATG CCCGAGCACG CCAGCGAGTT GTACGCCAAG AACGTGACCG CCCTGCTCGA TCTGCTGCTC ACCGACGACG GCGTCGCCCC CGATTTCACC GACGAGATCG TCGCTGCCTC CTGCATCACC CGCACCGAAG GGGACATCAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA