SSGCID: Target Page

MysmA.00665.a

ABC transporter ATP-binding protein

CENTER ID:	MysmA.00665.a
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00665.a.A1.GE28824	Full length( MysmA.00665.a )		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|246196.19.peg.3701
RefSeq:	YP_888056.1
UniProt:	A0QYR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMTSSRDELS ATPSEREPAI EIDGLHVVRG GRPAVRDVTV RIARGTITGL LGPSGCGKTT LMRCIVGTQI VAKGTVTVLG HPAGSAPLRH RVGYVTQNPT IYDDLRVIDN VRYFAALYGT SPAAADQAVD AVGLGDQRSA YCGNLSGGQR TRASLACALV AEPELLVLDE PTVGLDPVLR VDLWQQFNDL AAKGTTLVVS SHVMDEADHC GDLLLMREGR LLAHTTPARL REDTSCSSLE QAFLAVIRQY AAAA

NT Sequence

ATGATGACTT CATCGCGCGA TGAATTGTCA GCGACGCCGA GCGAGCGGGA GCCCGCGATC GAGATCGACG GATTGCACGT CGTGCGCGGC GGCAGACCCG CGGTGCGCGA CGTCACCGTC CGCATCGCGC GCGGCACCAT CACCGGCCTG CTCGGCCCGT CGGGCTGCGG CAAGACGACT CTCATGCGCT GCATCGTCGG CACCCAGATC GTCGCCAAAG GGACGGTCAC GGTGCTCGGC CATCCTGCGG GCTCGGCCCC GCTGCGCCAC CGCGTCGGTT ACGTGACGCA GAACCCCACG ATCTACGACG ACCTGCGCGT CATCGACAAC GTCCGCTACT TCGCGGCGCT GTACGGCACC TCCCCCGCTG CCGCCGATCA GGCCGTCGAC GCGGTCGGCC TCGGCGACCA GCGCAGCGCA TACTGCGGCA ATCTCTCGGG AGGCCAGCGC ACACGCGCGT CGCTCGCATG TGCGCTGGTC GCCGAACCCG AACTGCTGGT CCTCGACGAG CCGACCGTCG GACTCGACCC GGTCCTCCGC GTCGATCTGT GGCAGCAGTT CAACGACCTC GCCGCGAAAG GCACCACGCT TGTGGTCTCC AGCCACGTCA TGGACGAGGC GGATCACTGC GGTGACCTGC TGCTCATGCG CGAGGGCCGG CTCCTCGCCC ACACCACCCC GGCCCGGCTC CGAGAGGACA CCTCATGCAG TTCCCTGGAA CAAGCGTTCC TGGCCGTCAT CAGGCAGTAC GCCGCGGCAG CG

Details for MysmA.00665.a.A1.GE28824

HARVESTED ON:	3/23/2010
SEQUENCED ON:	4/13/2010
EXPECTED MW:	29kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	96

Validated AA Sequence

MGTLEXQTQG XGXMMTSSRD ELSATPSERE PAIEIDGLHV VRGGRPAVRD VTVRIARGTI TGLLGPSGCG KTTLMRCIVG TQIVAKGTVT VLGHPAGSAP LRHRVGYVTQ NPTIYDDLRV IDNVRYFAAL YGTSPAAADQ AVDAVGLGDQ RSAYCGNLSG GQRTRASLAC ALVAEPELLV LDEPTVGLDP VLRVDLWQQF NDLAAKGTTL VVSSHVMDEA DHCGDLLLMR EGRLLAHTTP ARLREDTSCS SLEQAFLAVI RQYAAA

Validated NT Sequence

anggctcatc accatcacca tcatatgggt accctggaag ntcagaccca gggtcnnggt tngatgatga cttcatcgcg cgatgaattg tcagcgacgc cgagcgagcg ggagcccgcg atcgagatcg acggattgca cgtcgtgcgc ggcggcagac ccgcggtgcg cgacgtcacc gtccgcatcg cgcgcggcac catcaccggc ctgctcggcc cgtcgggctg cggcaagacg actctcatgc gctgcatcgt cggcacccag atcgtcgcca aagggacggt cacggtgctc ggccatcctg cgggctcggc cccgctgcgc caccgcgtcg gttacgtgac gcagaacccc acgatctacg acgacctgcg cgtcatcgac aacgtccgct acttcgcggc gctgtacggc acctcccccg ctgccgccga tcaggccgtc gacgcggtcg gcctcggcga ccagcgcagc gcatactgcg gcaatctctc gggaggccag cgcacacgcg cgtcgctcgc atgtgcgctg gtcgccgaac ccgaactgct ggtcctcgac gagccgaccg tcggactcga cccggtcctc cgcgtcgatc tgtggcagca gttcaacgac ctcgccgcga aaggcaccac gcttgtggtc tccagccacg tcatggacga ggcggatcac tgcggtgacc tgctgctcat gcgcgagggc cggctcctcg cccacaccac cccggcccgg ctccgagagg acacctcatg cagttccctg gaacaagcgt tcctggccgt catcaggcag tacgccgcgg cagcg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMMTSSRDEL SATPSEREPA IEIDGLHVVR GGRPAVRDVT VRIARGTITG LLGPSGCGKT TLMRCIVGTQ IVAKGTVTVL GHPAGSAPLR HRVGYVTQNP TIYDDLRVID NVRYFAALYG TSPAAADQAV DAVGLGDQRS AYCGNLSGGQ RTRASLACAL VAEPELLVLD EPTVGLDPVL RVDLWQQFND LAAKGTTLVV SSHVMDEADH CGDLLLMREG RLLAHTTPAR LREDTSCSSL EQAFLAVIRQ YAAAA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gatgacttca tcgcgcgatg aattgtcagc gacgccgagc gagcgggagc ccgcgatcga gatcgacgga ttgcacgtcg tgcgcggcgg cagacccgcg gtgcgcgacg tcaccgtccg catcgcgcgc ggcaccatca ccggcctgct cggcccgtcg ggctgcggca agacgactct catgcgctgc atcgtcggca cccagatcgt cgccaaaggg acggtcacgg tgctcggcca tcctgcgggc tcggccccgc tgcgccaccg cgtcggttac gtgacgcaga accccacgat ctacgacgac ctgcgcgtca tcgacaacgt ccgctacttc gcggcgctgt acggcacctc ccccgctgcc gccgatcagg ccgtcgacgc ggtcggcctc ggcgaccagc gcagcgcata ctgcggcaat ctctcgggag gccagcgcac acgcgcgtcg ctcgcatgtg cgctggtcgc cgaacccgaa ctgctggtcc tcgacgagcc gaccgtcgga ctcgacccgg tcctccgcgt cgatctgtgg cagcagttca acgacctcgc cgcgaaaggc accacgcttg tggtctccag ccacgtcatg gacgaggcgg atcactgcgg tgacctgctg ctcatgcgcg agggccggct cctcgcccac accaccccgg cccggctccg agaggacacc tcatgcagtt ccctggaaca agcgttcctg gccgtcatca ggcagtacgc cgcggcagcg aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa