MysmA.00629.c

2,3-dihydroxybenzoate-AMP ligase (MbtA)

CENTER ID:	MysmA.00629.c
ORGANISM:	Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00629.c.B1.GE41217	full length		1	558
MysmA.00629.c.B2.GE39512	ordered domain		24	558

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MysmA.00629.c.B1.PS38261	full length		1	558
MysmA.00629.c.KP1.PS38350	full length		1	558

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|246196.19.peg.4419
RefSeq:	YP_888788.1
UniProt:	A0R0V0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTLTTPHPRP EQSESAAQSS LLAGFTPFPA ERAQAYRAAG YWRDQLLDSV LRTAARTWPD HIAVIDADHR HTYAELDRLA DRAAAGIAGL GIRPGDRVLV QLPNTAEFAV ALFGLLRAGA VPVMCLPGHR LAELTHFAEV SSAVALVVAD TAGGFDHRDL ARELVRSHPD VRHVLVDGDA AEFLSWAEVT RAAPGPVPEI APDPAAPALL LVSGGTTGAP KLIPRTHQDY VYNATASAEL CRLTADDVYL VALPAAHNFP LACPGLLGAM TVGATTVFTT DPSPEAAFAA IDEHGVTATA LVPALAKLWA QACAWEPLAP KTLRLLQVGG AKLAAPDAAL VRGALTPGLQ QVFGMAEGLL NYTRIGDPPE VLENTQGRPL SPDDEIRIVD EVGNEVPPGA EGELLVRGPY TLNGYFNAEA ANERSFSPDG FYRSGDRVRR FADGPLAGYL EVTGRIKDVI VRGGENVSAL DLEEHLLTHP SVWAAAAVAL PDEFLGEKIC AVVVFNGPPV SLAELHAHLE QRGVAAHSRP DALVPMPSLP TTAVGKIDKK AIVRQLGG

NT Sequence

gtgactctga ccacgcccca cccccgcccc gagcaatccg aatctgcagc tcagtcaagc ctgctggccg gattcacgcc gtttccggcc gagcgcgcac aggcctatcg ggcggccggg tactggcggg accagctact ggattcggtc ctgcggacgg cggcccgcac atggccggac cacatcgcgg tgatcgatgc ggaccaccgg cacacctacg ccgaactcga ccgcctcgcc gaccgcgccg cggcaggtat cgcgggcctg ggcatccgcc ccggcgaccg tgtgctggtc cagctgccca acaccgcgga gttcgccgtc gcactgttcg gcctgctgcg cgcgggcgcg gtacccgtga tgtgtctgcc cgggcaccgg ctggccgagc tcacgcattt cgccgaggtc agctcggcgg tggcgctcgt cgtggccgac accgcaggcg gtttcgacca ccgcgacctt gcacgcgaac tcgtgaggtc ccatccggac gtgcgtcacg tcctcgtcga cggcgacgcc gcggagttcc tgtcctgggc ggaggtgacg cgtgcggcgc ccgggcccgt ccccgagatc gcgcccgatc cggccgcccc ggcgctgctg ctcgtgtcgg gcggcaccac cggtgcaccg aaactcatcc cgcgcacaca tcaggactac gtctacaacg ccacggccag tgccgagctg tgccggctga ccgccgacga cgtctatctc gtggcgctgc ctgccgccca caacttccca ctggcgtgcc ccggcctgct cggtgcgatg accgtcggtg ccaccacggt gttcaccacc gacccgagcc ccgaggccgc gttcgccgcg atcgacgagc acggcgtcac ggccaccgca ctggtgcctg cgctggccaa gctgtgggcg caggcgtgtg cgtgggaacc cttggctccc aagacgctgc gcctgctcca ggtgggcggg gccaagctgg ctgcgccgga cgcggcgctg gtgcgcgggg ccctcacccc gggactgcag caggtgttcg gcatggccga gggcctgctg aactacacgc gcataggcga tccccccgag gtgctggaga acacccaggg ccgcccgttg tcaccggacg acgagatccg catcgtcgac gaggtcggca acgaggtgcc gcccggcgcc gagggggaac tgctggtgcg cggaccgtac acgctcaacg gctatttcaa cgccgaggcg gccaacgagc gctcgttcag cccggacggc ttctaccgca gcggcgaccg ggtacgccgc ttcgccgacg gcccgctcgc cgggtatctg gaggtgaccg gccggatcaa ggacgtcatc gtgcgcggcg gcgagaacgt ctccgcactc gatctcgaag agcacctgct gacccacccg agcgtgtggg ccgccgcagc ggttgcgctg cccgacgagt tcctcggcga gaagatctgc gccgtggtcg ttttcaacgg acccccggtc agcctggccg agttgcacgc gcatctggag cagcgcggcg tcgctgccca cagcaggccc gacgcgctgg tcccgatgcc gagcctgccc accacggccg tcggcaagat cgacaagaag gcgatcgtgc gtcagctcgg cgggtag

Details for MysmA.00629.c.B1.GE41217

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	60kDa
OBSERVED MW:	62kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT LTTPHPRPEQ SESAAQSSLL AGFTPFPAER AQAYRAAGYW RDQLLDSVLR TAARTWPDHI AVIDADHRHT YAELDRLADR AAAGIAGLGI RPGDRVLVQL PNTAEFAVAL FGLLRAGAVP VMCLPGHRLA ELTHFAEVSS AVALVVADTA GGFDHRDLAR ELVRSHPDVR HVLVDGDAAE FLSWAEVTRA APGPVPEIAP DPAAPALLLV SGGTTGAPKL IPRTHQDYVY NATASAELCR LTADDVYLVA LPAAHNFPLA CPGLLGAMTV GATTVFTTDP SPEAAFAAID EHGVTATALV PALAKLWAQA CAWEPLAPKT LRLLQVGGAK LAAPDAALVR GALTPGLQQV FGMAEGLLNY TRIGDPPEVL ENTQGRPLSP DDEIRIVDEV GNEVPPGAEG ELLVRGPYTL NGYFNAEAAN ERSFSPDGFY RSGDRVRRFA DGPLAGYLEV TGRIKDVIVR GGENVSALDL EEHLLTHPSV WAAAAVALPD EFLGEKICAV VVFNGPPVSL AELHAHLEQR GVAAHSRPDA LVPMPSLPTT AVGKIDKKAI VRQLGG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactc tgaccacgcc ccacccccgc cccgagcaat ccgaatctgc agctcagtca agcctgctgg ccggattcac gccgtttccg gccgagcgcg cacaggccta tcgggcggcc gggtactggc gggaccagct actggattcg gtcctgcgga cggcggcccg cacatggccg gaccacatcg cggtgatcga tgcggaccac cggcacacct acgccgaact cgaccgcctc gccgaccgcg ccgcggcagg tatcgcgggc ctgggcatcc gccccggcga ccgtgtgctg gtccagctgc ccaacaccgc ggagttcgcc gtcgcactgt tcggcctgct gcgcgcgggc gcggtacccg tgatgtgtct gcccgggcac cggctggccg agctcacgca tttcgccgag gtcagctcgg cggtggcgct cgtcgtggcc gacaccgcag gcggtttcga ccaccgcgac cttgcacgcg aactcgtgag gtcccatccg gacgtgcgtc acgtcctcgt cgacggcgac gccgcggagt tcctgtcctg ggcggaggtg acgcgtgcgg cgcccgggcc cgtccccgag atcgcgcccg atccggccgc cccggcgctg ctgctcgtgt cgggcggcac caccggtgca ccgaaactca tcccgcgcac acatcaggac tacgtctaca acgccacggc cagtgccgag ctgtgccggc tgaccgccga cgacgtctat ctcgtggcgc tgcctgccgc ccacaacttc ccactggcgt gccccggcct gctcggtgcg atgaccgtcg gtgccaccac ggtgttcacc accgacccga gccccgaggc cgcgttcgcc gcgatcgacg agcacggcgt cacggccacc gcactggtgc ctgcgctggc caagctgtgg gcgcaggcgt gtgcgtggga acccttggct cccaagacgc tgcgcctgct ccaggtgggc ggggccaagc tggctgcgcc ggacgcggcg ctggtgcgcg gggccctcac cccgggactg cagcaggtgt tcggcatggc cgagggcctg ctgaactaca cgcgcatagg cgatcccccc gaggtgctgg agaacaccca gggccgcccg ttgtcaccgg acgacgagat ccgcatcgtc gacgaggtcg gcaacgaggt gccgcccggc gccgaggggg aactgctggt gcgcggaccg tacacgctca acggctattt caacgccgag gcggccaacg agcgctcgtt cagcccggac ggcttctacc gcagcggcga ccgggtacgc cgcttcgccg acggcccgct cgccgggtat ctggaggtga ccggccggat caaggacgtc atcgtgcgcg gcggcgagaa cgtctccgca ctcgatctcg aagagcacct gctgacccac ccgagcgtgt gggccgccgc agcggttgcg ctgcccgacg agttcctcgg cgagaagatc tgcgccgtgg tcgttttcaa cggacccccg gtcagcctgg ccgagttgca cgcgcatctg gagcagcgcg gcgtcgctgc ccacagcagg cccgacgcgc tggtcccgat gccgagcctg cccaccacgg ccgtcggcaa gatcgacaag aaggcgatcg tgcgtcagct cggcgggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for MysmA.00629.c.B2.GE39512

HARVESTED ON:	9/10/2015
SEQUENCED ON:	9/16/2015
EXPECTED MW:	58kDa
OBSERVED MW:	58kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG FTPFPAERAQ AYRAAGYWRD QLLDSVLRTA ARTWPDHIAV IDADHRHTYA ELDRLADRAA AGIAGLGIRP GDRVLVQLPN TAEFAVALFG LLRAGAVPVM CLPGHRLAEL THFAEVSSAV ALVVADTAGG FDHRDLAREL VRSHPDVRHV LVDGDAAEFL SWAEVTRAAP GPVPEIAPDP AAPALLLVSG GTTGAPKLIP RTHQDYVYNA TASAELCRLT ADDVYLVALP AAHNFPLACP GLLGAMTVGA TTVFTTDPSP EAAFAAIDEH GVTATALVPA LXKLWAQACA WEPLAPKTLR LLQVGGAKLA APDAALVRGA LTPGLQQVFG MAEGLLNYTR IGDPPEVLEN TQGRPLSPDD EIRIVDEVGN EVPPGAEGEL LVRGPYTLNG YFNAEAANER SFSPDGFYRS GDRVRRFADG PLAGYLEVTG RIKDVIVRGG ENVSALDLEE HLLTHPSVWA AAAVALPDEF LGEKICAVVV FNGPPVSLAE LHAHLEQRGV AAHSRPDALV PMPSLPTTAV GKIDK

Validated NT Sequence

cttcttgtcg atcttgccga cggccgtggt gggcaggctc ggcatcggga ccagcgcgtc gggcctgctg tgggcagcga cgccgcgctg ctccagatgc gcgtgcaact cggccaggct gaccgggggt ccgttgaaaa cgaccacggc gcagatcttc tcgccgagga actcgtcggg cagcgcaacc gctgcggcgg cccacacgct cgggtgggtc agcaggtgct cttcgagatc gagtgcggag acgttctcgc cgccgcgcac gatgacgtcc ttgatccggc cggtcacctc cagatacccg gcgagcgggc cgtcggcgaa gcggcgtacc cggtcgccgc tgcggtagaa gccgtccggg ctgaacgagc gctcgttggc cgcctcggcg ttgaaatagc cgttgagcgt gtacggtccg cgcaccagca gttccccctc ggcgccgggc ggcacctcgt tgccgacctc gtcgacgatg cggatctcgt cgtccggtga caacgggcgg ccctgggtgt tctccagcac ctcgggggga tcgcctatgc gcgtgtagtt cagcaggccc tcggccatgc cgaacacctg ctgcagtccc ggggtgaggg ccccgcgcac cagcgccgcg tccggcgcag ccagcttggc cccgcccacc tggagcaggc gcagcgtctt gggagccaag ggttcccacg cacacgcctg cgcccacagc ttnnncagcg caggcaccag tgcggtggcc gtgacgccgt gctcgtcgat cgcggcgaac gcggcctcgg ggctcgggtc ggtggtgaac accgtggtgg caccgacggt catcgcaccg agcaggccgg ggcacgccag tgggaagttg tgggcggcag gcagcgccac gagatagacg tcgtcggcgg tcagccggca cagctcggca ctggccgtgg cgttgtagac gtagtcctga tgtgtgcgcg ggatgagttt cggtgcaccg gtggtgccgc ccgacacgag cagcagcgcc ggggcggccg gatcgggcgc gatctcgggg acgggcccgg gcgccgcacg cgtcacctcc gcccaggaca ggaactccgc ggcgtcgccg tcgacgagga cgtgacgcac gtccggatgg gacctcacga gttcgcgtgc aaggtcgcgg tggtcgaaac cgcctgcggt gtcggccacg acgagcgcca ccgccgagct gacctcggcg aaatgcgtga gctcggccag ccggtgcccg ggcagacaca tcacgggtac cgcgcccgcg cgcagcaggc cgaacagtgc gacggcgaac tccgcggtgt tgggcagctg gaccagcaca cggtcgccgg ggcggatgcc caggcccgcg atacctgccg cggcgcggtc ggcgaggcgg tcgagttcgg cgtaggtgtg ccggtggtcc gcatcgatca ccgcgatgtg gtccggccat gtgcgggccg ccgtccgcag gaccgaatcc agtagctggt cccgccagta cccggccgcc cgataggcct gtgcgcgctc ggccggaaac ggcgtgaatc ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHGF TPFPAERAQA YRAAGYWRDQ LLDSVLRTAA RTWPDHIAVI DADHRHTYAE LDRLADRAAA GIAGLGIRPG DRVLVQLPNT AEFAVALFGL LRAGAVPVMC LPGHRLAELT HFAEVSSAVA LVVADTAGGF DHRDLARELV RSHPDVRHVL VDGDAAEFLS WAEVTRAAPG PVPEIAPDPA APALLLVSGG TTGAPKLIPR THQDYVYNAT ASAELCRLTA DDVYLVALPA AHNFPLACPG LLGAMTVGAT TVFTTDPSPE AAFAAIDEHG VTATALVPAL AKLWAQACAW EPLAPKTLRL LQVGGAKLAA PDAALVRGAL TPGLQQVFGM AEGLLNYTRI GDPPEVLENT QGRPLSPDDE IRIVDEVGNE VPPGAEGELL VRGPYTLNGY FNAEAANERS FSPDGFYRSG DRVRRFADGP LAGYLEVTGR IKDVIVRGGE NVSALDLEEH LLTHPSVWAA AAVALPDEFL GEKICAVVVF NGPPVSLAEL HAHLEQRGVA AHSRPDALVP MPSLPTTAVG KIDKKAIVRQ LGG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggattca cgccgtttcc ggccgagcgc gcacaggcct atcgggcggc cgggtactgg cgggaccagc tactggattc ggtcctgcgg acggcggccc gcacatggcc ggaccacatc gcggtgatcg atgcggacca ccggcacacc tacgccgaac tcgaccgcct cgccgaccgc gccgcggcag gtatcgcggg cctgggcatc cgccccggcg accgtgtgct ggtccagctg cccaacaccg cggagttcgc cgtcgcactg ttcggcctgc tgcgcgcggg cgcggtaccc gtgatgtgtc tgcccgggca ccggctggcc gagctcacgc atttcgccga ggtcagctcg gcggtggcgc tcgtcgtggc cgacaccgca ggcggtttcg accaccgcga ccttgcacgc gaactcgtga ggtcccatcc ggacgtgcgt cacgtcctcg tcgacggcga cgccgcggag ttcctgtcct gggcggaggt gacgcgtgcg gcgcccgggc ccgtccccga gatcgcgccc gatccggccg ccccggcgct gctgctcgtg tcgggcggca ccaccggtgc accgaaactc atcccgcgca cacatcagga ctacgtctac aacgccacgg ccagtgccga gctgtgccgg ctgaccgccg acgacgtcta tctcgtggcg ctgcctgccg cccacaactt cccactggcg tgccccggcc tgctcggtgc gatgaccgtc ggtgccacca cggtgttcac caccgacccg agccccgagg ccgcgttcgc cgcgatcgac gagcacggcg tcacggccac cgcactggtg cctgcgctgg ccaagctgtg ggcgcaggcg tgtgcgtggg aacccttggc tcccaagacg ctgcgcctgc tccaggtggg cggggccaag ctggctgcgc cggacgcggc gctggtgcgc ggggccctca ccccgggact gcagcaggtg ttcggcatgg ccgagggcct gctgaactac acgcgcatag gcgatccccc cgaggtgctg gagaacaccc agggccgccc gttgtcaccg gacgacgaga tccgcatcgt cgacgaggtc ggcaacgagg tgccgcccgg cgccgagggg gaactgctgg tgcgcggacc gtacacgctc aacggctatt tcaacgccga ggcggccaac gagcgctcgt tcagcccgga cggcttctac cgcagcggcg accgggtacg ccgcttcgcc gacggcccgc tcgccgggta tctggaggtg accggccgga tcaaggacgt catcgtgcgc ggcggcgaga acgtctccgc actcgatctc gaagagcacc tgctgaccca cccgagcgtg tgggccgccg cagcggttgc gctgcccgac gagttcctcg gcgagaagat ctgcgccgtg gtcgttttca acggaccccc ggtcagcctg gccgagttgc acgcgcatct ggagcagcgc ggcgtcgctg cccacagcag gcccgacgcg ctggtcccga tgccgagcct gcccaccacg gccgtcggca agatcgacaa gaaggcgatc gtgcgtcagc tcggcgggtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for MysmA.00629.c.B1.PS38261

PURIFICATION DATe:	4/12/2017
CONCENTRATION:	30.51mg/ml
OBSERVED MW:	72kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACTC TGACCACGCC CCACCCCCGC CCCGAGCAAT CCGAATCTGC AGCTCAGTCA AGCCTGCTGG CCGGATTCAC GCCGTTTCCG GCCGAGCGCG CACAGGCCTA TCGGGCGGCC GGGTACTGGC GGGACCAGCT ACTGGATTCG GTCCTGCGGA CGGCGGCCCG CACATGGCCG GACCACATCG CGGTGATCGA TGCGGACCAC CGGCACACCT ACGCCGAACT CGACCGCCTC GCCGACCGCG CCGCGGCAGG TATCGCGGGC CTGGGCATCC GCCCCGGCGA CCGTGTGCTG GTCCAGCTGC CCAACACCGC GGAGTTCGCC GTCGCACTGT TCGGCCTGCT GCGCGCGGGC GCGGTACCCG TGATGTGTCT GCCCGGGCAC CGGCTGGCCG AGCTCACGCA TTTCGCCGAG GTCAGCTCGG CGGTGGCGCT CGTCGTGGCC GACACCGCAG GCGGTTTCGA CCACCGCGAC CTTGCACGCG AACTCGTGAG GTCCCATCCG GACGTGCGTC ACGTCCTCGT CGACGGCGAC GCCGCGGAGT TCCTGTCCTG GGCGGAGGTG ACGCGTGCGG CGCCCGGGCC CGTCCCCGAG ATCGCGCCCG ATCCGGCCGC CCCGGCGCTG CTGCTCGTGT CGGGCGGCAC CACCGGTGCA CCGAAACTCA TCCCGCGCAC ACATCAGGAC TACGTCTACA ACGCCACGGC CAGTGCCGAG CTGTGCCGGC TGACCGCCGA CGACGTCTAT CTCGTGGCGC TGCCTGCCGC CCACAACTTC CCACTGGCGT GCCCCGGCCT GCTCGGTGCG ATGACCGTCG GTGCCACCAC GGTGTTCACC ACCGACCCGA GCCCCGAGGC CGCGTTCGCC GCGATCGACG AGCACGGCGT CACGGCCACC GCACTGGTGC CTGCGCTGGC CAAGCTGTGG GCGCAGGCGT GTGCGTGGGA ACCCTTGGCT CCCAAGACGC TGCGCCTGCT CCAGGTGGGC GGGGCCAAGC TGGCTGCGCC GGACGCGGCG CTGGTGCGCG GGGCCCTCAC CCCGGGACTG CAGCAGGTGT TCGGCATGGC CGAGGGCCTG CTGAACTACA CGCGCATAGG CGATCCCCCC GAGGTGCTGG AGAACACCCA GGGCCGCCCG TTGTCACCGG ACGACGAGAT CCGCATCGTC GACGAGGTCG GCAACGAGGT GCCGCCCGGC GCCGAGGGGG AACTGCTGGT GCGCGGACCG TACACGCTCA ACGGCTATTT CAACGCCGAG GCGGCCAACG AGCGCTCGTT CAGCCCGGAC GGCTTCTACC GCAGCGGCGA CCGGGTACGC CGCTTCGCCG ACGGCCCGCT CGCCGGGTAT CTGGAGGTGA CCGGCCGGAT CAAGGACGTC ATCGTGCGCG GCGGCGAGAA CGTCTCCGCA CTCGATCTCG AAGAGCACCT GCTGACCCAC CCGAGCGTGT GGGCCGCCGC AGCGGTTGCG CTGCCCGACG AGTTCCTCGG CGAGAAGATC TGCGCCGTGG TCGTTTTCAA CGGACCCCCG GTCAGCCTGG CCGAGTTGCA CGCGCATCTG GAGCAGCGCG GCGTCGCTGC CCACAGCAGG CCCGACGCGC TGGTCCCGAT GCCGAGCCTG CCCACCACGG CCGTCGGCAA GATCGACAAG AAGGCGATCG TGCGTCAGCT CGGCGGGTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT

Details for MysmA.00629.c.KP1.PS38350

PURIFICATION DATe:	6/16/2017
CONCENTRATION:	15.65mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	50 mM Tris pH 8.0, 150 mM NaCl, 1 mM DTT
EXPRESSION HOST:	BL21 DE3
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

MTLTTPHPRP EQSESAAQSS LLAGFTPFPA ERAQAYRAAG YWRDQLLDSV LRTAARTWPD HIAVIDADHR HTYAELDRLA DRAAAGIAGL GIRPGDRVLV QLPNTAEFAV ALFGLLRAGA VPVMCLPGHR LAELTHFAEV SSAVALVVAD TAGGFDHRDL ARELVRSHPD VRHVLVDGDA AEFLSWAEVT RAAPGPVPEI APDPAAPALL LVSGGTTGAP KLIPRTHQDY VYNATASAEL CRLTADDVYL VALPAAHNFP LACPGLLGAM TVGATTVFTT DPSPEAAFAA IDEHGVTATA LVPALAKLWA QACAWEPLAX XTLRLLQVGG AKLAAPDAAL VRGALTPGLQ QVFGMAEGLL NYTRIGDPPE VLENTQGRPL SPDDEIRIVD EVGNEVPPGA EGELLVRGPY TLNGYFNAEA ANERSFSPDG FYRSGDRVRR FADGPLAGYL EVTGRIKDVI VRGGENVSAL DLEEHLLTHP SVWAAAAVAL PDEFLGEKIC AVVVFNGPPV SLAELHAHLE QRGVAAHSRP DALVPMPSLP TTAVGKIDKK AIVRQLGG

Validated NT Sequence

gngcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat gactctgacc acgccccacc cccgccccga gcaatccgaa tctgcagctc agtcaagcct gctggccgga ttcacgccgt ttccggccga gcgcgcacag gcctatcggg cggccgggta ctggcgggac cagctactgg attcggtcct gcggacggcg gcccgcacat ggccggacca catcgcggtg atcgatgcgg accaccggca cacctacgcc gaactcgacc gcctcgccga ccgcgccgcg gcaggtatcg cgggcctggg catccgcccc ggcgaccgtg tgctggtcca gctgcccaac accgcggagt tcgccgtcgc actgttcggc ctgctgcgcg cgggcgcggt acccgtgatg tgtctgcccg ggcaccggct ggccgagctc acgcatttcg ccgaggtcag ctcggcggtg gcgctcgtcg tggccgacac cgcaggcggt ttcgaccacc gcgaccttgc acgcgaactc gtgaggtccc atccggacgt gcgtcacgtc ctcgtcgacg gcgacgccgc ggagttcctg tcctgggcgg aggtgacgcg tgcggcgccc gggcccgtcc ccgagatcgc gcccgatccg gccgccccgg cgctgctgct cgtgtcgggc ggcaccaccg gtgcaccgaa actcatcccg cgcacacatc aggactacgt ctacaacgcc acggccagtg ccgagctgtg ccggctgacc gccgacgacg tctatctcgt ggcgctgcct gccgcccaca acttcccact ggcgtgcccc ggcctgctcg gtgcgatgac cgtcggtgcc accacggtgt tcaccaccga cccgagcccc gaggccgcgt tcgccgcgat cgacgagcac ggcgtcacgg ccaccgcact ggtgcctgcg ctggccaagc tgtgggcgca ggcgtgtgcg tgggaaccct tggctnnnnn gacgctgcgc ctgctccagg tgggcggggc caagctggct gcgccggacg cggcgctggt gcgcggggcc ctcaccccgg gactgcagca ggtgttcggc atggccgagg gcctgctgaa ctacacgcgc ataggcgatc cccccgaggt gctggagaac acccagggcc gcccgttgtc accggacgac gagatccgca tcgtcgacga ggtcggcaac gaggtgccgc ccggcgccga gggggaactg ctggtgcgcg gaccgtacac gctcaacggc tatttcaacg ccgaggcggc caacgagcgc tcgttcagcc cggacggctt ctaccgcagc ggcgaccggg tacgccgctt cgccgacggc ccgctcgccg ggtatctgga ggtgaccggc cggatcaagg acgtcatcgt gcgcggcggc gagaacgtct ccgcactcga tctcgaagag cacctgctga cccacccgag cgtgtgggcc gccgcagcgg ttgcgctgcc cgacgagttc ctcggcgaga agatctgcgc cgtggtcgtt ttcaacggac ccccggtcag cctggccgag ttgcacgcgc atctggagca gcgcggcgtc gctgcccaca gcaggcccga cgcgctggtc ccgatgccga gcctgcccac cacggccgtc ggcaagatcg acaagaaggc gatcgtgcgt cagctcggcg ggtga

Expressed Protein Sequence

MGSSHHHHHH SSGLVPRGSH MMTLTTPHPR PEQSESAAQS SLLAGFTPFP AERAQAYRAA GYWRDQLLDS VLRTAARTWP DHIAVIDADH RHTYAELDRL ADRAAAGIAG LGIRPGDRVL VQLPNTAEFA VALFGLLRAG AVPVMCLPGH RLAELTHFAE VSSAVALVVA DTAGGFDHRD LARELVRSHP DVRHVLVDGD AAEFLSWAEV TRAAPGPVPE IAPDPAAPAL LLVSGGTTGA PKLIPRTHQD YVYNATASAE LCRLTADDVY LVALPAAHNF PLACPGLLGA MTVGATTVFT TDPSPEAAFA AIDEHGVTAT ALVPALAKLW AQACAWEPLA PKTLRLLQVG GAKLAAPDAA LVRGALTPGL QQVFGMAEGL LNYTRIGDPP EVLENTQGRP LSPDDEIRIV DEVGNEVPPG AEGELLVRGP YTLNGYFNAE AANERSFSPD GFYRSGDRVR RFADGPLAGY LEVTGRIKDV IVRGGENVSA LDLEEHLLTH PSVWAAAAVA LPDEFLGEKI CAVVVFNGPP VSLAELHAHL EQRGVAAHSR PDALVPMPSL PTTAVGKIDK KAIVRQLGG

Full NT Sequence (Expression Vector + Insert)

TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCAGCA GCCATCATCA TCATCATCAC AGCAGCGGCC TGGTGCCGCG CGGCAGCCAT ATGGTGACTC TGACCACGCC CCACCCCCGC CCCGAGCAAT CCGAATCTGC AGCTCAGTCA AGCCTGCTGG CCGGATTCAC GCCGTTTCCG GCCGAGCGCG CACAGGCCTA TCGGGCGGCC GGGTACTGGC GGGACCAGCT ACTGGATTCG GTCCTGCGGA CGGCGGCCCG CACATGGCCG GACCACATCG CGGTGATCGA TGCGGACCAC CGGCACACCT ACGCCGAACT CGACCGCCTC GCCGACCGCG CCGCGGCAGG TATCGCGGGC CTGGGCATCC GCCCCGGCGA CCGTGTGCTG GTCCAGCTGC CCAACACCGC GGAGTTCGCC GTCGCACTGT TCGGCCTGCT GCGCGCGGGC GCGGTACCCG TGATGTGTCT GCCCGGGCAC CGGCTGGCCG AGCTCACGCA TTTCGCCGAG GTCAGCTCGG CGGTGGCGCT CGTCGTGGCC GACACCGCAG GCGGTTTCGA CCACCGCGAC CTTGCACGCG AACTCGTGAG GTCCCATCCG GACGTGCGTC ACGTCCTCGT CGACGGCGAC GCCGCGGAGT TCCTGTCCTG GGCGGAGGTG ACGCGTGCGG CGCCCGGGCC CGTCCCCGAG ATCGCGCCCG ATCCGGCCGC CCCGGCGCTG CTGCTCGTGT CGGGCGGCAC CACCGGTGCA CCGAAACTCA TCCCGCGCAC ACATCAGGAC TACGTCTACA ACGCCACGGC CAGTGCCGAG CTGTGCCGGC TGACCGCCGA CGACGTCTAT CTCGTGGCGC TGCCTGCCGC CCACAACTTC CCACTGGCGT GCCCCGGCCT GCTCGGTGCG ATGACCGTCG GTGCCACCAC GGTGTTCACC ACCGACCCGA GCCCCGAGGC CGCGTTCGCC GCGATCGACG AGCACGGCGT CACGGCCACC GCACTGGTGC CTGCGCTGGC CAAGCTGTGG GCGCAGGCGT GTGCGTGGGA ACCCTTGGCT CCCAAGACGC TGCGCCTGCT CCAGGTGGGC GGGGCCAAGC TGGCTGCGCC GGACGCGGCG CTGGTGCGCG GGGCCCTCAC CCCGGGACTG CAGCAGGTGT TCGGCATGGC CGAGGGCCTG CTGAACTACA CGCGCATAGG CGATCCCCCC GAGGTGCTGG AGAACACCCA GGGCCGCCCG TTGTCACCGG ACGACGAGAT CCGCATCGTC GACGAGGTCG GCAACGAGGT GCCGCCCGGC GCCGAGGGGG AACTGCTGGT GCGCGGACCG TACACGCTCA ACGGCTATTT CAACGCCGAG GCGGCCAACG AGCGCTCGTT CAGCCCGGAC GGCTTCTACC GCAGCGGCGA CCGGGTACGC CGCTTCGCCG ACGGCCCGCT CGCCGGGTAT CTGGAGGTGA CCGGCCGGAT CAAGGACGTC ATCGTGCGCG GCGGCGAGAA CGTCTCCGCA CTCGATCTCG AAGAGCACCT GCTGACCCAC CCGAGCGTGT GGGCCGCCGC AGCGGTTGCG CTGCCCGACG AGTTCCTCGG CGAGAAGATC TGCGCCGTGG TCGTTTTCAA CGGACCCCCG GTCAGCCTGG CCGAGTTGCA CGCGCATCTG GAGCAGCGCG GCGTCGCTGC CCACAGCAGG CCCGACGCGC TGGTCCCGAT GCCGAGCCTG CCCACCACGG CCGTCGGCAA GATCGACAAG AAGGCGATCG TGCGTCAGCT CGGCGGGGCG GCCGCACTCG AGCACCACCA CCACCACCAC TGAGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAT