MysmA.00612.a

Lysine--tRNA ligase LysS (EC 6.1.1.6) (Lysyl-tRNA synthetase)

CENTER ID: MysmA.00612.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00612.a.B1.GE40884 full length 1 518
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00612.a.B1.PW38256 full length 1 518

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.56.peg.6056
UniProt: I7FU37

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRSRVHLSWD FVTPADRDDT SQPQADIPEQ FRIRQEKRER LLAQGHDPYP VKVDRTHTLA ELRRAHPDLA ADTATGQIVG VSGRVVFARN SGKLCFATLQ EGDGTQLQVM ISLAQVGQEA LDSWKSDVDL GDIVFVHGEV ISSKRGELSV LADRWQMAAK ALRPLPVAHK EMSEEARVRQ RYVDLIVRPE ARTIARQRVA VVRAVRSALE RRGFLEVETP MLQTLAGGAA ARPFITHSNA LDIDLYLRIA PELFLKRCLV GGFEKVFELN RNFRNEGVDS THSPEFAMLE TYQAYGDYND SATVTRELIQ EVADEAIGTR QVPLPDGTVY DLDGEWDTVE MYPSLSEALG DEITPETPVE KLWQIADRLE VEIPRDRGYG HGKLIEELWE HTVGDKLWAP TFVRDFPVET TPLTRAHRSI PGVTEKWDLY VRKFELATGY SELIDPVIQR ERFEAQARAA AAGDDEAMVL DEDFLAALEF AMPPTTGTGM GIDRLMMALT GLSIRETVLF PIVRRHSN
NT Sequence
gtgcgttcac gcgttcattt aagctgggat ttcgtgaccc cagccgatcg tgacgacacg tcccagcccc aggccgatat ccccgagcag ttccgaatcc gtcaggagaa gcgggagcgg ctgctggcgc aggggcacga tccgtacccg gtgaaggtcg atcgcaccca cacgctggcc gaactgcgcc gcgcccaccc cgacctggcc gccgacaccg cgaccggcca gatcgtcggg gtctccggcc gcgtggtgtt tgcccgcaac tcgggcaagt tgtgcttcgc gaccctgcag gagggcgacg gcacccaact gcaggtgatg atcagcctcg ctcaggtggg ccaggaggca ctcgactcct ggaagagtga cgtggacctc ggtgacatcg tgttcgtcca cggcgaagtc atcagttcca agcgtggcga attgtcggtg ctggccgaca gatggcagat ggcggcaaag gcgctgcggc cacttccggt tgctcacaaa gaaatgagcg aagaagcgcg cgtgcgtcag cgctatgtcg acctcatcgt ccggcccgag gcccgcacca tcgcccgcca gcgcgtcgcg gtggtccgcg ctgtgcgttc ggcgctcgaa cggcgcggat tcctcgaggt cgagaccccg atgctgcaga cgctcgccgg cggtgccgcc gcgcgcccgt tcatcacgca ctccaatgcg ctcgacatcg atctgtacct gaggatcgcg ccggaactgt tcctcaagcg ctgcctcgtg ggcggatttg agaaggtttt cgagttgaat cgcaactttc gcaatgaggg tgtggattcc acacactcgc cggaattcgc gatgttggag acatatcagg cttacggtga ctacaacgat tccgcgaccg tcacccgaga acttattcaa gaggtcgccg acgaagcgat cggcacgcgt caggtgccat tgcccgatgg cacggtctac gatctcgacg gtgaatggga cacggtggaa atgtacccat cgctgtcgga ggcactcggt gacgagatca ctcccgagac cccggtcgag aagttgtggc agattgcgga tcgacttgag gtcgagattc cgcgtgaccg cggttacggt cacgggaaat tgatcgaaga actatgggaa cacacggtcg gtgacaagtt gtgggccccg acgttcgtgc gcgacttccc cgtcgagacg actccactca cccgagcgca tcgcagcatt ccaggggtca ccgagaagtg ggacctgtat gtgaggaaat tcgaactcgc cacgggatac tccgaactca tcgacccggt aatccagcgc gagcgtttcg aggcccaggc ccgcgccgcc gccgccggcg acgacgaggc aatggttctc gacgaggatt tcctggccgc attggagttc gcgatgccgc cgaccacggg aaccggaatg ggcatcgata ggttgatgat ggcgttgaca gggttgtcta ttagggagac cgttttgttc ccgattgttc ggcgtcacag caactga
Details for MysmA.00612.a.B1.GE40884
HARVESTED ON: 12/5/2016
SEQUENCED ON: 12/6/2016
EXPECTED MW: 59kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV RSRVHLSWDF VTPADRDDTS QPQADIPEQF RIRQEKRERL LAQGHDPYPV KVDRTHTLAE LRRAHPDLAA DTATGQIVGV SGRVVFARNS GKLCFATLQE GDGTQLQVMI SLAQVGQEAL DSWKSDVDLG DIVFVHGEVI SSKRGELSVL ADRWQMAAKA LRPLPVAHKE MSEEARVRQR YVDLIVRPEA RTIARQRVAV VRAVRSALER RGFLEVETPM LQTLAGGAAA RPFITHSNAL DIDLYLRIAP ELFLKRCLVG GFEKVFELNR NFRNEGVDST HSPEFAMXET YQAYGDYNDS ATVTRELIQE VADEAIGTRQ VPLPDGTVYD LDGEWDTVEM YPSLSEALGD EITPETPVEK LWQIADRLEV EIPRDRGYGH GKLIEELWEH TVGDKLWAPT FVRDFPVETT PLTRAHRSIP GVTEKWDLYV RKFELATGYS ELIDPVIQRE RFEAQARAAA AGDDEAMVLD EDFLAALEFA MPPTTGTGMG IDRLMMALTG LSIRETVLF
Validated NT Sequence
gggaacaaaa cggtctccct aatagacaac cctgtcaacg ccatcatcaa cctatcgatg cccattccgg ttcccgtggt cggcggcatc gcgaactcca atgcggccag gaaatcctcg tcgagaacca ttgcctcgtc gtcgccggcg gcggcggcgc gggcctgggc ctcgaaacgc tcgcgctgga ttaccgggtc gatgagttcg gagtatcccg tggcgagttc gaatttcctc acatacaggt cccacttctc ggtgacccct ggaatgctgc gatgcgctcg ggtgagtgga gtcgtctcga cggggaagtc gcgcacgaac gtcggggccc acaacttgtc accgaccgtg tgttcccata gttcttcgat caatttcccg tgaccgtaac cgcggtcacg cggaatctcg acctcaagtc gatccgcaat ctgccacaac ttctcgaccg gggtctcggg agtgatctcg tcaccgagtg cctccgacag cgatgggtac atttccaccg tgtcccattc accgtcgaga tcgtagaccg tgccatcggg caatggcacc tgacgcgtgc cgatcgcttc gtcggcgacc tcttgaataa gttctcgggt gacggtcgcg gaatcgttgt agtcaccgta agcctgatat gtctccnnca tcgcgaattc cggcgagtgt gtggaatcca caccctcatt gcgaaagttg cgattcaact cgaaaacctt ctcaaatccg cccacgaggc agcgcttgag gaacagttcc ggcgcgatcc tcaggtacag atcgatgtcg agcgcattgg agtgcgtgat gaacgggcgc gcggcggcac cgccggcgag cgtctgcagc atcggggtct cgacctcgag gaatccgcgc cgttcgagcg ccgaacgcac agcgcggacc accgcgacgc gctggcgggc gatggtgcgg gcctcgggcc ggacgatgag gtcgacatag cgctgacgca cgcgcgcttc ttcgctcatt tctttgtgag caaccggaag tggccgcagc gcctttgccg ccatctgcca tctgtcggcc agcaccgaca attcgccacg cttggaactg atgacttcgc cgtggacgaa cacgatgtca ccgaggtcca cgtcactctt ccaggagtcg agtgcctcct ggcccacctg agcgaggctg atcatcacct gcagttgggt gccgtcgccc tcctgcaggg tcgcgaagca caacttgccc gagttgcggg caaacaccac gcggccggag accccgacga tctggccggt cgcggtgtcg gcggccaggt cggggtgggc gcggcgcagt tcggccagcg tgtgggtgcg atcgaccttc accgggtacg gatcgtgccc ctgcgccagc agccgctccc gcttctcctg acggattcgg aactgctcgg ggatatcggc ctggggctgg gacgtgtcgt cacgatcggc tggggtcacg aaatcccagc ttaaatgaac gcgtgaacgc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMR SRVHLSWDFV TPADRDDTSQ PQADIPEQFR IRQEKRERLL AQGHDPYPVK VDRTHTLAEL RRAHPDLAAD TATGQIVGVS GRVVFARNSG KLCFATLQEG DGTQLQVMIS LAQVGQEALD SWKSDVDLGD IVFVHGEVIS SKRGELSVLA DRWQMAAKAL RPLPVAHKEM SEEARVRQRY VDLIVRPEAR TIARQRVAVV RAVRSALERR GFLEVETPML QTLAGGAAAR PFITHSNALD IDLYLRIAPE LFLKRCLVGG FEKVFELNRN FRNEGVDSTH SPEFAMLETY QAYGDYNDSA TVTRELIQEV ADEAIGTRQV PLPDGTVYDL DGEWDTVEMY PSLSEALGDE ITPETPVEKL WQIADRLEVE IPRDRGYGHG KLIEELWEHT VGDKLWAPTF VRDFPVETTP LTRAHRSIPG VTEKWDLYVR KFELATGYSE LIDPVIQRER FEAQARAAAA GDDEAMVLDE DFLAALEFAM PPTTGTGMGI DRLMMALTGL SIRETVLFPI VRRHSN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgcgtt cacgcgttca tttaagctgg gatttcgtga ccccagccga tcgtgacgac acgtcccagc cccaggccga tatccccgag cagttccgaa tccgtcagga gaagcgggag cggctgctgg cgcaggggca cgatccgtac ccggtgaagg tcgatcgcac ccacacgctg gccgaactgc gccgcgccca ccccgacctg gccgccgaca ccgcgaccgg ccagatcgtc ggggtctccg gccgcgtggt gtttgcccgc aactcgggca agttgtgctt cgcgaccctg caggagggcg acggcaccca actgcaggtg atgatcagcc tcgctcaggt gggccaggag gcactcgact cctggaagag tgacgtggac ctcggtgaca tcgtgttcgt ccacggcgaa gtcatcagtt ccaagcgtgg cgaattgtcg gtgctggccg acagatggca gatggcggca aaggcgctgc ggccacttcc ggttgctcac aaagaaatga gcgaagaagc gcgcgtgcgt cagcgctatg tcgacctcat cgtccggccc gaggcccgca ccatcgcccg ccagcgcgtc gcggtggtcc gcgctgtgcg ttcggcgctc gaacggcgcg gattcctcga ggtcgagacc ccgatgctgc agacgctcgc cggcggtgcc gccgcgcgcc cgttcatcac gcactccaat gcgctcgaca tcgatctgta cctgaggatc gcgccggaac tgttcctcaa gcgctgcctc gtgggcggat ttgagaaggt tttcgagttg aatcgcaact ttcgcaatga gggtgtggat tccacacact cgccggaatt cgcgatgttg gagacatatc aggcttacgg tgactacaac gattccgcga ccgtcacccg agaacttatt caagaggtcg ccgacgaagc gatcggcacg cgtcaggtgc cattgcccga tggcacggtc tacgatctcg acggtgaatg ggacacggtg gaaatgtacc catcgctgtc ggaggcactc ggtgacgaga tcactcccga gaccccggtc gagaagttgt ggcagattgc ggatcgactt gaggtcgaga ttccgcgtga ccgcggttac ggtcacggga aattgatcga agaactatgg gaacacacgg tcggtgacaa gttgtgggcc ccgacgttcg tgcgcgactt ccccgtcgag acgactccac tcacccgagc gcatcgcagc attccagggg tcaccgagaa gtgggacctg tatgtgagga aattcgaact cgccacggga tactccgaac tcatcgaccc ggtaatccag cgcgagcgtt tcgaggccca ggcccgcgcc gccgccgccg gcgacgacga ggcaatggtt ctcgacgagg atttcctggc cgcattggag ttcgcgatgc cgccgaccac gggaaccgga atgggcatcg ataggttgat gatggcgttg acagggttgt ctattaggga gaccgttttg ttcccgattg ttcggcgtca cagcaactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for MysmA.00612.a.B1.PW38256
PURIFICATION DATe: 4/24/2017
CONCENTRATION: 24mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV RSRVHLSWDF VTPADRDDTS QPQADIPEQF RIRQEKRERL LAQGHDPYPV KVDRTHTLAE LRRAHPDLAA DTATGQIVGV SGRVVFARNS GKLCFATLQE GDGTQLQVMI SLAQVGQEAL DSWKSDVDLG DIVFVHGEVI SSKRGELSVL ADRWQMAAKA LRPLPVAHKE MSEEARVRQR YVDLIVRPEA RTIARQRVAV VRAVRSALER RGFLEVETPM LQTLAGGAAA RPFITHSNAL DIDLYLRIAP ELFLKRCLVG GFEKVFELNR NFRNEGVDST HSPEFAMXET YQAYGDYNDS ATVTRELIQE VADEAIGTRQ VPLPDGTVYD LDGEWDTVEM YPSLSEALGD EITPETPVEK LWQIADRLEV EIPRDRGYGH GKLIEELWEH TVGDKLWAPT FVRDFPVETT PLTRAHRSIP GVTEKWDLYV RKFELATGYS ELIDPVIQRE RFEAQARAAA AGDDEAMVLD EDFLAALEFA MPPTTGTGMG IDRLMMALTG LSIRETVLF
Validated NT Sequence
gggaacaaaa cggtctccct aatagacaac cctgtcaacg ccatcatcaa cctatcgatg cccattccgg ttcccgtggt cggcggcatc gcgaactcca atgcggccag gaaatcctcg tcgagaacca ttgcctcgtc gtcgccggcg gcggcggcgc gggcctgggc ctcgaaacgc tcgcgctgga ttaccgggtc gatgagttcg gagtatcccg tggcgagttc gaatttcctc acatacaggt cccacttctc ggtgacccct ggaatgctgc gatgcgctcg ggtgagtgga gtcgtctcga cggggaagtc gcgcacgaac gtcggggccc acaacttgtc accgaccgtg tgttcccata gttcttcgat caatttcccg tgaccgtaac cgcggtcacg cggaatctcg acctcaagtc gatccgcaat ctgccacaac ttctcgaccg gggtctcggg agtgatctcg tcaccgagtg cctccgacag cgatgggtac atttccaccg tgtcccattc accgtcgaga tcgtagaccg tgccatcggg caatggcacc tgacgcgtgc cgatcgcttc gtcggcgacc tcttgaataa gttctcgggt gacggtcgcg gaatcgttgt agtcaccgta agcctgatat gtctccnnca tcgcgaattc cggcgagtgt gtggaatcca caccctcatt gcgaaagttg cgattcaact cgaaaacctt ctcaaatccg cccacgaggc agcgcttgag gaacagttcc ggcgcgatcc tcaggtacag atcgatgtcg agcgcattgg agtgcgtgat gaacgggcgc gcggcggcac cgccggcgag cgtctgcagc atcggggtct cgacctcgag gaatccgcgc cgttcgagcg ccgaacgcac agcgcggacc accgcgacgc gctggcgggc gatggtgcgg gcctcgggcc ggacgatgag gtcgacatag cgctgacgca cgcgcgcttc ttcgctcatt tctttgtgag caaccggaag tggccgcagc gcctttgccg ccatctgcca tctgtcggcc agcaccgaca attcgccacg cttggaactg atgacttcgc cgtggacgaa cacgatgtca ccgaggtcca cgtcactctt ccaggagtcg agtgcctcct ggcccacctg agcgaggctg atcatcacct gcagttgggt gccgtcgccc tcctgcaggg tcgcgaagca caacttgccc gagttgcggg caaacaccac gcggccggag accccgacga tctggccggt cgcggtgtcg gcggccaggt cggggtgggc gcggcgcagt tcggccagcg tgtgggtgcg atcgaccttc accgggtacg gatcgtgccc ctgcgccagc agccgctccc gcttctcctg acggattcgg aactgctcgg ggatatcggc ctggggctgg gacgtgtcgt cacgatcggc tggggtcacg aaatcccagc ttaaatgaac gcgtgaacgc accatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMR SRVHLSWDFV TPADRDDTSQ PQADIPEQFR IRQEKRERLL AQGHDPYPVK VDRTHTLAEL RRAHPDLAAD TATGQIVGVS GRVVFARNSG KLCFATLQEG DGTQLQVMIS LAQVGQEALD SWKSDVDLGD IVFVHGEVIS SKRGELSVLA DRWQMAAKAL RPLPVAHKEM SEEARVRQRY VDLIVRPEAR TIARQRVAVV RAVRSALERR GFLEVETPML QTLAGGAAAR PFITHSNALD IDLYLRIAPE LFLKRCLVGG FEKVFELNRN FRNEGVDSTH SPEFAMLETY QAYGDYNDSA TVTRELIQEV ADEAIGTRQV PLPDGTVYDL DGEWDTVEMY PSLSEALGDE ITPETPVEKL WQIADRLEVE IPRDRGYGHG KLIEELWEHT VGDKLWAPTF VRDFPVETTP LTRAHRSIPG VTEKWDLYVR KFELATGYSE LIDPVIQRER FEAQARAAAA GDDEAMVLDE DFLAALEFAM PPTTGTGMGI DRLMMALTGL SIRETVLFPI VRRHSN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCGTT CACGCGTTCA TTTAAGCTGG GATTTCGTGA CCCCAGCCGA TCGTGACGAC ACGTCCCAGC CCCAGGCCGA TATCCCCGAG CAGTTCCGAA TCCGTCAGGA GAAGCGGGAG CGGCTGCTGG CGCAGGGGCA CGATCCGTAC CCGGTGAAGG TCGATCGCAC CCACACGCTG GCCGAACTGC GCCGCGCCCA CCCCGACCTG GCCGCCGACA CCGCGACCGG CCAGATCGTC GGGGTCTCCG GCCGCGTGGT GTTTGCCCGC AACTCGGGCA AGTTGTGCTT CGCGACCCTG CAGGAGGGCG ACGGCACCCA ACTGCAGGTG ATGATCAGCC TCGCTCAGGT GGGCCAGGAG GCACTCGACT CCTGGAAGAG TGACGTGGAC CTCGGTGACA TCGTGTTCGT CCACGGCGAA GTCATCAGTT CCAAGCGTGG CGAATTGTCG GTGCTGGCCG ACAGATGGCA GATGGCGGCA AAGGCGCTGC GGCCACTTCC GGTTGCTCAC AAAGAAATGA GCGAAGAAGC GCGCGTGCGT CAGCGCTATG TCGACCTCAT CGTCCGGCCC GAGGCCCGCA CCATCGCCCG CCAGCGCGTC GCGGTGGTCC GCGCTGTGCG TTCGGCGCTC GAACGGCGCG GATTCCTCGA GGTCGAGACC CCGATGCTGC AGACGCTCGC CGGCGGTGCC GCCGCGCGCC CGTTCATCAC GCACTCCAAT GCGCTCGACA TCGATCTGTA CCTGAGGATC GCGCCGGAAC TGTTCCTCAA GCGCTGCCTC GTGGGCGGAT TTGAGAAGGT TTTCGAGTTG AATCGCAACT TTCGCAATGA GGGTGTGGAT TCCACACACT CGCCGGAATT CGCGATGTTG GAGACATATC AGGCTTACGG TGACTACAAC GATTCCGCGA CCGTCACCCG AGAACTTATT CAAGAGGTCG CCGACGAAGC GATCGGCACG CGTCAGGTGC CATTGCCCGA TGGCACGGTC TACGATCTCG ACGGTGAATG GGACACGGTG GAAATGTACC CATCGCTGTC GGAGGCACTC GGTGACGAGA TCACTCCCGA GACCCCGGTC GAGAAGTTGT GGCAGATTGC GGATCGACTT GAGGTCGAGA TTCCGCGTGA CCGCGGTTAC GGTCACGGGA AATTGATCGA AGAACTATGG GAACACACGG TCGGTGACAA GTTGTGGGCC CCGACGTTCG TGCGCGACTT CCCCGTCGAG ACGACTCCAC TCACCCGAGC GCATCGCAGC ATTCCAGGGG TCACCGAGAA GTGGGACCTG TATGTGAGGA AATTCGAACT CGCCACGGGA TACTCCGAAC TCATCGACCC GGTAATCCAG CGCGAGCGTT TCGAGGCCCA GGCCCGCGCC GCCGCCGCCG GCGACGACGA GGCAATGGTT CTCGACGAGG ATTTCCTGGC CGCATTGGAG TTCGCGATGC CGCCGACCAC GGGAACCGGA ATGGGCATCG ATAGGTTGAT GATGGCGTTG ACAGGGTTGT CTATTAGGGA GACCGTTTTG TTCCCGATTG TTCGGCGTCA CAGCAACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT