MysmA.00587.d

Two component response transcriptional regulatory protein prra

CENTER ID: MysmA.00587.d
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00587.d.A1.GE28661 Full length( MysmA.00587.d ) 1 246
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00587.d.A1.PW28560 Full length( MysmA.00587.d ) 1 246

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.240
RefSeq: YP_884659.1
UniProt: A0QP21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVDMTPASG EGSRAPEGVN RTVLMVDDDP DVRTSVARGL RHSGFDVRVA ASGKEALRLL STESHDALVL DVQMPELDGV AVVTALRALG NDIPICVLSA RDTVNDRIAG LEAGADDYLT KPFDLGELVA RLHALLRRAS HTEQPSDTMT VGPVTIDTAR RLVFVSGERV DLTKREFDLL AVLAENAGVV LSRQRLLELV WGYDFDVDTN VADVFISYLR RKLERDGVPR VIHTVRGIGY VLREEP
NT Sequence
GTGCGGGTGG ACATGACCCC CGCCAGCGGT GAGGGATCGC GGGCGCCCGA GGGCGTCAAT CGCACGGTCC TCATGGTCGA CGACGACCCG GATGTCCGAA CCTCGGTGGC GCGCGGCCTG CGCCACTCGG GGTTCGACGT CCGGGTCGCG GCGTCCGGCA AGGAAGCGCT GCGGCTGCTG TCGACGGAGT CGCACGATGC CCTCGTACTG GACGTGCAGA TGCCGGAGCT CGACGGCGTC GCCGTGGTCA CCGCGTTGCG GGCGCTGGGC AACGACATCC CCATCTGTGT GCTGTCGGCA CGCGACACCG TCAACGACCG GATCGCCGGT TTGGAGGCAG GTGCCGATGA CTACCTGACC AAACCGTTCG ACCTCGGTGA ACTGGTGGCC CGGCTGCATG CGCTGCTGCG GCGCGCGAGC CACACCGAGC AGCCGTCCGA CACCATGACA GTCGGACCGG TCACCATCGA CACCGCGCGC AGGCTGGTGT TCGTCAGCGG TGAACGCGTC GACCTGACCA AGCGCGAGTT CGACCTGCTG GCGGTGCTCG CCGAGAACGC GGGCGTGGTG CTGTCCCGGC AGCGGTTGCT GGAGCTGGTG TGGGGCTACG ACTTCGACGT CGACACCAAC GTGGCCGACG TCTTCATCTC CTATCTGCGG CGCAAACTCG AACGCGACGG CGTGCCGCGT GTGATCCACA CCGTGCGCGG CATCGGGTAC GTGCTGCGGG AAGAGCCG
Details for MysmA.00587.d.A1.GE28661
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 29kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVDMTPA SGEGSRAPEG VNRTVLMVDD DPDVRTSVAR GLRHSGFDVR VAASGKEALR LLSTESHDAL VLDVQMPELD GVAVVTALRA LGNDIPICVL SARDTVNDRI AGLEAGADDY LTKPFDLGEL VARLHALLRR ASHTEQPSDT MTVGPVTIDT ARRLVFVSGE RVDLTKREFD LLAVLAENAG VVLSRQRLLE LVWGYDFDVD TNVADVFISY LRRKLERDGV PRVIHTVRGI GYVLRE
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgggtg gacatgaccc ccgccagcgg tgagggatcg cgggcgcccg agggcgtcaa tcgcacggtc ctcatggtcg acgacgaccc ggatgtccga acctcggtgg cgcgcggcct gcgccactcg gggttcgacg tccgggtcgc ggcgtccggc aaggaagcgc tgcggctgct gtcgacggag tcgcacgatg ccctcgtact ggacgtgcag atgccggagc tcgacggcgt cgccgtggtc accgcgttgc gggcgctggg caacgacatc cccatctgtg tgctgtcggc acgcgacacc gtcaacgacc ggatcgccgg tttggaggca ggtgccgatg actacctgac caaaccgttc gacctcggtg aactggtggc ccggctgcat gcgctgctgc ggcgcgcgag ccacaccgag cagccgtccg acaccatgac agtcggaccg gtcaccatcg acaccgcgcg caggctggtg ttcgtcagcg gtgaacgcgt cgacctgacc aagcgcgagt tcgacctgct ggcggtgctc gccgagaacg cgggcgtggt gctgtcccgg cagcggttgc tggagctggt gtggggctac gacttcgacg tcgacaccaa cgtggccgac gtcttcatct cctatctgcg gcgcaaactc gaacgcgacg gcgtgccgcg tgtgatccac accgtgcgcg gcatcgggta cgtgctgcgg gaag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVDMTPA SGEGSRAPEG VNRTVLMVDD DPDVRTSVAR GLRHSGFDVR VAASGKEALR LLSTESHDAL VLDVQMPELD GVAVVTALRA LGNDIPICVL SARDTVNDRI AGLEAGADDY LTKPFDLGEL VARLHALLRR ASHTEQPSDT MTVGPVTIDT ARRLVFVSGE RVDLTKREFD LLAVLAENAG VVLSRQRLLE LVWGYDFDVD TNVADVFISY LRRKLERDGV PRVIHTVRGI GYVLREEP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgggtg gacatgaccc ccgccagcgg tgagggatcg cgggcgcccg agggcgtcaa tcgcacggtc ctcatggtcg acgacgaccc ggatgtccga acctcggtgg cgcgcggcct gcgccactcg gggttcgacg tccgggtcgc ggcgtccggc aaggaagcgc tgcggctgct gtcgacggag tcgcacgatg ccctcgtact ggacgtgcag atgccggagc tcgacggcgt cgccgtggtc accgcgttgc gggcgctggg caacgacatc cccatctgtg tgctgtcggc acgcgacacc gtcaacgacc ggatcgccgg tttggaggca ggtgccgatg actacctgac caaaccgttc gacctcggtg aactggtggc ccggctgcat gcgctgctgc ggcgcgcgag ccacaccgag cagccgtccg acaccatgac agtcggaccg gtcaccatcg acaccgcgcg caggctggtg ttcgtcagcg gtgaacgcgt cgacctgacc aagcgcgagt tcgacctgct ggcggtgctc gccgagaacg cgggcgtggt gctgtcccgg cagcggttgc tggagctggt gtggggctac gacttcgacg tcgacaccaa cgtggccgac gtcttcatct cctatctgcg gcgcaaactc gaacgcgacg gcgtgccgcg tgtgatccac accgtgcgcg gcatcgggta cgtgctgcgg gaagagccga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MysmA.00587.d.A1.PW28560
PURIFICATION DATe: 5/3/2010
CONCENTRATION: 23.61mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVDMTPA SGEGSRAPEG VNRTVLMVDD DPDVRTSVAR GLRHSGFDVR VAASGKEALR LLSTESHDAL VLDVQMPELD GVAVVTALRA LGNDIPICVL SARDTVNDRI AGLEAGADDY LTKPFDLGEL VARLHALLRR ASHTEQPSDT MTVGPVTIDT ARRLVFVSGE RVDLTKREFD LLAVLAENAG VVLSRQRLLE LVWGYDFDVD TNVADVFISY LRRKLERDGV PRVIHTVRGI GYVLRE
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcgggtg gacatgaccc ccgccagcgg tgagggatcg cgggcgcccg agggcgtcaa tcgcacggtc ctcatggtcg acgacgaccc ggatgtccga acctcggtgg cgcgcggcct gcgccactcg gggttcgacg tccgggtcgc ggcgtccggc aaggaagcgc tgcggctgct gtcgacggag tcgcacgatg ccctcgtact ggacgtgcag atgccggagc tcgacggcgt cgccgtggtc accgcgttgc gggcgctggg caacgacatc cccatctgtg tgctgtcggc acgcgacacc gtcaacgacc ggatcgccgg tttggaggca ggtgccgatg actacctgac caaaccgttc gacctcggtg aactggtggc ccggctgcat gcgctgctgc ggcgcgcgag ccacaccgag cagccgtccg acaccatgac agtcggaccg gtcaccatcg acaccgcgcg caggctggtg ttcgtcagcg gtgaacgcgt cgacctgacc aagcgcgagt tcgacctgct ggcggtgctc gccgagaacg cgggcgtggt gctgtcccgg cagcggttgc tggagctggt gtggggctac gacttcgacg tcgacaccaa cgtggccgac gtcttcatct cctatctgcg gcgcaaactc gaacgcgacg gcgtgccgcg tgtgatccac accgtgcgcg gcatcgggta cgtgctgcgg gaag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVRVDMTPA SGEGSRAPEG VNRTVLMVDD DPDVRTSVAR GLRHSGFDVR VAASGKEALR LLSTESHDAL VLDVQMPELD GVAVVTALRA LGNDIPICVL SARDTVNDRI AGLEAGADDY LTKPFDLGEL VARLHALLRR ASHTEQPSDT MTVGPVTIDT ARRLVFVSGE RVDLTKREFD LLAVLAENAG VVLSRQRLLE LVWGYDFDVD TNVADVFISY LRRKLERDGV PRVIHTVRGI GYVLREEP
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCGGGTG GACATGACCC CCGCCAGCGG TGAGGGATCG CGGGCGCCCG AGGGCGTCAA TCGCACGGTC CTCATGGTCG ACGACGACCC GGATGTCCGA ACCTCGGTGG CGCGCGGCCT GCGCCACTCG GGGTTCGACG TCCGGGTCGC GGCGTCCGGC AAGGAAGCGC TGCGGCTGCT GTCGACGGAG TCGCACGATG CCCTCGTACT GGACGTGCAG ATGCCGGAGC TCGACGGCGT CGCCGTGGTC ACCGCGTTGC GGGCGCTGGG CAACGACATC CCCATCTGTG TGCTGTCGGC ACGCGACACC GTCAACGACC GGATCGCCGG TTTGGAGGCA GGTGCCGATG ACTACCTGAC CAAACCGTTC GACCTCGGTG AACTGGTGGC CCGGCTGCAT GCGCTGCTGC GGCGCGCGAG CCACACCGAG CAGCCGTCCG ACACCATGAC AGTCGGACCG GTCACCATCG ACACCGCGCG CAGGCTGGTG TTCGTCAGCG GTGAACGCGT CGACCTGACC AAGCGCGAGT TCGACCTGCT GGCGGTGCTC GCCGAGAACG CGGGCGTGGT GCTGTCCCGG CAGCGGTTGC TGGAGCTGGT GTGGGGCTAC GACTTCGACG TCGACACCAA CGTGGCCGAC GTCTTCATCT CCTATCTGCG GCGCAAACTC GAACGCGACG GCGTGCCGCG TGTGATCCAC ACCGTGCGCG GCATCGGGTA CGTGCTGCGG GAAGAGCCGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A