MysmA.00572.a

ABC transporter ATP-binding protein

CENTER ID: MysmA.00572.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00572.a.A1.GE28657 Full length( MysmA.00572.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.4953
RefSeq: YP_889325.1
UniProt: A0R2D7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRSSVAVEI HGLTKSFGRT RALDGLDMTV PYGHIVGFLG PNGAGKSTTI RVLLGLLRAE RGTVRLLGGD PWHDAVELHR RIAYVPGDVM LWPNLTGMQA IDFLCGLRGD GVDVRRRNQL IDRFELDPHK KARTYSKGNR QKVAIIAAFS CEADLYILDE PTSGLDPLME QAFQQCVTEV AGRGAAVLLS SHILAEVEKL CDDVTIIRSG RTVRSGTLAE LRHLMRTTVT VRTHSDGRVL RDVPYVHDFD CRDGQLRFSV DRTDLEFVME HLIDLGIDDL TVTPASLEDM FLREYQGAVP
NT Sequence
ATGACACGCT CATCGGTCGC GGTCGAGATC CACGGACTGA CAAAGTCTTT CGGGCGCACC AGGGCGCTCG ACGGTCTCGA CATGACAGTG CCGTACGGCC ACATCGTGGG TTTTCTCGGC CCCAACGGCG CGGGGAAGTC CACCACGATC CGCGTGCTGC TGGGACTGTT GCGTGCCGAA CGCGGCACGG TGCGCCTGCT CGGCGGCGAC CCTTGGCACG ACGCCGTGGA ACTGCACCGC CGCATCGCCT ACGTACCGGG CGATGTCATG TTGTGGCCCA ACCTCACCGG GATGCAGGCC ATCGATTTCC TGTGCGGCCT GCGCGGCGAC GGCGTCGATG TGCGCAGACG CAATCAGTTG ATCGACCGGT TCGAACTGGA TCCCCACAAA AAGGCGCGCA CGTACTCGAA GGGCAACCGG CAGAAGGTCG CGATCATCGC GGCGTTCTCG TGCGAGGCCG ATCTCTACAT CCTCGACGAG CCGACCTCAG GCCTGGACCC CTTGATGGAA CAGGCCTTTC AGCAGTGCGT CACCGAGGTG GCCGGGCGCG GCGCCGCGGT GCTGCTGTCG AGTCACATCC TCGCCGAGGT CGAGAAACTG TGTGACGACG TGACGATCAT CCGCTCGGGG CGCACCGTGC GTTCCGGGAC ACTGGCCGAA CTGCGGCACC TGATGCGCAC CACGGTGACG GTGCGCACAC ACTCGGACGG CCGCGTTCTG CGCGATGTGC CCTATGTCCA CGACTTCGAT TGCCGCGACG GGCAATTGCG CTTCTCGGTG GACCGCACCG ACCTGGAGTT CGTCATGGAA CACCTGATCG ACCTGGGTAT CGACGACCTG ACCGTCACGC CCGCCTCGCT GGAGGACATG TTCCTGCGGG AGTATCAGGG AGCCGTCCCG
Details for MysmA.00572.a.A1.GE28657
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 35kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTRSSVAVE IHGLTKSFGR TRALDGLDMT VPYGHIVGFL GPNGAGKSTT IRVLLGLLRA ERGTVRLLGG DPWHDAVELH RRIAYVPGDV MLWPNLTGMQ AIDFLCGLRG DGVDVRRRNQ LIDRFELDPH KKARTYSKGN RQKVAIIAAF SCEADLYILD EPTSGLDPLM EQAFQQCVTE VAGRGAAVLL SSHILAEVEK LCDDVTIIRS GRTVRSGTLA ELRHLMRTTV TVRTHSDGRV LRDVPYVHDF DCRDGQLRFS VDRTDLEFVM EHLIDLGIDD LTVTPASLED MFLREYQGAV P
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacac gctcatcggt cgcggtcgag atccacggac tgacaaagtc tttcgggcgc accagggcgc tcgacggtct cgacatgaca gtgccgtacg gccacatcgt gggttttctc ggccccaacg gcgcggggaa gtccaccacg atccgcgtgc tgctgggact gttgcgtgcc gaacgcggca cggtgcgcct gctcggcggc gacccttggc acgacgccgt ggaactgcac cgccgcatcg cctacgtacc gggcgatgtc atgttgtggc ccaacctcac cgggatgcag gccatcgatt tcctgtgcgg cctgcgcggc gacggcgtcg atgtgcgcag acgcaatcag ttgatcgacc ggttcgaact ggatccccac aaaaaggcgc gcacgtactc gaagggcaac cggcagaagg tcgcgatcat cgcggcgttc tcgtgcgagg ccgatctcta catcctcgac gagccgacct caggcctgga ccccttgatg gaacaggcct ttcagcagtg cgtcaccgag gtggccgggc gcggcgccgc ggtgctgctg tcgagtcaca tcctcgccga ggtcgagaaa ctgtgtgacg acgtgacgat catccgctcg gggcgcaccg tgcgttccgg gacactggcc gaactgcggc acctgatgcg caccacggtg acggtgcgca cacactcgga cggccgcgtt ctgcgcgatg tgccctatgt ccacgacttc gattgccgcg acgggcaatt gcgcttctcg gtggaccgca ccgacctgga gttcgtcatg gaacacctga tcgacctggg tatcgacgac ctgaccgtca cgcccgcctc gctggaggac atgttcctgc gggagtatca gggagccgtc ccgtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTRSSVAVE IHGLTKSFGR TRALDGLDMT VPYGHIVGFL GPNGAGKSTT IRVLLGLLRA ERGTVRLLGG DPWHDAVELH RRIAYVPGDV MLWPNLTGMQ AIDFLCGLRG DGVDVRRRNQ LIDRFELDPH KKARTYSKGN RQKVAIIAAF SCEADLYILD EPTSGLDPLM EQAFQQCVTE VAGRGAAVLL SSHILAEVEK LCDDVTIIRS GRTVRSGTLA ELRHLMRTTV TVRTHSDGRV LRDVPYVHDF DCRDGQLRFS VDRTDLEFVM EHLIDLGIDD LTVTPASLED MFLREYQGAV P
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacgctca tcggtcgcgg tcgagatcca cggactgaca aagtctttcg ggcgcaccag ggcgctcgac ggtctcgaca tgacagtgcc gtacggccac atcgtgggtt ttctcggccc caacggcgcg gggaagtcca ccacgatccg cgtgctgctg ggactgttgc gtgccgaacg cggcacggtg cgcctgctcg gcggcgaccc ttggcacgac gccgtggaac tgcaccgccg catcgcctac gtaccgggcg atgtcatgtt gtggcccaac ctcaccggga tgcaggccat cgatttcctg tgcggcctgc gcggcgacgg cgtcgatgtg cgcagacgca atcagttgat cgaccggttc gaactggatc cccacaaaaa ggcgcgcacg tactcgaagg gcaaccggca gaaggtcgcg atcatcgcgg cgttctcgtg cgaggccgat ctctacatcc tcgacgagcc gacctcaggc ctggacccct tgatggaaca ggcctttcag cagtgcgtca ccgaggtggc cgggcgcggc gccgcggtgc tgctgtcgag tcacatcctc gccgaggtcg agaaactgtg tgacgacgtg acgatcatcc gctcggggcg caccgtgcgt tccgggacac tggccgaact gcggcacctg atgcgcacca cggtgacggt gcgcacacac tcggacggcc gcgttctgcg cgatgtgccc tatgtccacg acttcgattg ccgcgacggg caattgcgct tctcggtgga ccgcaccgac ctggagttcg tcatggaaca cctgatcgac ctgggtatcg acgacctgac cgtcacgccc gcctcgctgg aggacatgtt cctgcgggag tatcagggag ccgtcccgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa