MysmA.00560.a
Proline imino-peptidase
| CENTER ID: | MysmA.00560.a |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00560.a.A1.GE28656 | Full length( MysmA.00560.a ) | 1 | 309 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00560.a.A1.PW28555 | Full length( MysmA.00560.a ) | 1 | 309 |
Structures
3NWO
DEPOSITED: 7/9/2010
DETERMINATION: XRay
CLONE: MysmA.00560.a.A1.GE28656
PROTEIN: MysmA.00560.a.A1.PW28555
Publications by SSGCID
Increasing the structural coverage of tuberculosis drug targets.
Abendroth J, Abramov A, Armour B, Barrett LK, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Ferrell M, Fox D, Gardberg AS, Hewitt SN, Lorimer D, Lyons-Abbott S, Mundt E, Muruthi MM, Myers J, Myler PJ, Napuli AJ, Phan I, Sekar A, Serbzhinskiy D, Stacy R, Staker BL, Stewart LJ, Taylor BM, Thompkins K, Tran N, Van Voorhis WC, Zhang Y
Tuberculosis (Edinb) - 2014
volume 95, issue 2, pages 142-8
PMID: 25613812; PMCID: PMC4361283
PDB:
3GVC,
3GVG,
3GWC,
3H7F,
3H81,
3HE2,
3HWI,
3HWK,
3HZG,
3ICO,
3KHP,
3LLS,
3MOY,
3MPZ,
3MYB,
3NDN,
3NDO,
3NF4,
3NG3,
3NJD,
3NWO,
3O0M,
3O38,
3OC6,
3OC7,
3OEC,
3OI9,
3OKS,
3OL3,
3OL4,
3OME,
3P0T,
3P2Y,
3P4I,
3P4T,
3P5M,
3P85,
3PE8,
3PGX,
3PK0,
3PPI,
3PXX,
3PZY,
3Q1T,
3Q8N,
3QBP,
3QDF,
3QH4,
3QHA,
3QIV,
3QK8,
3QKA,
3QLJ,
3QMJ,
3QRE,
3QUA,
3QUV,
3QXI,
3QXZ,
3QYR,
3R0O,
3R1I,
3R1J,
3R20,
3R2N,
3R4T,
3R6H,
3R6O,
3R7K,
3R8C,
3R9P,
3R9Q,
3R9R,
3R9S,
3R9T,
3RD5,
3RD7,
3RD8,
3RFQ,
3RIH,
3RR2,
3RR6,
3RRP,
3RRV,
3RSI,
3RV2,
3S55,
3S82,
3SBX,
3SF6,
3SLL,
3SVK,
3SVT,
3SWO,
3SWT,
3SWX,
3SX2,
3T3W,
3T7C,
3TAV,
3TCR,
3TDE,
3TJR,
3TL3,
3TLF,
3TRR,
3TSC,
3TX2,
3TZQ,
3TZU,
3U0A,
3U0B,
3UCX,
3UVE,
4DI1,
4DIE,
4DQ8,
4DXL,
4ED4,
4EGE,
4EGF,
4EMD,
4EO9,
4EYE,
4F3W,
4F47,
4FFC,
4GK6,
4HDT,
4HR3,
4I1Y,
4IJN,
4IV6,
4IZ9,
4J5I,
4KAM,
4LGV,
4O2D,
5EJ2
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_144415 |
| PATRIC ID: | fig|246196.19.peg.2648 |
| RefSeq: | YP_887016.1 |
| UniProt: | A0QVS8 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLSRMPVSSR TVPFGDHETW VQVTTPENAQ PHALPLIVLH GGPGMAHNYV ANIAALADET GRTVIHYDQV GCGNSTHLPD APADFWTPQL FVDEFHAVCT ALGIERYHVL GQSWGGMLGA EIAVRQPSGL VSLAICNSPA SMRLWSEAAG DLRAQLPAET RAALDRHEAA GTITHPDYLQ AAAEFYRRHV CRVVPTPQDF ADSVAQMEAE PTVYHTMNGP NEFHVVGTLG DWSVIDRLPD VTAPVLVIAG EHDEATPKTW QPFVDHIPDV RSHVFPGTSH CTHLEKPEEF RAVVAQFLHQ HDLAADARV
NT Sequence
ATGCTGTCCC GCATGCCTGT TTCGTCCCGC ACCGTGCCGT TCGGCGATCA CGAGACCTGG GTCCAGGTCA CGACGCCGGA GAATGCCCAG CCCCACGCGC TTCCCCTGAT CGTGCTGCAC GGCGGGCCCG GCATGGCCCA CAACTACGTC GCCAACATCG CCGCCCTCGC CGACGAGACC GGCCGCACCG TCATCCACTA CGACCAGGTG GGCTGCGGAA ACAGCACGCA CCTTCCTGAC GCACCCGCGG ACTTCTGGAC ACCGCAACTG TTCGTCGACG AATTCCACGC CGTGTGCACC GCGCTGGGCA TCGAGCGCTA CCACGTGCTC GGGCAGTCGT GGGGCGGCAT GCTGGGCGCC GAGATCGCGG TGCGCCAACC GTCCGGTCTC GTCTCGCTCG CCATCTGCAA CTCGCCGGCG TCGATGAGGT TGTGGTCGGA GGCCGCCGGC GACCTGCGGG CACAGTTACC CGCCGAGACG CGCGCCGCGC TCGATCGCCA CGAGGCCGCA GGCACCATCA CCCACCCTGA CTATCTGCAG GCCGCGGCCG AGTTCTACCG ACGCCACGTG TGCCGCGTCG TGCCGACGCC ACAGGACTTC GCCGACAGCG TCGCCCAGAT GGAAGCCGAG CCGACGGTGT ACCACACCAT GAACGGCCCC AACGAGTTTC ATGTCGTCGG CACCCTCGGC GACTGGAGCG TCATCGACCG GCTGCCCGAC GTCACCGCGC CGGTCCTCGT GATCGCGGGT GAACACGACG AGGCGACACC CAAGACGTGG CAGCCGTTCG TCGACCACAT CCCCGACGTG CGCAGCCACG TGTTCCCCGG CACCAGCCAT TGCACGCATC TGGAGAAGCC CGAGGAATTC CGCGCCGTCG TCGCGCAGTT CCTCCATCAA CACGATCTGG CCGCCGACGC CCGGGTC
Details for MysmA.00560.a.A1.GE28656
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 37kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSRMPVSS RTVPFGDHET WVQVTTPENA QPHALPLIVL HGGPGMAHNY VANIAALADE TGRTVIHYDQ VGCGNSTHLP DAPADFWTPQ LFVDEFHAVC TALGIERYHV LGQSWGGMLG AEIAVRQPSG LVSLAICNSP ASMRLWSEAA GDLRAQLPAE TRAALDRHEA AGTITHPDYL QAAAEFYRRH VCRVVPTPQD FADSVAQMEA EPTVYHTMNG PNEFHVVGTL GDWSVIDRLP DVTAPVLVIA GEHDEATPKT WQPFVDHIPD VRSXVFPGTS HCTHLEKPEE XRAVXXXXLH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgt cccgcatgcc tgtttcgtcc cgcaccgtgc cgttcggcga tcacgagacc tgggtccagg tcacgacgcc ggagaatgcc cagccccacg cgcttcccct gatcgtgctg cacggcgggc ccggcatggc ccacaactac gtcgccaaca tcgccgccct cgccgacgag accggccgca ccgtcatcca ctacgaccag gtgggctgcg gaaacagcac gcaccttcct gacgcacccg cggacttctg gacaccgcaa ctgttcgtcg acgaattcca cgccgtgtgc accgcgctgg gcatcgagcg ctaccacgtg ctcgggcagt cgtggggcgg catgctgggc gccgagatcg cggtgcgcca accgtccggt ctcgtctcgc tcgccatctg caactcgccg gcgtcgatga ggttgtggtc ggaggccgcc ggcgacctgc gggcacagtt acccgccgag acgcgcgccg cgctcgatcg ccacgaggcc gcaggcacca tcacccaccc tgactatctg caggccgcgg ccgagttcta ccgacgccac gtgtgccgcg tcgtgccgac gccacaggac ttcgccgaca gcgtcgccca gatggaagcc gagccgacgg tgtaccacac catgaacggc cccaacgagt ttcatgtcgt cggcaccctc ggcgactgga gcgtcatcga ccggctgccc gacgtcaccg cgccggtcct cgtgatcgcg ggtgaacacg acgaggcgac acccaagacg tggcagccgt tcgtcgacca catccccgac gtgcgcagcc ncgtgttccc cggcaccagc cattgcacgc atctggagaa gcccgaggaa ntncgcgccg tcnncncgca ntncctccat na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSRMPVSS RTVPFGDHET WVQVTTPENA QPHALPLIVL HGGPGMAHNY VANIAALADE TGRTVIHYDQ VGCGNSTHLP DAPADFWTPQ LFVDEFHAVC TALGIERYHV LGQSWGGMLG AEIAVRQPSG LVSLAICNSP ASMRLWSEAA GDLRAQLPAE TRAALDRHEA AGTITHPDYL QAAAEFYRRH VCRVVPTPQD FADSVAQMEA EPTVYHTMNG PNEFHVVGTL GDWSVIDRLP DVTAPVLVIA GEHDEATPKT WQPFVDHIPD VRSHVFPGTS HCTHLEKPEE FRAVVAQFLH QHDLAADARV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgtcccgc atgcctgttt cgtcccgcac cgtgccgttc ggcgatcacg agacctgggt ccaggtcacg acgccggaga atgcccagcc ccacgcgctt cccctgatcg tgctgcacgg cgggcccggc atggcccaca actacgtcgc caacatcgcc gccctcgccg acgagaccgg ccgcaccgtc atccactacg accaggtggg ctgcggaaac agcacgcacc ttcctgacgc acccgcggac ttctggacac cgcaactgtt cgtcgacgaa ttccacgccg tgtgcaccgc gctgggcatc gagcgctacc acgtgctcgg gcagtcgtgg ggcggcatgc tgggcgccga gatcgcggtg cgccaaccgt ccggtctcgt ctcgctcgcc atctgcaact cgccggcgtc gatgaggttg tggtcggagg ccgccggcga cctgcgggca cagttacccg ccgagacgcg cgccgcgctc gatcgccacg aggccgcagg caccatcacc caccctgact atctgcaggc cgcggccgag ttctaccgac gccacgtgtg ccgcgtcgtg ccgacgccac aggacttcgc cgacagcgtc gcccagatgg aagccgagcc gacggtgtac cacaccatga acggccccaa cgagtttcat gtcgtcggca ccctcggcga ctggagcgtc atcgaccggc tgcccgacgt caccgcgccg gtcctcgtga tcgcgggtga acacgacgag gcgacaccca agacgtggca gccgttcgtc gaccacatcc ccgacgtgcg cagccacgtg ttccccggca ccagccattg cacgcatctg gagaagcccg aggaattccg cgccgtcgtc gcgcagttcc tccatcaaca cgatctggcc gccgacgccc gggtcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.00560.a.A1.PW28555
| PURIFICATION DATe: | 4/26/2010 |
| CONCENTRATION: | 22.63mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 100µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLSRMPVSS RTVPFGDHET WVQVTTPENA QPHALPLIVL HGGPGMAHNY VANIAALADE TGRTVIHYDQ VGCGNSTHLP DAPADFWTPQ LFVDEFHAVC TALGIERYHV LGQSWGGMLG AEIAVRQPSG LVSLAICNSP ASMRLWSEAA GDLRAQLPAE TRAALDRHEA AGTITHPDYL QAAAEFYRRH VCRVVPTPQD FADSVAQMEA EPTVYHTMNG PNEFHVVGTL GDWSVIDRLP DVTAPVLVIA GEHDEATPKT WQPFVDHIPD VRSXVFPGTS HCTHLEKPEE XRAVXXXXLH
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctgt cccgcatgcc tgtttcgtcc cgcaccgtgc cgttcggcga tcacgagacc tgggtccagg tcacgacgcc ggagaatgcc cagccccacg cgcttcccct gatcgtgctg cacggcgggc ccggcatggc ccacaactac gtcgccaaca tcgccgccct cgccgacgag accggccgca ccgtcatcca ctacgaccag gtgggctgcg gaaacagcac gcaccttcct gacgcacccg cggacttctg gacaccgcaa ctgttcgtcg acgaattcca cgccgtgtgc accgcgctgg gcatcgagcg ctaccacgtg ctcgggcagt cgtggggcgg catgctgggc gccgagatcg cggtgcgcca accgtccggt ctcgtctcgc tcgccatctg caactcgccg gcgtcgatga ggttgtggtc ggaggccgcc ggcgacctgc gggcacagtt acccgccgag acgcgcgccg cgctcgatcg ccacgaggcc gcaggcacca tcacccaccc tgactatctg caggccgcgg ccgagttcta ccgacgccac gtgtgccgcg tcgtgccgac gccacaggac ttcgccgaca gcgtcgccca gatggaagcc gagccgacgg tgtaccacac catgaacggc cccaacgagt ttcatgtcgt cggcaccctc ggcgactgga gcgtcatcga ccggctgccc gacgtcaccg cgccggtcct cgtgatcgcg ggtgaacacg acgaggcgac acccaagacg tggcagccgt tcgtcgacca catccccgac gtgcgcagcc ncgtgttccc cggcaccagc cattgcacgc atctggagaa gcccgaggaa ntncgcgccg tcnncncgca ntncctccat na
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLSRMPVSS RTVPFGDHET WVQVTTPENA QPHALPLIVL HGGPGMAHNY VANIAALADE TGRTVIHYDQ VGCGNSTHLP DAPADFWTPQ LFVDEFHAVC TALGIERYHV LGQSWGGMLG AEIAVRQPSG LVSLAICNSP ASMRLWSEAA GDLRAQLPAE TRAALDRHEA AGTITHPDYL QAAAEFYRRH VCRVVPTPQD FADSVAQMEA EPTVYHTMNG PNEFHVVGTL GDWSVIDRLP DVTAPVLVIA GEHDEATPKT WQPFVDHIPD VRSHVFPGTS HCTHLEKPEE FRAVVAQFLH QHDLAADARV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTGTCCCGC ATGCCTGTTT CGTCCCGCAC CGTGCCGTTC GGCGATCACG AGACCTGGGT CCAGGTCACG ACGCCGGAGA ATGCCCAGCC CCACGCGCTT CCCCTGATCG TGCTGCACGG CGGGCCCGGC ATGGCCCACA ACTACGTCGC CAACATCGCC GCCCTCGCCG ACGAGACCGG CCGCACCGTC ATCCACTACG ACCAGGTGGG CTGCGGAAAC AGCACGCACC TTCCTGACGC ACCCGCGGAC TTCTGGACAC CGCAACTGTT CGTCGACGAA TTCCACGCCG TGTGCACCGC GCTGGGCATC GAGCGCTACC ACGTGCTCGG GCAGTCGTGG GGCGGCATGC TGGGCGCCGA GATCGCGGTG CGCCAACCGT CCGGTCTCGT CTCGCTCGCC ATCTGCAACT CGCCGGCGTC GATGAGGTTG TGGTCGGAGG CCGCCGGCGA CCTGCGGGCA CAGTTACCCG CCGAGACGCG CGCCGCGCTC GATCGCCACG AGGCCGCAGG CACCATCACC CACCCTGACT ATCTGCAGGC CGCGGCCGAG TTCTACCGAC GCCACGTGTG CCGCGTCGTG CCGACGCCAC AGGACTTCGC CGACAGCGTC GCCCAGATGG AAGCCGAGCC GACGGTGTAC CACACCATGA ACGGCCCCAA CGAGTTTCAT GTCGTCGGCA CCCTCGGCGA CTGGAGCGTC ATCGACCGGC TGCCCGACGT CACCGCGCCG GTCCTCGTGA TCGCGGGTGA ACACGACGAG GCGACACCCA AGACGTGGCA GCCGTTCGTC GACCACATCC CCGACGTGCG CAGCCACGTG TTCCCCGGCA CCAGCCATTG CACGCATCTG GAGAAGCCCG AGGAATTCCG CGCCGTCGTC GCGCAGTTCC TCCATCAACA CGATCTGGCC GCCGACGCCC GGGTCAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA