MysmA.00554.a

3-Hydroxyacyl-CoA dehydrogenase

CENTER ID: MysmA.00554.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00554.a.A1.GE28653 Full length( MysmA.00554.a ) 1 253
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5057
RefSeq: YP_889429.1
UniProt: A0R2P1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEIKDAVAVV TGGASGLGLA TTKRLLDAGA QVVVIDLKGE EVVAELGDRA KFVATDVTDE AGVTEALNVA ESLGPVRINV NCAGIGNAIK TLSKNGPFPL DGFRKVVEVN LIGTFNVIRL SAERIAKTEP IGPNGEERGV IINTASVAAF DGQIGQAAYS ASKGGVVGMT LPIARDLSRE LIRVVTIAPG LFKTPLLGSL PEEAQKSLGA QVPHPARLGD PDEYGALAQH IIENPMLNGE VIRLDGAIRM APR
NT Sequence
GTGGAGATCA AAGACGCGGT AGCCGTCGTC ACCGGCGGCG CATCCGGCCT GGGCCTGGCC ACCACCAAGC GGCTGCTCGA CGCGGGCGCG CAGGTCGTGG TGATCGACCT CAAGGGTGAA GAGGTCGTCG CCGAGCTCGG CGATCGCGCC AAGTTCGTCG CGACCGACGT GACCGACGAG GCCGGTGTCA CCGAGGCGCT GAACGTGGCC GAGTCGCTGG GCCCGGTGCG CATCAACGTC AACTGCGCCG GTATCGGCAA CGCGATCAAG ACGCTGAGCA AGAACGGCCC CTTCCCGCTC GACGGGTTCC GCAAGGTCGT CGAGGTCAAC CTGATCGGCA CGTTCAACGT GATCCGCCTG AGCGCGGAGC GCATCGCCAA GACCGAGCCC ATCGGGCCCA ACGGCGAAGA GCGCGGCGTC ATCATCAACA CCGCCTCGGT CGCGGCGTTC GACGGCCAGA TCGGCCAGGC CGCGTACTCG GCGTCCAAGG GCGGCGTGGT CGGCATGACC CTGCCGATCG CGCGCGACCT CTCGCGTGAG CTGATCCGCG TCGTCACCAT CGCGCCGGGT CTGTTCAAGA CGCCGCTTCT GGGCTCGCTG CCCGAGGAGG CGCAGAAGTC CCTCGGCGCC CAGGTGCCGC ATCCGGCGCG GCTGGGTGAT CCCGACGAGT ACGGCGCGCT CGCGCAGCAC ATCATCGAGA ACCCGATGCT CAACGGCGAG GTGATCCGTC TCGACGGCGC CATCCGCATG GCACCGCGA
Details for MysmA.00554.a.A1.GE28653
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVEIKDAVA VVTGGASGLG LATTKRLLDA GAQVVVIDLK GEEVVAELGD RAKFVATDVT DEAGVTEALN VAESLGPVRI NVNCAGIGNA IKTLSKNGPF PLDGFRKVVE VNLIGTFNVI RLSAERIAKT EPIGPNGEER GVIINTASVA AFDGQIGQAA YSASKGGVVG MTLPIARDLS RELIRVVTIA PGLFKTPLLG SLPEEAQKSL GAQVPHPARL GDPDEYGALA QHIIENPMLX GEVIRLDGAX RMAPR
Validated NT Sequence
ttttnnttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgg agatcaaaga cgcggtagcc gtcgtcaccg gcggcgcatc cggcctgggc ctggccacca ccaagcggct gctcgacgcg ggcgcgcagg tcgtggtgat cgacctcaag ggtgaagagg tcgtcgccga gctcggcgat cgcgccaagt tcgtcgcgac cgacgtgacc gacgaggccg gtgtcaccga ggcgctgaac gtggccgagt cgctgggccc ggtgcgcatc aacgtcaact gcgccggtat cggcaacgcg atcaagacgc tgagcaagaa cggccccttc ccgctcgacg ggttccgcaa ggtcgtcgag gtcaacctga tcggcacgtt caacgtgatc cgcctgagcg cggagcgcat cgccaagacc gagcccatcg ggcccaacgg cgaagagcgc ggcgtcatca tcaacaccgc ctcggtcgcg gcgttcgacg gccagatcgg ccaggccgcg tactcggcgt ccaagggcgg cgtggtcggc atgaccctgc cgatcgcgcg cgacctctcg cgtgagctga tccgcgtcgt caccatcgcg ccgggtctgt tcaagacgcc gcttctgggc tcgctgcccg aggaggcgca gaagtccctc ggcgcccagg tgccgcatcc ggcgcggctg ggtgatcccg acgagtacgg cgcgctcgcg cagcacatca tcgagaaccc gatgctcnac ggcgaggtga tccgtctcga cggcgccntc cgcatggcac cgcgataata aacagcacga acaagttctg cagccnagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct ganttggctg ctgccnccgc tgancaatac tagcatnacc ccntggggcn tctaaacggg tnntgnnggg tttttgctga aangaggaac tatntccggn nntcnnnngn ngggngnngn cgncntgntc ncgtanncna tanngnnnca ntannnaann nnncagnact gggcngcnnn nnnngnngnn nnngncnnnn nngnngnnnn nnnnnnnnnt cancnnnnnn nnnggnnnnn nngnnnnnnn nnnannnncn nnnnnnnnnn ngnncnnnnn nnnnnnnnnn nnnannnnnn cnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVEIKDAVA VVTGGASGLG LATTKRLLDA GAQVVVIDLK GEEVVAELGD RAKFVATDVT DEAGVTEALN VAESLGPVRI NVNCAGIGNA IKTLSKNGPF PLDGFRKVVE VNLIGTFNVI RLSAERIAKT EPIGPNGEER GVIINTASVA AFDGQIGQAA YSASKGGVVG MTLPIARDLS RELIRVVTIA PGLFKTPLLG SLPEEAQKSL GAQVPHPARL GDPDEYGALA QHIIENPMLN GEVIRLDGAI RMAPR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggagatc aaagacgcgg tagccgtcgt caccggcggc gcatccggcc tgggcctggc caccaccaag cggctgctcg acgcgggcgc gcaggtcgtg gtgatcgacc tcaagggtga agaggtcgtc gccgagctcg gcgatcgcgc caagttcgtc gcgaccgacg tgaccgacga ggccggtgtc accgaggcgc tgaacgtggc cgagtcgctg ggcccggtgc gcatcaacgt caactgcgcc ggtatcggca acgcgatcaa gacgctgagc aagaacggcc ccttcccgct cgacgggttc cgcaaggtcg tcgaggtcaa cctgatcggc acgttcaacg tgatccgcct gagcgcggag cgcatcgcca agaccgagcc catcgggccc aacggcgaag agcgcggcgt catcatcaac accgcctcgg tcgcggcgtt cgacggccag atcggccagg ccgcgtactc ggcgtccaag ggcggcgtgg tcggcatgac cctgccgatc gcgcgcgacc tctcgcgtga gctgatccgc gtcgtcacca tcgcgccggg tctgttcaag acgccgcttc tgggctcgct gcccgaggag gcgcagaagt ccctcggcgc ccaggtgccg catccggcgc ggctgggtga tcccgacgag tacggcgcgc tcgcgcagca catcatcgag aacccgatgc tcaacggcga ggtgatccgt ctcgacggcg ccatccgcat ggcaccgcga aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa