MysmA.00549.a

Molybdenum cofactor biosynthesis protein A

CENTER ID: MysmA.00549.a
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00549.a.A1.GE28652 Full length( MysmA.00549.a ) 1 361
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.5553
RefSeq: YP_889931.1
UniProt: A0R443

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVTALGLPT VARSTGDGSA GASPAPADGP LVDTYGRAAT DLRVSLTDRC NLRCTYCMPA EGLNWLPGDA LLSPTELARL LRIAVARLGI TSVRFTGGEP LVVRHLEEVV AAAAALEPRP EITLTTNGLG LARRAEGLKK AGLNRINVSL DSVDAAHFAR ITRRDRLPDV LAGLAAAKAA GLEPVKVNAV LDPVSGLDDA VELLRYCLQH GYQLRIIEQM PLDASHSWQR DNVIDADRIL QTLQQHFELT PDSRPRGSAP AELWQVAEGP THAAGTVGVI ASVSHAFCSA CDRTRLTADG QIRSCLFSRE ETDLRHLLRG GADDAEIEAA WRAAMWSKPA GHGINDPDFV QPVRPMSAIG G
NT Sequence
ATGACCGTCA CGGCGCTCGG CCTACCCACC GTCGCCCGGT CCACGGGCGA CGGTTCTGCT GGGGCCTCCC CTGCGCCCGC CGACGGCCCC CTGGTCGACA CATACGGACG TGCCGCGACC GACCTGCGCG TGTCGCTGAC CGACCGCTGC AATCTGCGCT GCACCTATTG CATGCCTGCC GAGGGCCTCA ACTGGCTTCC CGGTGACGCG CTGCTGAGCC CCACCGAACT GGCCCGTCTG CTGCGCATCG CGGTGGCCCG GCTCGGCATC ACCAGCGTGC GGTTCACCGG CGGTGAGCCG TTGGTGGTGC GTCACCTCGA AGAGGTCGTC GCGGCGGCCG CCGCACTTGA GCCCCGCCCG GAGATCACGC TGACCACCAA CGGCCTCGGC CTGGCCCGTC GTGCAGAGGG GCTCAAGAAG GCCGGCCTCA ACCGCATCAA CGTGTCACTG GACAGCGTCG ACGCCGCGCA CTTCGCACGC ATCACCCGCC GCGATCGCCT GCCCGACGTG CTGGCCGGGC TGGCGGCCGC GAAGGCGGCC GGGCTCGAGC CCGTGAAGGT CAACGCGGTC CTCGATCCCG TGTCGGGCCT CGACGACGCC GTGGAGCTGC TGCGGTACTG CCTGCAGCAC GGATATCAGC TGCGGATCAT CGAGCAGATG CCGCTCGACG CGAGCCACAG CTGGCAGCGC GACAACGTCA TCGACGCCGA CCGGATCCTG CAGACCCTGC AACAGCACTT CGAGCTGACC CCGGATTCGC GTCCGCGCGG GTCGGCGCCC GCCGAGCTGT GGCAGGTGGC CGAGGGACCC ACGCACGCCG CGGGCACGGT CGGGGTCATC GCGTCGGTGT CGCATGCCTT CTGTTCGGCC TGTGACCGCA CGCGGCTGAC CGCGGACGGT CAGATCCGCA GCTGCCTGTT CTCGCGCGAG GAGACCGATC TGCGGCACCT GCTGCGCGGC GGTGCCGACG ACGCCGAGAT CGAGGCCGCG TGGCGCGCGG CGATGTGGAG CAAGCCCGCC GGACACGGCA TCAACGATCC TGATTTCGTG CAGCCGGTCC GGCCCATGAG TGCGATCGGT GGA
Details for MysmA.00549.a.A1.GE28652
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMTVTALG LPTVARSTGD GSAGASPAPA DGPLVDTYGR AATDLRVSLT DRCNLRCTYC MPAEGLNWLP GDALLSPTEL ARLLRIAVAR LGITSVRFTG GEPLVVRHLE EVVAAAAALE PRPEITLTTN GLGLARRAEG LKKAGLNRIN VSLDSVDAAH FARITRRDRL PDVLAGLAAA KAAGLEPVKV NAVLDPVSGL DDAVELLRYC LQHGYQLRII EQMPLDASHS WQRDNVIDAD RILQTLQQHF ELTPDSRPRG SAPAELWQVA EGPTHAAGTV GVIASVSHAF CSACDRTRLT ADGQIRSCLF SREETDLRHL LRGGADDAEI EAAWRAAMWS KPAGHGINDP DFVQPVRPMS A
Validated NT Sequence
ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaccgt cacggcgctc ggcctaccca ccgtcgcccg gtccacgggc gacggttctg ctggggcctc ccctgcgccc gccgacggcc ccctggtcga cacatacgga cgtgccgcga ccgacctgcg cgtgtcgctg accgaccgct gcaatctgcg ctgcacctat tgcatgcctg ccgagggcct caactggctt cccggtgacg cgctgctgag ccccaccgaa ctggcccgtc tgctgcgcat cgcggtggcc cggctcggca tcaccagcgt gcggttcacc ggcggtgagc cgttggtggt gcgtcacctc gaagaggtcg tcgcggcggc cgccgcactt gagccccgcc cggagatcac gctgaccacc aacggcctcg gcctggcccg tcgtgcagag gggctcaaga aggccggcct caaccgcatc aacgtgtcac tggacagcgt cgacgccgcg cacttcgcac gcatcacccg ccgcgatcgc ctgcccgacg tgctggccgg gctggcggcc gcgaaggcgg ccgggctcga gcccgtgaag gtcaacgcgg tcctcgatcc cgtgtcgggc ctcgacgacg ccgtggagct gctgcggtac tgcctgcagc acggatatca gctgcggatc atcgagcaga tgccgctcga cgcgagccac agctggcagc gcgacaacgt catcgacgcc gaccggatcc tgcagaccct gcaacagcac ttcgagctga ccccggattc gcgtccgcgc gggtcggcgc ccgccgagct gtggcaggtg gccgagggac ccacgcacgc cgcgggcacg gtcggggtca tcgcgtcggt gtcgcatgcc ttctgttcgg cctgtgaccg cacgcggctg accgcggacg gtcagatccg cagctgcctg ttctcgcgcg aggagaccga tctgcggcac ctgctgcgcg gcggtgccga cgacgccgag atcgaggccg cgtggcgcgc ggcgatgtgg agcaagcccg ccggacacgg catcaacgat cctgatttcg tgcagccggt ccggcccatg agtgcgat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVTALGLP TVARSTGDGS AGASPAPADG PLVDTYGRAA TDLRVSLTDR CNLRCTYCMP AEGLNWLPGD ALLSPTELAR LLRIAVARLG ITSVRFTGGE PLVVRHLEEV VAAAAALEPR PEITLTTNGL GLARRAEGLK KAGLNRINVS LDSVDAAHFA RITRRDRLPD VLAGLAAAKA AGLEPVKVNA VLDPVSGLDD AVELLRYCLQ HGYQLRIIEQ MPLDASHSWQ RDNVIDADRI LQTLQQHFEL TPDSRPRGSA PAELWQVAEG PTHAAGTVGV IASVSHAFCS ACDRTRLTAD GQIRSCLFSR EETDLRHLLR GGADDAEIEA AWRAAMWSKP AGHGINDPDF VQPVRPMSAI GG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgtcacg gcgctcggcc tacccaccgt cgcccggtcc acgggcgacg gttctgctgg ggcctcccct gcgcccgccg acggccccct ggtcgacaca tacggacgtg ccgcgaccga cctgcgcgtg tcgctgaccg accgctgcaa tctgcgctgc acctattgca tgcctgccga gggcctcaac tggcttcccg gtgacgcgct gctgagcccc accgaactgg cccgtctgct gcgcatcgcg gtggcccggc tcggcatcac cagcgtgcgg ttcaccggcg gtgagccgtt ggtggtgcgt cacctcgaag aggtcgtcgc ggcggccgcc gcacttgagc cccgcccgga gatcacgctg accaccaacg gcctcggcct ggcccgtcgt gcagaggggc tcaagaaggc cggcctcaac cgcatcaacg tgtcactgga cagcgtcgac gccgcgcact tcgcacgcat cacccgccgc gatcgcctgc ccgacgtgct ggccgggctg gcggccgcga aggcggccgg gctcgagccc gtgaaggtca acgcggtcct cgatcccgtg tcgggcctcg acgacgccgt ggagctgctg cggtactgcc tgcagcacgg atatcagctg cggatcatcg agcagatgcc gctcgacgcg agccacagct ggcagcgcga caacgtcatc gacgccgacc ggatcctgca gaccctgcaa cagcacttcg agctgacccc ggattcgcgt ccgcgcgggt cggcgcccgc cgagctgtgg caggtggccg agggacccac gcacgccgcg ggcacggtcg gggtcatcgc gtcggtgtcg catgccttct gttcggcctg tgaccgcacg cggctgaccg cggacggtca gatccgcagc tgcctgttct cgcgcgagga gaccgatctg cggcacctgc tgcgcggcgg tgccgacgac gccgagatcg aggccgcgtg gcgcgcggcg atgtggagca agcccgccgg acacggcatc aacgatcctg atttcgtgca gccggtccgg cccatgagtg cgatcggtgg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa