MysmA.00471.c
5-carboxymethyl-2-hydroxymuconate delta-isomerase
| CENTER ID: | MysmA.00471.c |
| ORGANISM: | Mycobacterium smegmatis ATCC 700084 / mc(2)155 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIC |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00471.c.A1.GE28647 | Full length( MysmA.00471.c ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| MysmA.00471.c.A1.PS00549 | Full length( MysmA.00471.c ) | 1 | 259 |
Structures
4PFZ
DEPOSITED: 5/1/2014
DETERMINATION: XRay
CLONE: MysmA.00471.c.A1.GE28647
PROTEIN: MysmA.00471.c.A1.PS00549
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126763 |
| PATRIC ID: | fig|246196.19.peg.2348 |
| RefSeq: | YP_886724.1 |
| UniProt: | A0QUY6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLGRIASPD GVAFVSIEGD GPDAVCKEIA EHPFGNPNFT GRSWPLADVR LLAPILASKV IAVGKNYAAH AEEMGGVAPE DPVIFLKPNT AIIGPNVPIQ LPADADPVHH EGELAIVIGR PCKDVPAARA AEFILGYTIG NDVSARDQQR KDGQWMRAKG HDTFCPLGPW IVTDLDPSDV ELRTEVNGQV RQRSRTSLLL HDIGRLVEWT SAVMTLLPGD VILTGTPEGV GPIEDGDTVS ITVEGIGTLT NPVVRKGKK
NT Sequence
ATGCGCTTAG GTCGAATTGC CAGTCCTGAC GGCGTCGCAT TCGTGAGCAT CGAAGGCGAC GGACCAGATG CCGTCTGCAA AGAGATCGCC GAGCATCCGT TCGGAAACCC GAACTTCACC GGACGCAGCT GGCCGCTGGC CGACGTCCGC CTGCTCGCAC CGATTCTCGC CAGCAAGGTG ATCGCGGTGG GTAAGAACTA CGCCGCGCAC GCCGAGGAGA TGGGCGGTGT GGCGCCCGAG GATCCGGTGA TCTTCCTCAA ACCGAACACC GCGATCATCG GTCCCAACGT GCCGATCCAG CTGCCCGCCG ACGCCGACCC GGTGCACCAC GAGGGCGAGT TGGCGATCGT CATCGGCAGG CCGTGCAAGG ACGTGCCTGC CGCGCGTGCG GCCGAATTCA TCCTCGGCTA CACGATCGGC AACGACGTCT CCGCGCGTGA TCAGCAGCGC AAGGACGGGC AGTGGATGCG GGCGAAGGGG CACGACACGT TCTGCCCGCT GGGGCCGTGG ATCGTCACCG ATCTCGACCC GTCCGATGTG GAGCTGCGCA CCGAGGTCAA CGGACAGGTG CGCCAACGCA GCCGAACCTC GCTGCTGCTC CACGACATCG GGCGTCTCGT GGAGTGGACC TCGGCGGTGA TGACGCTGCT GCCGGGTGAC GTCATCCTGA CCGGGACCCC GGAGGGCGTC GGCCCCATCG AGGACGGCGA CACCGTCAGC ATCACCGTCG AGGGCATCGG CACACTCACC AATCCTGTTG TGCGTAAAGG GAAGAAA
Details for MysmA.00471.c.A1.GE28647
| HARVESTED ON: | 2/17/2010 |
| SEQUENCED ON: | 2/24/2010 |
| EXPECTED MW: | 29kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Few (1-10) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFVSIEG DGPDAVCKEI AEHPFGNPNF TGRSWPLADV RLLAPILASK VIAVGKNYAA HAEEMGGVAP EDPVIFLKPN TAIIGPNVPI QLPADADPVH HEGELAIVIG RPCKDVPAAR AAEFILGYTI GNDVSARDQQ RKDGQWMRAK GHDTFCPLGP WIVTDLDPSD VELRTEVNGQ VRQRSRTSLL LHDIGRLVEW TSAVMTLLPG DVILTGTPEG VGPIEDGDTV SITVEGIGTL TNPVVRKG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgct taggtcgaat tgccagtcct gacggcgtcg cattcgtgag catcgaaggc gacggaccag atgccgtctg caaagagatc gccgagcatc cgttcggaaa cccgaacttc accggacgca gctggccgct ggccgacgtc cgcctgctcg caccgattct cgccagcaag gtgatcgcgg tgggtaagaa ctacgccgcg cacgccgagg agatgggcgg tgtggcgccc gaggatccgg tgatcttcct caaaccgaac accgcgatca tcggtcccaa cgtgccgatc cagctgcccg ccgacgccga cccggtgcac cacgagggcg agttggcgat cgtcatcggc aggccgtgca aggacgtgcc tgccgcgcgt gcggccgaat tcatcctcgg ctacacgatc ggcaacgacg tctccgcgcg tgatcagcag cgcaaggacg ggcagtggat gcgggcgaag gggcacgaca cgttctgccc gctggggccg tggatcgtca ccgatctcga cccgtccgat gtggagctgc gcaccgaggt caacggacag gtgcgccaac gcagccgaac ctcgctgctg ctccacgaca tcgggcgtct cgtggagtgg acctcggcgg tgatgacgct gctgccgggt gacgtcatcc tgaccgggac cccggagggc gtcggcccca tcgaggacgg cgacaccgtc agcatcaccg tcgagggcat cggcacactc accaatcctg ttgtgcgtaa aggga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFVSIEG DGPDAVCKEI AEHPFGNPNF TGRSWPLADV RLLAPILASK VIAVGKNYAA HAEEMGGVAP EDPVIFLKPN TAIIGPNVPI QLPADADPVH HEGELAIVIG RPCKDVPAAR AAEFILGYTI GNDVSARDQQ RKDGQWMRAK GHDTFCPLGP WIVTDLDPSD VELRTEVNGQ VRQRSRTSLL LHDIGRLVEW TSAVMTLLPG DVILTGTPEG VGPIEDGDTV SITVEGIGTL TNPVVRKGKK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcttaggt cgaattgcca gtcctgacgg cgtcgcattc gtgagcatcg aaggcgacgg accagatgcc gtctgcaaag agatcgccga gcatccgttc ggaaacccga acttcaccgg acgcagctgg ccgctggccg acgtccgcct gctcgcaccg attctcgcca gcaaggtgat cgcggtgggt aagaactacg ccgcgcacgc cgaggagatg ggcggtgtgg cgcccgagga tccggtgatc ttcctcaaac cgaacaccgc gatcatcggt cccaacgtgc cgatccagct gcccgccgac gccgacccgg tgcaccacga gggcgagttg gcgatcgtca tcggcaggcc gtgcaaggac gtgcctgccg cgcgtgcggc cgaattcatc ctcggctaca cgatcggcaa cgacgtctcc gcgcgtgatc agcagcgcaa ggacgggcag tggatgcggg cgaaggggca cgacacgttc tgcccgctgg ggccgtggat cgtcaccgat ctcgacccgt ccgatgtgga gctgcgcacc gaggtcaacg gacaggtgcg ccaacgcagc cgaacctcgc tgctgctcca cgacatcggg cgtctcgtgg agtggacctc ggcggtgatg acgctgctgc cgggtgacgt catcctgacc gggaccccgg agggcgtcgg ccccatcgag gacggcgaca ccgtcagcat caccgtcgag ggcatcggca cactcaccaa tcctgttgtg cgtaaaggga agaaaaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MysmA.00471.c.A1.PS00549
| PURIFICATION DATe: | 4/5/2010 |
| CONCENTRATION: | 101.04mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 99 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFVSIEG DGPDAVCKEI AEHPFGNPNF TGRSWPLADV RLLAPILASK VIAVGKNYAA HAEEMGGVAP EDPVIFLKPN TAIIGPNVPI QLPADADPVH HEGELAIVIG RPCKDVPAAR AAEFILGYTI GNDVSARDQQ RKDGQWMRAK GHDTFCPLGP WIVTDLDPSD VELRTEVNGQ VRQRSRTSLL LHDIGRLVEW TSAVMTLLPG DVILTGTPEG VGPIEDGDTV SITVEGIGTL TNPVVRKG
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgct taggtcgaat tgccagtcct gacggcgtcg cattcgtgag catcgaaggc gacggaccag atgccgtctg caaagagatc gccgagcatc cgttcggaaa cccgaacttc accggacgca gctggccgct ggccgacgtc cgcctgctcg caccgattct cgccagcaag gtgatcgcgg tgggtaagaa ctacgccgcg cacgccgagg agatgggcgg tgtggcgccc gaggatccgg tgatcttcct caaaccgaac accgcgatca tcggtcccaa cgtgccgatc cagctgcccg ccgacgccga cccggtgcac cacgagggcg agttggcgat cgtcatcggc aggccgtgca aggacgtgcc tgccgcgcgt gcggccgaat tcatcctcgg ctacacgatc ggcaacgacg tctccgcgcg tgatcagcag cgcaaggacg ggcagtggat gcgggcgaag gggcacgaca cgttctgccc gctggggccg tggatcgtca ccgatctcga cccgtccgat gtggagctgc gcaccgaggt caacggacag gtgcgccaac gcagccgaac ctcgctgctg ctccacgaca tcgggcgtct cgtggagtgg acctcggcgg tgatgacgct gctgccgggt gacgtcatcc tgaccgggac cccggagggc gtcggcccca tcgaggacgg cgacaccgtc agcatcaccg tcgagggcat cggcacactc accaatcctg ttgtgcgtaa aggga
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRLGRIASP DGVAFVSIEG DGPDAVCKEI AEHPFGNPNF TGRSWPLADV RLLAPILASK VIAVGKNYAA HAEEMGGVAP EDPVIFLKPN TAIIGPNVPI QLPADADPVH HEGELAIVIG RPCKDVPAAR AAEFILGYTI GNDVSARDQQ RKDGQWMRAK GHDTFCPLGP WIVTDLDPSD VELRTEVNGQ VRQRSRTSLL LHDIGRLVEW TSAVMTLLPG DVILTGTPEG VGPIEDGDTV SITVEGIGTL TNPVVRKGKK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGCTTAGGT CGAATTGCCA GTCCTGACGG CGTCGCATTC GTGAGCATCG AAGGCGACGG ACCAGATGCC GTCTGCAAAG AGATCGCCGA GCATCCGTTC GGAAACCCGA ACTTCACCGG ACGCAGCTGG CCGCTGGCCG ACGTCCGCCT GCTCGCACCG ATTCTCGCCA GCAAGGTGAT CGCGGTGGGT AAGAACTACG CCGCGCACGC CGAGGAGATG GGCGGTGTGG CGCCCGAGGA TCCGGTGATC TTCCTCAAAC CGAACACCGC GATCATCGGT CCCAACGTGC CGATCCAGCT GCCCGCCGAC GCCGACCCGG TGCACCACGA GGGCGAGTTG GCGATCGTCA TCGGCAGGCC GTGCAAGGAC GTGCCTGCCG CGCGTGCGGC CGAATTCATC CTCGGCTACA CGATCGGCAA CGACGTCTCC GCGCGTGATC AGCAGCGCAA GGACGGGCAG TGGATGCGGG CGAAGGGGCA CGACACGTTC TGCCCGCTGG GGCCGTGGAT CGTCACCGAT CTCGACCCGT CCGATGTGGA GCTGCGCACC GAGGTCAACG GACAGGTGCG CCAACGCAGC CGAACCTCGC TGCTGCTCCA CGACATCGGG CGTCTCGTGG AGTGGACCTC GGCGGTGATG ACGCTGCTGC CGGGTGACGT CATCCTGACC GGGACCCCGG AGGGCGTCGG CCCCATCGAG GACGGCGACA CCGTCAGCAT CACCGTCGAG GGCATCGGCA CACTCACCAA TCCTGTTGTG CGTAAAGGGA AGAAAAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA