MysmA.00458.d

Acyl-CoA thioesterase II

CENTER ID: MysmA.00458.d
ORGANISM: Mycobacterium smegmatis ATCC 700084 / mc(2)155
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MysmA.00458.d.A1.GE28643 Full length( MysmA.00458.d ) 1 287
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|246196.19.peg.3190
RefSeq: YP_887542.1
UniProt: A0QXA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQADFEELL AVLDLERVGE DLFIGSHPSK NPVRTFGGQM TAQAFVAAGR SLEHKLSPSA LSAHFIAGGD PEKDLEFHIV RLRDERRFAN RRVDVMQNGE LLTTVMISYM NGGRGLEHNV PAPDVPRPET LPKIDELLRG YEETVPLFVN ALRPIEWRYT NDPAWVMRDK GGKLDHNRVW LKTEGEMPDD PVLHGAALVY SSDTTVLDSI ITTHGLSWGF DRIFAVTMNH SVWFHRPIRF DEWVLYSTTS PVAAESRGLG TGHYFDASGQ LVATVVQEGI VKYFPGK
NT Sequence
GTGTCACAGG CCGACTTCGA GGAACTACTG GCGGTTCTCG ACCTCGAACG GGTCGGTGAG GATCTCTTCA TCGGGTCGCA TCCCAGCAAG AACCCGGTGC GCACGTTCGG CGGTCAGATG ACCGCGCAGG CGTTCGTCGC CGCGGGGCGC TCGCTTGAGC ACAAGCTCTC GCCGAGCGCC CTGTCGGCGC ATTTCATCGC AGGCGGCGAC CCGGAGAAGG ACCTGGAGTT CCACATCGTC CGGCTCCGGG ACGAGCGCCG GTTCGCCAAT CGCCGGGTGG ACGTCATGCA GAACGGCGAA CTGCTGACCA CGGTGATGAT CTCGTACATG AACGGTGGCC GCGGCCTCGA GCACAATGTG CCCGCGCCCG ACGTGCCACG TCCGGAGACG CTGCCCAAGA TCGACGAATT GCTGCGCGGC TACGAGGAAA CCGTGCCGTT GTTCGTCAAC GCGCTGCGTC CGATCGAGTG GCGGTACACC AACGATCCGG CCTGGGTCAT GCGCGACAAG GGCGGAAAAC TCGACCACAA CCGCGTCTGG CTCAAGACCG AGGGCGAGAT GCCCGACGAT CCGGTGCTGC ACGGCGCGGC GCTGGTCTAC TCGTCGGACA CCACGGTGCT CGACTCGATC ATCACCACGC ACGGATTGTC CTGGGGTTTC GACCGGATCT TCGCGGTGAC CATGAACCAC AGCGTGTGGT TCCACCGGCC CATCAGGTTC GACGAGTGGG TGCTGTACTC GACCACCTCA CCCGTGGCCG CCGAATCGCG CGGTCTGGGC ACCGGACACT ACTTCGACGC GTCCGGGCAA CTGGTGGCCA CCGTGGTGCA GGAAGGCATC GTCAAGTACT TCCCCGGCAA G
Details for MysmA.00458.d.A1.GE28643
HARVESTED ON: 2/17/2010
SEQUENCED ON: 2/24/2010
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 92
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQADFEE LLAVLDLERV GEDLFIGSHP SKNPVRTFGG QMTAQAFVAA GRSLEHKLSP SALSAHFIAG GDPEKDLEFH IVRLRDERRF ANRRVDVMQN GELLTTVMIS YMNGGRGLEH NVPAPDVPRP ETLPKIDELL RGYEETVPLF VNALRPIEWR YTNDPAWVMR DKGGKLDHNR VWLKTEGEMP DDPVLHGAAL VYSSDTTVLD SIITTHGLSX XFDRIFAVTM NHSVWFXRPI RFDEWVLYST XSPVAAXSRG LGTGXY
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgt cacaggccga cttcgaggaa ctactggcgg ttctcgacct cgaacgggtc ggtgaggatc tcttcatcgg gtcgcatccc agcaagaacc cggtgcgcac gttcggcggt cagatgaccg cgcaggcgtt cgtcgccgcg gggcgctcgc ttgagcacaa gctctcgccg agcgccctgt cggcgcattt catcgcaggc ggcgacccgg agaaggacct ggagttccac atcgtccggc tccgggacga gcgccggttc gccaatcgcc gggtggacgt catgcagaac ggcgaactgc tgaccacggt gatgatctcg tacatgaacg gtggccgcgg cctcgagcac aatgtgcccg cgcccgacgt gccacgtccg gagacgctgc ccaagatcga cgaattgctg cgcggctacg aggaaaccgt gccgttgttc gtcaacgcgc tgcgtccgat cgagtggcgg tacaccaacg atccggcctg ggtcatgcgc gacaagggcg gaaaactcga ccacaaccgc gtctggctca agaccgaggg cgagatgccc gacgatccgg tgctgcacgg cgcggcgctg gtctactcgt cggacaccac ggtgctcgac tcgatcatca ccacgcacgg attgtcctgn ngtttcgacc ggatcttcgc ggtgaccatg aaccacagcg tgtggttccn ccggcccatc aggttcgacg agtgggtgct gtactcnacc ncctcacccg tggccgccna atcncgcggt ctgggcaccg gannctactt c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSQADFEE LLAVLDLERV GEDLFIGSHP SKNPVRTFGG QMTAQAFVAA GRSLEHKLSP SALSAHFIAG GDPEKDLEFH IVRLRDERRF ANRRVDVMQN GELLTTVMIS YMNGGRGLEH NVPAPDVPRP ETLPKIDELL RGYEETVPLF VNALRPIEWR YTNDPAWVMR DKGGKLDHNR VWLKTEGEMP DDPVLHGAAL VYSSDTTVLD SIITTHGLSW GFDRIFAVTM NHSVWFHRPI RFDEWVLYST TSPVAAESRG LGTGHYFDAS GQLVATVVQE GIVKYFPGK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcacag gccgacttcg aggaactact ggcggttctc gacctcgaac gggtcggtga ggatctcttc atcgggtcgc atcccagcaa gaacccggtg cgcacgttcg gcggtcagat gaccgcgcag gcgttcgtcg ccgcggggcg ctcgcttgag cacaagctct cgccgagcgc cctgtcggcg catttcatcg caggcggcga cccggagaag gacctggagt tccacatcgt ccggctccgg gacgagcgcc ggttcgccaa tcgccgggtg gacgtcatgc agaacggcga actgctgacc acggtgatga tctcgtacat gaacggtggc cgcggcctcg agcacaatgt gcccgcgccc gacgtgccac gtccggagac gctgcccaag atcgacgaat tgctgcgcgg ctacgaggaa accgtgccgt tgttcgtcaa cgcgctgcgt ccgatcgagt ggcggtacac caacgatccg gcctgggtca tgcgcgacaa gggcggaaaa ctcgaccaca accgcgtctg gctcaagacc gagggcgaga tgcccgacga tccggtgctg cacggcgcgg cgctggtcta ctcgtcggac accacggtgc tcgactcgat catcaccacg cacggattgt cctggggttt cgaccggatc ttcgcggtga ccatgaacca cagcgtgtgg ttccaccggc ccatcaggtt cgacgagtgg gtgctgtact cgaccacctc acccgtggcc gccgaatcgc gcggtctggg caccggacac tacttcgacg cgtccgggca actggtggcc accgtggtgc aggaaggcat cgtcaagtac ttccccggca agaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa